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The map label for this gene is trmD [H]

Identifier: 119899190

GI number: 119899190

Start: 3182723

End: 3183535

Strand: Direct

Name: trmD [H]

Synonym: azo2900

Alternate gene names: 119899190

Gene position: 3182723-3183535 (Clockwise)

Preceding gene: 119899189

Following gene: 119899191

Centisome position: 72.73

GC content: 66.17

Gene sequence:

>813_bases
GTGGCAGAAAGACTGGTGACAGCGACAACCGGCGGAAAACGCCGCTTCGACGTGGTGACGCTGTTTCCGGAGATGTTCGC
CGCGCTCACCGCAAGCGGCATCACCCGGCGCGCGCGCGATCGTGGCTTGTACGACATCGGTTTCCACAACCCGCGCGACT
TTGCCACCGATGCGCACCGTACGGTCGATGACCGACCCTACGGCGGCGGTCCCGGCATGGTCATGCTGGCCGAACCGCTG
GAGAAATCCATCGGCCAGGCGCGCGCGGCACAGCAGGCGGCACTGGGCACGAGCGGCCCGGTGATCTACCTGTCGCCGCA
GGGCCGTACGCTGGATCACGGCCGGGTGCAGGAACTTGCCGCCGCGCCAGGAATGATCCTGCTGTGCGGCCGCTACGAAG
GCATCGACCAGCGGCTGATAGACCGCTGCGTGGATGAGGAAATCTCGCTCGGCGACTTCGTCCTCTCCGGCGGCGAACTG
CCGGCGATGGTGTTGCTCGACGCAGTGATCCGGCAACTGCCGGGGGCCTTGAATGATGCCGATTCGGCGGTGGAGGATTC
CTTCGTCGACGGCCTGCTGGATTGCCAGCACTACACCCGCCCTGAGATTTACGAGGGCGAACGGGTGCCGGAAGTCCTGC
TCTCGGGCAACCATGCGCTGATCCGGCGCTGGCGCCTGAAGCAGGCGCTGGGGCGTACCGCGCTGCGCCGCCCCGATCTG
CTCGCCGCGCGTCGGCTGAGCAAGGAAGAATTGAACCTGCTTGAGGAATTCAAGCGGGAACAAGCTGGAGCCGAGGACGG
CAACGCCGTCTGA

Upstream 100 bases:

>100_bases
ATGTACTGCAGGTCGAAGACGGCGAGACGGAGCGGCTCATTCCCTTCGTGGCGGCCTATGTACTTGATGTAGACCTCGCG
GCCCGGCGCATCCGGGTGGC

Downstream 100 bases:

>100_bases
ACGCTCGATCACAAACCGCGTGCATCAGGTGGCGACCTGCTCTGCCGCAAGGCCAAGTGCCATTGACCATACTGGAGTCA
ACGAAATGAACCTGATTCAG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MAERLVTATTGGKRRFDVVTLFPEMFAALTASGITRRARDRGLYDIGFHNPRDFATDAHRTVDDRPYGGGPGMVMLAEPL
EKSIGQARAAQQAALGTSGPVIYLSPQGRTLDHGRVQELAAAPGMILLCGRYEGIDQRLIDRCVDEEISLGDFVLSGGEL
PAMVLLDAVIRQLPGALNDADSAVEDSFVDGLLDCQHYTRPEIYEGERVPEVLLSGNHALIRRWRLKQALGRTALRRPDL
LAARRLSKEELNLLEEFKREQAGAEDGNAV

Sequences:

>Translated_270_residues
MAERLVTATTGGKRRFDVVTLFPEMFAALTASGITRRARDRGLYDIGFHNPRDFATDAHRTVDDRPYGGGPGMVMLAEPL
EKSIGQARAAQQAALGTSGPVIYLSPQGRTLDHGRVQELAAAPGMILLCGRYEGIDQRLIDRCVDEEISLGDFVLSGGEL
PAMVLLDAVIRQLPGALNDADSAVEDSFVDGLLDCQHYTRPEIYEGERVPEVLLSGNHALIRRWRLKQALGRTALRRPDL
LAARRLSKEELNLLEEFKREQAGAEDGNAV
>Mature_269_residues
AERLVTATTGGKRRFDVVTLFPEMFAALTASGITRRARDRGLYDIGFHNPRDFATDAHRTVDDRPYGGGPGMVMLAEPLE
KSIGQARAAQQAALGTSGPVIYLSPQGRTLDHGRVQELAAAPGMILLCGRYEGIDQRLIDRCVDEEISLGDFVLSGGELP
AMVLLDAVIRQLPGALNDADSAVEDSFVDGLLDCQHYTRPEIYEGERVPEVLLSGNHALIRRWRLKQALGRTALRRPDLL
AARRLSKEELNLLEEFKREQAGAEDGNAV

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=245, Percent_Identity=60.4081632653061, Blast_Score=285, Evalue=2e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 29591; Mature: 29460

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERLVTATTGGKRRFDVVTLFPEMFAALTASGITRRARDRGLYDIGFHNPRDFATDAHR
CCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHH
TVDDRPYGGGPGMVMLAEPLEKSIGQARAAQQAALGTSGPVIYLSPQGRTLDHGRVQELA
CCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHH
AAPGMILLCGRYEGIDQRLIDRCVDEEISLGDFVLSGGELPAMVLLDAVIRQLPGALNDA
HCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCH
DSAVEDSFVDGLLDCQHYTRPEIYEGERVPEVLLSGNHALIRRWRLKQALGRTALRRPDL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHH
LAARRLSKEELNLLEEFKREQAGAEDGNAV
HHHHHCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AERLVTATTGGKRRFDVVTLFPEMFAALTASGITRRARDRGLYDIGFHNPRDFATDAHR
CCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHH
TVDDRPYGGGPGMVMLAEPLEKSIGQARAAQQAALGTSGPVIYLSPQGRTLDHGRVQELA
CCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHH
AAPGMILLCGRYEGIDQRLIDRCVDEEISLGDFVLSGGELPAMVLLDAVIRQLPGALNDA
HCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCH
DSAVEDSFVDGLLDCQHYTRPEIYEGERVPEVLLSGNHALIRRWRLKQALGRTALRRPDL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHH
LAARRLSKEELNLLEEFKREQAGAEDGNAV
HHHHHCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA