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The map label for this gene is tyrS [H]

Identifier: 119899046

GI number: 119899046

Start: 3038104

End: 3039303

Strand: Reverse

Name: tyrS [H]

Synonym: azo2756

Alternate gene names: 119899046

Gene position: 3039303-3038104 (Counterclockwise)

Preceding gene: 119899048

Following gene: 119899045

Centisome position: 69.45

GC content: 63.83

Gene sequence:

>1200_bases
ATGACTGACGTCCAGGCCGCGATCGAGCTGATCAAGCGTGGCGCAGACGAGCTGCTGATCGAGGCCGAACTCGTCGAGAA
ACTGAAGTCCGGCCGTCCGCTGAGGATCAAGGCGGGCTTCGACCCCACCGCTCCCGACCTCCACCTCGGTCATACGGTCC
TGATCAACAAGCTGCGCCATTTCCAGGAGCTGGGGCATCAGGTCCTGTTCCTCATCGGCGACTTCACCGGCATGATCGGC
GACCCGAGCGGCAAGAATGCAACCCGCCCGCCGCTGTCGCGCGAGCAGATTCTCGAGAACGCGAAGACCTATCAGGATCA
GGTGTTCAAGATCCTGGACCCGGCAAAGACCGAAATCTGCTTCAACTCCGCATGGATGGAAGGGCTCGGTTCGGCCGGGA
TGATCCGCCTGGCCGCGCAGCAGACGGTGGCACGCATGCTGGAACGCGACGACTTCTCCAAGCGTTACGCCAACAACCAG
TCGATCGCCATCCACGAGTTCCTGTATCCGCTGTGCCAGGGCTACGACTCGGTGGCCATGAAGGCCGATGTCGAACTCGG
CGGCACCGACCAGCGCTTCAACCTGCTGATGGGGCGCGAGCTGCAGAAGCACTACGGGCAGGCACCGCAGTGCGTGCTGA
TGATGCCGCTGCTGGAGGGGCTGGACGGCGTCAACAAGATGTCGAAGTCGCTCGGCAACTACATCGGCATTGCCGAGGCG
CCGAAGGAGATCTTCGGCAAGACGATGTCGGTGTCGGACCAGCTGATGTGGCGTTACTACGATCTGCTGTCCTTCCGGTC
GACGGCTGAGATCGCGCGGCTGCGCAGTGAGGTCGAAGGCGGTCGCAACCCGCGCGACGTCAAGGTGATGCTCGCGCTCG
AACTCGTCGCGCGCTTTCATGGTCAGTTGGCGGCCGACGAGGCGCTGGCCGATTTCGAGGCGCGTTTCCAGCGCAATGCC
ATCCCCGATGACCTGCCCGAGGTAAGGGTGAGCGTTGGCGCCGAAGGCTTGCCGGTGTTCCAGGTCGTCAAGCAGGCAGG
GCTGACCGGCACCACTTCCGAGGCGATCCGCATGATCGACCAGGGCGCGGTGCGCCTCAACGGCGACCGTGTGGAGGACA
AGGGGCTGGTGCTGGCAGCGGGCGATACCGTCGTGCTGCAGGTGGGCAAGCGCAAGTTCGCGGCGGTCGTGCTCGAATGA

Upstream 100 bases:

>100_bases
ATCCTGCTTTTCCTGACCAGCATTGGCTTTTTCGGGCAAAAAACTCCCGCGAGTCTATCAAAAATCGCGGGTGATTCGCG
TTTGAATTGGAGAGATTTTG

Downstream 100 bases:

>100_bases
CCGCAGACGCCCGGGCCTCCCGCTCGATCGGCGTGTTCGATTCGGGCATCGGCGGCCTTACCGTGGTGCGGGCGCTGATG
GAGCGCCTGCCCTTCGAGAA

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTDVQAAIELIKRGADELLIEAELVEKLKSGRPLRIKAGFDPTAPDLHLGHTVLINKLRHFQELGHQVLFLIGDFTGMIG
DPSGKNATRPPLSREQILENAKTYQDQVFKILDPAKTEICFNSAWMEGLGSAGMIRLAAQQTVARMLERDDFSKRYANNQ
SIAIHEFLYPLCQGYDSVAMKADVELGGTDQRFNLLMGRELQKHYGQAPQCVLMMPLLEGLDGVNKMSKSLGNYIGIAEA
PKEIFGKTMSVSDQLMWRYYDLLSFRSTAEIARLRSEVEGGRNPRDVKVMLALELVARFHGQLAADEALADFEARFQRNA
IPDDLPEVRVSVGAEGLPVFQVVKQAGLTGTTSEAIRMIDQGAVRLNGDRVEDKGLVLAAGDTVVLQVGKRKFAAVVLE

Sequences:

>Translated_399_residues
MTDVQAAIELIKRGADELLIEAELVEKLKSGRPLRIKAGFDPTAPDLHLGHTVLINKLRHFQELGHQVLFLIGDFTGMIG
DPSGKNATRPPLSREQILENAKTYQDQVFKILDPAKTEICFNSAWMEGLGSAGMIRLAAQQTVARMLERDDFSKRYANNQ
SIAIHEFLYPLCQGYDSVAMKADVELGGTDQRFNLLMGRELQKHYGQAPQCVLMMPLLEGLDGVNKMSKSLGNYIGIAEA
PKEIFGKTMSVSDQLMWRYYDLLSFRSTAEIARLRSEVEGGRNPRDVKVMLALELVARFHGQLAADEALADFEARFQRNA
IPDDLPEVRVSVGAEGLPVFQVVKQAGLTGTTSEAIRMIDQGAVRLNGDRVEDKGLVLAAGDTVVLQVGKRKFAAVVLE
>Mature_398_residues
TDVQAAIELIKRGADELLIEAELVEKLKSGRPLRIKAGFDPTAPDLHLGHTVLINKLRHFQELGHQVLFLIGDFTGMIGD
PSGKNATRPPLSREQILENAKTYQDQVFKILDPAKTEICFNSAWMEGLGSAGMIRLAAQQTVARMLERDDFSKRYANNQS
IAIHEFLYPLCQGYDSVAMKADVELGGTDQRFNLLMGRELQKHYGQAPQCVLMMPLLEGLDGVNKMSKSLGNYIGIAEAP
KEIFGKTMSVSDQLMWRYYDLLSFRSTAEIARLRSEVEGGRNPRDVKVMLALELVARFHGQLAADEALADFEARFQRNAI
PDDLPEVRVSVGAEGLPVFQVVKQAGLTGTTSEAIRMIDQGAVRLNGDRVEDKGLVLAAGDTVVLQVGKRKFAAVVLE

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=411, Percent_Identity=25.3041362530414, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI1787925, Length=430, Percent_Identity=27.4418604651163, Blast_Score=129, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI25145802, Length=437, Percent_Identity=25.1716247139588, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI20130383, Length=405, Percent_Identity=25.9259259259259, Blast_Score=121, Evalue=7e-28,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 44084; Mature: 43953

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVQAAIELIKRGADELLIEAELVEKLKSGRPLRIKAGFDPTAPDLHLGHTVLINKLRH
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHH
FQELGHQVLFLIGDFTGMIGDPSGKNATRPPLSREQILENAKTYQDQVFKILDPAKTEIC
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHH
FNSAWMEGLGSAGMIRLAAQQTVARMLERDDFSKRYANNQSIAIHEFLYPLCQGYDSVAM
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHCCCCHHEE
KADVELGGTDQRFNLLMGRELQKHYGQAPQCVLMMPLLEGLDGVNKMSKSLGNYIGIAEA
EECCEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
PKEIFGKTMSVSDQLMWRYYDLLSFRSTAEIARLRSEVEGGRNPRDVKVMLALELVARFH
CHHHHCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
GQLAADEALADFEARFQRNAIPDDLPEVRVSVGAEGLPVFQVVKQAGLTGTTSEAIRMID
CCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHH
QGAVRLNGDRVEDKGLVLAAGDTVVLQVGKRKFAAVVLE
CCCEEECCCEECCCCEEEEECCEEEEEECCCEEEEEECC
>Mature Secondary Structure 
TDVQAAIELIKRGADELLIEAELVEKLKSGRPLRIKAGFDPTAPDLHLGHTVLINKLRH
CCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHH
FQELGHQVLFLIGDFTGMIGDPSGKNATRPPLSREQILENAKTYQDQVFKILDPAKTEIC
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHH
FNSAWMEGLGSAGMIRLAAQQTVARMLERDDFSKRYANNQSIAIHEFLYPLCQGYDSVAM
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHCCCCHHEE
KADVELGGTDQRFNLLMGRELQKHYGQAPQCVLMMPLLEGLDGVNKMSKSLGNYIGIAEA
EECCEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
PKEIFGKTMSVSDQLMWRYYDLLSFRSTAEIARLRSEVEGGRNPRDVKVMLALELVARFH
CHHHHCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
GQLAADEALADFEARFQRNAIPDDLPEVRVSVGAEGLPVFQVVKQAGLTGTTSEAIRMID
CCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHH
QGAVRLNGDRVEDKGLVLAAGDTVVLQVGKRKFAAVVLE
CCCEEECCCEECCCCEEEEECCEEEEEECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA