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The map label for this gene is ycdT [H]

Identifier: 119898940

GI number: 119898940

Start: 2926624

End: 2928333

Strand: Reverse

Name: ycdT [H]

Synonym: azo2649

Alternate gene names: 119898940

Gene position: 2928333-2926624 (Counterclockwise)

Preceding gene: 119898941

Following gene: 119898939

Centisome position: 66.92

GC content: 69.94

Gene sequence:

>1710_bases
ATGAACGTCCTGCCCCGCTCGCTCCAGAACCAGCTTGCCGCCCTGTTCGGCGCACTGGCGCTGCTCGTGGGGCTGCCGAC
CTATTTCTACATCGACCACATCCATGCCCAGCAATTGGTGCGCGACCGCGGCCAGGCGCTGCACGAACTGGCTGGCGCGG
TGGCGATGGTGGTCGCCGAGAACCTCAACGAGCGCAAGCGCGAGATCGACCTGCTGGCGCAGCGCCCCAGCTTCCGCCGC
GGGCCGCTGGATGGGGCCGACCTGCGCACCGGCATCGAGGCGCTGAAGAACTCCTACCCTCACTATTCATGGATCGGTCT
GGCGGACACCCAGGGCGTGGTGCGTGCCGCCTCGGGCGACATGCTGGTCGGCCAGAGCGTGGCGCAGCGGCCGTGGTTTG
GCGGCGGGCTGCTGGGCTCCTTCCTCGGCGACGTGCACGAGGCCGTGCTGCTGACCAAGCTGCTGCAGGTTCCGCCGGGC
GAGGGGCCGGTGCGCTTCATCGACTTCGCCGCGCCGGTGTATGACGAGCAGGGCGTCCTGCGCGGCGTGGTTGCGGCGCA
CGCGCACTGGCGCTGGGCGGCCGACGTGGTGGCCGCGGTGATGCCGCGCAACGCCGCCGAAAAGCAGATCGAGGTGTTCC
TGGTAAACCGCGACGGCAAGATCATCTTTCCCGAACGCGACCTGCCGGTCGATGCCGTTCCGGCGGCGGTGAGTACCCAC
ACCACGTACTCCTTCGACAACTGGGGCAGCGACAGCTCGTTTCTGAGTGCGCGGGTAAACGTGCGCGAACTCGTCGACGG
GCAGCCGCTGGGCTGGTCGGTGGTGGTGCGCCAGCCGCGCGAGCGCGCAATGGCGGACCTGCGCGCGCTGCAGGAACTCG
TGGTGTGCGTGGCGCTGGGCGCCGCGGCGGCATTTCTGGCGCTGGCGTGGTGGAGCGCCGGACGCATGAGTCGGCCGCTG
GAGCAACTCGCCGGGCTCGCCAAGCGCATCCAGCAAGGCGAGGAGCATCTCGCGCTCGAAGTGCGCGGCACCAGCGCGGA
AGTGGTGAAGCTGGTGGGAGCGATGCGCGGCATGGCCTCCACGCTGGTCAGCCGCAAGCACTCGCTCGAGCTGAGCAACC
TCGCGCTGGAGCAGAAGGTGGCCGAACGCACTGCCGAGCTGCAGCGCCTCAACGCTGAACTGGTGCAGCTTGCCCGGCGC
GACGCGCTGACCGGGCTGCCCAACCGGCTGGCGGCCAACGAGCAATTGACCGAGGAATTCCTGCGCATGAAGCGGTCCTT
GGTGCCCTATGCGGTGCTGATGGCCGACATCGACCTCTTCAAGCGCATCAACGATGGTCACGGCCATGCGGTGGGCGACG
CGGTGCTGCGCCACGTGGCGGGGGTGATCCGGTCGAGCCTGCGCATCACCGACTTCGTCGCCCGCGTCGGCGGCGAGGAG
TTCCTGGTGCTGTTGCCCAACACCGATATCGAGGCCGCGGCCGTGGTTGCCGAGAAGATCCGCGTAGCGGTGGAGGGGGC
GGCGGCGCCCGAGGTCGGCCAGGTGACGCTTTCCATCGGCGTCGGCCGCGCCGTGTCCGCCCACCCCAGCCCGGACGACG
CGGTGCGCCTGGCCGACGACGGCCTGTACGAGGCCAAGCGGCTGGGGCGCAACCGCATCAGCGTGGTCGGCGTTGCGCTG
GCCGGGGTGGCGGACGCCGGCGAAGCCTGA

Upstream 100 bases:

>100_bases
CCTTTGATTTCAAGCATCTTGCGGGGGAGGGTGGCAATAGTACTGAAGTCGTAGGGGTTAAACGGGTGTATAAGGGCGCA
CCGCCAATCCCGCGTCGCCC

Downstream 100 bases:

>100_bases
GCGGGCCGAAAGCCGTGGCGTCCGGTCCGCGCGGTTTGCCGCCGGAGCGCTGTTTCCCGGCCGCTGGCGCGGTGGCATCA
TCACCGTTTGCATGCGGACG

Product: GGDEF family protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 569; Mature: 569

Protein sequence:

>569_residues
MNVLPRSLQNQLAALFGALALLVGLPTYFYIDHIHAQQLVRDRGQALHELAGAVAMVVAENLNERKREIDLLAQRPSFRR
GPLDGADLRTGIEALKNSYPHYSWIGLADTQGVVRAASGDMLVGQSVAQRPWFGGGLLGSFLGDVHEAVLLTKLLQVPPG
EGPVRFIDFAAPVYDEQGVLRGVVAAHAHWRWAADVVAAVMPRNAAEKQIEVFLVNRDGKIIFPERDLPVDAVPAAVSTH
TTYSFDNWGSDSSFLSARVNVRELVDGQPLGWSVVVRQPRERAMADLRALQELVVCVALGAAAAFLALAWWSAGRMSRPL
EQLAGLAKRIQQGEEHLALEVRGTSAEVVKLVGAMRGMASTLVSRKHSLELSNLALEQKVAERTAELQRLNAELVQLARR
DALTGLPNRLAANEQLTEEFLRMKRSLVPYAVLMADIDLFKRINDGHGHAVGDAVLRHVAGVIRSSLRITDFVARVGGEE
FLVLLPNTDIEAAAVVAEKIRVAVEGAAAPEVGQVTLSIGVGRAVSAHPSPDDAVRLADDGLYEAKRLGRNRISVVGVAL
AGVADAGEA

Sequences:

>Translated_569_residues
MNVLPRSLQNQLAALFGALALLVGLPTYFYIDHIHAQQLVRDRGQALHELAGAVAMVVAENLNERKREIDLLAQRPSFRR
GPLDGADLRTGIEALKNSYPHYSWIGLADTQGVVRAASGDMLVGQSVAQRPWFGGGLLGSFLGDVHEAVLLTKLLQVPPG
EGPVRFIDFAAPVYDEQGVLRGVVAAHAHWRWAADVVAAVMPRNAAEKQIEVFLVNRDGKIIFPERDLPVDAVPAAVSTH
TTYSFDNWGSDSSFLSARVNVRELVDGQPLGWSVVVRQPRERAMADLRALQELVVCVALGAAAAFLALAWWSAGRMSRPL
EQLAGLAKRIQQGEEHLALEVRGTSAEVVKLVGAMRGMASTLVSRKHSLELSNLALEQKVAERTAELQRLNAELVQLARR
DALTGLPNRLAANEQLTEEFLRMKRSLVPYAVLMADIDLFKRINDGHGHAVGDAVLRHVAGVIRSSLRITDFVARVGGEE
FLVLLPNTDIEAAAVVAEKIRVAVEGAAAPEVGQVTLSIGVGRAVSAHPSPDDAVRLADDGLYEAKRLGRNRISVVGVAL
AGVADAGEA
>Mature_569_residues
MNVLPRSLQNQLAALFGALALLVGLPTYFYIDHIHAQQLVRDRGQALHELAGAVAMVVAENLNERKREIDLLAQRPSFRR
GPLDGADLRTGIEALKNSYPHYSWIGLADTQGVVRAASGDMLVGQSVAQRPWFGGGLLGSFLGDVHEAVLLTKLLQVPPG
EGPVRFIDFAAPVYDEQGVLRGVVAAHAHWRWAADVVAAVMPRNAAEKQIEVFLVNRDGKIIFPERDLPVDAVPAAVSTH
TTYSFDNWGSDSSFLSARVNVRELVDGQPLGWSVVVRQPRERAMADLRALQELVVCVALGAAAAFLALAWWSAGRMSRPL
EQLAGLAKRIQQGEEHLALEVRGTSAEVVKLVGAMRGMASTLVSRKHSLELSNLALEQKVAERTAELQRLNAELVQLARR
DALTGLPNRLAANEQLTEEFLRMKRSLVPYAVLMADIDLFKRINDGHGHAVGDAVLRHVAGVIRSSLRITDFVARVGGEE
FLVLLPNTDIEAAAVVAEKIRVAVEGAAAPEVGQVTLSIGVGRAVSAHPSPDDAVRLADDGLYEAKRLGRNRISVVGVAL
AGVADAGEA

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=165, Percent_Identity=43.030303030303, Blast_Score=123, Evalue=4e-29,
Organism=Escherichia coli, GI87081881, Length=175, Percent_Identity=38.8571428571429, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI145693134, Length=163, Percent_Identity=36.1963190184049, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI87082007, Length=169, Percent_Identity=40.2366863905325, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1786584, Length=183, Percent_Identity=33.879781420765, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1788381, Length=179, Percent_Identity=33.5195530726257, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787816, Length=186, Percent_Identity=33.8709677419355, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787802, Length=179, Percent_Identity=37.4301675977654, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787541, Length=173, Percent_Identity=35.2601156069364, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788085, Length=173, Percent_Identity=32.3699421965318, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081974, Length=168, Percent_Identity=29.1666666666667, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87081977, Length=167, Percent_Identity=31.1377245508982, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788956, Length=189, Percent_Identity=30.1587301587302, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787056, Length=125, Percent_Identity=33.6, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 61369; Mature: 61369

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS50885 HAMP ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLPRSLQNQLAALFGALALLVGLPTYFYIDHIHAQQLVRDRGQALHELAGAVAMVVAE
CCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLNERKREIDLLAQRPSFRRGPLDGADLRTGIEALKNSYPHYSWIGLADTQGVVRAASGD
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHEECCCC
MLVGQSVAQRPWFGGGLLGSFLGDVHEAVLLTKLLQVPPGEGPVRFIDFAAPVYDEQGVL
EEECCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHH
RGVVAAHAHWRWAADVVAAVMPRNAAEKQIEVFLVNRDGKIIFPERDLPVDAVPAAVSTH
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEECCCCCCCCCCCCCHHHCC
TTYSFDNWGSDSSFLSARVNVRELVDGQPLGWSVVVRQPRERAMADLRALQELVVCVALG
CEECCCCCCCCCHHHHHHCCHHHHHCCCCCCHHHEECCCHHHHHHHHHHHHHHHHHHHHH
AAAAFLALAWWSAGRMSRPLEQLAGLAKRIQQGEEHLALEVRGTSAEVVKLVGAMRGMAS
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHH
TLVSRKHSLELSNLALEQKVAERTAELQRLNAELVQLARRDALTGLPNRLAANEQLTEEF
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHH
LRMKRSLVPYAVLMADIDLFKRINDGHGHAVGDAVLRHVAGVIRSSLRITDFVARVGGEE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
FLVLLPNTDIEAAAVVAEKIRVAVEGAAAPEVGQVTLSIGVGRAVSAHPSPDDAVRLADD
EEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEECCC
GLYEAKRLGRNRISVVGVALAGVADAGEA
CHHHHHHCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNVLPRSLQNQLAALFGALALLVGLPTYFYIDHIHAQQLVRDRGQALHELAGAVAMVVAE
CCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLNERKREIDLLAQRPSFRRGPLDGADLRTGIEALKNSYPHYSWIGLADTQGVVRAASGD
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHEECCCC
MLVGQSVAQRPWFGGGLLGSFLGDVHEAVLLTKLLQVPPGEGPVRFIDFAAPVYDEQGVL
EEECCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHH
RGVVAAHAHWRWAADVVAAVMPRNAAEKQIEVFLVNRDGKIIFPERDLPVDAVPAAVSTH
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEECCCCCCCCCCCCCHHHCC
TTYSFDNWGSDSSFLSARVNVRELVDGQPLGWSVVVRQPRERAMADLRALQELVVCVALG
CEECCCCCCCCCHHHHHHCCHHHHHCCCCCCHHHEECCCHHHHHHHHHHHHHHHHHHHHH
AAAAFLALAWWSAGRMSRPLEQLAGLAKRIQQGEEHLALEVRGTSAEVVKLVGAMRGMAS
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHH
TLVSRKHSLELSNLALEQKVAERTAELQRLNAELVQLARRDALTGLPNRLAANEQLTEEF
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHH
LRMKRSLVPYAVLMADIDLFKRINDGHGHAVGDAVLRHVAGVIRSSLRITDFVARVGGEE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
FLVLLPNTDIEAAAVVAEKIRVAVEGAAAPEVGQVTLSIGVGRAVSAHPSPDDAVRLADD
EEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEECCC
GLYEAKRLGRNRISVVGVALAGVADAGEA
CHHHHHHCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]