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The map label for this gene is guaD [H]

Identifier: 119898514

GI number: 119898514

Start: 2435887

End: 2437182

Strand: Direct

Name: guaD [H]

Synonym: azo2223

Alternate gene names: 119898514

Gene position: 2435887-2437182 (Clockwise)

Preceding gene: 119898513

Following gene: 119898515

Centisome position: 55.66

GC content: 69.98

Gene sequence:

>1296_bases
ATGCCCCACGCCCTGCACGCCGTCCGCGGCGAAATCCTGCACTTCCTCGCGGACCCCGCCGACCGCTCCGACGCGCTCGT
CCATTTTCCGGACGGGCTGCTGCTGATCGAGAACGGCCACATCGCCGCGCTGGGGCCGGCGCCCGCCATGCTTGCAACGC
TGCCGCCCGAGCTGCCGGTCACCGACCATCGCGGCAAGCTCATCCTGCCCGGCTTCGTCGATACCCACGTCCACTACGCC
CAGACCGACATCATCGCCAGCCACGGCGAACAGCTGCTCGCGTGGCTGGAGCGCTACACCTTTCCGGCGGAAGCGCGCTT
TGCCGATCCGGCGCACGCCGCGGAGGTCGCCGCCTTTTTCTGCGACGAATTGCTGCGCAACGGCACCACCACCGCGCTGG
CCTTCGCCACCGTGCATCCCGCCTCGGTGGACGCGCTCTTAAGCGCGGCGCACGCGCGCCGCATGCGGATGATCGCCGGC
AAGGTGCTGATGGACCGCAACTGCCCGCCCGCGCTGCGCGACACCGCGCACGGCGGCTACGCCGCGTCGAAGGCGCTGAT
CGAGCGCTGGCACGGCACCGGCCGCCTCGGTTATGCCGTCACGCCGCGTTTCGCGCCCACCTCGAGCGACGCGCAGATGC
GGCTCGCCGGCCAGCTCTACGCCGAGTACCCGGACCTCCACCTGCAGTCGCACGTGGCAGAAAACCGCAGCGAGGTGGCG
TGGGCGGCGCAGCTCTACCCCACCGCGCGCAGCTATCTGGACGTATATGAAGGCTTCGGCCAGCTCGGGCCGCGAACGGT
GTTCGCGCATTGCATCTGGCTGGACGATGCCGACCGCGCGCGCATGGCAGCCACCGGCGCGGCGATCAGCTTCTGCCCGA
CCTCCAACCTCTTCCTCGGCTCCGGCCTGTTCGACCTCGACCGCGCGCATGCCCAGGGCGTGCGGGTGGGCATCGGCACC
GACGTTGGCGGCGGCACCAGCTTCTCGATGCTGCAGACCCTGAACGAGGCCTACAAGGTGTTGCAGCTCAACGGGCAGCA
CCTGTCGCCCGAACGCGCCTTCTACCTCGCCACCCTGGGTGGGGCGCGCAGCCTGTACCTGGATCACCGCATCGGCAATT
TCGCGCCGGGCAAGGAGGCCGATTTCGTTGTGCTCGACCCCGCCGCCACGCCGCTGCTGGCGCGCCGCGCCCTTGCCGGC
ACGCTGGCAGAACGCCTGTTCGTGCTGATGATGCTCGGCGACGACCGCTGCATTGCCGCCACCCACATTCTCGGAGAGCC
GGCATGGACGCGCTGA

Upstream 100 bases:

>100_bases
CGAGGCCCTCGCCACGCGCGTGCTCGAACTCCCCGCGCGGCCTTCGGCCTAGCGCCCCGGGCGCGCTATAGTCGGGGCTG
TTCCAGCCCGCTGCCGCCCG

Downstream 100 bases:

>100_bases
ACGAGCCGCGCATGCAGGTGTGGGTGGATGCCGACGCCTGCCCCGCGGTGATCAAGGACATCCTGTTCCGCGCCGCGCAG
CGCACCGGCCTGGCGCTGAC

Product: guanine deaminase

Products: NA

Alternate protein names: Guanase; Guanine aminase; Guanine aminohydrolase; GAH [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MPHALHAVRGEILHFLADPADRSDALVHFPDGLLLIENGHIAALGPAPAMLATLPPELPVTDHRGKLILPGFVDTHVHYA
QTDIIASHGEQLLAWLERYTFPAEARFADPAHAAEVAAFFCDELLRNGTTTALAFATVHPASVDALLSAAHARRMRMIAG
KVLMDRNCPPALRDTAHGGYAASKALIERWHGTGRLGYAVTPRFAPTSSDAQMRLAGQLYAEYPDLHLQSHVAENRSEVA
WAAQLYPTARSYLDVYEGFGQLGPRTVFAHCIWLDDADRARMAATGAAISFCPTSNLFLGSGLFDLDRAHAQGVRVGIGT
DVGGGTSFSMLQTLNEAYKVLQLNGQHLSPERAFYLATLGGARSLYLDHRIGNFAPGKEADFVVLDPAATPLLARRALAG
TLAERLFVLMMLGDDRCIAATHILGEPAWTR

Sequences:

>Translated_431_residues
MPHALHAVRGEILHFLADPADRSDALVHFPDGLLLIENGHIAALGPAPAMLATLPPELPVTDHRGKLILPGFVDTHVHYA
QTDIIASHGEQLLAWLERYTFPAEARFADPAHAAEVAAFFCDELLRNGTTTALAFATVHPASVDALLSAAHARRMRMIAG
KVLMDRNCPPALRDTAHGGYAASKALIERWHGTGRLGYAVTPRFAPTSSDAQMRLAGQLYAEYPDLHLQSHVAENRSEVA
WAAQLYPTARSYLDVYEGFGQLGPRTVFAHCIWLDDADRARMAATGAAISFCPTSNLFLGSGLFDLDRAHAQGVRVGIGT
DVGGGTSFSMLQTLNEAYKVLQLNGQHLSPERAFYLATLGGARSLYLDHRIGNFAPGKEADFVVLDPAATPLLARRALAG
TLAERLFVLMMLGDDRCIAATHILGEPAWTR
>Mature_430_residues
PHALHAVRGEILHFLADPADRSDALVHFPDGLLLIENGHIAALGPAPAMLATLPPELPVTDHRGKLILPGFVDTHVHYAQ
TDIIASHGEQLLAWLERYTFPAEARFADPAHAAEVAAFFCDELLRNGTTTALAFATVHPASVDALLSAAHARRMRMIAGK
VLMDRNCPPALRDTAHGGYAASKALIERWHGTGRLGYAVTPRFAPTSSDAQMRLAGQLYAEYPDLHLQSHVAENRSEVAW
AAQLYPTARSYLDVYEGFGQLGPRTVFAHCIWLDDADRARMAATGAAISFCPTSNLFLGSGLFDLDRAHAQGVRVGIGTD
VGGGTSFSMLQTLNEAYKVLQLNGQHLSPERAFYLATLGGARSLYLDHRIGNFAPGKEADFVVLDPAATPLLARRALAGT
LAERLFVLMMLGDDRCIAATHILGEPAWTR

Specific function: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia [H]

COG id: COG0402

COG function: function code FR; Cytosine deaminase and related metal-dependent hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATZ/TRZ family [H]

Homologues:

Organism=Homo sapiens, GI4758426, Length=386, Percent_Identity=36.2694300518135, Blast_Score=221, Evalue=1e-57,
Organism=Escherichia coli, GI1789249, Length=433, Percent_Identity=54.7344110854504, Blast_Score=484, Evalue=1e-138,
Organism=Saccharomyces cerevisiae, GI6319963, Length=352, Percent_Identity=34.9431818181818, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24643849, Length=429, Percent_Identity=33.7995337995338, Blast_Score=207, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR014311
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.4.3 [H]

Molecular weight: Translated: 46604; Mature: 46472

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHALHAVRGEILHFLADPADRSDALVHFPDGLLLIENGHIAALGPAPAMLATLPPELPV
CCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCEEEEECCCEEEECCCCHHHHCCCCCCCC
TDHRGKLILPGFVDTHVHYAQTDIIASHGEQLLAWLERYTFPAEARFADPAHAAEVAAFF
CCCCCCEEECCHHHCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
CDELLRNGTTTALAFATVHPASVDALLSAAHARRMRMIAGKVLMDRNCPPALRDTAHGGY
HHHHHHCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCH
AASKALIERWHGTGRLGYAVTPRFAPTSSDAQMRLAGQLYAEYPDLHLQSHVAENRSEVA
HHHHHHHHHHCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHH
WAAQLYPTARSYLDVYEGFGQLGPRTVFAHCIWLDDADRARMAATGAAISFCPTSNLFLG
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCHHHHHCCCEEEECCCCCEEEC
SGLFDLDRAHAQGVRVGIGTDVGGGTSFSMLQTLNEAYKVLQLNGQHLSPERAFYLATLG
CCCHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHEEEECCCCCCCCCEEEEEEEC
GARSLYLDHRIGNFAPGKEADFVVLDPAATPLLARRALAGTLAERLFVLMMLGDDRCIAA
CCEEEEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHEEEEECCCCEEEE
THILGEPAWTR
HHHHCCCCCCC
>Mature Secondary Structure 
PHALHAVRGEILHFLADPADRSDALVHFPDGLLLIENGHIAALGPAPAMLATLPPELPV
CCHHHHHHHHHHHHHCCCCCCCCCEEECCCCEEEEECCCEEEECCCCHHHHCCCCCCCC
TDHRGKLILPGFVDTHVHYAQTDIIASHGEQLLAWLERYTFPAEARFADPAHAAEVAAFF
CCCCCCEEECCHHHCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
CDELLRNGTTTALAFATVHPASVDALLSAAHARRMRMIAGKVLMDRNCPPALRDTAHGGY
HHHHHHCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCH
AASKALIERWHGTGRLGYAVTPRFAPTSSDAQMRLAGQLYAEYPDLHLQSHVAENRSEVA
HHHHHHHHHHCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHH
WAAQLYPTARSYLDVYEGFGQLGPRTVFAHCIWLDDADRARMAATGAAISFCPTSNLFLG
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCHHHHHCCCEEEECCCCCEEEC
SGLFDLDRAHAQGVRVGIGTDVGGGTSFSMLQTLNEAYKVLQLNGQHLSPERAFYLATLG
CCCHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHEEEECCCCCCCCCEEEEEEEC
GARSLYLDHRIGNFAPGKEADFVVLDPAATPLLARRALAGTLAERLFVLMMLGDDRCIAA
CCEEEEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHEEEEECCCCEEEE
THILGEPAWTR
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 10913105 [H]