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The map label for this gene is ycdT [H]

Identifier: 119898419

GI number: 119898419

Start: 2333487

End: 2334902

Strand: Direct

Name: ycdT [H]

Synonym: azo2128

Alternate gene names: 119898419

Gene position: 2333487-2334902 (Clockwise)

Preceding gene: 119898416

Following gene: 119898421

Centisome position: 53.32

GC content: 68.29

Gene sequence:

>1416_bases
ATGTCCATTTCGCCGCATCCCTACCCCAACCCGGCCTCGGCGCTGACGCTGCGCCTGACCCGCCTGTACGAACGCGGCCT
GCTGCTCGCCTTTTCACTCAGCCCGCTCGTCGCGATCCTCTACCTGCACCATTTCCAGGCTGACGTGCCCCGCTTCGAAC
ACCATGTGGCGCATGAGCTGGCCATTGCACTGGCGATCGGCGTATCCGCCTTCGTCTGCTACGTCACGCTGCGCTGCTAC
GAGTCGTCCGGCGAAGCCTTCCTGCGCTGGCTGACACTGGGGCTGATCGGTTTTACCGTGGTCTATGCGCCCCATGGCGT
TCTGACCCGCCTGTCGGCCCACCATCTCGACTGGTTCCTGCTCTACGGCCCGGCCTCGCGCTTTGTCATGGCGGCATGCC
TGCTGCGGGCGGCGATGCTGTACGGCGAGGCGCCCGAACCTCGCTGCGCCCGCCCTCGCGCGCGACAGTGGGCGGGCTGG
ATCGGGCTCTTCCTAGGCGTCGACCTCGTGGTCGCGCAACTTTCCTTCCACCCGCACCTGCTGCCCCTTCCCGTCCGACC
GGTATTCGAAGCCTGCGCCATCCTGTTTGCGCTGGCGGCGGCCGGGCTGTTGCTGCGCAAGGGCGCCAGCGCGCCACTGA
TCCTCGTGACCGCGATCGCGCTCGGCATGTTCGCCCAGTCCTCCGTGGCCTTCATGCTGTCCTCCGCCTGGGACCACATC
TGGTGGCTGGCGCATGCGGTGTTCGCCTCGGGTTTCCTGCTGCTGAGCTTCGGCGTCGTGCGCGCCTTTCACACGACCCG
CGCGTTCTCGACCGTTTTCAGCCAGGAAGAAATGATGCGGCAGTTGTACGTGGAGAAGGAGCGGGCGGAATCGGCGCTGG
CGCTGCTGCGCCAGGCCAACGCCGACCTCAGGCGACTGGCGGCCACCGACCCGCTGACCGGCGCCAGCAACCGTCGGCAG
TTCATGGCCACATTGGCGAGCGAATGGGCACGGGCGCGCCGCGAGCGCGCGCCGCTGGCACTGCTTGCGCTCGATATCGA
CCATTTCAAGCACATCAACGACCGTCACGGACACCCCGCCGGCGACCATGCCTTGAAGACCTTCGTGTCCGCCGCGGCGG
GTCTGCTACGCCCAGGCGACGTGATCGGCCGGATCGGCGGCGAGGAGTTCGCCATCGTGCTGCCGGGCACTCAGGCGGCG
GATGCGCACGGCATTGCGGAACGCATCCGGCACGGCGTCGAAGCGCTGGCGCTGGACATCGACGGTGTGCCGGTGGCACT
CACCGTCAGCGTCGGCGTTGCGGTCTCCGGGCCTAGCTGCACCGAGCCCGGGGATCTGCTGAAAGAGGCGGACCGTCGCC
TGTACGAGGCCAAGAACGCCGGCCGCAACCGGGTGTGCCCCCCGCCCCCGGCCTGA

Upstream 100 bases:

>100_bases
GAAGGTTCTGCAAGGGCAGCGCTAGGATCGTCGGAAAAGAACGACGGTCGGGGCCGCTTCAGGTGCCGGCGGTGTCCATA
ATCGAAACGCGAACCGACCA

Downstream 100 bases:

>100_bases
AACCTCAGCGCCAGCGCAATCCGGCGCGCGGAGGCGCCTCGTCGGTCACGCCGCGGCGCAAGTTCTCCAGCAGCCGCCTC
AGCGCTTCGGCGTCCTCTTC

Product: GGDEF-domain-containing protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MSISPHPYPNPASALTLRLTRLYERGLLLAFSLSPLVAILYLHHFQADVPRFEHHVAHELAIALAIGVSAFVCYVTLRCY
ESSGEAFLRWLTLGLIGFTVVYAPHGVLTRLSAHHLDWFLLYGPASRFVMAACLLRAAMLYGEAPEPRCARPRARQWAGW
IGLFLGVDLVVAQLSFHPHLLPLPVRPVFEACAILFALAAAGLLLRKGASAPLILVTAIALGMFAQSSVAFMLSSAWDHI
WWLAHAVFASGFLLLSFGVVRAFHTTRAFSTVFSQEEMMRQLYVEKERAESALALLRQANADLRRLAATDPLTGASNRRQ
FMATLASEWARARRERAPLALLALDIDHFKHINDRHGHPAGDHALKTFVSAAAGLLRPGDVIGRIGGEEFAIVLPGTQAA
DAHGIAERIRHGVEALALDIDGVPVALTVSVGVAVSGPSCTEPGDLLKEADRRLYEAKNAGRNRVCPPPPA

Sequences:

>Translated_471_residues
MSISPHPYPNPASALTLRLTRLYERGLLLAFSLSPLVAILYLHHFQADVPRFEHHVAHELAIALAIGVSAFVCYVTLRCY
ESSGEAFLRWLTLGLIGFTVVYAPHGVLTRLSAHHLDWFLLYGPASRFVMAACLLRAAMLYGEAPEPRCARPRARQWAGW
IGLFLGVDLVVAQLSFHPHLLPLPVRPVFEACAILFALAAAGLLLRKGASAPLILVTAIALGMFAQSSVAFMLSSAWDHI
WWLAHAVFASGFLLLSFGVVRAFHTTRAFSTVFSQEEMMRQLYVEKERAESALALLRQANADLRRLAATDPLTGASNRRQ
FMATLASEWARARRERAPLALLALDIDHFKHINDRHGHPAGDHALKTFVSAAAGLLRPGDVIGRIGGEEFAIVLPGTQAA
DAHGIAERIRHGVEALALDIDGVPVALTVSVGVAVSGPSCTEPGDLLKEADRRLYEAKNAGRNRVCPPPPA
>Mature_470_residues
SISPHPYPNPASALTLRLTRLYERGLLLAFSLSPLVAILYLHHFQADVPRFEHHVAHELAIALAIGVSAFVCYVTLRCYE
SSGEAFLRWLTLGLIGFTVVYAPHGVLTRLSAHHLDWFLLYGPASRFVMAACLLRAAMLYGEAPEPRCARPRARQWAGWI
GLFLGVDLVVAQLSFHPHLLPLPVRPVFEACAILFALAAAGLLLRKGASAPLILVTAIALGMFAQSSVAFMLSSAWDHIW
WLAHAVFASGFLLLSFGVVRAFHTTRAFSTVFSQEEMMRQLYVEKERAESALALLRQANADLRRLAATDPLTGASNRRQF
MATLASEWARARRERAPLALLALDIDHFKHINDRHGHPAGDHALKTFVSAAAGLLRPGDVIGRIGGEEFAIVLPGTQAAD
AHGIAERIRHGVEALALDIDGVPVALTVSVGVAVSGPSCTEPGDLLKEADRRLYEAKNAGRNRVCPPPPA

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=164, Percent_Identity=39.6341463414634, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1786584, Length=163, Percent_Identity=41.7177914110429, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI87082007, Length=144, Percent_Identity=45.8333333333333, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1788085, Length=163, Percent_Identity=34.3558282208589, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI145693134, Length=176, Percent_Identity=38.6363636363636, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI87081881, Length=169, Percent_Identity=38.4615384615385, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787802, Length=159, Percent_Identity=35.8490566037736, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081977, Length=179, Percent_Identity=35.195530726257, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1788381, Length=171, Percent_Identity=32.7485380116959, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1787541, Length=174, Percent_Identity=31.0344827586207, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1787816, Length=159, Percent_Identity=35.2201257861635, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788956, Length=168, Percent_Identity=35.1190476190476, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 51269; Mature: 51138

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISPHPYPNPASALTLRLTRLYERGLLLAFSLSPLVAILYLHHFQADVPRFEHHVAHEL
CCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AIALAIGVSAFVCYVTLRCYESSGEAFLRWLTLGLIGFTVVYAPHGVLTRLSAHHLDWFL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEE
LYGPASRFVMAACLLRAAMLYGEAPEPRCARPRARQWAGWIGLFLGVDLVVAQLSFHPHL
EECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LPLPVRPVFEACAILFALAAAGLLLRKGASAPLILVTAIALGMFAQSSVAFMLSSAWDHI
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WWLAHAVFASGFLLLSFGVVRAFHTTRAFSTVFSQEEMMRQLYVEKERAESALALLRQAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ADLRRLAATDPLTGASNRRQFMATLASEWARARRERAPLALLALDIDHFKHINDRHGHPA
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHCCCCCCCCCC
GDHALKTFVSAAAGLLRPGDVIGRIGGEEFAIVLPGTQAADAHGIAERIRHGVEALALDI
HHHHHHHHHHHHHHCCCCCHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHEEEEC
DGVPVALTVSVGVAVSGPSCTEPGDLLKEADRRLYEAKNAGRNRVCPPPPA
CCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SISPHPYPNPASALTLRLTRLYERGLLLAFSLSPLVAILYLHHFQADVPRFEHHVAHEL
CCCCCCCCCCHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AIALAIGVSAFVCYVTLRCYESSGEAFLRWLTLGLIGFTVVYAPHGVLTRLSAHHLDWFL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEE
LYGPASRFVMAACLLRAAMLYGEAPEPRCARPRARQWAGWIGLFLGVDLVVAQLSFHPHL
EECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LPLPVRPVFEACAILFALAAAGLLLRKGASAPLILVTAIALGMFAQSSVAFMLSSAWDHI
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WWLAHAVFASGFLLLSFGVVRAFHTTRAFSTVFSQEEMMRQLYVEKERAESALALLRQAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ADLRRLAATDPLTGASNRRQFMATLASEWARARRERAPLALLALDIDHFKHINDRHGHPA
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHCCCCCCCCCC
GDHALKTFVSAAAGLLRPGDVIGRIGGEEFAIVLPGTQAADAHGIAERIRHGVEALALDI
HHHHHHHHHHHHHHCCCCCHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHEEEEC
DGVPVALTVSVGVAVSGPSCTEPGDLLKEADRRLYEAKNAGRNRVCPPPPA
CCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]