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The map label for this gene is mhpB

Identifier: 119898266

GI number: 119898266

Start: 2171448

End: 2172401

Strand: Reverse

Name: mhpB

Synonym: azo1975

Alternate gene names: 119898266

Gene position: 2172401-2171448 (Counterclockwise)

Preceding gene: 119898267

Following gene: 119898265

Centisome position: 49.64

GC content: 71.49

Gene sequence:

>954_bases
ATGAGCGCGATGCTGCAATGCATGTCGCATACCCCGCTGAAGGGCTATTTCGACCCGGCGGCGGACGTGGTGGAGGAGGT
GGCCGCGCTGGCCGCCGCGGTGCGCGAGGAGGTGGCCCACTTCGATCCCGAGGTGGTCTATCTCTTCGCCCCGGACCACT
ACAACGGCTTTTTCCTCGACCTGATGCCGCAGTTCTGCCTCGGCATCGAGGCCACATCGGTGGGGGACTACCAGACTCCG
AAAGGGCCGTTGCGGGTGCCGCGCGCGCTGGCCGAGGCCTGCGCCGGCGCGGTGATCGCGCGCGGCATCGACCTCGCATT
CTCCTACCGCATGCAGGTGGACCACGGCTTTGCGCAGACGCTGGTGGAAGTGCTGGGCGGGCTCGCCCGCTACCCGGTGG
TGCCGATCATGATCAACGCGGTGGCACCGCCGCTGGCGAGTTTCCAGCGCGCTCGCCTGCTGGGCGCGGCGGTGGGTGAG
TTTGCCCGCGGGCAGGGCGGGCGCGCGCTCTTCATCGCTTCCGGCGGCATGTCGCACAACCCGCCCATCCCGCAGATCGC
CACCGCCACCGACCCCGAGGTGATCGAGCGCATCGTCGCCGGACGCAACCCCACGCCGGAGGCGCGCGCGGCCCGCCAGC
AGCGCACGGTTGCCGCGGCGCAGGCGTTCGCCGCGGGCGACAGTCCCTTGCGGCCGCTCAATCCGGACTGGGACCGCGCG
CTGATGAAGCGGCTGTGCAGCCGCGACTGGGCGGCGATCGACGCCTACCGCAACGACGAAGTCAGCGCCGCGGCCGGTGC
TTCCGCCCACGAGCTGAAGAGCTGGGTGGCCGCCACGGCGGCGATGGAATCCGCGACCGCCGGCCAGTGGGATGCGCGCG
AGCGCTACTACCGCGCCATCCCCGAATGGCTGGCGGGCTTCGGCGCCATGTCGGGCCTTGGCCGCCACCCCTGA

Upstream 100 bases:

>100_bases
CCAGGTTTTCAATCGCATCCATCTCGCCTTTCTGCTCGGCGGCCACGTGCCCGAAGCCGTCGCGGCGGGCGGCTGATTGC
GCCGCGGAGGAGACACCGGA

Downstream 100 bases:

>100_bases
TCGAACACACACATCACACACGGAGAGGACGTTCAGATGCCCCTCGAATCACCCGAAATCGCCGCAGCAGCAGAACTGCT
GCACCGGGCCGCCAGCCAGC

Product: 3-(2,3-dihydroxyphenyl)propionate dioxygenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MSAMLQCMSHTPLKGYFDPAADVVEEVAALAAAVREEVAHFDPEVVYLFAPDHYNGFFLDLMPQFCLGIEATSVGDYQTP
KGPLRVPRALAEACAGAVIARGIDLAFSYRMQVDHGFAQTLVEVLGGLARYPVVPIMINAVAPPLASFQRARLLGAAVGE
FARGQGGRALFIASGGMSHNPPIPQIATATDPEVIERIVAGRNPTPEARAARQQRTVAAAQAFAAGDSPLRPLNPDWDRA
LMKRLCSRDWAAIDAYRNDEVSAAAGASAHELKSWVAATAAMESATAGQWDARERYYRAIPEWLAGFGAMSGLGRHP

Sequences:

>Translated_317_residues
MSAMLQCMSHTPLKGYFDPAADVVEEVAALAAAVREEVAHFDPEVVYLFAPDHYNGFFLDLMPQFCLGIEATSVGDYQTP
KGPLRVPRALAEACAGAVIARGIDLAFSYRMQVDHGFAQTLVEVLGGLARYPVVPIMINAVAPPLASFQRARLLGAAVGE
FARGQGGRALFIASGGMSHNPPIPQIATATDPEVIERIVAGRNPTPEARAARQQRTVAAAQAFAAGDSPLRPLNPDWDRA
LMKRLCSRDWAAIDAYRNDEVSAAAGASAHELKSWVAATAAMESATAGQWDARERYYRAIPEWLAGFGAMSGLGRHP
>Mature_316_residues
SAMLQCMSHTPLKGYFDPAADVVEEVAALAAAVREEVAHFDPEVVYLFAPDHYNGFFLDLMPQFCLGIEATSVGDYQTPK
GPLRVPRALAEACAGAVIARGIDLAFSYRMQVDHGFAQTLVEVLGGLARYPVVPIMINAVAPPLASFQRARLLGAAVGEF
ARGQGGRALFIASGGMSHNPPIPQIATATDPEVIERIVAGRNPTPEARAARQQRTVAAAQAFAAGDSPLRPLNPDWDRAL
MKRLCSRDWAAIDAYRNDEVSAAAGASAHELKSWVAATAAMESATAGQWDARERYYRAIPEWLAGFGAMSGLGRHP

Specific function: Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3- dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2- hydroxy-6-ketononatrienedioate, respectively

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ligB/mhpB extradiol dioxygenase family

Homologues:

Organism=Escherichia coli, GI1786544, Length=312, Percent_Identity=52.2435897435897, Blast_Score=329, Evalue=2e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MHPB_AZOSB (A1K6Y7)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_933479.1
- ProteinModelPortal:   A1K6Y7
- SMR:   A1K6Y7
- STRING:   A1K6Y7
- GeneID:   4609220
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo1975
- eggNOG:   NOG42398
- HOGENOM:   HBG565586
- OMA:   INWIELM
- ProtClustDB:   PRK13370
- BioCyc:   ASP62928:AZO1975-MONOMER
- HAMAP:   MF_01653
- InterPro:   IPR004183

Pfam domain/function: PF02900 LigB; SSF53213 Xdiol_dOase_3B

EC number: =1.13.11.16

Molecular weight: Translated: 33945; Mature: 33814

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: ACT_SITE 115-115 ACT_SITE 179-179

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAMLQCMSHTPLKGYFDPAADVVEEVAALAAAVREEVAHFDPEVVYLFAPDHYNGFFLD
CHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHH
LMPQFCLGIEATSVGDYQTPKGPLRVPRALAEACAGAVIARGIDLAFSYRMQVDHGFAQT
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVEVLGGLARYPVVPIMINAVAPPLASFQRARLLGAAVGEFARGQGGRALFIASGGMSHN
HHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC
PPIPQIATATDPEVIERIVAGRNPTPEARAARQQRTVAAAQAFAAGDSPLRPLNPDWDRA
CCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
LMKRLCSRDWAAIDAYRNDEVSAAAGASAHELKSWVAATAAMESATAGQWDARERYYRAI
HHHHHHCCCHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
PEWLAGFGAMSGLGRHP
HHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SAMLQCMSHTPLKGYFDPAADVVEEVAALAAAVREEVAHFDPEVVYLFAPDHYNGFFLD
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHH
LMPQFCLGIEATSVGDYQTPKGPLRVPRALAEACAGAVIARGIDLAFSYRMQVDHGFAQT
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVEVLGGLARYPVVPIMINAVAPPLASFQRARLLGAAVGEFARGQGGRALFIASGGMSHN
HHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC
PPIPQIATATDPEVIERIVAGRNPTPEARAARQQRTVAAAQAFAAGDSPLRPLNPDWDRA
CCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
LMKRLCSRDWAAIDAYRNDEVSAAAGASAHELKSWVAATAAMESATAGQWDARERYYRAI
HHHHHHCCCHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
PEWLAGFGAMSGLGRHP
HHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA