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The map label for this gene is thcR [H]

Identifier: 119898256

GI number: 119898256

Start: 2162520

End: 2163521

Strand: Reverse

Name: thcR [H]

Synonym: azo1965

Alternate gene names: 119898256

Gene position: 2163521-2162520 (Counterclockwise)

Preceding gene: 119898257

Following gene: 119898255

Centisome position: 49.44

GC content: 66.07

Gene sequence:

>1002_bases
GTGGATTTGCATGGATGGGATGTCGGCCGCACCGACCACGCCGCGATGGCCAGCTTCGAACGCTTCCATACCCGCGACAT
CGAGGAGGCAAGGCGCTGGGGCACCCGCGTCTTCTGTGAAAACCAGCTGCGCAGCCTGGACGCGCGCAAGCCGGTCGATG
CGCGGATGTACTACCGCAAGCTCAAGGGCATCGGCATTGGCCGCATGAGCTACGGCGGCGAGGTGACCATCGACCCCGGC
CAGCTCGACACCTTCTATCTGGTGCAGATGCCGCTGTGCGGTGGCGAAAGGGTGGAGAGCGCCGGCGCGGTGACGATGTC
CTCGCCCAACACCGGCACCGTGCTCAACGCCCACTGTCCGATCCGCATCCGCCACGGCGAGCGCACCGAGAAGCTAATCG
TGCGGGTAGACCGCGACCTGCTCGAACGCCAGTGCAGCCAGCACCTCGGCCGCACGCTGCAGCGGCACATCACTTTCGAC
ACCGCGATGCCGCTGGATACCGGCGGCGGCATCCGCTGGATGCGCACGATCGCGTGGCTGCTGGAGAACCTGCGCGACGA
GGACGGCCCCGCGTCGCCGCTGCTGTCGGCCCAGATCGAGCAGATGCTCGTGACGATGCTGCTGGTGTGCCAGCCCAACA
ATTATTCCGGCGCACTCCATGAAGACGTCCGCACCGTCACCCCGGCTTTCGTGCGGCGCGCGGAACACTACATCGACGAG
CACGCGCACGAAGCGATCACCGTGTGCGATATCGCCGAGCACGTCGGCGTCAGCAGCCGCTCGCTGTACACCGGCTTCCG
CAAATACCGCAACACCTCGCCCATGCTGTACCTGAAGGAAACCCGCCTGAACCGGGTGCGCGAACAGTTGCTGAGCACCG
AACCGGGGCGCACCACGGTGACTGCGGTGGCCTACCAGTGGGGATTCGGCCACCTCGGCCATTTCACCACCGACTACAAG
CGCCGCTTCGGAGAAAGCCCCTCCGAAACCCTGGCCCGCTAG

Upstream 100 bases:

>100_bases
GCCGGCCCGGGGCCGGCTTGCAGATCGCGCCTCCGGCATCGCCAGACCCGCAACGAGCGGGCAGGGGGCGCGCCCCTCAG
AGAGACACGAGGAGTCAGAC

Downstream 100 bases:

>100_bases
CGCCCCTTGCCGGCCGGCACGAACACTGCTTGGATGCGGGCCGACGGCAACGCAGGAACCGGGGGAGGGCGGAGGCTCAG
GACGATGGACAAGGGTATGC

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MDLHGWDVGRTDHAAMASFERFHTRDIEEARRWGTRVFCENQLRSLDARKPVDARMYYRKLKGIGIGRMSYGGEVTIDPG
QLDTFYLVQMPLCGGERVESAGAVTMSSPNTGTVLNAHCPIRIRHGERTEKLIVRVDRDLLERQCSQHLGRTLQRHITFD
TAMPLDTGGGIRWMRTIAWLLENLRDEDGPASPLLSAQIEQMLVTMLLVCQPNNYSGALHEDVRTVTPAFVRRAEHYIDE
HAHEAITVCDIAEHVGVSSRSLYTGFRKYRNTSPMLYLKETRLNRVREQLLSTEPGRTTVTAVAYQWGFGHLGHFTTDYK
RRFGESPSETLAR

Sequences:

>Translated_333_residues
MDLHGWDVGRTDHAAMASFERFHTRDIEEARRWGTRVFCENQLRSLDARKPVDARMYYRKLKGIGIGRMSYGGEVTIDPG
QLDTFYLVQMPLCGGERVESAGAVTMSSPNTGTVLNAHCPIRIRHGERTEKLIVRVDRDLLERQCSQHLGRTLQRHITFD
TAMPLDTGGGIRWMRTIAWLLENLRDEDGPASPLLSAQIEQMLVTMLLVCQPNNYSGALHEDVRTVTPAFVRRAEHYIDE
HAHEAITVCDIAEHVGVSSRSLYTGFRKYRNTSPMLYLKETRLNRVREQLLSTEPGRTTVTAVAYQWGFGHLGHFTTDYK
RRFGESPSETLAR
>Mature_333_residues
MDLHGWDVGRTDHAAMASFERFHTRDIEEARRWGTRVFCENQLRSLDARKPVDARMYYRKLKGIGIGRMSYGGEVTIDPG
QLDTFYLVQMPLCGGERVESAGAVTMSSPNTGTVLNAHCPIRIRHGERTEKLIVRVDRDLLERQCSQHLGRTLQRHITFD
TAMPLDTGGGIRWMRTIAWLLENLRDEDGPASPLLSAQIEQMLVTMLLVCQPNNYSGALHEDVRTVTPAFVRRAEHYIDE
HAHEAITVCDIAEHVGVSSRSLYTGFRKYRNTSPMLYLKETRLNRVREQLLSTEPGRTTVTAVAYQWGFGHLGHFTTDYK
RRFGESPSETLAR

Specific function: Probably involved in the positive regulation of the thc operon for the degradation of the thiocarbamate herbicide EPTC [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788778, Length=102, Percent_Identity=42.156862745098, Blast_Score=85, Evalue=5e-18,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 37934; Mature: 37934

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLHGWDVGRTDHAAMASFERFHTRDIEEARRWGTRVFCENQLRSLDARKPVDARMYYRK
CCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCHHEEHHHHHHCCCCCCHHHHHHHHH
LKGIGIGRMSYGGEVTIDPGQLDTFYLVQMPLCGGERVESAGAVTMSSPNTGTVLNAHCP
HHCCCCEEECCCCEEEECCCCCCEEEEEEECCCCCCHHHCCCEEEECCCCCCCEEECCCC
IRIRHGERTEKLIVRVDRDLLERQCSQHLGRTLQRHITFDTAMPLDTGGGIRWMRTIAWL
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
LENLRDEDGPASPLLSAQIEQMLVTMLLVCQPNNYSGALHEDVRTVTPAFVRRAEHYIDE
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
HAHEAITVCDIAEHVGVSSRSLYTGFRKYRNTSPMLYLKETRLNRVREQLLSTEPGRTTV
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCCCEEE
TAVAYQWGFGHLGHFTTDYKRRFGESPSETLAR
EEEEEECCCCCCCCCHHHHHHHCCCCHHHHHCC
>Mature Secondary Structure
MDLHGWDVGRTDHAAMASFERFHTRDIEEARRWGTRVFCENQLRSLDARKPVDARMYYRK
CCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCHHEEHHHHHHCCCCCCHHHHHHHHH
LKGIGIGRMSYGGEVTIDPGQLDTFYLVQMPLCGGERVESAGAVTMSSPNTGTVLNAHCP
HHCCCCEEECCCCEEEECCCCCCEEEEEEECCCCCCHHHCCCEEEECCCCCCCEEECCCC
IRIRHGERTEKLIVRVDRDLLERQCSQHLGRTLQRHITFDTAMPLDTGGGIRWMRTIAWL
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
LENLRDEDGPASPLLSAQIEQMLVTMLLVCQPNNYSGALHEDVRTVTPAFVRRAEHYIDE
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
HAHEAITVCDIAEHVGVSSRSLYTGFRKYRNTSPMLYLKETRLNRVREQLLSTEPGRTTV
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCCCEEE
TAVAYQWGFGHLGHFTTDYKRRFGESPSETLAR
EEEEEECCCCCCCCCHHHHHHHCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]