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The map label for this gene is thcR [H]

Identifier: 119898227

GI number: 119898227

Start: 2125695

End: 2126702

Strand: Direct

Name: thcR [H]

Synonym: azo1936

Alternate gene names: 119898227

Gene position: 2125695-2126702 (Clockwise)

Preceding gene: 119898217

Following gene: 119898228

Centisome position: 48.58

GC content: 68.85

Gene sequence:

>1008_bases
TTGAACGCGCCTGACGCCGGGCTGGCGGAGCCGCTGGCCGCCTATCCCTTCTTCCGCTCCAGTGATGCGGAGGAATCGCG
CCAGCACGTCGCCCGCGTATTCTGCCCGCACGAACTTCGCCAAGTCACGGGCCGGCGGCCGTTCGCCTCGCGCCACAACA
TGCTGCGGCTGCACGAGACGGCGCTGAACTACCTGACCTACGGCACCGACGTCGACATCCTTCCCGGCCGGCTCGAAAGC
TTCTATCTGGTCCAGATGCCCCTGGCGGGCGGCGCCGATATCCGCTGCGGCGAGCAGGCGGTGCTGTCCTCTCCCCGGCT
GGCCACCATCCTCAGCCCGGACGATGAAACCCGGATGCGCTGGCAGCACGATGCGCGCCAGCTGCTGCTGTGGATTCCGC
GCGAGGCCTTGGAGCGGCGGCTGGAAGAGCATCTGGGCGAACGCCCGGACCGCCCGCTCTCCTTCCACCTCGGCCTGCCG
CAGGACGAAGGGCTGACCCGCAGCTGGTGCCGGATGCTCGCTGACCTTGCGGCCAACATCGACCAGTGCGGGGCCGACTG
GCTCCGCTTCCGCCCCACCGTCGCCGCCCTGGAGGATTGTCTGTTGCGCGGATTACTGCAGCTTCACCAGCACAACTACA
GCGACTGCCTGAACGCACCGCGCGACACCGGCAAGCCGCGCCACGTCCAGCGCGCGATGGACTACGTGCAGGCGCACATT
GAAGAGGGCATCAGCGTGGGCGACATCGCACGCGCCGCCTGTGTCAGCGTGCGGGCGCTCGAAGAAGGCTTCCGCAAGTA
TTGCGACACCACGCCGCTCGCCTATCTGCGCGAACTGCGGCTGGAGCGCGCGCGCAAGGCCCTGCTGGCCCTGCCCGGCC
GCAGCGAATCGGTGACCGACGTCGCGCATCGCTACGGCTTCGTCCATCTCGGGCGCTTCTCCGCGTATTACAAGGCGCGC
TTCGGCGAAACGCCCTCGCAGACGGTCGGTCGCGCGCGGCGCTGCTGA

Upstream 100 bases:

>100_bases
TGTTGCATTTAAATTATTTAGGTCTATACATTATGGGCGAGGGTGCACGCCATGTCAGTTCTCCCGTTGCTGCCGACCGG
CCCGCTGCCGGCCTACCGCC

Downstream 100 bases:

>100_bases
TCCCTGTCGGACAGCGCATAACGGATAGGTCCAGAACGGATTCCGGATAGCCCGTCACCGGGCTGAAATGAAAACTGTGC
TCCATCGACGCCGCTGCGCG

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MNAPDAGLAEPLAAYPFFRSSDAEESRQHVARVFCPHELRQVTGRRPFASRHNMLRLHETALNYLTYGTDVDILPGRLES
FYLVQMPLAGGADIRCGEQAVLSSPRLATILSPDDETRMRWQHDARQLLLWIPREALERRLEEHLGERPDRPLSFHLGLP
QDEGLTRSWCRMLADLAANIDQCGADWLRFRPTVAALEDCLLRGLLQLHQHNYSDCLNAPRDTGKPRHVQRAMDYVQAHI
EEGISVGDIARAACVSVRALEEGFRKYCDTTPLAYLRELRLERARKALLALPGRSESVTDVAHRYGFVHLGRFSAYYKAR
FGETPSQTVGRARRC

Sequences:

>Translated_335_residues
MNAPDAGLAEPLAAYPFFRSSDAEESRQHVARVFCPHELRQVTGRRPFASRHNMLRLHETALNYLTYGTDVDILPGRLES
FYLVQMPLAGGADIRCGEQAVLSSPRLATILSPDDETRMRWQHDARQLLLWIPREALERRLEEHLGERPDRPLSFHLGLP
QDEGLTRSWCRMLADLAANIDQCGADWLRFRPTVAALEDCLLRGLLQLHQHNYSDCLNAPRDTGKPRHVQRAMDYVQAHI
EEGISVGDIARAACVSVRALEEGFRKYCDTTPLAYLRELRLERARKALLALPGRSESVTDVAHRYGFVHLGRFSAYYKAR
FGETPSQTVGRARRC
>Mature_335_residues
MNAPDAGLAEPLAAYPFFRSSDAEESRQHVARVFCPHELRQVTGRRPFASRHNMLRLHETALNYLTYGTDVDILPGRLES
FYLVQMPLAGGADIRCGEQAVLSSPRLATILSPDDETRMRWQHDARQLLLWIPREALERRLEEHLGERPDRPLSFHLGLP
QDEGLTRSWCRMLADLAANIDQCGADWLRFRPTVAALEDCLLRGLLQLHQHNYSDCLNAPRDTGKPRHVQRAMDYVQAHI
EEGISVGDIARAACVSVRALEEGFRKYCDTTPLAYLRELRLERARKALLALPGRSESVTDVAHRYGFVHLGRFSAYYKAR
FGETPSQTVGRARRC

Specific function: Probably involved in the positive regulation of the thc operon for the degradation of the thiocarbamate herbicide EPTC [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788778, Length=108, Percent_Identity=34.2592592592593, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 38069; Mature: 38069

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPDAGLAEPLAAYPFFRSSDAEESRQHVARVFCPHELRQVTGRRPFASRHNMLRLHET
CCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHH
ALNYLTYGTDVDILPGRLESFYLVQMPLAGGADIRCGEQAVLSSPRLATILSPDDETRMR
HHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHCCCCEEEEECCCHHHHHH
WQHDARQLLLWIPREALERRLEEHLGERPDRPLSFHLGLPQDEGLTRSWCRMLADLAANI
HHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCH
DQCGADWLRFRPTVAALEDCLLRGLLQLHQHNYSDCLNAPRDTGKPRHVQRAMDYVQAHI
HHHCHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHHH
EEGISVGDIARAACVSVRALEEGFRKYCDTTPLAYLRELRLERARKALLALPGRSESVTD
HCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
VAHRYGFVHLGRFSAYYKARFGETPSQTVGRARRC
HHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHCCC
>Mature Secondary Structure
MNAPDAGLAEPLAAYPFFRSSDAEESRQHVARVFCPHELRQVTGRRPFASRHNMLRLHET
CCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHH
ALNYLTYGTDVDILPGRLESFYLVQMPLAGGADIRCGEQAVLSSPRLATILSPDDETRMR
HHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHCCCCEEEEECCCHHHHHH
WQHDARQLLLWIPREALERRLEEHLGERPDRPLSFHLGLPQDEGLTRSWCRMLADLAANI
HHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCH
DQCGADWLRFRPTVAALEDCLLRGLLQLHQHNYSDCLNAPRDTGKPRHVQRAMDYVQAHI
HHHCHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHHH
EEGISVGDIARAACVSVRALEEGFRKYCDTTPLAYLRELRLERARKALLALPGRSESVTD
HCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
VAHRYGFVHLGRFSAYYKARFGETPSQTVGRARRC
HHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]