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The map label for this gene is rmlB [H]

Identifier: 119898168

GI number: 119898168

Start: 2059250

End: 2060326

Strand: Reverse

Name: rmlB [H]

Synonym: azo1877

Alternate gene names: 119898168

Gene position: 2060326-2059250 (Counterclockwise)

Preceding gene: 119898171

Following gene: 119898167

Centisome position: 47.08

GC content: 64.25

Gene sequence:

>1077_bases
ATGATTCTCGTCACAGGTGGCGCCGGCTTCATCGGCGGAAATTTCGTGCTCGACTGGATCGCCGAAACCGGCGAGCCGGT
TCTCAACCTCGACGCGCTCACCTATGCCGGCAACCTCGAAACCCTGGCTTCGCTGGCGGACGACGAGCGGCACGTGTTCA
AGCGCGGCGACATCTGCGACCGCGAGTTGGTCGATGGCCTGCTGCAGACCTACCGCCCGCGCGCCATCGTGCACTTTGCC
GCCGAGAGCCATGTCGACCGGTCGATTCACGGTCCCGGGGAATTCATCCGCACCAACGTCCAGGGCACCTTCACCCTGCT
CGAAGCCGCGCGCGCCTACTGGAACGGGCTGCCCCCCGACGCGCGCGCCGGATTCCGTTTCCTGCACGTCAGCACCGACG
AGGTCTACGGATCGCTCGAACCGCAAGCGCCTGCATTCACCGAACGTCATCCCTTCGAACCCAACAGCCCGTATTCCGCT
AGCAAAGCGGCGTCCGACCACCTCGTACGCGCCTGGTATCACACCTACGGGCTTCCGGTGCTCACCACCAACTGCTCCAA
CAACTACGGCCCGTTCCACTTCCCGGAGAAGCTGATCCCCCTGATGATCGTCAACGCGCTCGCCGGGCGGCCGCTGCCGG
TGTATGGCGACGGCCTCAACGTGCGCGACTGGCTGTACGTGAAGGACCACTGCAGCGCCATCCGGCGCGTGCTGGCCCAA
GGGCGGTTGGGTGAAACCTATAACGTCGGCGGCTGGAACGAAAAGCGCAACATCGACATCGTGCATACCGTCTGCGCGCT
GCTCGACGAAATGCGCCCCGACCCCGCGGGTAGCTATGCACGACTGATCACCTTCGTGCAGGACCGTCCCGGCCACGATC
GCCGCTACGCGATCGACGCCAGCAAGATCGAAAACGAACTCGGCTGGCGGCCCGCCGAGACGTTCGAAACGGGTATCCGC
AAGACGATCGCCTGGTACCTCGAAAACCCCGGATGGGTGGAACACGTCCAGAGCGGTGCGTACCGCAACTGGATCGACAC
AAACTACGCGTCGCGGCAAAACGGCGGAACGGCATGA

Upstream 100 bases:

>100_bases
AGAACACCGCCGCTCCCCGAACCCGTCTGTCTGACGGGAGAGTTTCCACGATTCGATATAATCGCCGCTTGCCGCTGCGC
GGCCCCTCTGGACCTCCCAC

Downstream 100 bases:

>100_bases
AGATCCTGCTGTTGGGCAAGAACGGGCAGGTTGGATGGGAACTGCAGCGCGCGCTGGCGCCCTTGGGCGAAGTTCTGGCG
TTGGACCGGCACGGTAGCGA

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MILVTGGAGFIGGNFVLDWIAETGEPVLNLDALTYAGNLETLASLADDERHVFKRGDICDRELVDGLLQTYRPRAIVHFA
AESHVDRSIHGPGEFIRTNVQGTFTLLEAARAYWNGLPPDARAGFRFLHVSTDEVYGSLEPQAPAFTERHPFEPNSPYSA
SKAASDHLVRAWYHTYGLPVLTTNCSNNYGPFHFPEKLIPLMIVNALAGRPLPVYGDGLNVRDWLYVKDHCSAIRRVLAQ
GRLGETYNVGGWNEKRNIDIVHTVCALLDEMRPDPAGSYARLITFVQDRPGHDRRYAIDASKIENELGWRPAETFETGIR
KTIAWYLENPGWVEHVQSGAYRNWIDTNYASRQNGGTA

Sequences:

>Translated_358_residues
MILVTGGAGFIGGNFVLDWIAETGEPVLNLDALTYAGNLETLASLADDERHVFKRGDICDRELVDGLLQTYRPRAIVHFA
AESHVDRSIHGPGEFIRTNVQGTFTLLEAARAYWNGLPPDARAGFRFLHVSTDEVYGSLEPQAPAFTERHPFEPNSPYSA
SKAASDHLVRAWYHTYGLPVLTTNCSNNYGPFHFPEKLIPLMIVNALAGRPLPVYGDGLNVRDWLYVKDHCSAIRRVLAQ
GRLGETYNVGGWNEKRNIDIVHTVCALLDEMRPDPAGSYARLITFVQDRPGHDRRYAIDASKIENELGWRPAETFETGIR
KTIAWYLENPGWVEHVQSGAYRNWIDTNYASRQNGGTA
>Mature_358_residues
MILVTGGAGFIGGNFVLDWIAETGEPVLNLDALTYAGNLETLASLADDERHVFKRGDICDRELVDGLLQTYRPRAIVHFA
AESHVDRSIHGPGEFIRTNVQGTFTLLEAARAYWNGLPPDARAGFRFLHVSTDEVYGSLEPQAPAFTERHPFEPNSPYSA
SKAASDHLVRAWYHTYGLPVLTTNCSNNYGPFHFPEKLIPLMIVNALAGRPLPVYGDGLNVRDWLYVKDHCSAIRRVLAQ
GRLGETYNVGGWNEKRNIDIVHTVCALLDEMRPDPAGSYARLITFVQDRPGHDRRYAIDASKIENELGWRPAETFETGIR
KTIAWYLENPGWVEHVQSGAYRNWIDTNYASRQNGGTA

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=330, Percent_Identity=37.5757575757576, Blast_Score=223, Evalue=1e-58,
Organism=Homo sapiens, GI42516563, Length=330, Percent_Identity=25.7575757575758, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI56237023, Length=359, Percent_Identity=27.2980501392758, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI56118217, Length=359, Percent_Identity=27.2980501392758, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI189083684, Length=359, Percent_Identity=27.2980501392758, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1788353, Length=360, Percent_Identity=59.4444444444444, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI48994969, Length=344, Percent_Identity=61.3372093023256, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI1786974, Length=356, Percent_Identity=27.5280898876405, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788366, Length=359, Percent_Identity=24.5125348189415, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17568069, Length=335, Percent_Identity=37.0149253731343, Blast_Score=201, Evalue=5e-52,
Organism=Caenorhabditis elegans, GI115532424, Length=334, Percent_Identity=31.1377245508982, Blast_Score=161, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17539532, Length=332, Percent_Identity=24.0963855421687, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6319493, Length=365, Percent_Identity=25.4794520547945, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI19923002, Length=361, Percent_Identity=28.5318559556787, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21356223, Length=330, Percent_Identity=24.5454545454545, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40040; Mature: 40040

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILVTGGAGFIGGNFVLDWIAETGEPVLNLDALTYAGNLETLASLADDERHVFKRGDICD
CEEEECCCCCCCCHHHHHHHHHCCCCEEEEEHEEECCCHHHHHHHHCCHHHHHHCCCCCC
RELVDGLLQTYRPRAIVHFAAESHVDRSIHGPGEFIRTNVQGTFTLLEAARAYWNGLPPD
HHHHHHHHHHCCCCEEEEEHHHHCCCCCCCCCHHHEEECCCHHHHHHHHHHHHHCCCCCC
ARAGFRFLHVSTDEVYGSLEPQAPAFTERHPFEPNSPYSASKAASDHLVRAWYHTYGLPV
CCCCEEEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEE
LTTNCSNNYGPFHFPEKLIPLMIVNALAGRPLPVYGDGLNVRDWLYVKDHCSAIRRVLAQ
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCEEEEHHHHHHHHHHHHC
GRLGETYNVGGWNEKRNIDIVHTVCALLDEMRPDPAGSYARLITFVQDRPGHDRRYAIDA
CCCCCEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEECH
SKIENELGWRPAETFETGIRKTIAWYLENPGWVEHVQSGAYRNWIDTNYASRQNGGTA
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MILVTGGAGFIGGNFVLDWIAETGEPVLNLDALTYAGNLETLASLADDERHVFKRGDICD
CEEEECCCCCCCCHHHHHHHHHCCCCEEEEEHEEECCCHHHHHHHHCCHHHHHHCCCCCC
RELVDGLLQTYRPRAIVHFAAESHVDRSIHGPGEFIRTNVQGTFTLLEAARAYWNGLPPD
HHHHHHHHHHCCCCEEEEEHHHHCCCCCCCCCHHHEEECCCHHHHHHHHHHHHHCCCCCC
ARAGFRFLHVSTDEVYGSLEPQAPAFTERHPFEPNSPYSASKAASDHLVRAWYHTYGLPV
CCCCEEEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEE
LTTNCSNNYGPFHFPEKLIPLMIVNALAGRPLPVYGDGLNVRDWLYVKDHCSAIRRVLAQ
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCEEEEHHHHHHHHHHHHC
GRLGETYNVGGWNEKRNIDIVHTVCALLDEMRPDPAGSYARLITFVQDRPGHDRRYAIDA
CCCCCEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEECH
SKIENELGWRPAETFETGIRKTIAWYLENPGWVEHVQSGAYRNWIDTNYASRQNGGTA
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]