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The map label for this gene is rfbD [H]

Identifier: 119898167

GI number: 119898167

Start: 2058363

End: 2059253

Strand: Reverse

Name: rfbD [H]

Synonym: azo1876

Alternate gene names: 119898167

Gene position: 2059253-2058363 (Counterclockwise)

Preceding gene: 119898168

Following gene: 119898166

Centisome position: 47.06

GC content: 67.79

Gene sequence:

>891_bases
ATGAAGATCCTGCTGTTGGGCAAGAACGGGCAGGTTGGATGGGAACTGCAGCGCGCGCTGGCGCCCTTGGGCGAAGTTCT
GGCGTTGGACCGGCACGGTAGCGACGGTTTGTGTGGCGACCTTTCCCGCCCCGAAGAACTGGGCGACACGGTGCGCGCAG
TAGCGCCGCAGGTCATCGTTATCGCGGCGGCATACACCGCGGTGGACAAGGCCGAGTCGGACGCCGCGCTCGCGCAGCGG
ATCAATGCGGATGCCGTCGCGGAACTCGCGCGCCAGGCGACGCAACTCGGCGCGCTGCTGGTTCATTACTCCACCGATTA
CGTCTTCGATGGCAGCGGCAGCACCCCCTGGCGCGAAGATGCGCCGGTCGCCCCCTTGTCGGTCTACGGCACCACCAAGC
TGGCGGGCGAAACGGCGATTCGCGCCTCGGGCTGCCGCCACCTCATTTTCCGCACGTCCTGGGTGTATGCGGCCCGCGGC
GGCAACTTCGCCAAGACGATGCTGCGGCTTGCGGCCGAACGGGACACCTTGAAGGTGGTCGCGGACCAGATCGGCGCGCC
CACGGGTGCCGATCTCATCGCCGATGTGACCGCCCACGCGATCCGCGCCTGCATCCCGTCCGCCAACGACCTTTCCGGCA
CGTATCACCTCGCCGCGGCGGGCGAGACCAGTTGGCATGGCTACGCCTGCCACGTCATCGACACGGCGCGCCGGTTGGGA
CACCCGCTGAAGGTCGGCCTCATCGAACCGCTCGCAAGCGCAGACTACCCAACCCCCGCCCGCCGGCCGGCCAACTCCCG
GCTCGACACTGCGTTGCTGCGCACGACGTTCGCCCTCAACCTGCCTGACTGGCGCAGCGGGGTCGACCGCATGCTTCAGG
AAACCCTTTAA

Upstream 100 bases:

>100_bases
GGTACCTCGAAAACCCCGGATGGGTGGAACACGTCCAGAGCGGTGCGTACCGCAACTGGATCGACACAAACTACGCGTCG
CGGCAAAACGGCGGAACGGC

Downstream 100 bases:

>100_bases
GGACCACTCAATTGGCACGCCGCAAGGGCATCATCCTCGCCGGAGGCTCCGGCACACGCCTGCATCCCGCAACGCTCGCG
GTTTCAAAGCAGCTGCTGCC

Product: dTDP-4-dehydrorhamnose reductase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MKILLLGKNGQVGWELQRALAPLGEVLALDRHGSDGLCGDLSRPEELGDTVRAVAPQVIVIAAAYTAVDKAESDAALAQR
INADAVAELARQATQLGALLVHYSTDYVFDGSGSTPWREDAPVAPLSVYGTTKLAGETAIRASGCRHLIFRTSWVYAARG
GNFAKTMLRLAAERDTLKVVADQIGAPTGADLIADVTAHAIRACIPSANDLSGTYHLAAAGETSWHGYACHVIDTARRLG
HPLKVGLIEPLASADYPTPARRPANSRLDTALLRTTFALNLPDWRSGVDRMLQETL

Sequences:

>Translated_296_residues
MKILLLGKNGQVGWELQRALAPLGEVLALDRHGSDGLCGDLSRPEELGDTVRAVAPQVIVIAAAYTAVDKAESDAALAQR
INADAVAELARQATQLGALLVHYSTDYVFDGSGSTPWREDAPVAPLSVYGTTKLAGETAIRASGCRHLIFRTSWVYAARG
GNFAKTMLRLAAERDTLKVVADQIGAPTGADLIADVTAHAIRACIPSANDLSGTYHLAAAGETSWHGYACHVIDTARRLG
HPLKVGLIEPLASADYPTPARRPANSRLDTALLRTTFALNLPDWRSGVDRMLQETL
>Mature_296_residues
MKILLLGKNGQVGWELQRALAPLGEVLALDRHGSDGLCGDLSRPEELGDTVRAVAPQVIVIAAAYTAVDKAESDAALAQR
INADAVAELARQATQLGALLVHYSTDYVFDGSGSTPWREDAPVAPLSVYGTTKLAGETAIRASGCRHLIFRTSWVYAARG
GNFAKTMLRLAAERDTLKVVADQIGAPTGADLIADVTAHAIRACIPSANDLSGTYHLAAAGETSWHGYACHVIDTARRLG
HPLKVGLIEPLASADYPTPARRPANSRLDTALLRTTFALNLPDWRSGVDRMLQETL

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=301, Percent_Identity=27.5747508305648, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI11034825, Length=301, Percent_Identity=27.5747508305648, Blast_Score=85, Evalue=9e-17,
Organism=Escherichia coli, GI1788352, Length=294, Percent_Identity=57.8231292517007, Blast_Score=351, Evalue=4e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 31526; Mature: 31526

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILLLGKNGQVGWELQRALAPLGEVLALDRHGSDGLCGDLSRPEELGDTVRAVAPQVIV
CEEEEECCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHCCHHEE
IAAAYTAVDKAESDAALAQRINADAVAELARQATQLGALLVHYSTDYVFDGSGSTPWRED
EEHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHEEEEECCCEEEECCCCCCCCCC
APVAPLSVYGTTKLAGETAIRASGCRHLIFRTSWVYAARGGNFAKTMLRLAAERDTLKVV
CCCCCEEECCCHHHCCCHHHHHCCCCEEEEEEEEEEEECCCHHHHHHHHHHHCCHHHHHH
ADQIGAPTGADLIADVTAHAIRACIPSANDLSGTYHLAAAGETSWHGYACHVIDTARRLG
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCEEEHHHHHHHHCC
HPLKVGLIEPLASADYPTPARRPANSRLDTALLRTTFALNLPDWRSGVDRMLQETL
CCEEEHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHEECCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKILLLGKNGQVGWELQRALAPLGEVLALDRHGSDGLCGDLSRPEELGDTVRAVAPQVIV
CEEEEECCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHCCHHEE
IAAAYTAVDKAESDAALAQRINADAVAELARQATQLGALLVHYSTDYVFDGSGSTPWRED
EEHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHEEEEECCCEEEECCCCCCCCCC
APVAPLSVYGTTKLAGETAIRASGCRHLIFRTSWVYAARGGNFAKTMLRLAAERDTLKVV
CCCCCEEECCCHHHCCCHHHHHCCCCEEEEEEEEEEEECCCHHHHHHHHHHHCCHHHHHH
ADQIGAPTGADLIADVTAHAIRACIPSANDLSGTYHLAAAGETSWHGYACHVIDTARRLG
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCEEEHHHHHHHHCC
HPLKVGLIEPLASADYPTPARRPANSRLDTALLRTTFALNLPDWRSGVDRMLQETL
CCEEEHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHEECCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]