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The map label for this gene is ispF

Identifier: 119897974

GI number: 119897974

Start: 1847624

End: 1848112

Strand: Direct

Name: ispF

Synonym: azo1683

Alternate gene names: 119897974

Gene position: 1847624-1848112 (Clockwise)

Preceding gene: 119897973

Following gene: 119897979

Centisome position: 42.22

GC content: 70.35

Gene sequence:

>489_bases
ATGAAATGTCCGTTCCGCGTCGGCCAGGGCTTCGACGTACACGCGCTGGTGCCGGGCCGGGCGCTGATCGTCGGGGGGGT
CGATATTCCGTTCTCCGCCGGGCTGCTCGGCCATTCGGATGCCGACGTGCTGCTGCATGCGCTGACCGACGCGCTGCTCG
GTGCCGCCGGACTGGGCGACATCGGGCGGCTCTTTCCCGATACCGACCCGGCGCATGCCGGCGCTGACAGCCGGGTGCTG
CTGCGCGAGGCCTACGCGCGGGTGAAGGCGGCCGGCTGGGAAGCGGTGAATGTGGACGCGACGCTGATCTGCCGGGCGCC
CCGCATCCTGCCGCACGCGCCGGCGATGGTCGTCAACATCGCCGCCGACCTCGGCATGGATGCGGCGGCGATCAACATCA
AGGGCAAGACCACCGAAAAGCTCGGCTTCACCGGGCGCGGCGAAGGCATCGCGGCGCAGGTCGTCGCGCTGCTGGCACGC
GCGGATTAA

Upstream 100 bases:

>100_bases
CACGGCTGGTGCAGGGTGACGCGACCAACCTGAAGGTGACCTATCCGCTCGACCTCCACCTGGCCGAGTGGATTCTCAAC
AACCGTACTGGAGTGCAGCA

Downstream 100 bases:

>100_bases
CCGGGCGCGACGCACAAAAGAAGCGGGGCGCCAAGCGCCCCGCGTGGGTCAGGGCCGCCCGGGACGGCCCTTTCGTCTCC
CCGAGGGAGACGCTCCAAGA

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MKCPFRVGQGFDVHALVPGRALIVGGVDIPFSAGLLGHSDADVLLHALTDALLGAAGLGDIGRLFPDTDPAHAGADSRVL
LREAYARVKAAGWEAVNVDATLICRAPRILPHAPAMVVNIAADLGMDAAAINIKGKTTEKLGFTGRGEGIAAQVVALLAR
AD

Sequences:

>Translated_162_residues
MKCPFRVGQGFDVHALVPGRALIVGGVDIPFSAGLLGHSDADVLLHALTDALLGAAGLGDIGRLFPDTDPAHAGADSRVL
LREAYARVKAAGWEAVNVDATLICRAPRILPHAPAMVVNIAADLGMDAAAINIKGKTTEKLGFTGRGEGIAAQVVALLAR
AD
>Mature_162_residues
MKCPFRVGQGFDVHALVPGRALIVGGVDIPFSAGLLGHSDADVLLHALTDALLGAAGLGDIGRLFPDTDPAHAGADSRVL
LREAYARVKAAGWEAVNVDATLICRAPRILPHAPAMVVNIAADLGMDAAAINIKGKTTEKLGFTGRGEGIAAQVVALLAR
AD

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=157, Percent_Identity=63.6942675159236, Blast_Score=190, Evalue=5e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_AZOSB (A1K645)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_933187.1
- ProteinModelPortal:   A1K645
- SMR:   A1K645
- STRING:   A1K645
- GeneID:   4606975
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo1683
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- PhylomeDB:   A1K645
- ProtClustDB:   PRK00084
- BioCyc:   ASP62928:AZO1683-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 16620; Mature: 16620

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKCPFRVGQGFDVHALVPGRALIVGGVDIPFSAGLLGHSDADVLLHALTDALLGAAGLGD
CCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
IGRLFPDTDPAHAGADSRVLLREAYARVKAAGWEAVNVDATLICRAPRILPHAPAMVVNI
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCEEEEEECCCCCCCCCHHEEEE
AADLGMDAAAINIKGKTTEKLGFTGRGEGIAAQVVALLARAD
HHHCCCCEEEEEECCCCCHHCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKCPFRVGQGFDVHALVPGRALIVGGVDIPFSAGLLGHSDADVLLHALTDALLGAAGLGD
CCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
IGRLFPDTDPAHAGADSRVLLREAYARVKAAGWEAVNVDATLICRAPRILPHAPAMVVNI
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCEEEEEECCCCCCCCCHHEEEE
AADLGMDAAAINIKGKTTEKLGFTGRGEGIAAQVVALLARAD
HHHCCCCEEEEEECCCCCHHCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA