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The map label for this gene is ispD

Identifier: 119897973

GI number: 119897973

Start: 1846917

End: 1847627

Strand: Direct

Name: ispD

Synonym: azo1682

Alternate gene names: 119897973

Gene position: 1846917-1847627 (Clockwise)

Preceding gene: 119897962

Following gene: 119897974

Centisome position: 42.21

GC content: 69.48

Gene sequence:

>711_bases
ATGCAGAATTTCCATCCCCGCCATTTCGCCATCGTGCCCGCCGCCGGCAGCGGCAGCCGCATGGGCAGCGAGCGTCCCAA
GCAGTACCTGCCGCTGCTCGGCAAGCCCCTCATCTACCACACGCTGTCGGTGCTGTGCGCCGCGCCTGAGATCGACAAGG
TGTTCGTCGTGCTGTCGGTCGGCGACGCCGAGTGGGGCCGCCACGACTGGCGCGCGCTGGGGCCCAAGCTGGTGCCGCTG
TTCTGCGGCGGTCCGACGCGCGCGGATTCGGTTCTGGCCGGCCTGCGCGCGATCGCCGGTGAGGCGGTGCAGAGCGACTG
GGTACTGGTGCATGACGCGGCGCGGCCTTGCCTCGCGCCCTGGCATATCGACAAGCTGGTGCGCGAACTCGCCGGCGACG
AGGTCGGCGGACTGCTTGCGGTGCCGGTGGCCGACACGCTGAAGCGCGCCGACGGCCACCGCCATGTGGCGGCGACGGTG
CCGCGCGACAGCCTGTGGCAGGCGCAGACGCCGCAGATGTTCCGCTACGTGATGCTGCGCCGCGCGCTGGAAAGCGCTAC
CGACGTTACCGACGAGGCAAGCGCGATCGAAGCGGCCGGCCTGCGGCCACGGCTGGTGCAGGGTGACGCGACCAACCTGA
AGGTGACCTATCCGCTCGACCTCCACCTGGCCGAGTGGATTCTCAACAACCGTACTGGAGTGCAGCAATGA

Upstream 100 bases:

>100_bases
GTGGTTTGGCGTGGGCGGGAGGTGCCAGCACTGTAGTCGATGCGGGTGGAAGCGGAAACCGGCTGCGGGCGCGGCGGCTG
GCGCGGTTAGAATCCGCGCC

Downstream 100 bases:

>100_bases
AATGTCCGTTCCGCGTCGGCCAGGGCTTCGACGTACACGCGCTGGTGCCGGGCCGGGCGCTGATCGTCGGGGGGGTCGAT
ATTCCGTTCTCCGCCGGGCT

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MQNFHPRHFAIVPAAGSGSRMGSERPKQYLPLLGKPLIYHTLSVLCAAPEIDKVFVVLSVGDAEWGRHDWRALGPKLVPL
FCGGPTRADSVLAGLRAIAGEAVQSDWVLVHDAARPCLAPWHIDKLVRELAGDEVGGLLAVPVADTLKRADGHRHVAATV
PRDSLWQAQTPQMFRYVMLRRALESATDVTDEASAIEAAGLRPRLVQGDATNLKVTYPLDLHLAEWILNNRTGVQQ

Sequences:

>Translated_236_residues
MQNFHPRHFAIVPAAGSGSRMGSERPKQYLPLLGKPLIYHTLSVLCAAPEIDKVFVVLSVGDAEWGRHDWRALGPKLVPL
FCGGPTRADSVLAGLRAIAGEAVQSDWVLVHDAARPCLAPWHIDKLVRELAGDEVGGLLAVPVADTLKRADGHRHVAATV
PRDSLWQAQTPQMFRYVMLRRALESATDVTDEASAIEAAGLRPRLVQGDATNLKVTYPLDLHLAEWILNNRTGVQQ
>Mature_236_residues
MQNFHPRHFAIVPAAGSGSRMGSERPKQYLPLLGKPLIYHTLSVLCAAPEIDKVFVVLSVGDAEWGRHDWRALGPKLVPL
FCGGPTRADSVLAGLRAIAGEAVQSDWVLVHDAARPCLAPWHIDKLVRELAGDEVGGLLAVPVADTLKRADGHRHVAATV
PRDSLWQAQTPQMFRYVMLRRALESATDVTDEASAIEAAGLRPRLVQGDATNLKVTYPLDLHLAEWILNNRTGVQQ

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=240, Percent_Identity=29.1666666666667, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI157671913, Length=118, Percent_Identity=34.7457627118644, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1789104, Length=237, Percent_Identity=47.2573839662447, Blast_Score=172, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_AZOSB (A1K644)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_933186.1
- ProteinModelPortal:   A1K644
- SMR:   A1K644
- STRING:   A1K644
- GeneID:   4606974
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo1682
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   FPQGAIL
- PhylomeDB:   A1K644
- ProtClustDB:   PRK00155
- BioCyc:   ASP62928:AZO1682-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 25778; Mature: 25778

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNFHPRHFAIVPAAGSGSRMGSERPKQYLPLLGKPLIYHTLSVLCAAPEIDKVFVVLSV
CCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEEEE
GDAEWGRHDWRALGPKLVPLFCGGPTRADSVLAGLRAIAGEAVQSDWVLVHDAARPCLAP
CCCCCCCHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCH
WHIDKLVRELAGDEVGGLLAVPVADTLKRADGHRHVAATVPRDSLWQAQTPQMFRYVMLR
HHHHHHHHHHCCCCCCCEEEECHHHHHHHCCCCCEEEEECCCHHCCCCCCHHHHHHHHHH
RALESATDVTDEASAIEAAGLRPRLVQGDATNLKVTYPLDLHLAEWILNNRTGVQQ
HHHHHHHCCCCHHHHHHHCCCCCEEECCCCCCEEEEEEHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQNFHPRHFAIVPAAGSGSRMGSERPKQYLPLLGKPLIYHTLSVLCAAPEIDKVFVVLSV
CCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEEEE
GDAEWGRHDWRALGPKLVPLFCGGPTRADSVLAGLRAIAGEAVQSDWVLVHDAARPCLAP
CCCCCCCHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCH
WHIDKLVRELAGDEVGGLLAVPVADTLKRADGHRHVAATVPRDSLWQAQTPQMFRYVMLR
HHHHHHHHHHCCCCCCCEEEECHHHHHHHCCCCCEEEEECCCHHCCCCCCHHHHHHHHHH
RALESATDVTDEASAIEAAGLRPRLVQGDATNLKVTYPLDLHLAEWILNNRTGVQQ
HHHHHHHCCCCHHHHHHHCCCCCEEECCCCCCEEEEEEHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA