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The map label for this gene is pdxJ

Identifier: 119897936

GI number: 119897936

Start: 1804870

End: 1805619

Strand: Direct

Name: pdxJ

Synonym: azo1645

Alternate gene names: 119897936

Gene position: 1804870-1805619 (Clockwise)

Preceding gene: 119897935

Following gene: 119897937

Centisome position: 41.24

GC content: 67.73

Gene sequence:

>750_bases
GTGATCGAACTCGGCGTCAATATCGACCACGTCGCCACGCTGCGCCAGGCGCGCCGCACCTGGGAGCCGGACCCGACCTG
GGCCGCGGTAGAAGCCCATCTCGGTGGAGCGGACGGCATCACCGTGCATCTGCGCGAAGACCGCCGTCACATCCAGGACG
ACGACGTCCGCCGCCTGCGCGAACTCACCCAGATCAAGTTGAACCTCGAGATGGCGGCCACCGACGAGATGGTCGGTATC
GCGCGCAGCATCAAGCCGGAAATGGCGATGCTGGTGCCGGAGGGCCGCCACGAGGTCACCACCGAAGGCGGGTTGGACAT
CGTGTCGCAGGAAGCGCGCCTGAAGGACGTGATCGCCCGGCTGGCGGACGCCGGCATCGTCACCAGTGTCTTCATCGATG
CGGAGATCGCCCAGATCGACGCCGCCGCGCGCATCGGCGCCAGGGTGTGCGAGATCCACACCGGCCCCTATGCCCACGCC
TTTCACGCCCAGGGGCGCGATGCTGAAAGCCCGGCGGTGCTGGCCGAGATCGACAAGATCCGCCGCGCCGGCGAAGCCAT
CTGCAGCCACGGCATGCGCTTCAACGCCGGCCATGCGCTCAACTACTACAACGTGCAACCGATTGCCCGGTTGCCCGGCA
TCCGCGAACTGCACATCGGCCACGCCATCGTCAGCCGCGCGGTCTTTTCCGGCATCCGCGAGGCGGTAGCGGAGATGAAG
CGTCTGATGCGCGAGGCGGCCGCCGGCTGA

Upstream 100 bases:

>100_bases
CGCTGGCCCAGTCCAAGCGGCTGCTGCGTGTGTTGATCAATCACTATCTCGGCGGACAGCCGCTGCAGTCCCGCCGCGTC
CTTCAGGAGTTGCTCGAACT

Downstream 100 bases:

>100_bases
GCCGGGGAGGGCGCATGATCCACGGCATCGGAACCGACATCGTCCACATCGCCCGCATCCGGACCTCGCTGGAGCGTCAC
GGCGAGCGCTTCGCGGAGCG

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MIELGVNIDHVATLRQARRTWEPDPTWAAVEAHLGGADGITVHLREDRRHIQDDDVRRLRELTQIKLNLEMAATDEMVGI
ARSIKPEMAMLVPEGRHEVTTEGGLDIVSQEARLKDVIARLADAGIVTSVFIDAEIAQIDAAARIGARVCEIHTGPYAHA
FHAQGRDAESPAVLAEIDKIRRAGEAICSHGMRFNAGHALNYYNVQPIARLPGIRELHIGHAIVSRAVFSGIREAVAEMK
RLMREAAAG

Sequences:

>Translated_249_residues
MIELGVNIDHVATLRQARRTWEPDPTWAAVEAHLGGADGITVHLREDRRHIQDDDVRRLRELTQIKLNLEMAATDEMVGI
ARSIKPEMAMLVPEGRHEVTTEGGLDIVSQEARLKDVIARLADAGIVTSVFIDAEIAQIDAAARIGARVCEIHTGPYAHA
FHAQGRDAESPAVLAEIDKIRRAGEAICSHGMRFNAGHALNYYNVQPIARLPGIRELHIGHAIVSRAVFSGIREAVAEMK
RLMREAAAG
>Mature_249_residues
MIELGVNIDHVATLRQARRTWEPDPTWAAVEAHLGGADGITVHLREDRRHIQDDDVRRLRELTQIKLNLEMAATDEMVGI
ARSIKPEMAMLVPEGRHEVTTEGGLDIVSQEARLKDVIARLADAGIVTSVFIDAEIAQIDAAARIGARVCEIHTGPYAHA
FHAQGRDAESPAVLAEIDKIRRAGEAICSHGMRFNAGHALNYYNVQPIARLPGIRELHIGHAIVSRAVFSGIREAVAEMK
RLMREAAAG

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=243, Percent_Identity=57.201646090535, Blast_Score=256, Evalue=1e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_AZOSB (A1K607)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_933149.1
- ProteinModelPortal:   A1K607
- SMR:   A1K607
- STRING:   A1K607
- GeneID:   4609709
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo1645
- eggNOG:   COG0854
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- PhylomeDB:   A1K607
- ProtClustDB:   PRK05265
- BioCyc:   ASP62928:AZO1645-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 27315; Mature: 27315

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: ACT_SITE 43-43 ACT_SITE 70-70 ACT_SITE 198-198 BINDING 7-7 BINDING 18-18 BINDING 45-45 BINDING 50-50 BINDING 100-100 BINDING 199-199

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELGVNIDHVATLRQARRTWEPDPTWAAVEAHLGGADGITVHLREDRRHIQDDDVRRLR
CEEECCCHHHHHHHHHHHHCCCCCCCCEEHEEECCCCCCEEEEEECCCCCCCCHHHHHHH
ELTQIKLNLEMAATDEMVGIARSIKPEMAMLVPEGRHEVTTEGGLDIVSQEARLKDVIAR
HHHHHHHEEEEHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LADAGIVTSVFIDAEIAQIDAAARIGARVCEIHTGPYAHAFHAQGRDAESPAVLAEIDKI
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEECCCCCCCCCCCHHHHHHHHH
RRAGEAICSHGMRFNAGHALNYYNVQPIARLPGIRELHIGHAIVSRAVFSGIREAVAEMK
HHHHHHHHHCCCEECCCCEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RLMREAAAG
HHHHHHCCC
>Mature Secondary Structure
MIELGVNIDHVATLRQARRTWEPDPTWAAVEAHLGGADGITVHLREDRRHIQDDDVRRLR
CEEECCCHHHHHHHHHHHHCCCCCCCCEEHEEECCCCCCEEEEEECCCCCCCCHHHHHHH
ELTQIKLNLEMAATDEMVGIARSIKPEMAMLVPEGRHEVTTEGGLDIVSQEARLKDVIAR
HHHHHHHEEEEHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LADAGIVTSVFIDAEIAQIDAAARIGARVCEIHTGPYAHAFHAQGRDAESPAVLAEIDKI
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEECCCCCCCCCCCHHHHHHHHH
RRAGEAICSHGMRFNAGHALNYYNVQPIARLPGIRELHIGHAIVSRAVFSGIREAVAEMK
HHHHHHHHHCCCEECCCCEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RLMREAAAG
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA