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The map label for this gene is gacA [H]

Identifier: 119897794

GI number: 119897794

Start: 1644105

End: 1644740

Strand: Reverse

Name: gacA [H]

Synonym: azo1503

Alternate gene names: 119897794

Gene position: 1644740-1644105 (Counterclockwise)

Preceding gene: 119897795

Following gene: 119897790

Centisome position: 37.59

GC content: 72.48

Gene sequence:

>636_bases
ATGATCCGCCTGCTGCTGGTGGACGACCACGCAGTGGTGCGCCAGGGCTATCGCCGCCTGCTCGAACAGCAGGGCAACCT
CGTGGTCGCCGGCGAGGCCGCCACCGGCGCGGACGGCCTGCAGCTGTGGCGCGAACTGCAGCCGGACCTCGCGGTGCTCG
ACCTCGCGCTGCCCGACATCGGCGGCCTCGAACTCCTCGCCCGCATCCGCCAGCGCGACCCGCAGGCCCGCCTGCTCGCC
TTCAGCATGCATCGCGACGTGTTGTGGGCGGTGCAGGCAGTGCGCGCCGGCGCGCTCGGCTACGTCACCAAGAGCAGCCC
GCCGGAGGTGTTGCAGCAGGCGGTGCGCGAGGTGGCGGGCGGACGCCGCTTCTTCAGCCCGGACATCGCCACCGAACTGC
GCGCCGCGCTGCGCGAAGCCGAGCCGCCTACCGCCGCCGACTGCCTCAGCCCGCGCGAACTCGAAGTGCTGCGCCTGCTG
CTGGCCGGCACCCCGGTGGCGGAGATTGCCACGGCGCTGTGCCTGAGCCCGAAGACGGTGCACAACACCCACTACCAGAT
CAAGGCGCGGCTGGGCGCCGGGAATGATTTCGAGCTGGTGCGGATCGCGCGGCGGCTGGGGCTGGGGGAGGACTGA

Upstream 100 bases:

>100_bases
GGGTCGCGGCCCTGGGCGGCAGCGTGGAACTCGCGCCGCTGGCCACCGGCGGCATGCGGCTCGCCGCCGCATGGCCACTG
CCCGCGACGGAGGTGGCGGC

Downstream 100 bases:

>100_bases
GGCCGCGCCGACCCCATCCCCACCCCTGCCCGGGAGTCGTCGCTTCGCCCCGCCGCTCCGGCGGCGCAGAGCGATGCTCT
GCGAAACCCCGCCTACGCCC

Product: putative two-component response regulator

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MIRLLLVDDHAVVRQGYRRLLEQQGNLVVAGEAATGADGLQLWRELQPDLAVLDLALPDIGGLELLARIRQRDPQARLLA
FSMHRDVLWAVQAVRAGALGYVTKSSPPEVLQQAVREVAGGRRFFSPDIATELRAALREAEPPTAADCLSPRELEVLRLL
LAGTPVAEIATALCLSPKTVHNTHYQIKARLGAGNDFELVRIARRLGLGED

Sequences:

>Translated_211_residues
MIRLLLVDDHAVVRQGYRRLLEQQGNLVVAGEAATGADGLQLWRELQPDLAVLDLALPDIGGLELLARIRQRDPQARLLA
FSMHRDVLWAVQAVRAGALGYVTKSSPPEVLQQAVREVAGGRRFFSPDIATELRAALREAEPPTAADCLSPRELEVLRLL
LAGTPVAEIATALCLSPKTVHNTHYQIKARLGAGNDFELVRIARRLGLGED
>Mature_211_residues
MIRLLLVDDHAVVRQGYRRLLEQQGNLVVAGEAATGADGLQLWRELQPDLAVLDLALPDIGGLELLARIRQRDPQARLLA
FSMHRDVLWAVQAVRAGALGYVTKSSPPEVLQQAVREVAGGRRFFSPDIATELRAALREAEPPTAADCLSPRELEVLRLL
LAGTPVAEIATALCLSPKTVHNTHYQIKARLGAGNDFELVRIARRLGLGED

Specific function: Positively controls the production of the autoinducer N- butyryl-homoserine lactone and the formation of the virulence factors pyocyanine, cyanide, and lipase [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=211, Percent_Identity=36.0189573459716, Blast_Score=141, Evalue=3e-35,
Organism=Escherichia coli, GI1790102, Length=202, Percent_Identity=36.1386138613861, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787473, Length=179, Percent_Identity=35.7541899441341, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1786747, Length=201, Percent_Identity=27.8606965174129, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788521, Length=179, Percent_Identity=32.9608938547486, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788712, Length=205, Percent_Identity=24.390243902439, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788546, Length=204, Percent_Identity=27.4509803921569, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87082052, Length=104, Percent_Identity=29.8076923076923, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23059; Mature: 23059

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRLLLVDDHAVVRQGYRRLLEQQGNLVVAGEAATGADGLQLWRELQPDLAVLDLALPDI
CEEEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCEEHHHHCCCC
GGLELLARIRQRDPQARLLAFSMHRDVLWAVQAVRAGALGYVTKSSPPEVLQQAVREVAG
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCHHEECCCCCCHHHHHHHHHHHHC
GRRFFSPDIATELRAALREAEPPTAADCLSPRELEVLRLLLAGTPVAEIATALCLSPKTV
CCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHH
HNTHYQIKARLGAGNDFELVRIARRLGLGED
CCCCEEEEEEECCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIRLLLVDDHAVVRQGYRRLLEQQGNLVVAGEAATGADGLQLWRELQPDLAVLDLALPDI
CEEEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCEEHHHHCCCC
GGLELLARIRQRDPQARLLAFSMHRDVLWAVQAVRAGALGYVTKSSPPEVLQQAVREVAG
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCHHEECCCCCCHHHHHHHHHHHHC
GRRFFSPDIATELRAALREAEPPTAADCLSPRELEVLRLLLAGTPVAEIATALCLSPKTV
CCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHH
HNTHYQIKARLGAGNDFELVRIARRLGLGED
CCCCEEEEEEECCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9159518; 10984043 [H]