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The map label for this gene is pfkA [H]

Identifier: 119897765

GI number: 119897765

Start: 1605395

End: 1606660

Strand: Direct

Name: pfkA [H]

Synonym: azo1474

Alternate gene names: 119897765

Gene position: 1605395-1606660 (Clockwise)

Preceding gene: 119897764

Following gene: 119897766

Centisome position: 36.69

GC content: 70.93

Gene sequence:

>1266_bases
ATGAGCCCGACCCAGCCGTTCAATCTCCTCTACGCCCAGGCGGGCGGCGTCACCGCGGTGATCAACGCGAGCGCTGCCGC
GGTCATCGAAGCCGCGCGCGAACATCCGCAGGCGATCGGCACTGTCTATGGCGCGCGCAACGGCATTCTCGGGGTGCTGA
GTGAAACGCTGGTCGATACGACGCCGCTGACGCCCGCCGAGCTGGCACAGCTTGCACTCACGCCCGGCGGCGCATTCGGT
TCGTGCCGCTTCGACCTCGATCCGGCGGAAGCCAATCCCGCGCAGTACGACCGTCTGTTCGAGGTGTTCGCCAGCTATCG
CATCGGCGCCTTCCTGTACAACGGCGGCAACGGTTCCATGGACATCGTCGCCCGCGTTGCCGCAGCGGCGCGCGCGCGGG
CTTACCCGCTGTGCTGCATCGGTGTGCCCAAGACGGTGGACAACGACCTCGAGGGCACCGACTGCTCGCCCGGCTTCGGC
TCCGCGGCCAAGTATCTCGCCACCGCGATGCGCGAGTCCGGGCTGGATTTGGCGGCGATGTCCAGCCGGCGTGGGCGGGT
GTTCGTCATGGAAGTGATGGGGCGCAACGCCGGCTGGCTGGCTGCCGCCACCGCGCTGGCCGCGCGCAACGAGGACGAGC
CGCCGCACATCGTGCTGCTGCCCGAGGTCGCGTTCGACGAGGAGGCCTTCCTCGCGCGGGTGAAGGCGGTGGTCGAGCGC
CTGGGCTACTGCGCAGTCACCGTGGCGGAAGGCATCCGCCGCGCCGACGGCACGCTGGTGATGGAGCAGTCGCACGACGC
GCTCGGTCACGTCCAGTTGGGCGGCGCGGGGCAGTGCGTCGCGCGCTTGGTTTCGGCGCGCTTCGACTACAAGCACCACT
GGGCCATCCCGGACTACCTGCAGCGCGCGGCCGGCCACTGGCGCTCGGCCACCGACGTGGCGCAGGCAAGGGCGGTGGGG
CGGGCTGCGGTGGCCTACGCGCTGGCCGGGCAGGATGGGGTGATGCCGGCGATCCGCCGCCTGGCGGACGAACCGTATCG
GTGGGATGTGCAGCCGGTGCGGGTGGAGGAGATCGCCAACCTCGAGCGCCGCGTGCCGCCGCATTTCATCGCCGCCGACG
GCCTGCACGTCACCGCCGCCGCCAAGGCCGCGCTGGCGCCGCTGATCGAAGGCGAACTGCCGGTGCCGACCCACGCCGGT
CTGCCCGACTACTTCCAACTGCAACTCGCACCCCAACCGCGCCGGCTGCCTGCCTGGACGCGCTGA

Upstream 100 bases:

>100_bases
CGTCATCCGACGCGGTCGGTACCGAACCCGGTGCCGGCCGCGTTTGTCTTTGCAGGTGTGATCCGGCGCATGCGCGCCGC
TGTCGAGGTGACCTCCTACG

Downstream 100 bases:

>100_bases
GCGCGGGCGGCAGGCCGATGCGCGCGGCGATCCTGTTCGCGGTCTGGCTGTGGCTTGCGGCCACGGCGGCCGCGCAGCCG
CCCGCGCCCGAGGCCGCGAC

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: ATP-PFK; Phosphofructokinase 1; Phosphohexokinase 1 [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MSPTQPFNLLYAQAGGVTAVINASAAAVIEAAREHPQAIGTVYGARNGILGVLSETLVDTTPLTPAELAQLALTPGGAFG
SCRFDLDPAEANPAQYDRLFEVFASYRIGAFLYNGGNGSMDIVARVAAAARARAYPLCCIGVPKTVDNDLEGTDCSPGFG
SAAKYLATAMRESGLDLAAMSSRRGRVFVMEVMGRNAGWLAAATALAARNEDEPPHIVLLPEVAFDEEAFLARVKAVVER
LGYCAVTVAEGIRRADGTLVMEQSHDALGHVQLGGAGQCVARLVSARFDYKHHWAIPDYLQRAAGHWRSATDVAQARAVG
RAAVAYALAGQDGVMPAIRRLADEPYRWDVQPVRVEEIANLERRVPPHFIAADGLHVTAAAKAALAPLIEGELPVPTHAG
LPDYFQLQLAPQPRRLPAWTR

Sequences:

>Translated_421_residues
MSPTQPFNLLYAQAGGVTAVINASAAAVIEAAREHPQAIGTVYGARNGILGVLSETLVDTTPLTPAELAQLALTPGGAFG
SCRFDLDPAEANPAQYDRLFEVFASYRIGAFLYNGGNGSMDIVARVAAAARARAYPLCCIGVPKTVDNDLEGTDCSPGFG
SAAKYLATAMRESGLDLAAMSSRRGRVFVMEVMGRNAGWLAAATALAARNEDEPPHIVLLPEVAFDEEAFLARVKAVVER
LGYCAVTVAEGIRRADGTLVMEQSHDALGHVQLGGAGQCVARLVSARFDYKHHWAIPDYLQRAAGHWRSATDVAQARAVG
RAAVAYALAGQDGVMPAIRRLADEPYRWDVQPVRVEEIANLERRVPPHFIAADGLHVTAAAKAALAPLIEGELPVPTHAG
LPDYFQLQLAPQPRRLPAWTR
>Mature_420_residues
SPTQPFNLLYAQAGGVTAVINASAAAVIEAAREHPQAIGTVYGARNGILGVLSETLVDTTPLTPAELAQLALTPGGAFGS
CRFDLDPAEANPAQYDRLFEVFASYRIGAFLYNGGNGSMDIVARVAAAARARAYPLCCIGVPKTVDNDLEGTDCSPGFGS
AAKYLATAMRESGLDLAAMSSRRGRVFVMEVMGRNAGWLAAATALAARNEDEPPHIVLLPEVAFDEEAFLARVKAVVERL
GYCAVTVAEGIRRADGTLVMEQSHDALGHVQLGGAGQCVARLVSARFDYKHHWAIPDYLQRAAGHWRSATDVAQARAVGR
AAVAYALAGQDGVMPAIRRLADEPYRWDVQPVRVEEIANLERRVPPHFIAADGLHVTAAAKAALAPLIEGELPVPTHAGL
PDYFQLQLAPQPRRLPAWTR

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Escherichia coli, GI1790350, Length=287, Percent_Identity=28.2229965156794, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 44867; Mature: 44735

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPTQPFNLLYAQAGGVTAVINASAAAVIEAAREHPQAIGTVYGARNGILGVLSETLVDT
CCCCCCCEEEEECCCCEEEEECCHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHCC
TPLTPAELAQLALTPGGAFGSCRFDLDPAEANPAQYDRLFEVFASYRIGAFLYNGGNGSM
CCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCEEEEEEECCCCCH
DIVARVAAAARARAYPLCCIGVPKTVDNDLEGTDCSPGFGSAAKYLATAMRESGLDLAAM
HHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEC
SSRRGRVFVMEVMGRNAGWLAAATALAARNEDEPPHIVLLPEVAFDEEAFLARVKAVVER
CCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
LGYCAVTVAEGIRRADGTLVMEQSHDALGHVQLGGAGQCVARLVSARFDYKHHWAIPDYL
HCHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCHHHH
QRAAGHWRSATDVAQARAVGRAAVAYALAGQDGVMPAIRRLADEPYRWDVQPVRVEEIAN
HHHHCCCCCHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHCCCCCCCCCCEECHHHHHH
LERRVPPHFIAADGLHVTAAAKAALAPLIEGELPVPTHAGLPDYFQLQLAPQPRRLPAWT
HHHCCCCCEEECCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCC
R
C
>Mature Secondary Structure 
SPTQPFNLLYAQAGGVTAVINASAAAVIEAAREHPQAIGTVYGARNGILGVLSETLVDT
CCCCCCEEEEECCCCEEEEECCHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHCC
TPLTPAELAQLALTPGGAFGSCRFDLDPAEANPAQYDRLFEVFASYRIGAFLYNGGNGSM
CCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCEEEEEEECCCCCH
DIVARVAAAARARAYPLCCIGVPKTVDNDLEGTDCSPGFGSAAKYLATAMRESGLDLAAM
HHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEC
SSRRGRVFVMEVMGRNAGWLAAATALAARNEDEPPHIVLLPEVAFDEEAFLARVKAVVER
CCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
LGYCAVTVAEGIRRADGTLVMEQSHDALGHVQLGGAGQCVARLVSARFDYKHHWAIPDYL
HCHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCHHHH
QRAAGHWRSATDVAQARAVGRAAVAYALAGQDGVMPAIRRLADEPYRWDVQPVRVEEIAN
HHHHCCCCCHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHCCCCCCCCCCEECHHHHHH
LERRVPPHFIAADGLHVTAAAKAALAPLIEGELPVPTHAGLPDYFQLQLAPQPRRLPAWT
HHHCCCCCEEECCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9055413; 12000953 [H]