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The map label for this gene is carA [H]

Identifier: 119897672

GI number: 119897672

Start: 1508792

End: 1509934

Strand: Direct

Name: carA [H]

Synonym: azo1381

Alternate gene names: 119897672

Gene position: 1508792-1509934 (Clockwise)

Preceding gene: 119897671

Following gene: 119897673

Centisome position: 34.48

GC content: 65.53

Gene sequence:

>1143_bases
GTGACTGCTTTCCCGCCCGCCCTTCTTGCGCTTGCCGACGGGACCGTCTTCTACGGCAAGGGTATCGGAGCGGTAGGCAG
CACGGTTGGCGAAGTTGTTTTCAATACCTCCATGTCGGGCTACCAGGAAATCCTGACCGACCCGAGCTATTGCCGCCAGA
TCGTGACGCTTACCTATCCGCACATCGGCAACACCGGCGTCAATCGCGAGGACGTCGAGGCTGACCGTGTGCACGCGGCC
GGGCTGGTCATTCGCGACCTTCCCATCCTGCCGTCCAACTTCCGCATGGATCAGCGCCTCGACGCCTACCTCAAGGCCGA
GAACGTCGTGGCGATTGCCGGTCTCGATACCCGCAAGCTCACGCGCATCCTGCGCGAGAAGGGTGCGCAGGCCGGCTGCA
TCGTCACGGCGGCCGACGGCGCGCAGCTCAATGCCGAAGCGGCGGTGGCCGAGGCGCGCGCCTTTCCCGGGCTGGCCGGC
ATGGACCTCGCAAAGGTCGTCAGTGCCAAGGAGGCTTATCCCTGGCAGGAAGGCGAGTGGGTGCTTGGTGAAGGCTATGT
GGCCCAAACCGAGCCGCGTTTCCATGTCGTGGCCTACGATTTCGGCGTCAAGCGCAACATCCTGCGCATGCTGGCGCAGC
GCGGTTGCCGTCTGACGGTGGTGCCCGCCCAGACGCCGGCCACCGTCGTGCTCGGGCTGAAGCCGGACGGCGTGTTCCTG
TCCAACGGTCCTGGCGATCCGGAGCCCTGCGATTACGCGATCGAGGCGATCCGCGAAATCCTCGCCGCACGCGTGCCCAC
CTTCGGCATCTGTCTCGGCCACCAGTTGCTGGCGCTTGCCTCGGGCGGACGTACCGTCAAGATGAAGGCGGGGCACCACG
GTGCCAACCATCCGGTGAAGGACGTCGATAGCGGTCAGGTCCTGATCACCAGTCAGAACCACGGTTTCGCGGTCGATGTC
GCCAACCTGCCGTCCAGTCTGCGCGTCACCCACGTGTCGCTGTTCGACGGCTCCAACCAGGGTATCGCCCGTACCGACGT
GCCGGCCTTCTCCTTCCAGGGGCACCCGGAGGCGAGCCCCGGCCCGCACGACGTCGCCTACCTGTTCGACCGCTTCATCG
GCTTGATGGAAGCGGCCAAGTAA

Upstream 100 bases:

>100_bases
ATTTGATAGCGGGATTGGCCCTTCGGGCTCGTCCCGTTTTTTCACATATGGGGGCGGCGGCTTGTGCCCCTGTTTTTTCG
GCTGAACTCAGGAGTTTGTC

Downstream 100 bases:

>100_bases
CACTGCATCCCCGGCGGGCGCGGCCCGCCGTCCCAGATTTACAGAGGCAAGGCAATGCCGAAACGTACCGACATTAAGAG
CATCCTGATCATCGGCGCTG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MTAFPPALLALADGTVFYGKGIGAVGSTVGEVVFNTSMSGYQEILTDPSYCRQIVTLTYPHIGNTGVNREDVEADRVHAA
GLVIRDLPILPSNFRMDQRLDAYLKAENVVAIAGLDTRKLTRILREKGAQAGCIVTAADGAQLNAEAAVAEARAFPGLAG
MDLAKVVSAKEAYPWQEGEWVLGEGYVAQTEPRFHVVAYDFGVKRNILRMLAQRGCRLTVVPAQTPATVVLGLKPDGVFL
SNGPGDPEPCDYAIEAIREILAARVPTFGICLGHQLLALASGGRTVKMKAGHHGANHPVKDVDSGQVLITSQNHGFAVDV
ANLPSSLRVTHVSLFDGSNQGIARTDVPAFSFQGHPEASPGPHDVAYLFDRFIGLMEAAK

Sequences:

>Translated_380_residues
MTAFPPALLALADGTVFYGKGIGAVGSTVGEVVFNTSMSGYQEILTDPSYCRQIVTLTYPHIGNTGVNREDVEADRVHAA
GLVIRDLPILPSNFRMDQRLDAYLKAENVVAIAGLDTRKLTRILREKGAQAGCIVTAADGAQLNAEAAVAEARAFPGLAG
MDLAKVVSAKEAYPWQEGEWVLGEGYVAQTEPRFHVVAYDFGVKRNILRMLAQRGCRLTVVPAQTPATVVLGLKPDGVFL
SNGPGDPEPCDYAIEAIREILAARVPTFGICLGHQLLALASGGRTVKMKAGHHGANHPVKDVDSGQVLITSQNHGFAVDV
ANLPSSLRVTHVSLFDGSNQGIARTDVPAFSFQGHPEASPGPHDVAYLFDRFIGLMEAAK
>Mature_379_residues
TAFPPALLALADGTVFYGKGIGAVGSTVGEVVFNTSMSGYQEILTDPSYCRQIVTLTYPHIGNTGVNREDVEADRVHAAG
LVIRDLPILPSNFRMDQRLDAYLKAENVVAIAGLDTRKLTRILREKGAQAGCIVTAADGAQLNAEAAVAEARAFPGLAGM
DLAKVVSAKEAYPWQEGEWVLGEGYVAQTEPRFHVVAYDFGVKRNILRMLAQRGCRLTVVPAQTPATVVLGLKPDGVFLS
NGPGDPEPCDYAIEAIREILAARVPTFGICLGHQLLALASGGRTVKMKAGHHGANHPVKDVDSGQVLITSQNHGFAVDVA
NLPSSLRVTHVSLFDGSNQGIARTDVPAFSFQGHPEASPGPHDVAYLFDRFIGLMEAAK

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=386, Percent_Identity=38.860103626943, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI21361331, Length=389, Percent_Identity=34.4473007712082, Blast_Score=211, Evalue=8e-55,
Organism=Homo sapiens, GI169790915, Length=389, Percent_Identity=34.4473007712082, Blast_Score=211, Evalue=9e-55,
Organism=Escherichia coli, GI1786215, Length=374, Percent_Identity=68.9839572192513, Blast_Score=528, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI193204318, Length=393, Percent_Identity=38.1679389312977, Blast_Score=247, Evalue=6e-66,
Organism=Saccharomyces cerevisiae, GI6322331, Length=396, Percent_Identity=38.6363636363636, Blast_Score=242, Evalue=6e-65,
Organism=Saccharomyces cerevisiae, GI6324878, Length=386, Percent_Identity=38.60103626943, Blast_Score=229, Evalue=4e-61,
Organism=Drosophila melanogaster, GI45555749, Length=384, Percent_Identity=39.0625, Blast_Score=224, Evalue=9e-59,
Organism=Drosophila melanogaster, GI24642586, Length=384, Percent_Identity=39.0625, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40466; Mature: 40334

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAFPPALLALADGTVFYGKGIGAVGSTVGEVVFNTSMSGYQEILTDPSYCRQIVTLTYP
CCCCCCHHHEEECCCEEECCCCCHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHEEECC
HIGNTGVNREDVEADRVHAAGLVIRDLPILPSNFRMDQRLDAYLKAENVVAIAGLDTRKL
CCCCCCCCCCCCCHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHHHCCEEEEECCCHHHH
TRILREKGAQAGCIVTAADGAQLNAEAAVAEARAFPGLAGMDLAKVVSAKEAYPWQEGEW
HHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCE
VLGEGYVAQTEPRFHVVAYDFGVKRNILRMLAQRGCRLTVVPAQTPATVVLGLKPDGVFL
EEECCEEEECCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCCCEEE
SNGPGDPEPCDYAIEAIREILAARVPTFGICLGHQLLALASGGRTVKMKAGHHGANHPVK
ECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
DVDSGQVLITSQNHGFAVDVANLPSSLRVTHVSLFDGSNQGIARTDVPAFSFQGHPEASP
CCCCCEEEEEECCCCEEEECCCCCCCEEEEEEEEEECCCCCCEECCCCCEECCCCCCCCC
GPHDVAYLFDRFIGLMEAAK
CCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAFPPALLALADGTVFYGKGIGAVGSTVGEVVFNTSMSGYQEILTDPSYCRQIVTLTYP
CCCCCHHHEEECCCEEECCCCCHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHEEECC
HIGNTGVNREDVEADRVHAAGLVIRDLPILPSNFRMDQRLDAYLKAENVVAIAGLDTRKL
CCCCCCCCCCCCCHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHHHCCEEEEECCCHHHH
TRILREKGAQAGCIVTAADGAQLNAEAAVAEARAFPGLAGMDLAKVVSAKEAYPWQEGEW
HHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCE
VLGEGYVAQTEPRFHVVAYDFGVKRNILRMLAQRGCRLTVVPAQTPATVVLGLKPDGVFL
EEECCEEEECCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCCCEEE
SNGPGDPEPCDYAIEAIREILAARVPTFGICLGHQLLALASGGRTVKMKAGHHGANHPVK
ECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
DVDSGQVLITSQNHGFAVDVANLPSSLRVTHVSLFDGSNQGIARTDVPAFSFQGHPEASP
CCCCCEEEEEECCCCEEEECCCCCCCEEEEEEEEEECCCCCCEECCCCCEECCCCCCCCC
GPHDVAYLFDRFIGLMEAAK
CCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]