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The map label for this gene is vfr [H]

Identifier: 119897655

GI number: 119897655

Start: 1484423

End: 1485094

Strand: Direct

Name: vfr [H]

Synonym: azo1364

Alternate gene names: 119897655

Gene position: 1484423-1485094 (Clockwise)

Preceding gene: 119897652

Following gene: 119897656

Centisome position: 33.92

GC content: 62.65

Gene sequence:

>672_bases
ATGAGCCAGCCGACCGCTGTTTCGACGATCGCGCTCAAGACCTTTCCCTTGTTCCAGGGTTTGTCCGACGAGGTGCTCGG
CAACGTGGCCCGTGTTTCGATGATGCGCCGGATCCCGCGCGGCCAGGCCGTCGTCCGGGCCGGCGATCGCACCGACTACG
TTTATTTCGTCCTTACCGGCAATCTCAAGGTCGTCGTCAGCGACGAGGACGGCCGCGAAGTGATCCTGTCCATCCTCGGC
CAGGGCGAGCTTTTCGGCGAGATGGGCATGTTCGGCGAGCAGCCCCGCTCGGCTTCCGTGATCGCGGTGGTGCCGGCCGA
CCTGGTAATGATCGCGAAGCAGGACTTCCGCCAGATCATGCAGGAGAACTTCGAGATCGCGTGGCGCATCATGTCCAACC
TCGCCGAGCGCCTGCGCAACGCCGACCGCAAGATCGAGAGCCTCGCTCTGATGGACGTCTATGGCCGCGTCGCGCGGCTG
TTGATCGACATGTCGGAAGACGTGAACGGCAAGACCATGGTGGTGCGCAAGATCTCCAAGCAGGACATTGCCAAGATGAT
CGGCGCTTCCCGGGAGATGGTCAGCCGGGTGATGAAGGATCTCGGCCAGGAAGGCCTGATCGAGGAAACCCCTCAGGGCA
TCATCCTGCTCGACCGCCTCAACGAGGTCTGA

Upstream 100 bases:

>100_bases
AGGCCGTGTTTATTCGTTTATACTTTCGTCCAAATTGACCGCGACGGCCGCGTCGCATTACGTTTCCACCTTCGTTTGCT
TCGATTTGCCCGGAGAACCT

Downstream 100 bases:

>100_bases
CCGGGCTGCGCGGGCAGGGGCGCGCATTCGCGCCGCCGCTGTCCCGCGGATTGTGAGCAAGGGCAAACCGCGGCCGGGCA
GGTGGGGTGATCGCATATAA

Product: cyclic AMP receptor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MSQPTAVSTIALKTFPLFQGLSDEVLGNVARVSMMRRIPRGQAVVRAGDRTDYVYFVLTGNLKVVVSDEDGREVILSILG
QGELFGEMGMFGEQPRSASVIAVVPADLVMIAKQDFRQIMQENFEIAWRIMSNLAERLRNADRKIESLALMDVYGRVARL
LIDMSEDVNGKTMVVRKISKQDIAKMIGASREMVSRVMKDLGQEGLIEETPQGIILLDRLNEV

Sequences:

>Translated_223_residues
MSQPTAVSTIALKTFPLFQGLSDEVLGNVARVSMMRRIPRGQAVVRAGDRTDYVYFVLTGNLKVVVSDEDGREVILSILG
QGELFGEMGMFGEQPRSASVIAVVPADLVMIAKQDFRQIMQENFEIAWRIMSNLAERLRNADRKIESLALMDVYGRVARL
LIDMSEDVNGKTMVVRKISKQDIAKMIGASREMVSRVMKDLGQEGLIEETPQGIILLDRLNEV
>Mature_222_residues
SQPTAVSTIALKTFPLFQGLSDEVLGNVARVSMMRRIPRGQAVVRAGDRTDYVYFVLTGNLKVVVSDEDGREVILSILGQ
GELFGEMGMFGEQPRSASVIAVVPADLVMIAKQDFRQIMQENFEIAWRIMSNLAERLRNADRKIESLALMDVYGRVARLL
IDMSEDVNGKTMVVRKISKQDIAKMIGASREMVSRVMKDLGQEGLIEETPQGIILLDRLNEV

Specific function: Can bind cyclic AMP. Is a global regulator of virulence factor expression and is required for exotoxin A and protease production [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI148612818, Length=110, Percent_Identity=31.8181818181818, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI10835242, Length=110, Percent_Identity=31.8181818181818, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1789756, Length=199, Percent_Identity=32.1608040201005, Blast_Score=123, Evalue=9e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR018488
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 24863; Mature: 24732

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00889 CNMP_BINDING_2 ; PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
6.3 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPTAVSTIALKTFPLFQGLSDEVLGNVARVSMMRRIPRGQAVVRAGDRTDYVYFVLTG
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEEC
NLKVVVSDEDGREVILSILGQGELFGEMGMFGEQPRSASVIAVVPADLVMIAKQDFRQIM
CEEEEEECCCCHHHHHHHHCCCHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHH
QENFEIAWRIMSNLAERLRNADRKIESLALMDVYGRVARLLIDMSEDVNGKTMVVRKISK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
QDIAKMIGASREMVSRVMKDLGQEGLIEETPQGIILLDRLNEV
HHHHHHHCCCHHHHHHHHHHCCCCCCHHCCCCCEEEECCCCCC
>Mature Secondary Structure 
SQPTAVSTIALKTFPLFQGLSDEVLGNVARVSMMRRIPRGQAVVRAGDRTDYVYFVLTG
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEEC
NLKVVVSDEDGREVILSILGQGELFGEMGMFGEQPRSASVIAVVPADLVMIAKQDFRQIM
CEEEEEECCCCHHHHHHHHCCCHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHH
QENFEIAWRIMSNLAERLRNADRKIESLALMDVYGRVARLLIDMSEDVNGKTMVVRKISK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
QDIAKMIGASREMVSRVMKDLGQEGLIEETPQGIILLDRLNEV
HHHHHHHCCCHHHHHHHHHHCCCCCCHHCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8002577; 10984043 [H]