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The map label for this gene is phbC2 [H]

Identifier: 119897641

GI number: 119897641

Start: 1467151

End: 1468884

Strand: Direct

Name: phbC2 [H]

Synonym: azo1350

Alternate gene names: 119897641

Gene position: 1467151-1468884 (Clockwise)

Preceding gene: 119897639

Following gene: 119897643

Centisome position: 33.53

GC content: 61.53

Gene sequence:

>1734_bases
ATGAACAAGAGCGAGCGACTCGACCCAGGTCAAACCGTAAAGCGTTCGATCAAGCAACTGCACGAAGCGGTCGAGGGGGC
CTTCGATCCGCTCGGGATGGCGGCGCCTATCATTCACGCCCAGCTGGCTTGGGCTGCGCATCCGCAGGAGCTGGCAGATC
GTCTTCTGCGTCTTTCCTCCGACCTGTGGCGTCTGCAATGGCATTCCTGGAACCGGGTTCTCGGCCTCGACAGCGAAGAT
CCGGTGCGGCCGAATCCGGACGATGCGCGTTTTGCCGATCCGGTCTGGACTGAGTCGCCGACCTGGGATCTCACCAAAGA
GTGGTATCTGGCGCTGACCCACCATGTCCAGGACATGCTCTATGACACGCCGGGTCTGACGGGGAAGGAGCGGCGGCGGG
CGGCGTTCTGGTGGCGGAAATGGCTGAATGCGGTCGCGCCGACCAATTTCTTCTGGACGAATCCGGTCGCAGTGCGAAAG
GCGTGGGAAACGAAGGGTGAGAGCCTGGTCCGCGGGTTCGGCAATTTCCTCGACGACGTTCGCGCGGGTGAAGTGCGGAT
GACTCGTCCCGATGACTTCGAGGTGGGCAAGAACCTGGCGACGACGCCCGGCGCGGTCGTGTTCAGGAACGACCTCTTGG
AGGTGATTCATTACCAACCCACTCGTCCGCAAGTGCACCGCGATCCGGTCGTCATCGTCACGCCGTGGATCAACAAGTTC
TACATCCTCGATCTCGTTCCGAAGAAGAGCCTGATTCGTTACCTGCTCGACCAGGGACTGGACGTATTCATTACAAGCTG
GAAGAACCCTGACGCCTCGATGCGGGATGTGACGTTCGACGACTACGTCACACGCGGTGTGCACGCCGCGGTAGAGACGG
CGCGAGGGCTTGCGGGCGCGGAGAAGGTGCATGCGGTGGGATACTGCATCGGTGGCACGGCGCTCAGCATGTACATGGCG
TGGGCCAATCGCCACTTTCCGGCCGAGGCGGTTCCGGTGGCAGACTGGACCGTCTTTACCACGCTCGTCGATTTTCAGAA
GCCGGGCGACATCGAGGTGTTCATCGACGAGTCGAGCATCCGCTACCTGACCGGAAACATGGCCCGAACCGGTTATCTGG
AAGGCAAGGAGATGGCATCCGCTTTCCGCCTGCTGCGTTCGAACAGTCTCATATGGCACTACGTAGTCCACGGCTGGCTG
TACGGCGAAACACCGGCCTCGTTCGACGTGCTGTACTGGAACATGGACGCGACGCGGATGCCCTATGCGATGCACTCCTG
GTATCTCAGGGAGCTGTATCTGCACAACAGGTTGATTCAGAAGGACGCGCTGGTGGTGGCAGGCGAACCGCTCGACCTCG
CTGCGATCTCGCAGCCGCTGTACGCCGTCGCGGCGGAGGATGATCACATCGCTCCGTGGGGACAGACCTTCCGCATCAAT
AATTTTGTAACCGGCCCCAAGCGCTACGTGCTATCGAGTTCGGGACACATCCTCGGCATCGTCAACCCGGTCGTTCAGCC
GCCCAAGCGAAACTATCGCGCAGCCGAAGCGCATCGCTCGGAATCGGCTGAGGCATGGTTGGACCGGGCCGAACGCCACG
AGGGGAGTTGGTGGGAGGACTGGATGCAGTGGTTGAAGCCGCGGGCGGGCGAACTGGTCGACGCGCGACCGGTCGCCAAT
GCTGCCTTTCCGGCTTTGGCGCCGGCGCCAGGAACCTACGTGCTGGAAAAATAG

Upstream 100 bases:

>100_bases
GGGAACCTCCGCGTACCGTGGGAGTCATCACCGACGATGTTGGTTGGGCTGCCGTGGCAAAATGGAAAAGCCCCCTGTCA
TTTCGAAGCTTGGAGACGTG

Downstream 100 bases:

>100_bases
CGGTGAAGTGGGGCGGGAGGAACTAGCGACACGCAGGTTGGTGTTGTATCAGCTTCTTCAGGCCCCCGCTGTCGAGAATC
CGGATGTGCTTCTGCTGCAC

Product: poly-beta-hydroxybutyrate synthase

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MNKSERLDPGQTVKRSIKQLHEAVEGAFDPLGMAAPIIHAQLAWAAHPQELADRLLRLSSDLWRLQWHSWNRVLGLDSED
PVRPNPDDARFADPVWTESPTWDLTKEWYLALTHHVQDMLYDTPGLTGKERRRAAFWWRKWLNAVAPTNFFWTNPVAVRK
AWETKGESLVRGFGNFLDDVRAGEVRMTRPDDFEVGKNLATTPGAVVFRNDLLEVIHYQPTRPQVHRDPVVIVTPWINKF
YILDLVPKKSLIRYLLDQGLDVFITSWKNPDASMRDVTFDDYVTRGVHAAVETARGLAGAEKVHAVGYCIGGTALSMYMA
WANRHFPAEAVPVADWTVFTTLVDFQKPGDIEVFIDESSIRYLTGNMARTGYLEGKEMASAFRLLRSNSLIWHYVVHGWL
YGETPASFDVLYWNMDATRMPYAMHSWYLRELYLHNRLIQKDALVVAGEPLDLAAISQPLYAVAAEDDHIAPWGQTFRIN
NFVTGPKRYVLSSSGHILGIVNPVVQPPKRNYRAAEAHRSESAEAWLDRAERHEGSWWEDWMQWLKPRAGELVDARPVAN
AAFPALAPAPGTYVLEK

Sequences:

>Translated_577_residues
MNKSERLDPGQTVKRSIKQLHEAVEGAFDPLGMAAPIIHAQLAWAAHPQELADRLLRLSSDLWRLQWHSWNRVLGLDSED
PVRPNPDDARFADPVWTESPTWDLTKEWYLALTHHVQDMLYDTPGLTGKERRRAAFWWRKWLNAVAPTNFFWTNPVAVRK
AWETKGESLVRGFGNFLDDVRAGEVRMTRPDDFEVGKNLATTPGAVVFRNDLLEVIHYQPTRPQVHRDPVVIVTPWINKF
YILDLVPKKSLIRYLLDQGLDVFITSWKNPDASMRDVTFDDYVTRGVHAAVETARGLAGAEKVHAVGYCIGGTALSMYMA
WANRHFPAEAVPVADWTVFTTLVDFQKPGDIEVFIDESSIRYLTGNMARTGYLEGKEMASAFRLLRSNSLIWHYVVHGWL
YGETPASFDVLYWNMDATRMPYAMHSWYLRELYLHNRLIQKDALVVAGEPLDLAAISQPLYAVAAEDDHIAPWGQTFRIN
NFVTGPKRYVLSSSGHILGIVNPVVQPPKRNYRAAEAHRSESAEAWLDRAERHEGSWWEDWMQWLKPRAGELVDARPVAN
AAFPALAPAPGTYVLEK
>Mature_577_residues
MNKSERLDPGQTVKRSIKQLHEAVEGAFDPLGMAAPIIHAQLAWAAHPQELADRLLRLSSDLWRLQWHSWNRVLGLDSED
PVRPNPDDARFADPVWTESPTWDLTKEWYLALTHHVQDMLYDTPGLTGKERRRAAFWWRKWLNAVAPTNFFWTNPVAVRK
AWETKGESLVRGFGNFLDDVRAGEVRMTRPDDFEVGKNLATTPGAVVFRNDLLEVIHYQPTRPQVHRDPVVIVTPWINKF
YILDLVPKKSLIRYLLDQGLDVFITSWKNPDASMRDVTFDDYVTRGVHAAVETARGLAGAEKVHAVGYCIGGTALSMYMA
WANRHFPAEAVPVADWTVFTTLVDFQKPGDIEVFIDESSIRYLTGNMARTGYLEGKEMASAFRLLRSNSLIWHYVVHGWL
YGETPASFDVLYWNMDATRMPYAMHSWYLRELYLHNRLIQKDALVVAGEPLDLAAISQPLYAVAAEDDHIAPWGQTFRIN
NFVTGPKRYVLSSSGHILGIVNPVVQPPKRNYRAAEAHRSESAEAWLDRAERHEGSWWEDWMQWLKPRAGELVDARPVAN
AAFPALAPAPGTYVLEK

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010963
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 65760; Mature: 65760

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSERLDPGQTVKRSIKQLHEAVEGAFDPLGMAAPIIHAQLAWAAHPQELADRLLRLSS
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
DLWRLQWHSWNRVLGLDSEDPVRPNPDDARFADPVWTESPTWDLTKEWYLALTHHVQDML
HHHEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
YDTPGLTGKERRRAAFWWRKWLNAVAPTNFFWTNPVAVRKAWETKGESLVRGFGNFLDDV
HCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHCCHHHHHHHHHHHHHHH
RAGEVRMTRPDDFEVGKNLATTPGAVVFRNDLLEVIHYQPTRPQVHRDPVVIVTPWINKF
CCCCEEECCCCCHHHCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEE
YILDLVPKKSLIRYLLDQGLDVFITSWKNPDASMRDVTFDDYVTRGVHAAVETARGLAGA
EEEEECCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCH
EKVHAVGYCIGGTALSMYMAWANRHFPAEAVPVADWTVFTTLVDFQKPGDIEVFIDESSI
HHHHHEEEEHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCE
RYLTGNMARTGYLEGKEMASAFRLLRSNSLIWHYVVHGWLYGETPASFDVLYWNMDATRM
EEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEHHEEECCCCCCEEEEEEECCCCCC
PYAMHSWYLRELYLHNRLIQKDALVVAGEPLDLAAISQPLYAVAAEDDHIAPWGQTFRIN
CHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHCCCEEEEECCCCCCCCCCCEEEEC
NFVTGPKRYVLSSSGHILGIVNPVVQPPKRNYRAAEAHRSESAEAWLDRAERHEGSWWED
CCCCCCCCEEECCCCCEEEEECCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHCCCCCHHH
WMQWLKPRAGELVDARPVANAAFPALAPAPGTYVLEK
HHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCEEECC
>Mature Secondary Structure
MNKSERLDPGQTVKRSIKQLHEAVEGAFDPLGMAAPIIHAQLAWAAHPQELADRLLRLSS
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
DLWRLQWHSWNRVLGLDSEDPVRPNPDDARFADPVWTESPTWDLTKEWYLALTHHVQDML
HHHEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
YDTPGLTGKERRRAAFWWRKWLNAVAPTNFFWTNPVAVRKAWETKGESLVRGFGNFLDDV
HCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHCCHHHHHHHHHHHHHHH
RAGEVRMTRPDDFEVGKNLATTPGAVVFRNDLLEVIHYQPTRPQVHRDPVVIVTPWINKF
CCCCEEECCCCCHHHCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEE
YILDLVPKKSLIRYLLDQGLDVFITSWKNPDASMRDVTFDDYVTRGVHAAVETARGLAGA
EEEEECCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCH
EKVHAVGYCIGGTALSMYMAWANRHFPAEAVPVADWTVFTTLVDFQKPGDIEVFIDESSI
HHHHHEEEEHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCE
RYLTGNMARTGYLEGKEMASAFRLLRSNSLIWHYVVHGWLYGETPASFDVLYWNMDATRM
EEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEHHEEECCCCCCEEEEEEECCCCCC
PYAMHSWYLRELYLHNRLIQKDALVVAGEPLDLAAISQPLYAVAAEDDHIAPWGQTFRIN
CHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHCCCEEEEECCCCCCCCCCCEEEEC
NFVTGPKRYVLSSSGHILGIVNPVVQPPKRNYRAAEAHRSESAEAWLDRAERHEGSWWED
CCCCCCCCEEECCCCCEEEEECCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHCCCCCHHH
WMQWLKPRAGELVDARPVANAAFPALAPAPGTYVLEK
HHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748438 [H]