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The map label for this gene is fliA [H]

Identifier: 119897398

GI number: 119897398

Start: 1192166

End: 1192936

Strand: Direct

Name: fliA [H]

Synonym: azo1107

Alternate gene names: 119897398

Gene position: 1192166-1192936 (Clockwise)

Preceding gene: 119897397

Following gene: 119897399

Centisome position: 27.24

GC content: 68.61

Gene sequence:

>771_bases
ATGTACGACGCAGAGGGCAATCTCGACAAGGATCACCTCGTTGTAGCCTACGCCCCGCTGGTCAAGCGCATCGCTTTCCA
GTTGATGGCGAAGCTGCCGGCGAGCGTGGAGGTCGAGGATCTGATCCAGAACGGCATGATGGGCCTGCTCGACGCCATCG
GCCGCTACGAGGAAGGGCTGGGCGCCCAGTTCGAAACCTACGCGGTGCAGCGGGTGCGCGGCGCCATGCTCGACGGCCTG
CGCGAGAACGACTGGCTGCCGCGCAGCCTGCGGCGCGACATGCGGCGCATCGAATCCGCCATCCACGTGCTCGAACAGCA
GCATGGCCGCCCGCCGAGCGAAACCGAGCTGGCCGAGTCGCTCGGCGTGCCGCTGCCTGAGTACCAGCGCATGCTGCAGG
ACGCGCGCGGTCACCAGTTGGTGTATTTCGAGGATTTCACCGATGGCGAGGGCGAGGACTTCCTCGACCGCCACCTCGGC
GCGCACGAATCGAACCCGGCCGAGCTGCTGGAGGACGCCGACATGCGCGCGAACCTGATCCGCGCGATCGACGACCTGCC
AGAGCGCGAGAAGCTGGTGATGGCGCTGTACTACGAAGAAGAACTGAACCTGCGCGAGATCGGCGAGGTGCTGGGCGTCA
CGGAGTCGCGCGTGTGCCAGCTCCACAGCCAGGCGGTGGCCCGTCTGCGCGCCCGCGTCATGGGCGCCCGCGCCCCGGTC
AGCGGCGGTGCCCGTCGCGGCCGCCCGCCCAAACGGCCGCCGGAGGCCTGA

Upstream 100 bases:

>100_bases
GGCTGGAAATGGGGGCGGGGCTGCGGGAAAATGGCCTCCCCGCAATAGCGACGACGGCGAGCCCGAGCGGCCCCGGCGCA
AGAAGATACAGGCGACCTGG

Downstream 100 bases:

>100_bases
GCCCTTCCACGCCCTGCCGCCCGGCGGTCCGGTCTCTCCGGCCGGGCACCAACCGGACTGAACCCGCATGGACAGCATCA
GCCTCGTCGGACTCACCCTC

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-27; Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MYDAEGNLDKDHLVVAYAPLVKRIAFQLMAKLPASVEVEDLIQNGMMGLLDAIGRYEEGLGAQFETYAVQRVRGAMLDGL
RENDWLPRSLRRDMRRIESAIHVLEQQHGRPPSETELAESLGVPLPEYQRMLQDARGHQLVYFEDFTDGEGEDFLDRHLG
AHESNPAELLEDADMRANLIRAIDDLPEREKLVMALYYEEELNLREIGEVLGVTESRVCQLHSQAVARLRARVMGARAPV
SGGARRGRPPKRPPEA

Sequences:

>Translated_256_residues
MYDAEGNLDKDHLVVAYAPLVKRIAFQLMAKLPASVEVEDLIQNGMMGLLDAIGRYEEGLGAQFETYAVQRVRGAMLDGL
RENDWLPRSLRRDMRRIESAIHVLEQQHGRPPSETELAESLGVPLPEYQRMLQDARGHQLVYFEDFTDGEGEDFLDRHLG
AHESNPAELLEDADMRANLIRAIDDLPEREKLVMALYYEEELNLREIGEVLGVTESRVCQLHSQAVARLRARVMGARAPV
SGGARRGRPPKRPPEA
>Mature_256_residues
MYDAEGNLDKDHLVVAYAPLVKRIAFQLMAKLPASVEVEDLIQNGMMGLLDAIGRYEEGLGAQFETYAVQRVRGAMLDGL
RENDWLPRSLRRDMRRIESAIHVLEQQHGRPPSETELAESLGVPLPEYQRMLQDARGHQLVYFEDFTDGEGEDFLDRHLG
AHESNPAELLEDADMRANLIRAIDDLPEREKLVMALYYEEELNLREIGEVLGVTESRVCQLHSQAVARLRARVMGARAPV
SGGARRGRPPKRPPEA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for class 3 flagellar operons [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=236, Percent_Identity=50, Blast_Score=226, Evalue=1e-60,
Organism=Escherichia coli, GI1789098, Length=209, Percent_Identity=25.3588516746411, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1789448, Length=213, Percent_Identity=29.1079812206573, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 28893; Mature: 28893

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDAEGNLDKDHLVVAYAPLVKRIAFQLMAKLPASVEVEDLIQNGMMGLLDAIGRYEEGL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GAQFETYAVQRVRGAMLDGLRENDWLPRSLRRDMRRIESAIHVLEQQHGRPPSETELAES
CCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LGVPLPEYQRMLQDARGHQLVYFEDFTDGEGEDFLDRHLGAHESNPAELLEDADMRANLI
HCCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
RAIDDLPEREKLVMALYYEEELNLREIGEVLGVTESRVCQLHSQAVARLRARVMGARAPV
HHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
SGGARRGRPPKRPPEA
CCCCCCCCCCCCCCCC
>Mature Secondary Structure
MYDAEGNLDKDHLVVAYAPLVKRIAFQLMAKLPASVEVEDLIQNGMMGLLDAIGRYEEGL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GAQFETYAVQRVRGAMLDGLRENDWLPRSLRRDMRRIESAIHVLEQQHGRPPSETELAES
CCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LGVPLPEYQRMLQDARGHQLVYFEDFTDGEGEDFLDRHLGAHESNPAELLEDADMRANLI
HCCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
RAIDDLPEREKLVMALYYEEELNLREIGEVLGVTESRVCQLHSQAVARLRARVMGARAPV
HHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
SGGARRGRPPKRPPEA
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]