Click here to switch to the map view.

The map label for this gene is thrS

Identifier: 119897370

GI number: 119897370

Start: 1162660

End: 1164576

Strand: Direct

Name: thrS

Synonym: azo1079

Alternate gene names: 119897370

Gene position: 1162660-1164576 (Clockwise)

Preceding gene: 119897369

Following gene: 119897371

Centisome position: 26.57

GC content: 61.45

Gene sequence:

>1917_bases
ATGCCGAATATCAAGCTTCCAGATGGTTCTGTCCGCAGCTTCGATCATCCCGTCACGGTCGCGGAGGTCGCTGCGTCCAT
TGGCTCCGGCCTCGCCAAGGCCGCGTTGGCGGGTCGGGTGGATGGAAAGGCGGTCGATCTGTCGTATTGCATCGAACGCG
ACAGCGAGCTGGCCATCCTCACCGACAAGAGTGCGGAGGGCGTGGATGTCATCCGCCATTCCACTGCGCACCTGCTGGCG
CATGCGGTCAAGGAGTTGTTTCCGGAAGCGCAGGTCACCATCGGGCCGGTGATCGAAAACGGGTTCTACTACGACTTTTC
GTACAAGCGCCCGTTTACGCCGGAGGACCTCGAGGCGATCGAAAAGCGCATGGCCGAACTCGTCAAGCGCGAACTCCCGG
TGGAGCGGGAGGTGTGGCCGCGCGACAAGGCGGTGGCGTTTTTCAAGTCGATCGGGGAGCACTACAAGGCCGAGATCATC
GCCTCGATCCCCGCGAACGAAGACGTGTCGCTGTATCGCCAGGGTGAGTTCGTCGATCTGTGTCGCGGGCCTCACGTGCC
TTCGACCGGCAGGCTCAAGGTCTTCAAGTTGACCAAGCTTGCGGGCGCGTATTGGCGCGGTGACGCCAAGAACGAGATGC
TGCAGCGGGTGTATGGAACCGCGTGGGCGAAAAAAGAGGACCTCGAGTCCTATCTCCATATGCTGGAGGAGGCCGAGAAG
CGGGATCACCGCAAACTGGGACGCCAACTGGATCTGTTCCACATCCAGGAAGAGGCCCCCGGTATGGTGTTCTGGCACGC
GAAGGGCTGGACGCTGTGGCAGCAGGTCGAGCAGTACCTGCGCGGGACGATTTCCCGTCATGGCTACCAGGAGGTTCGCA
CGCCGCAGATCGTAGACCGCTCGCTGTGGGAGAAGTCGGGGCATTGGGGCATGTATTCCGACCTGATGTTCACCACGCAG
TCCGAGAAGCACGACTACGCCGTGAAGCCGATGAACTGCCCGTGCCACATCCAGATCTTCAACCAAGGGCTGAAGAGCTA
TCGCGACCTGCCGCTGCGGATGGCGGAGTTCGGCTCCTGTCATCGCAACGAGCCGTCCGGTTCCTTGCACGGCATCATGC
GCGTGCGCAACTTCGTCCAGGACGATGCGCACATCTTCTGCACCGATGAACAGGTGCAGGAAGAAGCGGCGGCATTCATT
GCGCTGTTGCAGAAGGTCTATGCGGACTTCGGGTTCCGCGACATCCAGATCAAGCTCTCGACTCGCCCCGAGAAGCGCGT
CGGCGCCGACGAGCAGTGGGATGCGGCCGAGGCGGCGCTGGCTGCGGCGCTGAAAGCGCAGGGGCTGGAATACGAACTGC
AGCCCGGGGAGGGCGCTTTCTACGGCCCCAAGATCGAGTTTTCACTGAAGGACTGTCTGAACCGCGTCTGGCAGTGCGGC
ACGCTCCAGCTCGATTTCAACCTTCCGGTACGTCTCGGGGCGGAGTATGTGGCCGAGGACAACACCAAGAAGTTCCCGGT
GATGTTGCACCGCGCCATCGTGGGCTCGCTGGAGCGCTTCATCGGCATCCTGATCGAACACTACGCGGGCGCGATGCCGC
TCTGGCTGGCGCCGGTTCACGCCGTGGTCATGAACATCTCCGAAGGGCAGGCGGACTACGCCACGGAAGTGGTCAATCGG
CTTCGCGAGGCCGGATTCCGGGTGGAGGCTGATTTGCGTAACGAAAAGATTAACTATAAAATCCGCGAACATAGCGTGCA
CAAGCTACCTTACCAGCTCGTGGTAGGCGAAAAAGAAAAGGCTGCGGGTGTAGTGGCGGTACGCGTCCGGGGTGGCCAGG
ATCTTGGCCAACTGTCCCTCGATAAGTTGATCGAACGCTGGAACGGCGAACTGGCTTCGCGCTCCGGCCCGGTCTGA

Upstream 100 bases:

>100_bases
GCGGTTAGACCGCACTTTTTTTTCGTCCACGTTTTTCGTGCGATCGACGCGAGCAGTGCGCGTCCCGCCTGCACTGTTCT
CCTTCGAGGGTCCAGAGATC

Downstream 100 bases:

>100_bases
TTTTGGTTTTCGTGGAGAACTGAACCATCGCTCAAGACAACAAGAAGCAGCGCGTCAATGGGGAGATCAACGCAACGGAA
GTGCGTCTGGTCGGTGAAGA

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 638; Mature: 637

Protein sequence:

>638_residues
MPNIKLPDGSVRSFDHPVTVAEVAASIGSGLAKAALAGRVDGKAVDLSYCIERDSELAILTDKSAEGVDVIRHSTAHLLA
HAVKELFPEAQVTIGPVIENGFYYDFSYKRPFTPEDLEAIEKRMAELVKRELPVEREVWPRDKAVAFFKSIGEHYKAEII
ASIPANEDVSLYRQGEFVDLCRGPHVPSTGRLKVFKLTKLAGAYWRGDAKNEMLQRVYGTAWAKKEDLESYLHMLEEAEK
RDHRKLGRQLDLFHIQEEAPGMVFWHAKGWTLWQQVEQYLRGTISRHGYQEVRTPQIVDRSLWEKSGHWGMYSDLMFTTQ
SEKHDYAVKPMNCPCHIQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGIMRVRNFVQDDAHIFCTDEQVQEEAAAFI
ALLQKVYADFGFRDIQIKLSTRPEKRVGADEQWDAAEAALAAALKAQGLEYELQPGEGAFYGPKIEFSLKDCLNRVWQCG
TLQLDFNLPVRLGAEYVAEDNTKKFPVMLHRAIVGSLERFIGILIEHYAGAMPLWLAPVHAVVMNISEGQADYATEVVNR
LREAGFRVEADLRNEKINYKIREHSVHKLPYQLVVGEKEKAAGVVAVRVRGGQDLGQLSLDKLIERWNGELASRSGPV

Sequences:

>Translated_638_residues
MPNIKLPDGSVRSFDHPVTVAEVAASIGSGLAKAALAGRVDGKAVDLSYCIERDSELAILTDKSAEGVDVIRHSTAHLLA
HAVKELFPEAQVTIGPVIENGFYYDFSYKRPFTPEDLEAIEKRMAELVKRELPVEREVWPRDKAVAFFKSIGEHYKAEII
ASIPANEDVSLYRQGEFVDLCRGPHVPSTGRLKVFKLTKLAGAYWRGDAKNEMLQRVYGTAWAKKEDLESYLHMLEEAEK
RDHRKLGRQLDLFHIQEEAPGMVFWHAKGWTLWQQVEQYLRGTISRHGYQEVRTPQIVDRSLWEKSGHWGMYSDLMFTTQ
SEKHDYAVKPMNCPCHIQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGIMRVRNFVQDDAHIFCTDEQVQEEAAAFI
ALLQKVYADFGFRDIQIKLSTRPEKRVGADEQWDAAEAALAAALKAQGLEYELQPGEGAFYGPKIEFSLKDCLNRVWQCG
TLQLDFNLPVRLGAEYVAEDNTKKFPVMLHRAIVGSLERFIGILIEHYAGAMPLWLAPVHAVVMNISEGQADYATEVVNR
LREAGFRVEADLRNEKINYKIREHSVHKLPYQLVVGEKEKAAGVVAVRVRGGQDLGQLSLDKLIERWNGELASRSGPV
>Mature_637_residues
PNIKLPDGSVRSFDHPVTVAEVAASIGSGLAKAALAGRVDGKAVDLSYCIERDSELAILTDKSAEGVDVIRHSTAHLLAH
AVKELFPEAQVTIGPVIENGFYYDFSYKRPFTPEDLEAIEKRMAELVKRELPVEREVWPRDKAVAFFKSIGEHYKAEIIA
SIPANEDVSLYRQGEFVDLCRGPHVPSTGRLKVFKLTKLAGAYWRGDAKNEMLQRVYGTAWAKKEDLESYLHMLEEAEKR
DHRKLGRQLDLFHIQEEAPGMVFWHAKGWTLWQQVEQYLRGTISRHGYQEVRTPQIVDRSLWEKSGHWGMYSDLMFTTQS
EKHDYAVKPMNCPCHIQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGIMRVRNFVQDDAHIFCTDEQVQEEAAAFIA
LLQKVYADFGFRDIQIKLSTRPEKRVGADEQWDAAEAALAAALKAQGLEYELQPGEGAFYGPKIEFSLKDCLNRVWQCGT
LQLDFNLPVRLGAEYVAEDNTKKFPVMLHRAIVGSLERFIGILIEHYAGAMPLWLAPVHAVVMNISEGQADYATEVVNRL
REAGFRVEADLRNEKINYKIREHSVHKLPYQLVVGEKEKAAGVVAVRVRGGQDLGQLSLDKLIERWNGELASRSGPV

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38570111, Length=637, Percent_Identity=39.0894819466248, Blast_Score=473, Evalue=1e-133,
Organism=Homo sapiens, GI38202255, Length=627, Percent_Identity=39.7129186602871, Blast_Score=470, Evalue=1e-132,
Organism=Homo sapiens, GI20070344, Length=645, Percent_Identity=37.5193798449612, Blast_Score=411, Evalue=1e-114,
Organism=Escherichia coli, GI1788013, Length=636, Percent_Identity=58.9622641509434, Blast_Score=806, Evalue=0.0,
Organism=Escherichia coli, GI1786392, Length=167, Percent_Identity=29.3413173652695, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71984184, Length=628, Percent_Identity=40.2866242038217, Blast_Score=485, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI71984192, Length=628, Percent_Identity=40.2866242038217, Blast_Score=485, Evalue=1e-137,
Organism=Saccharomyces cerevisiae, GI6322112, Length=627, Percent_Identity=39.2344497607655, Blast_Score=463, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6322654, Length=413, Percent_Identity=36.0774818401937, Blast_Score=278, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24583839, Length=626, Percent_Identity=40.5750798722045, Blast_Score=494, Evalue=1e-140,
Organism=Drosophila melanogaster, GI24583842, Length=626, Percent_Identity=40.5750798722045, Blast_Score=494, Evalue=1e-140,
Organism=Drosophila melanogaster, GI28574141, Length=626, Percent_Identity=40.5750798722045, Blast_Score=494, Evalue=1e-140,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_AZOSB (A1K4E1)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932583.1
- ProteinModelPortal:   A1K4E1
- SMR:   A1K4E1
- STRING:   A1K4E1
- GeneID:   4610025
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo1079
- eggNOG:   COG0441
- HOGENOM:   HBG352811
- OMA:   TAKDPEC
- PhylomeDB:   A1K4E1
- ProtClustDB:   PRK00413
- BioCyc:   ASP62928:AZO1079-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF81271 TGS-like; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 72144; Mature: 72013

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNIKLPDGSVRSFDHPVTVAEVAASIGSGLAKAALAGRVDGKAVDLSYCIERDSELAIL
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHCCCCCEEEE
TDKSAEGVDVIRHSTAHLLAHAVKELFPEAQVTIGPVIENGFYYDFSYKRPFTPEDLEAI
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCCCHHHHHHH
EKRMAELVKRELPVEREVWPRDKAVAFFKSIGEHYKAEIIASIPANEDVSLYRQGEFVDL
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCEEEECCCCHHH
CRGPHVPSTGRLKVFKLTKLAGAYWRGDAKNEMLQRVYGTAWAKKEDLESYLHMLEEAEK
HCCCCCCCCCCEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RDHRKLGRQLDLFHIQEEAPGMVFWHAKGWTLWQQVEQYLRGTISRHGYQEVRTPQIVDR
HHHHHHCCEECEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHCCHHHHHH
SLWEKSGHWGMYSDLMFTTQSEKHDYAVKPMNCPCHIQIFNQGLKSYRDLPLRMAEFGSC
HHHHCCCCCCCHHHEEEEECCCCCCCEECCCCCCEEEHHHHHHHHHHHCCCHHHHHHCCC
HRNEPSGSLHGIMRVRNFVQDDAHIFCTDEQVQEEAAAFIALLQKVYADFGFRDIQIKLS
CCCCCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
TRPEKRVGADEQWDAAEAALAAALKAQGLEYELQPGEGAFYGPKIEFSLKDCLNRVWQCG
CCCHHHCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCEECCEEEEEHHHHHHHHHCCC
TLQLDFNLPVRLGAEYVAEDNTKKFPVMLHRAIVGSLERFIGILIEHYAGAMPLWLAPVH
EEEEECCCCHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
AVVMNISEGQADYATEVVNRLREAGFRVEADLRNEKINYKIREHSVHKLPYQLVVGEKEK
HHHHCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCEEECCCCHHHCCEEEEECCCCC
AAGVVAVRVRGGQDLGQLSLDKLIERWNGELASRSGPV
CCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PNIKLPDGSVRSFDHPVTVAEVAASIGSGLAKAALAGRVDGKAVDLSYCIERDSELAIL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHCCCCCEEEE
TDKSAEGVDVIRHSTAHLLAHAVKELFPEAQVTIGPVIENGFYYDFSYKRPFTPEDLEAI
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCCCHHHHHHH
EKRMAELVKRELPVEREVWPRDKAVAFFKSIGEHYKAEIIASIPANEDVSLYRQGEFVDL
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCEEEECCCCHHH
CRGPHVPSTGRLKVFKLTKLAGAYWRGDAKNEMLQRVYGTAWAKKEDLESYLHMLEEAEK
HCCCCCCCCCCEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RDHRKLGRQLDLFHIQEEAPGMVFWHAKGWTLWQQVEQYLRGTISRHGYQEVRTPQIVDR
HHHHHHCCEECEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHCCHHHHHH
SLWEKSGHWGMYSDLMFTTQSEKHDYAVKPMNCPCHIQIFNQGLKSYRDLPLRMAEFGSC
HHHHCCCCCCCHHHEEEEECCCCCCCEECCCCCCEEEHHHHHHHHHHHCCCHHHHHHCCC
HRNEPSGSLHGIMRVRNFVQDDAHIFCTDEQVQEEAAAFIALLQKVYADFGFRDIQIKLS
CCCCCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
TRPEKRVGADEQWDAAEAALAAALKAQGLEYELQPGEGAFYGPKIEFSLKDCLNRVWQCG
CCCHHHCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCEECCEEEEEHHHHHHHHHCCC
TLQLDFNLPVRLGAEYVAEDNTKKFPVMLHRAIVGSLERFIGILIEHYAGAMPLWLAPVH
EEEEECCCCHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
AVVMNISEGQADYATEVVNRLREAGFRVEADLRNEKINYKIREHSVHKLPYQLVVGEKEK
HHHHCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCEEECCCCHHHCCEEEEECCCCC
AAGVVAVRVRGGQDLGQLSLDKLIERWNGELASRSGPV
CCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA