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The map label for this gene is folC [H]

Identifier: 119897341

GI number: 119897341

Start: 1129592

End: 1130890

Strand: Direct

Name: folC [H]

Synonym: azo1050

Alternate gene names: 119897341

Gene position: 1129592-1130890 (Clockwise)

Preceding gene: 119897340

Following gene: 119897342

Centisome position: 25.81

GC content: 68.98

Gene sequence:

>1299_bases
ATGTCTTTGCCGCACACCCTGGACGGCTGGCTGGAACTGCTCGAGCGCCGTCACGCCCAGGCCATTCAGCTGGGGCTGGA
GCGCGTGCGCACGGTGCGCGATGCGCTGGGCCCCGGTCCGGAGACCGTGGTGATCACGGTCGGCGGTACCAATGGCAAGG
GCTCGACCTGCGCGATGCTCGAGGCGATCCTGCGCGCCGAGGGTTACCGTGTCGGCTGCTACACCTCGCCCCATCTGCTG
CGCTACAACGAGCGCGTGCGGGTCGATGGCCAGGACGTGGACGACGCGCGCCTGGTCGCGGGCTTCGAAGCGGTGGAGCG
CGCCCGTGGCGACGTTCCGCTGACGTATTTCGAGCACGGCACGCTGGCGGCGTGGGAGGTGTTCCGCGCGGCCGCGCCGG
ACGTCGTGATCCTCGAGGTGGGATTGGGCGGCCGTCTGGACGCGGTGAACGTGTTCGAGCCCGACTGTGCGATCGTCACC
AGTGTGGCGATGGACCACATGGATTATCTCGGCGACACGCGCGAGGCCATCGGCTACGAGAAGGCGGGCATCTTCCGCAG
CGGCCGTCCCGCGGTCTGCGGTGATCCCCAACCGCCCGCTTCGCTGCGCGAGCACGCCGAGGCGATTGCCGCGCAACTGT
GGATCAGCGGGCGCGATTTCGGCTTCGGCGGTGACCAGCAGCAGTGGGGCTACTGGCGTTATCCGGCCCCCGCCACCCGC
GGTGCGCTGGTCAAGCGGGGCGGCCTGGCCTATCCCGCGCTGCGCGGCGCCAACCAGTTGCTCAACGCGGCCGCGGTGTT
TACCGCGCTGGATACGCTGCGCGACCGCATTCCGGTGTCGATGCAGGCGATCCGCCAGGGCTTGATGCTGGTCGAGGTGC
CCGGACGCTTCCAGGTGTTGCCGGGCAAGCCCGCGGTGGTGCTGGACGTGGCCCACAATCCGCAGGCCGCCGGCGTGCTC
GCCGAGAACCTCGGCGGCATGGGGTTCTACCCCGAAACCTGGGCGGTACTCGGCATGCTGGCGGACAAGGACGTGGAAGG
TGTGGTCGAACGGATGCGGCATCGGGTCGACCACTGGTTGCTCGCCAGCCTGCCGGGCGCGCGCGGCCTTGACGCCGAAA
CGCTCGCCGCGCGCTTGCATGCCGCGGGAGTGGCGGGGGATGTACGCTGCTTTCCCGATCCGCAAGTCGCATTTTCCGCA
GCGCGGAAAAGCGCTGCCGAGGGTGATAGAATCGTCGCCTTTGGTTCCTTCCTGACCGTGGCGAGTGTATTGGCAGCGAT
TCGCGCCGAGCGACACTGA

Upstream 100 bases:

>100_bases
ATGATCGTGGACCGTCGCGAACTGCGCGAGCGGCTTGCCGCGCTGCTGACGCTGATGACCCGCCAGCCGGCGGTCGGCAA
CTGAGGCAGCCCTGAGCGAC

Downstream 100 bases:

>100_bases
GGGGTTTCCGGGTGAGCGACGCAGACAACCTTGAAATCAAGAAGCGTGCGCGCCGGCGCCTGGTGGGTGCGGCGGCGCTG
GCGCTTACGGCCGCCATCGT

Product: dihydrofolate synthase/tetrahydrofolylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MSLPHTLDGWLELLERRHAQAIQLGLERVRTVRDALGPGPETVVITVGGTNGKGSTCAMLEAILRAEGYRVGCYTSPHLL
RYNERVRVDGQDVDDARLVAGFEAVERARGDVPLTYFEHGTLAAWEVFRAAAPDVVILEVGLGGRLDAVNVFEPDCAIVT
SVAMDHMDYLGDTREAIGYEKAGIFRSGRPAVCGDPQPPASLREHAEAIAAQLWISGRDFGFGGDQQQWGYWRYPAPATR
GALVKRGGLAYPALRGANQLLNAAAVFTALDTLRDRIPVSMQAIRQGLMLVEVPGRFQVLPGKPAVVLDVAHNPQAAGVL
AENLGGMGFYPETWAVLGMLADKDVEGVVERMRHRVDHWLLASLPGARGLDAETLAARLHAAGVAGDVRCFPDPQVAFSA
ARKSAAEGDRIVAFGSFLTVASVLAAIRAERH

Sequences:

>Translated_432_residues
MSLPHTLDGWLELLERRHAQAIQLGLERVRTVRDALGPGPETVVITVGGTNGKGSTCAMLEAILRAEGYRVGCYTSPHLL
RYNERVRVDGQDVDDARLVAGFEAVERARGDVPLTYFEHGTLAAWEVFRAAAPDVVILEVGLGGRLDAVNVFEPDCAIVT
SVAMDHMDYLGDTREAIGYEKAGIFRSGRPAVCGDPQPPASLREHAEAIAAQLWISGRDFGFGGDQQQWGYWRYPAPATR
GALVKRGGLAYPALRGANQLLNAAAVFTALDTLRDRIPVSMQAIRQGLMLVEVPGRFQVLPGKPAVVLDVAHNPQAAGVL
AENLGGMGFYPETWAVLGMLADKDVEGVVERMRHRVDHWLLASLPGARGLDAETLAARLHAAGVAGDVRCFPDPQVAFSA
ARKSAAEGDRIVAFGSFLTVASVLAAIRAERH
>Mature_431_residues
SLPHTLDGWLELLERRHAQAIQLGLERVRTVRDALGPGPETVVITVGGTNGKGSTCAMLEAILRAEGYRVGCYTSPHLLR
YNERVRVDGQDVDDARLVAGFEAVERARGDVPLTYFEHGTLAAWEVFRAAAPDVVILEVGLGGRLDAVNVFEPDCAIVTS
VAMDHMDYLGDTREAIGYEKAGIFRSGRPAVCGDPQPPASLREHAEAIAAQLWISGRDFGFGGDQQQWGYWRYPAPATRG
ALVKRGGLAYPALRGANQLLNAAAVFTALDTLRDRIPVSMQAIRQGLMLVEVPGRFQVLPGKPAVVLDVAHNPQAAGVLA
ENLGGMGFYPETWAVLGMLADKDVEGVVERMRHRVDHWLLASLPGARGLDAETLAARLHAAGVAGDVRCFPDPQVAFSAA
RKSAAEGDRIVAFGSFLTVASVLAAIRAERH

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=182, Percent_Identity=38.4615384615385, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI66932990, Length=182, Percent_Identity=38.4615384615385, Blast_Score=122, Evalue=7e-28,
Organism=Escherichia coli, GI1788654, Length=426, Percent_Identity=43.4272300469484, Blast_Score=311, Evalue=4e-86,
Organism=Caenorhabditis elegans, GI17553150, Length=422, Percent_Identity=27.9620853080569, Blast_Score=130, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI71984923, Length=422, Percent_Identity=27.9620853080569, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17553148, Length=422, Percent_Identity=27.9620853080569, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6323760, Length=434, Percent_Identity=27.4193548387097, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6324815, Length=185, Percent_Identity=34.5945945945946, Blast_Score=106, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24641571, Length=180, Percent_Identity=36.6666666666667, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24581568, Length=146, Percent_Identity=34.9315068493151, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 46466; Mature: 46334

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPHTLDGWLELLERRHAQAIQLGLERVRTVRDALGPGPETVVITVGGTNGKGSTCAML
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHH
EAILRAEGYRVGCYTSPHLLRYNERVRVDGQDVDDARLVAGFEAVERARGDVPLTYFEHG
HHHHHHCCEEEEEECCCHHEECCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEECCC
TLAAWEVFRAAAPDVVILEVGLGGRLDAVNVFEPDCAIVTSVAMDHMDYLGDTREAIGYE
CHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHHCCH
KAGIFRSGRPAVCGDPQPPASLREHAEAIAAQLWISGRDFGFGGDQQQWGYWRYPAPATR
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCC
GALVKRGGLAYPALRGANQLLNAAAVFTALDTLRDRIPVSMQAIRQGLMLVEVPGRFQVL
CHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEECCCCEEEC
PGKPAVVLDVAHNPQAAGVLAENLGGMGFYPETWAVLGMLADKDVEGVVERMRHRVDHWL
CCCCEEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LASLPGARGLDAETLAARLHAAGVAGDVRCFPDPQVAFSAARKSAAEGDRIVAFGSFLTV
HHCCCCCCCCCHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHCCCCEEEEEHHHHHH
ASVLAAIRAERH
HHHHHHHHHCCC
>Mature Secondary Structure 
SLPHTLDGWLELLERRHAQAIQLGLERVRTVRDALGPGPETVVITVGGTNGKGSTCAML
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHH
EAILRAEGYRVGCYTSPHLLRYNERVRVDGQDVDDARLVAGFEAVERARGDVPLTYFEHG
HHHHHHCCEEEEEECCCHHEECCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEECCC
TLAAWEVFRAAAPDVVILEVGLGGRLDAVNVFEPDCAIVTSVAMDHMDYLGDTREAIGYE
CHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHHCCH
KAGIFRSGRPAVCGDPQPPASLREHAEAIAAQLWISGRDFGFGGDQQQWGYWRYPAPATR
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCC
GALVKRGGLAYPALRGANQLLNAAAVFTALDTLRDRIPVSMQAIRQGLMLVEVPGRFQVL
CHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEECCCCEEEC
PGKPAVVLDVAHNPQAAGVLAENLGGMGFYPETWAVLGMLADKDVEGVVERMRHRVDHWL
CCCCEEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LASLPGARGLDAETLAARLHAAGVAGDVRCFPDPQVAFSAARKSAAEGDRIVAFGSFLTV
HHCCCCCCCCCHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHCCCCEEEEEHHHHHH
ASVLAAIRAERH
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]