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The map label for this gene is truA [H]

Identifier: 119897336

GI number: 119897336

Start: 1125226

End: 1126023

Strand: Direct

Name: truA [H]

Synonym: azo1045

Alternate gene names: 119897336

Gene position: 1125226-1126023 (Clockwise)

Preceding gene: 119897335

Following gene: 119897337

Centisome position: 25.71

GC content: 68.3

Gene sequence:

>798_bases
GTGGTGAGACTGGCGCTGGGCGTGGAATACGCGGGCGATCGCTTCGAAGGCTGGCAAAGCCAGCGCCATGGGCGGACGGT
GCAGGATGCGCTCGAAGCGGCGCTGTCGCGGATCGCCGGCGAAGCGGTGAAGTTGCATTGCGCGGGGCGGACTGACACCG
GTGTGCATGCCACCGCCCAGGTGGCTCATTTCGACACCGCCGCGGTGCGTCCGCTGTCGGCGTGGATACGCGGCGTGAAC
ACCCATCTGCCGGCCGGCGTCGCGGTGCGTTGGGCAGTCGAAGTCGATGACGCTTTCCATGCCCGCTTCCTGGCCTACGA
GCGTCGCTACCGCTACGTGCTGTTCAATGCGCCGACGCGGCCGGCGCTGCTGGCCGGCAGAGTGGGCTGGTTTCACCTGC
CGCTGGACGAGGGGGCGATGGCGGAGGCGGCCCGTTGCCTCGTCGGCATGCAGGACTTCTCCGCATTTCGCGCCGCGGAG
TGCCAGGCCAAGACGCCGGTGCGCGACATGCGGGAGGTCCGGGTGCAGCGTAGCGGGGACTACCTGATCTTCGATTTCCG
CGCCGACGGCTTCCTGCATCACATGATCCGCAATCTGGTGGGGGCGCTGGTGTATGTGGGCAAGGGGCGATATCCGCCGG
GGTGGCTGGCCGAAGTGCTGGCGGGGCGGGACCGTTCGCGCGCTGCGCCGACCTTTGCACCCGACGGCCTTTATCTGTGC
GGCGTCAGCTATCCGGGCAACTGGTCGCTGCCTGACGATGGGCGTATCATCGCGCTGCCCCGGATTCCCCTCGTCTGA

Upstream 100 bases:

>100_bases
AGGAGGGCATGCTGTTGCAGCGCGAGCAGTGGCGCGCGCGTGATGTCGCGGTGCTGCTGACGGTGGTGCTCGGGTTGGGG
CTTTGGGTGAGCGGGGTAGT

Downstream 100 bases:

>100_bases
ATGTCCCGTACCCGTATCAAGATTTGTGGCCTGACCCGGCCGGATGACGTGCGCGCGGCCGTCGATGCGGGCGCGGACGC
GATCGGCTTCGTCTTCTATC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MVRLALGVEYAGDRFEGWQSQRHGRTVQDALEAALSRIAGEAVKLHCAGRTDTGVHATAQVAHFDTAAVRPLSAWIRGVN
THLPAGVAVRWAVEVDDAFHARFLAYERRYRYVLFNAPTRPALLAGRVGWFHLPLDEGAMAEAARCLVGMQDFSAFRAAE
CQAKTPVRDMREVRVQRSGDYLIFDFRADGFLHHMIRNLVGALVYVGKGRYPPGWLAEVLAGRDRSRAAPTFAPDGLYLC
GVSYPGNWSLPDDGRIIALPRIPLV

Sequences:

>Translated_265_residues
MVRLALGVEYAGDRFEGWQSQRHGRTVQDALEAALSRIAGEAVKLHCAGRTDTGVHATAQVAHFDTAAVRPLSAWIRGVN
THLPAGVAVRWAVEVDDAFHARFLAYERRYRYVLFNAPTRPALLAGRVGWFHLPLDEGAMAEAARCLVGMQDFSAFRAAE
CQAKTPVRDMREVRVQRSGDYLIFDFRADGFLHHMIRNLVGALVYVGKGRYPPGWLAEVLAGRDRSRAAPTFAPDGLYLC
GVSYPGNWSLPDDGRIIALPRIPLV
>Mature_265_residues
MVRLALGVEYAGDRFEGWQSQRHGRTVQDALEAALSRIAGEAVKLHCAGRTDTGVHATAQVAHFDTAAVRPLSAWIRGVN
THLPAGVAVRWAVEVDDAFHARFLAYERRYRYVLFNAPTRPALLAGRVGWFHLPLDEGAMAEAARCLVGMQDFSAFRAAE
CQAKTPVRDMREVRVQRSGDYLIFDFRADGFLHHMIRNLVGALVYVGKGRYPPGWLAEVLAGRDRSRAAPTFAPDGLYLC
GVSYPGNWSLPDDGRIIALPRIPLV

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=273, Percent_Identity=33.3333333333333, Blast_Score=95, Evalue=8e-20,
Organism=Escherichia coli, GI1788657, Length=249, Percent_Identity=49.7991967871486, Blast_Score=270, Evalue=8e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 29310; Mature: 29310

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRLALGVEYAGDRFEGWQSQRHGRTVQDALEAALSRIAGEAVKLHCAGRTDTGVHATAQ
CEEEEEECEECCCCHHCHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEE
VAHFDTAAVRPLSAWIRGVNTHLPAGVAVRWAVEVDDAFHARFLAYERRYRYVLFNAPTR
EECCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCHHHHHHHHHHCCEEEEEEECCCC
PALLAGRVGWFHLPLDEGAMAEAARCLVGMQDFSAFRAAECQAKTPVRDMREVRVQRSGD
CCEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
YLIFDFRADGFLHHMIRNLVGALVYVGKGRYPPGWLAEVLAGRDRSRAAPTFAPDGLYLC
EEEEEECCCHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEE
GVSYPGNWSLPDDGRIIALPRIPLV
EECCCCCCCCCCCCCEEEECCCCCC
>Mature Secondary Structure
MVRLALGVEYAGDRFEGWQSQRHGRTVQDALEAALSRIAGEAVKLHCAGRTDTGVHATAQ
CEEEEEECEECCCCHHCHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEE
VAHFDTAAVRPLSAWIRGVNTHLPAGVAVRWAVEVDDAFHARFLAYERRYRYVLFNAPTR
EECCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCHHHHHHHHHHCCEEEEEEECCCC
PALLAGRVGWFHLPLDEGAMAEAARCLVGMQDFSAFRAAECQAKTPVRDMREVRVQRSGD
CCEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
YLIFDFRADGFLHHMIRNLVGALVYVGKGRYPPGWLAEVLAGRDRSRAAPTFAPDGLYLC
EEEEEECCCHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEE
GVSYPGNWSLPDDGRIIALPRIPLV
EECCCCCCCCCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA