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The map label for this gene is rho [H]

Identifier: 119897320

GI number: 119897320

Start: 1106727

End: 1108178

Strand: Reverse

Name: rho [H]

Synonym: azo1029

Alternate gene names: 119897320

Gene position: 1108178-1106727 (Counterclockwise)

Preceding gene: 119897321

Following gene: 119897311

Centisome position: 25.32

GC content: 62.95

Gene sequence:

>1452_bases
TTGACAGCAACATCTAAGCCGGAGGCGACTCCGGCCCGTTCTCGCGGTGCTTCGCGGACCCGCCGCAGGGGTGCCCGCAA
CCGCGACGGTAATTCTCCCCAAGGCAACACCTCCGAACTCGACCTGCTCGATGCCGAGCTGGCGATGGAAGAGGGTTCTG
CTTCGTCCGCCGAATCCACGGCTGCTGCGGCCTTGCATCTGTCGGAGCTGAAAGCTCTGCATGTGAGCCAGCTGCTGGAA
ATGGCGGTTGCCAACGAAATCGAGGGTGCGAACCGGCTCCGCAAGCAGGAACTCGTCTTCGCGCTGCTGAAGAATCGTGC
GCGCAAGGGCGAGCCGATCTACGGCGACGGCGCGCTTGAAGTCCTGCCAGACGGCTTCGGCTTCCTGCGCTCGCCCGACA
CCTCGTACCTGGCGGGTACGGACGACATCTACGTGTCGCCCTCGCAGATCCGGCGTTTCAATCTGCACACCGGCGACACC
ATCGAGGGCGAGATCCGCACCCCGAAGGATGGCGAACGCTACTTTGCGCTGGTCAAGCTCGACAAGATCAACGGGCGACC
GCCGGAAGAGTGCAAGCACAAGATCCTGTTCGAGAACCTCACGCCGCTGCATCCGCAGGAGTGCCTGAAGCTCGAACGCG
AGATCCGCGGCGAAGAGAATGTCACCAGCCGCATCATCGACATGATCGCGCCGATCGGCAAAGGCCAGCGCGGCCTGCTC
GTCGCCCCGCCGAAGAGCGGCAAGACGGTGATGCTGCAGCACATCGCCCACGCGATCACGGCCAACCATCCGGACGTGGA
GTTGATCGTGCTGCTGATCGACGAGCGCCCGGAAGAAGTGACGGAGATGCAGCGGTCGGTGAAGGGCGAGGTTGTGGCCT
CCACCTTCGACGAACCCGCGTCGCGCCATGTGCAAGTCGCCGAAATGGTGATCGAGAAGGCCAAGCGCCTGACCGAGCAC
AAGAAGGACGTGGTCATCCTGCTGGACTCCCTTACCCGGCTTGCCCGCGCCTACAACACCGTGGTGCCCGCTTCCGGCAA
GGTGTTGACCGGCGGTGTCGACGCCAATGCGCTGCAGAAGCCCAAGCGTTTCTTCGGTGCGGCGCGCAACATCGAGGAAG
GTGGCTCGCTGACCATCATCGCCACCGCGCTGATCGACACCGGCAGCCGCATGGACGACGTGATCTACGAGGAGTTCAAG
GGCACCGGCAACATGGAGCTCCACCTCGACCGACGCATGGCGGAGAAGCGCGTCTATCCGGCAATCAACGTCAATCGCTC
CGGCACCCGGCGCGAAGAACTCCTGATGAAGCCGGACGTGCTGCAGAAGATCTGGATACTGCGCAAGCTGCTGTATGGCA
TGGACGACATCGACGCGATGGAATTCCTGCTCGACAAGGTCAAGGCCACCAAGGGCAACGCCGAATTCTTCGATGCAATG
AGGCGCGGATAG

Upstream 100 bases:

>100_bases
TACGGCATCCGTGGCATTCCGACCCTGATGATCTTCAAGGGCGGCAACATCGAGGCGACCAAGGTTGGCGCCCTGTCCAA
ATCTCAACTGACTGCTTTCA

Downstream 100 bases:

>100_bases
TCCCGACAGCACAAAACAAAAGCCACCCCGGGGTGGCTTTTGTATTTTCTGTAGCGCCTAATTTAGGCGAGGCGCCTCAG
GCGTAGTCGAAGAAGCCCTT

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MTATSKPEATPARSRGASRTRRRGARNRDGNSPQGNTSELDLLDAELAMEEGSASSAESTAAAALHLSELKALHVSQLLE
MAVANEIEGANRLRKQELVFALLKNRARKGEPIYGDGALEVLPDGFGFLRSPDTSYLAGTDDIYVSPSQIRRFNLHTGDT
IEGEIRTPKDGERYFALVKLDKINGRPPEECKHKILFENLTPLHPQECLKLEREIRGEENVTSRIIDMIAPIGKGQRGLL
VAPPKSGKTVMLQHIAHAITANHPDVELIVLLIDERPEEVTEMQRSVKGEVVASTFDEPASRHVQVAEMVIEKAKRLTEH
KKDVVILLDSLTRLARAYNTVVPASGKVLTGGVDANALQKPKRFFGAARNIEEGGSLTIIATALIDTGSRMDDVIYEEFK
GTGNMELHLDRRMAEKRVYPAINVNRSGTRREELLMKPDVLQKIWILRKLLYGMDDIDAMEFLLDKVKATKGNAEFFDAM
RRG

Sequences:

>Translated_483_residues
MTATSKPEATPARSRGASRTRRRGARNRDGNSPQGNTSELDLLDAELAMEEGSASSAESTAAAALHLSELKALHVSQLLE
MAVANEIEGANRLRKQELVFALLKNRARKGEPIYGDGALEVLPDGFGFLRSPDTSYLAGTDDIYVSPSQIRRFNLHTGDT
IEGEIRTPKDGERYFALVKLDKINGRPPEECKHKILFENLTPLHPQECLKLEREIRGEENVTSRIIDMIAPIGKGQRGLL
VAPPKSGKTVMLQHIAHAITANHPDVELIVLLIDERPEEVTEMQRSVKGEVVASTFDEPASRHVQVAEMVIEKAKRLTEH
KKDVVILLDSLTRLARAYNTVVPASGKVLTGGVDANALQKPKRFFGAARNIEEGGSLTIIATALIDTGSRMDDVIYEEFK
GTGNMELHLDRRMAEKRVYPAINVNRSGTRREELLMKPDVLQKIWILRKLLYGMDDIDAMEFLLDKVKATKGNAEFFDAM
RRG
>Mature_482_residues
TATSKPEATPARSRGASRTRRRGARNRDGNSPQGNTSELDLLDAELAMEEGSASSAESTAAAALHLSELKALHVSQLLEM
AVANEIEGANRLRKQELVFALLKNRARKGEPIYGDGALEVLPDGFGFLRSPDTSYLAGTDDIYVSPSQIRRFNLHTGDTI
EGEIRTPKDGERYFALVKLDKINGRPPEECKHKILFENLTPLHPQECLKLEREIRGEENVTSRIIDMIAPIGKGQRGLLV
APPKSGKTVMLQHIAHAITANHPDVELIVLLIDERPEEVTEMQRSVKGEVVASTFDEPASRHVQVAEMVIEKAKRLTEHK
KDVVILLDSLTRLARAYNTVVPASGKVLTGGVDANALQKPKRFFGAARNIEEGGSLTIIATALIDTGSRMDDVIYEEFKG
TGNMELHLDRRMAEKRVYPAINVNRSGTRREELLMKPDVLQKIWILRKLLYGMDDIDAMEFLLDKVKATKGNAEFFDAMR
RG

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=418, Percent_Identity=70.3349282296651, Blast_Score=617, Evalue=1e-178,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 53559; Mature: 53428

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATSKPEATPARSRGASRTRRRGARNRDGNSPQGNTSELDLLDAELAMEEGSASSAEST
CCCCCCCCCCCHHHCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHH
AAAALHLSELKALHVSQLLEMAVANEIEGANRLRKQELVFALLKNRARKGEPIYGDGALE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
VLPDGFGFLRSPDTSYLAGTDDIYVSPSQIRRFNLHTGDTIEGEIRTPKDGERYFALVKL
ECCCCCCCCCCCCCCEECCCCCEEECHHHHHEEECCCCCCCCCCCCCCCCCCEEEEEEEE
DKINGRPPEECKHKILFENLTPLHPQECLKLEREIRGEENVTSRIIDMIAPIGKGQRGLL
ECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEE
VAPPKSGKTVMLQHIAHAITANHPDVELIVLLIDERPEEVTEMQRSVKGEVVASTFDEPA
EECCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCCHHHHHHCCHH
SRHVQVAEMVIEKAKRLTEHKKDVVILLDSLTRLARAYNTVVPASGKVLTGGVDANALQK
HHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHH
PKRFFGAARNIEEGGSLTIIATALIDTGSRMDDVIYEEFKGTGNMELHLDRRMAEKRVYP
HHHHHHHHCCCCCCCCEEEEEEEHHCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCC
AINVNRSGTRREELLMKPDVLQKIWILRKLLYGMDDIDAMEFLLDKVKATKGNAEFFDAM
CEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHH
RRG
HCC
>Mature Secondary Structure 
TATSKPEATPARSRGASRTRRRGARNRDGNSPQGNTSELDLLDAELAMEEGSASSAEST
CCCCCCCCCCHHHCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHH
AAAALHLSELKALHVSQLLEMAVANEIEGANRLRKQELVFALLKNRARKGEPIYGDGALE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
VLPDGFGFLRSPDTSYLAGTDDIYVSPSQIRRFNLHTGDTIEGEIRTPKDGERYFALVKL
ECCCCCCCCCCCCCCEECCCCCEEECHHHHHEEECCCCCCCCCCCCCCCCCCEEEEEEEE
DKINGRPPEECKHKILFENLTPLHPQECLKLEREIRGEENVTSRIIDMIAPIGKGQRGLL
ECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEE
VAPPKSGKTVMLQHIAHAITANHPDVELIVLLIDERPEEVTEMQRSVKGEVVASTFDEPA
EECCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCCHHHHHHCCHH
SRHVQVAEMVIEKAKRLTEHKKDVVILLDSLTRLARAYNTVVPASGKVLTGGVDANALQK
HHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHH
PKRFFGAARNIEEGGSLTIIATALIDTGSRMDDVIYEEFKGTGNMELHLDRRMAEKRVYP
HHHHHHHHCCCCCCCCEEEEEEEHHCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCC
AINVNRSGTRREELLMKPDVLQKIWILRKLLYGMDDIDAMEFLLDKVKATKGNAEFFDAM
CEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHH
RRG
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]