Click here to switch to the map view.

The map label for this gene is glcF [H]

Identifier: 119897292

GI number: 119897292

Start: 1075575

End: 1076819

Strand: Direct

Name: glcF [H]

Synonym: azo1001

Alternate gene names: 119897292

Gene position: 1075575-1076819 (Clockwise)

Preceding gene: 119897291

Following gene: 119897293

Centisome position: 24.58

GC content: 69.48

Gene sequence:

>1245_bases
ATGCAGACCCAGCTTGCCGATTTCATCCGCGATACGCCTGAAGGCCGCGAGGCTGACGAGATCCTGCGCAAATGCGTGCA
TTGCGGCTTCTGCACCGCCACCTGTCCGACCTACCAGTTGCTCGGCGACGAACTGGATGGTCCGCGCGGGCGGATCTACC
TGATCAAGCAGTTGCTCGAAGGCCAGCCGGTGACCGAGAAGACCCGCCTGCACCTCGATCGCTGCCTCACCTGCCGGGCC
TGTGAAACCACCTGTCCGTCCGGGGTGCACTACAGCCGGCTGGCGGACATCGGCCGTCATCTGGTCGAGCAGCGGGTCCC
GCGCCGCGGGGCGGATCGCGCCGCGCGCTGGGTGCTGCGCGAACTGGTGCCGCGTGCGGCCGTCTTCGGCGCCGCGCTGA
AGGCGGGCCGCAGCGTGCGTGGGATGCTGCCGGCGACGCTGCGCGACAAGGTGCCCATGGTGCGTGCGCCGGGGCCGTGG
CCGCGGCCGCGTCATGCGCGCCGCATGTACGCGCTGGCAGGGTGCGCGCAACCCGCAATGGCGCCGTCGATCAACGCCGC
GACCGCACGGGTGCTGGACAGCCTCGGCATCTCGCTGCTGGAGGCGCCCTCCGCCGGCTGCTGTGGCGCGGTCCGTTTCC
ATCTCCACGATCACGATGGCGCGCGCGCCGAAGCCCGGCGCAATATCGACGCCTGGTGGCCGGCGATCGAGGGCGGCGAG
GTGGAAGGCCTGGTGATGACCGCATCGGGCTGCGGTGTGCAGGTGAAAGACTACGGGCACCTGCTGCAGCACGACCCCGC
CTATTCCGCCAAAGCCGCGCGCATCGCCGCGCTGACGCGCGACGTCGCCGAGGTGGTAAGCGCCCAGGAGGCGTCGCTGA
AGGCGCTGCTGCGCAGCAAGACGGGCGGGGAAACGGCGGTTGCCTGGCACGCCCCATGCACGCTGCAGCACGGCCTGAAG
ATCCGCGGTGCGGTGGAGCAATTGCTGGGTGCTGCCGGCTTTCGCCTGACCCCGGTGCGCGACGCCCACCTGTGTTGCGG
TTCCGCGGGAACGTACTCGCTGCTGCAGCCCGAAATCGCCCAGCAACTGCGGGAAAACAAGCTCGCGGCGCTCGGCGAAG
GCGGTGCGCCACTCATACTTTCGGCCAATATCGGCTGCATTACCCACTTGCAGGCGGGCACGGCGACCCCCGTCCGGCAC
TGGATCGAGGCGATAGACGCGCGGCTCAACGGCTTCCCAATTTGA

Upstream 100 bases:

>100_bases
CTTGCCGGAGCCGCTGCTGCGCATTCATCGCCGCCTGAAGGCCGCATTCGATCCCGCGGGCATCTTCAACCGCGGGCGCC
TCTACCAAGGACTTTGACGC

Downstream 100 bases:

>100_bases
GCAGTCATGGTGCTGTGATGCAGGTCACAAACGGCATCCGGCGGCGGCTTTTCCGTGACCGGCGGCGGGGCGGGAGGGTG
GGGTCATTGTTATGATGCGA

Product: glycolate oxidase iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MQTQLADFIRDTPEGREADEILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQLLEGQPVTEKTRLHLDRCLTCRA
CETTCPSGVHYSRLADIGRHLVEQRVPRRGADRAARWVLRELVPRAAVFGAALKAGRSVRGMLPATLRDKVPMVRAPGPW
PRPRHARRMYALAGCAQPAMAPSINAATARVLDSLGISLLEAPSAGCCGAVRFHLHDHDGARAEARRNIDAWWPAIEGGE
VEGLVMTASGCGVQVKDYGHLLQHDPAYSAKAARIAALTRDVAEVVSAQEASLKALLRSKTGGETAVAWHAPCTLQHGLK
IRGAVEQLLGAAGFRLTPVRDAHLCCGSAGTYSLLQPEIAQQLRENKLAALGEGGAPLILSANIGCITHLQAGTATPVRH
WIEAIDARLNGFPI

Sequences:

>Translated_414_residues
MQTQLADFIRDTPEGREADEILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQLLEGQPVTEKTRLHLDRCLTCRA
CETTCPSGVHYSRLADIGRHLVEQRVPRRGADRAARWVLRELVPRAAVFGAALKAGRSVRGMLPATLRDKVPMVRAPGPW
PRPRHARRMYALAGCAQPAMAPSINAATARVLDSLGISLLEAPSAGCCGAVRFHLHDHDGARAEARRNIDAWWPAIEGGE
VEGLVMTASGCGVQVKDYGHLLQHDPAYSAKAARIAALTRDVAEVVSAQEASLKALLRSKTGGETAVAWHAPCTLQHGLK
IRGAVEQLLGAAGFRLTPVRDAHLCCGSAGTYSLLQPEIAQQLRENKLAALGEGGAPLILSANIGCITHLQAGTATPVRH
WIEAIDARLNGFPI
>Mature_414_residues
MQTQLADFIRDTPEGREADEILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQLLEGQPVTEKTRLHLDRCLTCRA
CETTCPSGVHYSRLADIGRHLVEQRVPRRGADRAARWVLRELVPRAAVFGAALKAGRSVRGMLPATLRDKVPMVRAPGPW
PRPRHARRMYALAGCAQPAMAPSINAATARVLDSLGISLLEAPSAGCCGAVRFHLHDHDGARAEARRNIDAWWPAIEGGE
VEGLVMTASGCGVQVKDYGHLLQHDPAYSAKAARIAALTRDVAEVVSAQEASLKALLRSKTGGETAVAWHAPCTLQHGLK
IRGAVEQLLGAAGFRLTPVRDAHLCCGSAGTYSLLQPEIAQQLRENKLAALGEGGAPLILSANIGCITHLQAGTATPVRH
WIEAIDARLNGFPI

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=411, Percent_Identity=56.2043795620438, Blast_Score=448, Evalue=1e-127,
Organism=Escherichia coli, GI1786497, Length=242, Percent_Identity=22.7272727272727, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 44605; Mature: 44605

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTQLADFIRDTPEGREADEILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQLLE
CCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHC
GQPVTEKTRLHLDRCLTCRACETTCPSGVHYSRLADIGRHLVEQRVPRRGADRAARWVLR
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ELVPRAAVFGAALKAGRSVRGMLPATLRDKVPMVRAPGPWPRPRHARRMYALAGCAQPAM
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCEEECCCCCCCCHHHHHHHHHHHCCCCCC
APSINAATARVLDSLGISLLEAPSAGCCGAVRFHLHDHDGARAEARRNIDAWWPAIEGGE
CCCCHHHHHHHHHHHCHHHHCCCCCCCCEEEEEEEECCCCCHHHHHCCCHHHCCCCCCCC
VEGLVMTASGCGVQVKDYGHLLQHDPAYSAKAARIAALTRDVAEVVSAQEASLKALLRSK
CCEEEEEECCCCEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TGGETAVAWHAPCTLQHGLKIRGAVEQLLGAAGFRLTPVRDAHLCCGSAGTYSLLQPEIA
CCCCEEEEEECCCHHHCCCHHHHHHHHHHHCCCCEECCCCCHHHHCCCCCCHHHCCHHHH
QQLRENKLAALGEGGAPLILSANIGCITHLQAGTATPVRHWIEAIDARLNGFPI
HHHHHHHHHHCCCCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQTQLADFIRDTPEGREADEILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQLLE
CCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHC
GQPVTEKTRLHLDRCLTCRACETTCPSGVHYSRLADIGRHLVEQRVPRRGADRAARWVLR
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ELVPRAAVFGAALKAGRSVRGMLPATLRDKVPMVRAPGPWPRPRHARRMYALAGCAQPAM
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCEEECCCCCCCCHHHHHHHHHHHCCCCCC
APSINAATARVLDSLGISLLEAPSAGCCGAVRFHLHDHDGARAEARRNIDAWWPAIEGGE
CCCCHHHHHHHHHHHCHHHHCCCCCCCCEEEEEEEECCCCCHHHHHCCCHHHCCCCCCCC
VEGLVMTASGCGVQVKDYGHLLQHDPAYSAKAARIAALTRDVAEVVSAQEASLKALLRSK
CCEEEEEECCCCEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TGGETAVAWHAPCTLQHGLKIRGAVEQLLGAAGFRLTPVRDAHLCCGSAGTYSLLQPEIA
CCCCEEEEEECCCHHHCCCHHHHHHHHHHHCCCCEECCCCCHHHHCCCCCCHHHCCHHHH
QQLRENKLAALGEGGAPLILSANIGCITHLQAGTATPVRHWIEAIDARLNGFPI
HHHHHHHHHHCCCCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]