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The map label for this gene is glcE [H]

Identifier: 119897291

GI number: 119897291

Start: 1074510

End: 1075571

Strand: Direct

Name: glcE [H]

Synonym: azo1000

Alternate gene names: 119897291

Gene position: 1074510-1075571 (Clockwise)

Preceding gene: 119897290

Following gene: 119897292

Centisome position: 24.55

GC content: 72.22

Gene sequence:

>1062_bases
ATGACGGACATCGTTTCAGCCTGGGCCGATCGCATCCGCGCGGCGGCCGCGGCGGATACGCCGCTGCAGTTGCGCGGTGG
CGGCACGAAGGCCTTCTATGGCCGGTCTGGCGAGGGCGAGGTGCTCGACCTGACGGATAACCGCGGCGTCGTCAGCTACG
AACCCACCGAGCTGGTGGTCACGGTCCGCGGCGGCACGCCGCTCGCCGAACTCGAAGCCCTGCTCGCCCGTCACGACCAG
TTCCTCGCCTTCGAGCCGCCGGCGTTCGGGCCGGCGGCCACCGTGGGTGGCTGTGTGGCCGCCGGCCTGTCCGGCCCGCG
GCGTGCGAGCGCCGGTGCGGTGCGCGACTTCGTGCTCGGCGCCCGGCTGCTCGACGGGCGCGGCGAGGCGCTGTCGTTTG
GCGGCCAGGTGATGAAGAACGTGGCCGGCTACGACGTGTCTCGTTTGCTGGCGGGCAGCCTCGGTACGCTGGGCGTCCTG
CTGGAAGTGTCGCTGAAGGTGTTGCCGCGTCCCGCCGCCGAGGCCACGCTGCGTTTTGAATGCGACGAGGCGAGCGCGAT
CCAGCGCCTCAACGACTGGGGCGGACAACCGCTGCCGCTGTCGGCCTCCACCTGGCATGACGGCGTGCTGACGGTGCGCC
TGTCGGGCGCGAGCGCAGCGGTCGCCGCCGCCCAGGCGCGGCTGGGCGGCGAACGGCTCGACGAGGCGCTCGCGACGGCA
TTCTGGACCAGCATCCGCGAGCACACCGCGCCATGGTTTGCCGCGAGGCCGCTATGGCGCCTGTCGCTGCCGAGTTCGGC
GGCGCCGCTGCGGCTGGCGGGCGGGCAGTTGATCGAATGGGGCGGCGCGCTGCGCTGGCTGCATACCGACCTGCCCGCGA
CAGCCGTGCGCGAGCGCGTCGCGCAACTCGGCGGCACTGCCGCGCTCTTCGCCGGCGGCAACCGCAGCGGGGAGGTCTTT
CATCCCTTGCCGGAGCCGCTGCTGCGCATTCATCGCCGCCTGAAGGCCGCATTCGATCCCGCGGGCATCTTCAACCGCGG
GCGCCTCTACCAAGGACTTTGA

Upstream 100 bases:

>100_bases
CGGTGCTGTCGCTCGTCGGGGCGTGGCTGGCGCTGCGCGCGGTCGGTGGGCGCAATGGGCGCCGGCCGCAAGGCAAACAA
CAACGCGAGGCGAAAGAGCA

Downstream 100 bases:

>100_bases
CGCATGCAGACCCAGCTTGCCGATTTCATCCGCGATACGCCTGAAGGCCGCGAGGCTGACGAGATCCTGCGCAAATGCGT
GCATTGCGGCTTCTGCACCG

Product: glycolate oxidase FAD binding subunit

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MTDIVSAWADRIRAAAAADTPLQLRGGGTKAFYGRSGEGEVLDLTDNRGVVSYEPTELVVTVRGGTPLAELEALLARHDQ
FLAFEPPAFGPAATVGGCVAAGLSGPRRASAGAVRDFVLGARLLDGRGEALSFGGQVMKNVAGYDVSRLLAGSLGTLGVL
LEVSLKVLPRPAAEATLRFECDEASAIQRLNDWGGQPLPLSASTWHDGVLTVRLSGASAAVAAAQARLGGERLDEALATA
FWTSIREHTAPWFAARPLWRLSLPSSAAPLRLAGGQLIEWGGALRWLHTDLPATAVRERVAQLGGTAALFAGGNRSGEVF
HPLPEPLLRIHRRLKAAFDPAGIFNRGRLYQGL

Sequences:

>Translated_353_residues
MTDIVSAWADRIRAAAAADTPLQLRGGGTKAFYGRSGEGEVLDLTDNRGVVSYEPTELVVTVRGGTPLAELEALLARHDQ
FLAFEPPAFGPAATVGGCVAAGLSGPRRASAGAVRDFVLGARLLDGRGEALSFGGQVMKNVAGYDVSRLLAGSLGTLGVL
LEVSLKVLPRPAAEATLRFECDEASAIQRLNDWGGQPLPLSASTWHDGVLTVRLSGASAAVAAAQARLGGERLDEALATA
FWTSIREHTAPWFAARPLWRLSLPSSAAPLRLAGGQLIEWGGALRWLHTDLPATAVRERVAQLGGTAALFAGGNRSGEVF
HPLPEPLLRIHRRLKAAFDPAGIFNRGRLYQGL
>Mature_352_residues
TDIVSAWADRIRAAAAADTPLQLRGGGTKAFYGRSGEGEVLDLTDNRGVVSYEPTELVVTVRGGTPLAELEALLARHDQF
LAFEPPAFGPAATVGGCVAAGLSGPRRASAGAVRDFVLGARLLDGRGEALSFGGQVMKNVAGYDVSRLLAGSLGTLGVLL
EVSLKVLPRPAAEATLRFECDEASAIQRLNDWGGQPLPLSASTWHDGVLTVRLSGASAAVAAAQARLGGERLDEALATAF
WTSIREHTAPWFAARPLWRLSLPSSAAPLRLAGGQLIEWGGALRWLHTDLPATAVRERVAQLGGTAALFAGGNRSGEVFH
PLPEPLLRIHRRLKAAFDPAGIFNRGRLYQGL

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Escherichia coli, GI48994914, Length=350, Percent_Identity=47.4285714285714, Blast_Score=311, Evalue=3e-86,
Organism=Saccharomyces cerevisiae, GI6320027, Length=142, Percent_Identity=29.5774647887324, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 37190; Mature: 37059

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIVSAWADRIRAAAAADTPLQLRGGGTKAFYGRSGEGEVLDLTDNRGVVSYEPTELVV
CCHHHHHHHHHHHHHHCCCCCEEEECCCCEEECCCCCCCCEEEECCCCCEEEECCCEEEE
TVRGGTPLAELEALLARHDQFLAFEPPAFGPAATVGGCVAAGLSGPRRASAGAVRDFVLG
EEECCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
ARLLDGRGEALSFGGQVMKNVAGYDVSRLLAGSLGTLGVLLEVSLKVLPRPAAEATLRFE
HHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEE
CDEASAIQRLNDWGGQPLPLSASTWHDGVLTVRLSGASAAVAAAQARLGGERLDEALATA
CCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCHHHHHHHHHHH
FWTSIREHTAPWFAARPLWRLSLPSSAAPLRLAGGQLIEWGGALRWLHTDLPATAVRERV
HHHHHHHHCCCCHHHCCEEEEECCCCCCCEEECCCCEEECCCEEEEEECCCCHHHHHHHH
AQLGGTAALFAGGNRSGEVFHPLPEPLLRIHRRLKAAFDPAGIFNRGRLYQGL
HHHCCCEEEEECCCCCCCEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDIVSAWADRIRAAAAADTPLQLRGGGTKAFYGRSGEGEVLDLTDNRGVVSYEPTELVV
CHHHHHHHHHHHHHHCCCCCEEEECCCCEEECCCCCCCCEEEECCCCCEEEECCCEEEE
TVRGGTPLAELEALLARHDQFLAFEPPAFGPAATVGGCVAAGLSGPRRASAGAVRDFVLG
EEECCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
ARLLDGRGEALSFGGQVMKNVAGYDVSRLLAGSLGTLGVLLEVSLKVLPRPAAEATLRFE
HHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEE
CDEASAIQRLNDWGGQPLPLSASTWHDGVLTVRLSGASAAVAAAQARLGGERLDEALATA
CCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCHHHHHHHHHHH
FWTSIREHTAPWFAARPLWRLSLPSSAAPLRLAGGQLIEWGGALRWLHTDLPATAVRERV
HHHHHHHHCCCCHHHCCEEEEECCCCCCCEEECCCCEEECCCEEEEEECCCCHHHHHHHH
AQLGGTAALFAGGNRSGEVFHPLPEPLLRIHRRLKAAFDPAGIFNRGRLYQGL
HHHCCCEEEEECCCCCCCEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]