Click here to switch to the map view.

The map label for this gene is purA [H]

Identifier: 119897229

GI number: 119897229

Start: 1003629

End: 1004939

Strand: Direct

Name: purA [H]

Synonym: azo0938

Alternate gene names: 119897229

Gene position: 1003629-1004939 (Clockwise)

Preceding gene: 119897228

Following gene: 119897230

Centisome position: 22.93

GC content: 65.22

Gene sequence:

>1311_bases
ATGGCAAAGAACGTAGTGGTCGTCGGCACCCAGTGGGGCGACGAGGGCAAGGGCAAGATCGTCGACTGGCTGACGGATCA
TGCGCGCGGTGTCGTGCGTTTCCAGGGCGGCCATAATGCCGGCCACACGCTGGTGATCGGGCAGAAGGAGTACAAGCTCA
ACCTGGTGCCGTCGGGCATCGTGCGGGAAGGCGTGGCGTGTTACATCGGCAATGGCGTGGTGCTGGACGCCCACCATCTG
CTGTCCGAAATCCGCGTGCTGGAGGCGGGCGGAATCAAGGTACGCGAGCGTCTGCGGATCAGCCCGGGCTGTCCGCTGAT
CCTCGGCTACCATGCCGCGCTCGACCGGGCGCGCGAGGCTGCGAAGGCCGCCTGCGACAAGATCGGCACCACGGGCAAGG
GCATCGGCCCCACGTATGAGGACAAGGTCGCCCGCCGCGCGCTGCGGGTGTACGACCTTTTCGATCGCGAACGCTTCGCA
GCCAAGCTGAAGGCGAACCTCGAATACCACAACTTCGTGCTGACCCAGCACCTTGGGGCCGAACCGGTCGGCTTCGACGA
GGTGTTCGACGAGGCAATGGCCGACGCGGCAGAAATCCTGCCGATGGTGGCGGACGTTTCCGCGGAGCTGTACGCGGTGA
ACAAGGCGGGCGGCAGCCTGCTGTTCGAAGGCGCCCAGGGCACGCTGCTCGATATCGACCACGGCACCTATCCGTTCGTC
ACTTCCAGCAACTGCGTGGCGGGGCAGGCGGCAGCCGGGTCCGGCGTTGGGCCTGGCCGTCTCCATTATGTGCTTGGGAT
CACCAAGGCGTACTGCACGCGCGTCGGTGGCGGCCCGTTCCCGACTGAACTGGACATTGAAACCCCGGGTACGCCGGGCC
AGCAGATGTCGACCAAGGGGCGCGAATTTGGCACCGTCACCGGGCGCAAGCGCCGCTGCGGCTGGCTCGACCTCGCCGCG
CTCAAGCGTTCCATCATCATCAATGGTGTGACCGGGCTGTGCATCACCAAGCTTGACGTCCTCGACGGGTTGGCCGAGCT
GAAGCTGTGCACCGGATACATGCTGGACGGCAAGCGGATCGACCTGTTGCCGATGGGGTCGGAAGAAGTGACGCGCTGCG
AACCGATCTACGAAACGCTGTCGGGCTGGTCGGGCACCACCTTCGGTGCGCAAAGTTGGGACGCGCTGCCGCAGGAGGCG
CGCGCCTATCTGCACCGGATCGAGGAAATCTGCGAAATTCCGATCGACGTCATCTCGACCGGACCGGAGCGGGACGAAAC
CATCTTGCGGCGCCATCCCTTCGGTGCCTGA

Upstream 100 bases:

>100_bases
GCCGGGACACGAAGGAACATGGAATGACGCTGGCTGCGATCGGCAGCTGGTGATGCGGCAAGGCGCGTGGGTCGTCGAGC
CGCTTCAGGGAGAGTAAGGA

Downstream 100 bases:

>100_bases
GTTCGATGCAGTCGAAGCCGGTCGCGAGACCGGCTTGTTGTTTTGCGGGGCGGTTTGCCTCGGGCGGTGCTGTTACGTGC
GGGTGGAGTCGCGCGTTGCC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MAKNVVVVGTQWGDEGKGKIVDWLTDHARGVVRFQGGHNAGHTLVIGQKEYKLNLVPSGIVREGVACYIGNGVVLDAHHL
LSEIRVLEAGGIKVRERLRISPGCPLILGYHAALDRAREAAKAACDKIGTTGKGIGPTYEDKVARRALRVYDLFDRERFA
AKLKANLEYHNFVLTQHLGAEPVGFDEVFDEAMADAAEILPMVADVSAELYAVNKAGGSLLFEGAQGTLLDIDHGTYPFV
TSSNCVAGQAAAGSGVGPGRLHYVLGITKAYCTRVGGGPFPTELDIETPGTPGQQMSTKGREFGTVTGRKRRCGWLDLAA
LKRSIIINGVTGLCITKLDVLDGLAELKLCTGYMLDGKRIDLLPMGSEEVTRCEPIYETLSGWSGTTFGAQSWDALPQEA
RAYLHRIEEICEIPIDVISTGPERDETILRRHPFGA

Sequences:

>Translated_436_residues
MAKNVVVVGTQWGDEGKGKIVDWLTDHARGVVRFQGGHNAGHTLVIGQKEYKLNLVPSGIVREGVACYIGNGVVLDAHHL
LSEIRVLEAGGIKVRERLRISPGCPLILGYHAALDRAREAAKAACDKIGTTGKGIGPTYEDKVARRALRVYDLFDRERFA
AKLKANLEYHNFVLTQHLGAEPVGFDEVFDEAMADAAEILPMVADVSAELYAVNKAGGSLLFEGAQGTLLDIDHGTYPFV
TSSNCVAGQAAAGSGVGPGRLHYVLGITKAYCTRVGGGPFPTELDIETPGTPGQQMSTKGREFGTVTGRKRRCGWLDLAA
LKRSIIINGVTGLCITKLDVLDGLAELKLCTGYMLDGKRIDLLPMGSEEVTRCEPIYETLSGWSGTTFGAQSWDALPQEA
RAYLHRIEEICEIPIDVISTGPERDETILRRHPFGA
>Mature_435_residues
AKNVVVVGTQWGDEGKGKIVDWLTDHARGVVRFQGGHNAGHTLVIGQKEYKLNLVPSGIVREGVACYIGNGVVLDAHHLL
SEIRVLEAGGIKVRERLRISPGCPLILGYHAALDRAREAAKAACDKIGTTGKGIGPTYEDKVARRALRVYDLFDRERFAA
KLKANLEYHNFVLTQHLGAEPVGFDEVFDEAMADAAEILPMVADVSAELYAVNKAGGSLLFEGAQGTLLDIDHGTYPFVT
SSNCVAGQAAAGSGVGPGRLHYVLGITKAYCTRVGGGPFPTELDIETPGTPGQQMSTKGREFGTVTGRKRRCGWLDLAAL
KRSIIINGVTGLCITKLDVLDGLAELKLCTGYMLDGKRIDLLPMGSEEVTRCEPIYETLSGWSGTTFGAQSWDALPQEAR
AYLHRIEEICEIPIDVISTGPERDETILRRHPFGA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=435, Percent_Identity=42.9885057471264, Blast_Score=318, Evalue=7e-87,
Organism=Homo sapiens, GI34577063, Length=434, Percent_Identity=43.778801843318, Blast_Score=307, Evalue=2e-83,
Organism=Homo sapiens, GI40316944, Length=402, Percent_Identity=42.0398009950249, Blast_Score=283, Evalue=2e-76,
Organism=Escherichia coli, GI1790620, Length=437, Percent_Identity=58.3524027459954, Blast_Score=503, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI25146553, Length=436, Percent_Identity=41.2844036697248, Blast_Score=316, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI25146555, Length=436, Percent_Identity=41.2844036697248, Blast_Score=316, Evalue=2e-86,
Organism=Saccharomyces cerevisiae, GI6324109, Length=433, Percent_Identity=40.8775981524249, Blast_Score=313, Evalue=5e-86,
Organism=Drosophila melanogaster, GI21356033, Length=432, Percent_Identity=39.5833333333333, Blast_Score=290, Evalue=2e-78,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47040; Mature: 46909

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNVVVVGTQWGDEGKGKIVDWLTDHARGVVRFQGGHNAGHTLVIGQKEYKLNLVPSGI
CCCCEEEEEECCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCEEEEECCCEEEEECCCHH
VREGVACYIGNGVVLDAHHLLSEIRVLEAGGIKVRERLRISPGCPLILGYHAALDRAREA
HHCCCEEEECCCEEEEHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEHHHHHHHHHHHH
AKAACDKIGTTGKGIGPTYEDKVARRALRVYDLFDRERFAAKLKANLEYHNFVLTQHLGA
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEECCCCC
EPVGFDEVFDEAMADAAEILPMVADVSAELYAVNKAGGSLLFEGAQGTLLDIDHGTYPFV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCCCEEECCCCCCCEE
TSSNCVAGQAAAGSGVGPGRLHYVLGITKAYCTRVGGGPFPTELDIETPGTPGQQMSTKG
ECCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHCCC
REFGTVTGRKRRCGWLDLAALKRSIIINGVTGLCITKLDVLDGLAELKLCTGYMLDGKRI
CCCCCCCCCCCCCCHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHCEEECCCEE
DLLPMGSEEVTRCEPIYETLSGWSGTTFGAQSWDALPQEARAYLHRIEEICEIPIDVIST
EEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCC
GPERDETILRRHPFGA
CCCCHHHHHHCCCCCC
>Mature Secondary Structure 
AKNVVVVGTQWGDEGKGKIVDWLTDHARGVVRFQGGHNAGHTLVIGQKEYKLNLVPSGI
CCCEEEEEECCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCEEEEECCCEEEEECCCHH
VREGVACYIGNGVVLDAHHLLSEIRVLEAGGIKVRERLRISPGCPLILGYHAALDRAREA
HHCCCEEEECCCEEEEHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEHHHHHHHHHHHH
AKAACDKIGTTGKGIGPTYEDKVARRALRVYDLFDRERFAAKLKANLEYHNFVLTQHLGA
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEECCCCC
EPVGFDEVFDEAMADAAEILPMVADVSAELYAVNKAGGSLLFEGAQGTLLDIDHGTYPFV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCCCEEECCCCCCCEE
TSSNCVAGQAAAGSGVGPGRLHYVLGITKAYCTRVGGGPFPTELDIETPGTPGQQMSTKG
ECCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHCCC
REFGTVTGRKRRCGWLDLAALKRSIIINGVTGLCITKLDVLDGLAELKLCTGYMLDGKRI
CCCCCCCCCCCCCCHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHCEEECCCEE
DLLPMGSEEVTRCEPIYETLSGWSGTTFGAQSWDALPQEARAYLHRIEEICEIPIDVIST
EEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCC
GPERDETILRRHPFGA
CCCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA