Click here to switch to the map view.

The map label for this gene is hisZ

Identifier: 119897228

GI number: 119897228

Start: 1002471

End: 1003625

Strand: Direct

Name: hisZ

Synonym: azo0937

Alternate gene names: 119897228

Gene position: 1002471-1003625 (Clockwise)

Preceding gene: 119897227

Following gene: 119897229

Centisome position: 22.91

GC content: 68.66

Gene sequence:

>1155_bases
ATGCGCTGGGTATTGCCCGATCACATTCAGGACGCGCTGCCGTCCGAGGCAGCCAGCCTCGAGGCGTTGCGCCGCCGCCT
GCTGGACGCATTCCGCGTCCGGGGCTATCAACTGGTGATGCCGCCGCTGCTCGAGTACCTGGACTCTCTGACCACGGGCG
CCGGTCAGGACCTGAAGCTGCGCACCTTCAAGCTGGTCGATCAGGTATCGGGCCGGACCATGGGCGTACGGGCCGACATG
ACGCCGCAGGTCACGCGCATCGATGCGCACCTGCTCAACCGCGCTGGCGTGTCCCGGCTGTGCTACTGCGGCAGCGTGTT
GCATACGCTTCCATCGACGCTGACGGCGACGCGCGAGCCCCTGCAACTGGGGGCGGAGCTTTACGGCCACGCAGGCATCG
ATGCCGACATCGAAATCGTCCGCCTGCTGGCGGACGTGCTGCGCCTGGCCGAAGTGCCGGCGAGTCGCATCGATATCGGT
CATGTCGGTCTGTTCCATGCCTTGGCGGCACTGGCGGGGATGGTGCCCGAGCGCGAAGAAGAGCTGTTCGATCTGCTGCA
GGCCAAGGACGTGCCGGGCCTTAAGGAGATCACCGTCGGTGTTGCCGAGCCGGTCCGGACCGCACTGCTGCGCCTGCCTG
CGTTGTACGGCGGGGCGGAGGTCATAGACGAGGCGGCAGCGTGCATGCCCGAGTCGGCGGAGATCCGCGCCGCGCTCGAC
GACCTGCGGCGGCTCGCCGCGGCGCTGGAAGACCTGCCGATCAGTTTCGATCTGGCCGACTTGCGGGGCTACCACTATCA
CAGCGGGGTGGTGTTCGCCGCGTATGGCGGTGGCTCGCCCGCGGCGCTGGCGCTCGGCGGGCGGTACGACCGCGTCGGTG
AAGCCTTTGGTCGTGCCCGCCCGGCGACCGGCTTCAGTCTTGATCTGCGGGAACTCGCGCTGCGTCTGCCGGCGGCAGTC
GTGCCCGGGGCCATTCTCGCGCCGCTGGAAGGTACCGCGGGTCTCGCGGCCGCGGTCGAGGCATTGCGCGCCGCCGGTGA
GGCGGTGATGTCCCGCCTGCCGGGACACGAAGGAACATGGAATGACGCTGGCTGCGATCGGCAGCTGGTGATGCGGCAAG
GCGCGTGGGTCGTCGAGCCGCTTCAGGGAGAGTAA

Upstream 100 bases:

>100_bases
TTGCGGCTCGCCAGCATGGCTGACGGTCAAATCCGCTTCATCGGATTGTCGTCCATGTTGCTCGGGCTCGTTCTCCTGTT
CGTTTTCAACTGAGTCTTAC

Downstream 100 bases:

>100_bases
GGAATGGCAAAGAACGTAGTGGTCGTCGGCACCCAGTGGGGCGACGAGGGCAAGGGCAAGATCGTCGACTGGCTGACGGA
TCATGCGCGCGGTGTCGTGC

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MRWVLPDHIQDALPSEAASLEALRRRLLDAFRVRGYQLVMPPLLEYLDSLTTGAGQDLKLRTFKLVDQVSGRTMGVRADM
TPQVTRIDAHLLNRAGVSRLCYCGSVLHTLPSTLTATREPLQLGAELYGHAGIDADIEIVRLLADVLRLAEVPASRIDIG
HVGLFHALAALAGMVPEREEELFDLLQAKDVPGLKEITVGVAEPVRTALLRLPALYGGAEVIDEAAACMPESAEIRAALD
DLRRLAAALEDLPISFDLADLRGYHYHSGVVFAAYGGGSPAALALGGRYDRVGEAFGRARPATGFSLDLRELALRLPAAV
VPGAILAPLEGTAGLAAAVEALRAAGEAVMSRLPGHEGTWNDAGCDRQLVMRQGAWVVEPLQGE

Sequences:

>Translated_384_residues
MRWVLPDHIQDALPSEAASLEALRRRLLDAFRVRGYQLVMPPLLEYLDSLTTGAGQDLKLRTFKLVDQVSGRTMGVRADM
TPQVTRIDAHLLNRAGVSRLCYCGSVLHTLPSTLTATREPLQLGAELYGHAGIDADIEIVRLLADVLRLAEVPASRIDIG
HVGLFHALAALAGMVPEREEELFDLLQAKDVPGLKEITVGVAEPVRTALLRLPALYGGAEVIDEAAACMPESAEIRAALD
DLRRLAAALEDLPISFDLADLRGYHYHSGVVFAAYGGGSPAALALGGRYDRVGEAFGRARPATGFSLDLRELALRLPAAV
VPGAILAPLEGTAGLAAAVEALRAAGEAVMSRLPGHEGTWNDAGCDRQLVMRQGAWVVEPLQGE
>Mature_384_residues
MRWVLPDHIQDALPSEAASLEALRRRLLDAFRVRGYQLVMPPLLEYLDSLTTGAGQDLKLRTFKLVDQVSGRTMGVRADM
TPQVTRIDAHLLNRAGVSRLCYCGSVLHTLPSTLTATREPLQLGAELYGHAGIDADIEIVRLLADVLRLAEVPASRIDIG
HVGLFHALAALAGMVPEREEELFDLLQAKDVPGLKEITVGVAEPVRTALLRLPALYGGAEVIDEAAACMPESAEIRAALD
DLRRLAAALEDLPISFDLADLRGYHYHSGVVFAAYGGGSPAALALGGRYDRVGEAFGRARPATGFSLDLRELALRLPAAV
VPGAILAPLEGTAGLAAAVEALRAAGEAVMSRLPGHEGTWNDAGCDRQLVMRQGAWVVEPLQGE

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISZ_AZOSB (A1K3Z9)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932441.1
- ProteinModelPortal:   A1K3Z9
- SMR:   A1K3Z9
- STRING:   A1K3Z9
- GeneID:   4606636
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo0937
- eggNOG:   COG3705
- HOGENOM:   HBG616575
- OMA:   DLRGYHY
- ProtClustDB:   PRK12421
- BioCyc:   ASP62928:AZO0937-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00125
- InterPro:   IPR004517
- InterPro:   IPR004516
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00443

Pfam domain/function: NA

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 41076; Mature: 41076

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWVLPDHIQDALPSEAASLEALRRRLLDAFRVRGYQLVMPPLLEYLDSLTTGAGQDLKL
CCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHCCCCCCCCCH
RTFKLVDQVSGRTMGVRADMTPQVTRIDAHLLNRAGVSRLCYCGSVLHTLPSTLTATREP
HHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQLGAELYGHAGIDADIEIVRLLADVLRLAEVPASRIDIGHVGLFHALAALAGMVPEREE
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHCCCCCHHH
ELFDLLQAKDVPGLKEITVGVAEPVRTALLRLPALYGGAEVIDEAAACMPESAEIRAALD
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHH
DLRRLAAALEDLPISFDLADLRGYHYHSGVVFAAYGGGSPAALALGGRYDRVGEAFGRAR
HHHHHHHHHHHCCCCEEHHHHCCEEEECCEEEEEECCCCCCEEEECCCHHHHHHHHHCCC
PATGFSLDLRELALRLPAAVVPGAILAPLEGTAGLAAAVEALRAAGEAVMSRLPGHEGTW
CCCCCCHHHHHHHHHCCHHHCCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
NDAGCDRQLVMRQGAWVVEPLQGE
CCCCCCHHHHHHCCCEEECCCCCC
>Mature Secondary Structure
MRWVLPDHIQDALPSEAASLEALRRRLLDAFRVRGYQLVMPPLLEYLDSLTTGAGQDLKL
CCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHCCCCCCCCCH
RTFKLVDQVSGRTMGVRADMTPQVTRIDAHLLNRAGVSRLCYCGSVLHTLPSTLTATREP
HHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQLGAELYGHAGIDADIEIVRLLADVLRLAEVPASRIDIGHVGLFHALAALAGMVPEREE
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHCCCCCHHH
ELFDLLQAKDVPGLKEITVGVAEPVRTALLRLPALYGGAEVIDEAAACMPESAEIRAALD
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHH
DLRRLAAALEDLPISFDLADLRGYHYHSGVVFAAYGGGSPAALALGGRYDRVGEAFGRAR
HHHHHHHHHHHCCCCEEHHHHCCEEEECCEEEEEECCCCCCEEEECCCHHHHHHHHHCCC
PATGFSLDLRELALRLPAAVVPGAILAPLEGTAGLAAAVEALRAAGEAVMSRLPGHEGTW
CCCCCCHHHHHHHHHCCHHHCCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
NDAGCDRQLVMRQGAWVVEPLQGE
CCCCCCHHHHHHCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA