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The map label for this gene is mpl [H]

Identifier: 119897159

GI number: 119897159

Start: 929650

End: 930996

Strand: Direct

Name: mpl [H]

Synonym: azo0868

Alternate gene names: 119897159

Gene position: 929650-930996 (Clockwise)

Preceding gene: 119897149

Following gene: 119897163

Centisome position: 21.24

GC content: 68.52

Gene sequence:

>1347_bases
ATGCACATCCACATTCTCGGTATCTGCGGCACCTTCATGGGCGGGGTGGCACTGCTCGCGCGCGCGGCCGGGCACACCGT
TACCGGCTGCGACAGCAACGTCTATCCGCCGATGAGCACCCAGCTCGAGGAGCAGGGCATCGCGCTCAGCGAGGGCTACG
ACCCCGCTCAGCTGGATCTTGCGCCCGACGTCTTCGTCATCGGCAACGCGATTTCCCGCGGCAATCCGCTGCTGGAGGCG
ATTCTCGACCGCGGTCTGCCCTATGTCTCCGGTCCGCAATGGCTGGCCGAGCACGTGCTGGCGGGGCGCTGGGTGCTTGC
CGTCGCGGGGACTCACGGCAAGACGACCACGACCTCGCTGCTGGCCTGGATGCTGGAGGACGCAGGCTTGTCGCCGGGCT
TTCTGGTCGGCGGCGTGCCGAAGAACTTCGGGGTGTCGGCGCGCCTGACCGATTCGCCCTTCTTCGTCATCGAGGCCGAC
GAGTACGACACCGCCTTCTGCGACAAGCGTTCGAAGTTCGTTCATTACCGGCCGCGCACCGCAATCCTCAATAACCTGGA
GTTCGACCACGCCGACATCTTTGCCGATCTGGCCGCGATCGAAACGCAGTTTCATCACCTCGTCCGCACGCTGCCGCGGC
AGGGGCGCATCGTCGCCAATGCGCGCGAGGATGCCCTGAAGCGGGTGCTGGCGCGCGGTTGCTGGTCCGAACTGGAATGG
TTCAACGACGCGGCGCAGTGGTCGGCCGCCGCAGGTGCGCGCGATGCCGAGGCGGTGTTCCGGCTGGGGGATGAAGAGCT
GGGGCGGGTGGAGATGCCGCTTGCCGGCAGCCACAACCGCGAGAATGCACTGGCGGCGATCGCGGCGGCGCGTCACGTGG
GTGTGCCGCCGCAACAGGCGATCGCGAGCCTTGCCCGGTTCGAGGGCATCAAGCGGCGGCTGGAGCTGCGGGGTCGCGCG
CGTGGGGTTGCGGTGTATGACGACTTCGCCCATCACCCCACCGCAATCGCGCTTACCGTTGAAGGCTTGCGTCGGCGCGA
GCCGGAAGGCCGCATCCTGGCGGTGCTCGAGCCGCGCTCGAACACCATGAAGCTGGGTGTGATGAAGGCTCAGTTGCCCG
GGAGTCTCGCGCAGGCGGACGCGGTGTTCTGCTACGCCGGCGGGTTGGGCTGGGATGCGGCCGCCGCGCTGGCGCCGCTG
GGTGAGCGCGCGCAGGTGCATGAGGACCTCGGCGCGCTGGTGCAGGCGGTGGCCGCGACGGCGCGGCCGGGGGATCATGT
GTTGGTCATGAGCAACGGCGGCTTCGGCGGCGTGCACCAGAAGCTGCTCGACGCGCTCGCCCGCTGA

Upstream 100 bases:

>100_bases
GGTCGCGGCGGAGGCGGCGGCGCCATCGGTTAGAATGCCGGCCATTCTACACGGGCGGCCTCGCTCCACGAGCCGTCAGC
GGCCGCCTCCCGGAGTTCCC

Downstream 100 bases:

>100_bases
AAGGGGCGGGCGCGTCCCGCCTTAATTGCTGCGCCGGTTCTTGAAGTCGAGGCGGTCCGCCTCGGCCTGGTTGATGTATT
CGAACACCCGCACGACACGC

Product: UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MHIHILGICGTFMGGVALLARAAGHTVTGCDSNVYPPMSTQLEEQGIALSEGYDPAQLDLAPDVFVIGNAISRGNPLLEA
ILDRGLPYVSGPQWLAEHVLAGRWVLAVAGTHGKTTTTSLLAWMLEDAGLSPGFLVGGVPKNFGVSARLTDSPFFVIEAD
EYDTAFCDKRSKFVHYRPRTAILNNLEFDHADIFADLAAIETQFHHLVRTLPRQGRIVANAREDALKRVLARGCWSELEW
FNDAAQWSAAAGARDAEAVFRLGDEELGRVEMPLAGSHNRENALAAIAAARHVGVPPQQAIASLARFEGIKRRLELRGRA
RGVAVYDDFAHHPTAIALTVEGLRRREPEGRILAVLEPRSNTMKLGVMKAQLPGSLAQADAVFCYAGGLGWDAAAALAPL
GERAQVHEDLGALVQAVAATARPGDHVLVMSNGGFGGVHQKLLDALAR

Sequences:

>Translated_448_residues
MHIHILGICGTFMGGVALLARAAGHTVTGCDSNVYPPMSTQLEEQGIALSEGYDPAQLDLAPDVFVIGNAISRGNPLLEA
ILDRGLPYVSGPQWLAEHVLAGRWVLAVAGTHGKTTTTSLLAWMLEDAGLSPGFLVGGVPKNFGVSARLTDSPFFVIEAD
EYDTAFCDKRSKFVHYRPRTAILNNLEFDHADIFADLAAIETQFHHLVRTLPRQGRIVANAREDALKRVLARGCWSELEW
FNDAAQWSAAAGARDAEAVFRLGDEELGRVEMPLAGSHNRENALAAIAAARHVGVPPQQAIASLARFEGIKRRLELRGRA
RGVAVYDDFAHHPTAIALTVEGLRRREPEGRILAVLEPRSNTMKLGVMKAQLPGSLAQADAVFCYAGGLGWDAAAALAPL
GERAQVHEDLGALVQAVAATARPGDHVLVMSNGGFGGVHQKLLDALAR
>Mature_448_residues
MHIHILGICGTFMGGVALLARAAGHTVTGCDSNVYPPMSTQLEEQGIALSEGYDPAQLDLAPDVFVIGNAISRGNPLLEA
ILDRGLPYVSGPQWLAEHVLAGRWVLAVAGTHGKTTTTSLLAWMLEDAGLSPGFLVGGVPKNFGVSARLTDSPFFVIEAD
EYDTAFCDKRSKFVHYRPRTAILNNLEFDHADIFADLAAIETQFHHLVRTLPRQGRIVANAREDALKRVLARGCWSELEW
FNDAAQWSAAAGARDAEAVFRLGDEELGRVEMPLAGSHNRENALAAIAAARHVGVPPQQAIASLARFEGIKRRLELRGRA
RGVAVYDDFAHHPTAIALTVEGLRRREPEGRILAVLEPRSNTMKLGVMKAQLPGSLAQADAVFCYAGGLGWDAAAALAPL
GERAQVHEDLGALVQAVAATARPGDHVLVMSNGGFGGVHQKLLDALAR

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=451, Percent_Identity=59.2017738359202, Blast_Score=525, Evalue=1e-150,
Organism=Escherichia coli, GI1786279, Length=394, Percent_Identity=27.4111675126904, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 48036; Mature: 48036

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIHILGICGTFMGGVALLARAAGHTVTGCDSNVYPPMSTQLEEQGIALSEGYDPAQLDL
CEEEEEEHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHCCCEECCCCCCCEECC
APDVFVIGNAISRGNPLLEAILDRGLPYVSGPQWLAEHVLAGRWVLAVAGTHGKTTTTSL
CCCEEEECCHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHH
LAWMLEDAGLSPGFLVGGVPKNFGVSARLTDSPFFVIEADEYDTAFCDKRSKFVHYRPRT
HHHHHHHCCCCCCEEECCCCCCCCCEEEECCCCEEEEECCCCCCHHHCCCCCEEEECCHH
AILNNLEFDHADIFADLAAIETQFHHLVRTLPRQGRIVANAREDALKRVLARGCWSELEW
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHH
FNDAAQWSAAAGARDAEAVFRLGDEELGRVEMPLAGSHNRENALAAIAAARHVGVPPQQA
HHHHHHHHHHCCCCHHHHHHHCCCHHCCEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHH
IASLARFEGIKRRLELRGRARGVAVYDDFAHHPTAIALTVEGLRRREPEGRILAVLEPRS
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEECHHHCCCCCCCEEEEECCCC
NTMKLGVMKAQLPGSLAQADAVFCYAGGLGWDAAAALAPLGERAQVHEDLGALVQAVAAT
CCEEEEEEEECCCCCHHHCCEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
ARPGDHVLVMSNGGFGGVHQKLLDALAR
CCCCCEEEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MHIHILGICGTFMGGVALLARAAGHTVTGCDSNVYPPMSTQLEEQGIALSEGYDPAQLDL
CEEEEEEHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHCCCEECCCCCCCEECC
APDVFVIGNAISRGNPLLEAILDRGLPYVSGPQWLAEHVLAGRWVLAVAGTHGKTTTTSL
CCCEEEECCHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHH
LAWMLEDAGLSPGFLVGGVPKNFGVSARLTDSPFFVIEADEYDTAFCDKRSKFVHYRPRT
HHHHHHHCCCCCCEEECCCCCCCCCEEEECCCCEEEEECCCCCCHHHCCCCCEEEECCHH
AILNNLEFDHADIFADLAAIETQFHHLVRTLPRQGRIVANAREDALKRVLARGCWSELEW
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHH
FNDAAQWSAAAGARDAEAVFRLGDEELGRVEMPLAGSHNRENALAAIAAARHVGVPPQQA
HHHHHHHHHHCCCCHHHHHHHCCCHHCCEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHH
IASLARFEGIKRRLELRGRARGVAVYDDFAHHPTAIALTVEGLRRREPEGRILAVLEPRS
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEECHHHCCCCCCCEEEEECCCC
NTMKLGVMKAQLPGSLAQADAVFCYAGGLGWDAAAALAPLGERAQVHEDLGALVQAVAAT
CCEEEEEEEECCCCCHHHCCEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
ARPGDHVLVMSNGGFGGVHQKLLDALAR
CCCCCEEEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]