Click here to switch to the map view.

The map label for this gene is thiC

Identifier: 119897131

GI number: 119897131

Start: 901163

End: 903079

Strand: Reverse

Name: thiC

Synonym: azo0840

Alternate gene names: 119897131

Gene position: 903079-901163 (Counterclockwise)

Preceding gene: 119897132

Following gene: 119897130

Centisome position: 20.64

GC content: 65.36

Gene sequence:

>1917_bases
ATGAACGCCAAGGAACACTTCATCGCCACCGAGGCCAAGGTCGACGAAGCTGCAATCGCCCCGCTGCCCAATTCGCGCAA
GATCTACGTCGAAGGCTCGCGCCCCGACATCCGCGTGCCGATGCGCGAGATCCGCCAGAGCGACACCCCGGCCTCCTTCG
GTGCCGAACCCAATCCGCCCATCTTCGTGTATGACTGTTCCGGCCCCTACACCGACCCGGCCGCGAAGATCGACATCCGC
TCCGGCCTGCCGGCGCTGCGCGCGGGCTGGATCGCGGAGCGTGGCGACACCGAGCAGCTCGCCGACCTCAGCTCCGAGTA
CGGCCGCCAGCGTGCCGCTGACCCCAGGCTGGACGAACTCCGCTTTCCGGGCCTGCACCGCAAGCCGCTGCGCGCCAGGG
CCGGCGCCAATGTCACGCAGATGCACTACGCGCGGCGCGGCATCGTCACGCCGGAAATGGAATACATCGCGATCCGCGAG
AACCTGCGCCGCAAGGACTACCTGGAATCCCTGCTCGCCGCCGGCCCCACCGGACAGAAGATGGCTGCGCTGCTGACCCG
TCAGCATCCGGGCCAGAACTTCGGCGCCGCGATTCCCAACGAAATCACCCCCGAGTTCGTACGCGACGAGGTCGCCCGCG
GCCGCGCCATCATCCCCGCCAACATCAACCACCCGGAAACCGAGCCGATGATCATCGGCCGCAACTTCCTGGTGAAGATC
AACGCCAACATCGGCAACTCGGCGCTCGGCTCCTCCATCGCCGAGGAAGTCGACAAGATGACCTGGTCCATCCGCTGGGG
CGGCGACACCGTGATGGACCTCTCGACCGGCAAGAACATCCACGAAACGCGCGAGTGGATCATCCGCAACAGCCCCGTGC
CGATCGGCACGGTGCCGATCTACCAGGCGCTGGAAAAGGTCGACGGCAAGGCCGAGGACCTCACCTGGGAGATCTTCCGC
GACACCCTGATCGAGCAGGCCGAACAGGGCGTGGACTACTTCACCATCCACGCGGGCGTGCTGCTGCGCTACATCCCGCT
CACCGCCAACCGCATGACCGGCATCGTCAGCCGCGGCGGCTCCATCATGGCGAAGTGGTGCCTGGCGCATCACAAGGAGA
GCTTCCTCTACACCCACTTCGAGGACATCTGCGAAATCATGAAGGCCTACGACGTGGCCTTCTCGCTCGGTGACGGCCTG
CGCCCCGGCTCCATCTACGACGCCAACGACGAAGCCCAGCTCGGCGAACTCAAGACCCTGGGCGAACTCACCGACATCGC
CTGGAAGCACGACGTGCAGGTGATGATCGAAGGCCCGGGCCATGTGCCGCTGCACATGATCAAGGAGAACATGGATCTCC
AGCTCGAGCAGTGCAAGGAAGCGCCCTTCTACACCCTGGGGCCGCTCACCACCGACATCGCGCCGGGCTACGACCACATC
ACCAGCGGCATCGGCGCCGCCACCATCGGCTGGTACGGCACCGCGATGCTGTGCTACGTGACGCCCAAGGAGCATCTGGG
CCTGCCCAACAAGCAGGACGTCAAGGAAGGCATCATCACCTACAAGCTCGCCGCCCACGCCGCGGACCTCGCCAAGGGCC
ACCCGGGCGCGCAGATCCGCGACAACGCGCTGTCGAAGGCACGCTTCGAGTTCCGCTGGGAAGACCAGTTCAACCTCGGC
CTCGATCCGGACAAGGCCAAGGAATTCCACGATGAGACCCTGCCCAAGGAATCGGCCAAGGTCGCGCACTTCTGCTCGAT
GTGCGGGCCGCACTTCTGCTCGATGAAGATCACCCAGGACGTGCGCGACTTCGCCGCCCAGCAAGGGGTGGACGAAGCCG
AGGCCCTGCAGAAGGGCATGGAGGTGAAGGCGGTGGAGTTCGTCAAGAGCGGCGCCGAGGTCTATCGCAACGTGTAA

Upstream 100 bases:

>100_bases
GGCGACGCTGAGGCGGTCGCGCCGTCCGTCCACATCCGCACATTCCACAAGAAGCAGTCCTCAGCCTGCCTGCTCCATAC
CCGTACCCCAGGAGACCCCC

Downstream 100 bases:

>100_bases
GCAGTCCCGTCCGGGTTGTTCTGCAAACGGGCCGCGTACGCGGCCCGTTGCTTTTACGGCGCCGCTTTTTGGGCGCCGAA
ATGCAACGGCAGCGGCGCGA

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 638; Mature: 638

Protein sequence:

>638_residues
MNAKEHFIATEAKVDEAAIAPLPNSRKIYVEGSRPDIRVPMREIRQSDTPASFGAEPNPPIFVYDCSGPYTDPAAKIDIR
SGLPALRAGWIAERGDTEQLADLSSEYGRQRAADPRLDELRFPGLHRKPLRARAGANVTQMHYARRGIVTPEMEYIAIRE
NLRRKDYLESLLAAGPTGQKMAALLTRQHPGQNFGAAIPNEITPEFVRDEVARGRAIIPANINHPETEPMIIGRNFLVKI
NANIGNSALGSSIAEEVDKMTWSIRWGGDTVMDLSTGKNIHETREWIIRNSPVPIGTVPIYQALEKVDGKAEDLTWEIFR
DTLIEQAEQGVDYFTIHAGVLLRYIPLTANRMTGIVSRGGSIMAKWCLAHHKESFLYTHFEDICEIMKAYDVAFSLGDGL
RPGSIYDANDEAQLGELKTLGELTDIAWKHDVQVMIEGPGHVPLHMIKENMDLQLEQCKEAPFYTLGPLTTDIAPGYDHI
TSGIGAATIGWYGTAMLCYVTPKEHLGLPNKQDVKEGIITYKLAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLG
LDPDKAKEFHDETLPKESAKVAHFCSMCGPHFCSMKITQDVRDFAAQQGVDEAEALQKGMEVKAVEFVKSGAEVYRNV

Sequences:

>Translated_638_residues
MNAKEHFIATEAKVDEAAIAPLPNSRKIYVEGSRPDIRVPMREIRQSDTPASFGAEPNPPIFVYDCSGPYTDPAAKIDIR
SGLPALRAGWIAERGDTEQLADLSSEYGRQRAADPRLDELRFPGLHRKPLRARAGANVTQMHYARRGIVTPEMEYIAIRE
NLRRKDYLESLLAAGPTGQKMAALLTRQHPGQNFGAAIPNEITPEFVRDEVARGRAIIPANINHPETEPMIIGRNFLVKI
NANIGNSALGSSIAEEVDKMTWSIRWGGDTVMDLSTGKNIHETREWIIRNSPVPIGTVPIYQALEKVDGKAEDLTWEIFR
DTLIEQAEQGVDYFTIHAGVLLRYIPLTANRMTGIVSRGGSIMAKWCLAHHKESFLYTHFEDICEIMKAYDVAFSLGDGL
RPGSIYDANDEAQLGELKTLGELTDIAWKHDVQVMIEGPGHVPLHMIKENMDLQLEQCKEAPFYTLGPLTTDIAPGYDHI
TSGIGAATIGWYGTAMLCYVTPKEHLGLPNKQDVKEGIITYKLAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLG
LDPDKAKEFHDETLPKESAKVAHFCSMCGPHFCSMKITQDVRDFAAQQGVDEAEALQKGMEVKAVEFVKSGAEVYRNV
>Mature_638_residues
MNAKEHFIATEAKVDEAAIAPLPNSRKIYVEGSRPDIRVPMREIRQSDTPASFGAEPNPPIFVYDCSGPYTDPAAKIDIR
SGLPALRAGWIAERGDTEQLADLSSEYGRQRAADPRLDELRFPGLHRKPLRARAGANVTQMHYARRGIVTPEMEYIAIRE
NLRRKDYLESLLAAGPTGQKMAALLTRQHPGQNFGAAIPNEITPEFVRDEVARGRAIIPANINHPETEPMIIGRNFLVKI
NANIGNSALGSSIAEEVDKMTWSIRWGGDTVMDLSTGKNIHETREWIIRNSPVPIGTVPIYQALEKVDGKAEDLTWEIFR
DTLIEQAEQGVDYFTIHAGVLLRYIPLTANRMTGIVSRGGSIMAKWCLAHHKESFLYTHFEDICEIMKAYDVAFSLGDGL
RPGSIYDANDEAQLGELKTLGELTDIAWKHDVQVMIEGPGHVPLHMIKENMDLQLEQCKEAPFYTLGPLTTDIAPGYDHI
TSGIGAATIGWYGTAMLCYVTPKEHLGLPNKQDVKEGIITYKLAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLG
LDPDKAKEFHDETLPKESAKVAHFCSMCGPHFCSMKITQDVRDFAAQQGVDEAEALQKGMEVKAVEFVKSGAEVYRNV

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=638, Percent_Identity=68.3385579937304, Blast_Score=897, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_AZOSB (A1K3Q2)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932344.1
- STRING:   A1K3Q2
- GeneID:   4606603
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo0840
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- PhylomeDB:   A1K3Q2
- ProtClustDB:   PRK09284
- BioCyc:   ASP62928:AZO0840-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 70854; Mature: 70854

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: BINDING 243-243 BINDING 272-272 BINDING 301-301 BINDING 337-337 BINDING 437-437 BINDING 464-464

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAKEHFIATEAKVDEAAIAPLPNSRKIYVEGSRPDIRVPMREIRQSDTPASFGAEPNPP
CCCCCCEEEECCCCCCHHCCCCCCCCEEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCC
IFVYDCSGPYTDPAAKIDIRSGLPALRAGWIAERGDTEQLADLSSEYGRQRAADPRLDEL
EEEEECCCCCCCCCCEEECCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHC
RFPGLHRKPLRARAGANVTQMHYARRGIVTPEMEYIAIRENLRRKDYLESLLAAGPTGQK
CCCCCCCCCCHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
MAALLTRQHPGQNFGAAIPNEITPEFVRDEVARGRAIIPANINHPETEPMIIGRNFLVKI
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEECCEEEEEE
NANIGNSALGSSIAEEVDKMTWSIRWGGDTVMDLSTGKNIHETREWIIRNSPVPIGTVPI
ECCCCCHHHHHHHHHHHHHHEEEEEECCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCHH
YQALEKVDGKAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVLLRYIPLTANRMTGIVSRGG
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCHHHHHHHHHCCC
SIMAKWCLAHHKESFLYTHFEDICEIMKAYDVAFSLGDGLRPGSIYDANDEAQLGELKTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHH
GELTDIAWKHDVQVMIEGPGHVPLHMIKENMDLQLEQCKEAPFYTLGPLTTDIAPGYDHI
HHHHHHHCCCCEEEEEECCCCCCHHHHHCCCCCCHHHHCCCCCEEECCCCCCCCCCHHHH
TSGIGAATIGWYGTAMLCYVTPKEHLGLPNKQDVKEGIITYKLAAHAADLAKGHPGAQIR
HCCCCHHHHHHHCEEEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
DNALSKARFEFRWEDQFNLGLDPDKAKEFHDETLPKESAKVAHFCSMCGPHFCSMKITQD
HHHHHHCEEEEEECCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHCCCHHHHH
VRDFAAQQGVDEAEALQKGMEVKAVEFVKSGAEVYRNV
HHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNAKEHFIATEAKVDEAAIAPLPNSRKIYVEGSRPDIRVPMREIRQSDTPASFGAEPNPP
CCCCCCEEEECCCCCCHHCCCCCCCCEEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCC
IFVYDCSGPYTDPAAKIDIRSGLPALRAGWIAERGDTEQLADLSSEYGRQRAADPRLDEL
EEEEECCCCCCCCCCEEECCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHC
RFPGLHRKPLRARAGANVTQMHYARRGIVTPEMEYIAIRENLRRKDYLESLLAAGPTGQK
CCCCCCCCCCHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
MAALLTRQHPGQNFGAAIPNEITPEFVRDEVARGRAIIPANINHPETEPMIIGRNFLVKI
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEECCEEEEEE
NANIGNSALGSSIAEEVDKMTWSIRWGGDTVMDLSTGKNIHETREWIIRNSPVPIGTVPI
ECCCCCHHHHHHHHHHHHHHEEEEEECCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCHH
YQALEKVDGKAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVLLRYIPLTANRMTGIVSRGG
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCHHHHHHHHHCCC
SIMAKWCLAHHKESFLYTHFEDICEIMKAYDVAFSLGDGLRPGSIYDANDEAQLGELKTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHH
GELTDIAWKHDVQVMIEGPGHVPLHMIKENMDLQLEQCKEAPFYTLGPLTTDIAPGYDHI
HHHHHHHCCCCEEEEEECCCCCCHHHHHCCCCCCHHHHCCCCCEEECCCCCCCCCCHHHH
TSGIGAATIGWYGTAMLCYVTPKEHLGLPNKQDVKEGIITYKLAAHAADLAKGHPGAQIR
HCCCCHHHHHHHCEEEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
DNALSKARFEFRWEDQFNLGLDPDKAKEFHDETLPKESAKVAHFCSMCGPHFCSMKITQD
HHHHHHCEEEEEECCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHCCCHHHHH
VRDFAAQQGVDEAEALQKGMEVKAVEFVKSGAEVYRNV
HHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA