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The map label for this gene is pckG [H]

Identifier: 119897111

GI number: 119897111

Start: 875655

End: 877499

Strand: Reverse

Name: pckG [H]

Synonym: azo0820

Alternate gene names: 119897111

Gene position: 877499-875655 (Counterclockwise)

Preceding gene: 119897115

Following gene: 119897110

Centisome position: 20.05

GC content: 64.72

Gene sequence:

>1845_bases
ATGAATCAACGCTTTTCCGACGCAGCGATTGCTGCAGCCCCGGCGTATGTCCGCCATGAAGGCCTGAAGAAATGGGTCGC
CGAGATTGCGGCGCTGACCGAACCTGACCGCGTCGTCTGGTGCGACGGCTCGCAGGAGGAATACGACCGGCTGTGCGCCG
AGATGGTCGAATCCGGCATGCTGATCAAGCTCAACCCGGAAAAGCGGAAGAACTCCTACCTCGCCTTCTCCGACCCGTCC
GACGTCGCCCGCGTCGAAGACCGCACCTTCATCTGCTCGAAGGACAAGAACGACGCCGGCCCGACGAACAACTGGGAAGA
CCCGGAAACGATGCGCGGCACGCTGAACGGCCTGTTCAAGGGCGCGATGCACGGCCGCACGATGTACGTGATCCCGTTCT
CGATGGGCCCGCTCGGCAGCCCGATCGCCCACATCGGCGTCGAGATCTCCGACAGCCCCTACGTCGTCACCAACATGCGC
ACCATGACCCGCATGGGCAAGGGCGCGATCGACGTGCTCGGCACCGACGGCGAGTTCGTGCCCTGCGTGCACACCGTCGG
CGCCCCGCTGGCCCATGGCGAGAAGGACAGCCGCTGGCCGTGCAACCCGACCACCAAGTACATCGTGCATTTCCCCGAAA
CGCGCGAAATCTGGTCCTACGGCTCGGGCTACGGCGGCAACGCGCTGCTCGGCAAGAAGTGCTTCGCGCTGCGCATCGCC
TCCACCATGGCGCGCGACGAAGGCTGGCTGGCCGAACACATGCTGATCCTCGGCGTGGAATCTCCCGAAGGCGAGAAGAC
CTATGTCGCGGCGGCGTTCCCGTCGGCCTGCGGCAAGACCAACTTCGCGATGCTGATCCCGCCGCAGAGCTTCGGCGGCT
GGAAGATCACCACCGTCGGCGACGACATCGCCTGGATCAAGCCGGGCAAGGACGGCAAGTTCTACGCGATCAACCCCGAA
GCCGGCTTCTTCGGCGTGGCCCCGGGCACGTCGGAAAAGACCAACTTCAACGCGATGGCGACGCTGAAGGAAAACGTCAT
CTTCACCAACGTCGCGCTGACGGACGACGGCGACGTGTGGTGGGAAGGCATGAGCAAGGAAGCTCCGGCCCACCTGATCG
ACTGGCAGGGCAAGGATTGGACGCCGGAAATCGCCCGCGAGACCGGCCGCAAGGCAGCCCACCCGAACGCCCGCTTCACC
GCCCCGGCCAGCCAGTGCCCGTCGATCGACGCCGCGTGGGAAAGCCCGGAAGGCGTGCCGATCTCGGCCTTCATCTTCGG
CGGCCGCCGCGCCACCACCGTGCCGCTGGTGTACCAGGCTTTCAACTGGAACTTCGGCGTCTACATGGCTGCCACCCTGG
GTTCGGAAACCACCGCCGCCGCCTTCGGCGCCCAGGGCGTGGTGCGCCGCGACCCGTTCGCGATGCTGCCGTTCTGCGGC
TACCACATGGGCGACTACTTCAACCACTGGCTGAAGATGGGTCACGTGGTCGAGCAGACGCCGAAGATCTTCTGCGTGAA
CTGGTTCCGCATGAACGAAGAAGGCCAGTTCATGTGGCCGGGCTTCGGCGAGAACATGCGCGTGCTGAAGTGGATCGTCG
ACCGCGTCAAGGGCCGCGTCGCCGCCAAGGAAACCACGCTGGGCTGGATGCCGAAGTTCGAGGATATCGACTGGAGCGGA
TCCGACGTCACCCGCGAAGAATTCGACGCGCTGACGCAGGTCGATGCCGATGCGTGGAAGTCGGAGCTGGCACTGCACAA
GGAATGGTTCGACAAGCTGCAGGACCGCCTGCCCAAGCAACTGGTGCTGAAGCGCGAACTGTTCGAGCTCGCACTCAAGG
TCTGA

Upstream 100 bases:

>100_bases
GACCGCACACGGGATGCCGCGCGGTCAAACGGCCTGTAGCAACGGGGCCGCGGACGGAAAAACCGCATCCGCAAATCAGG
AGAGAGGAGAGCTCCAAGCC

Downstream 100 bases:

>100_bases
GCATCGCGGCTTCGCGCCGCACTGCTCGCGAAAGCGCCGCCCCGCGGCGCTTTTTTCATTTTCGGCCTCCGGTTTTGGGC
TAGCCTGAACCCTTCCGCTA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 614; Mature: 614

Protein sequence:

>614_residues
MNQRFSDAAIAAAPAYVRHEGLKKWVAEIAALTEPDRVVWCDGSQEEYDRLCAEMVESGMLIKLNPEKRKNSYLAFSDPS
DVARVEDRTFICSKDKNDAGPTNNWEDPETMRGTLNGLFKGAMHGRTMYVIPFSMGPLGSPIAHIGVEISDSPYVVTNMR
TMTRMGKGAIDVLGTDGEFVPCVHTVGAPLAHGEKDSRWPCNPTTKYIVHFPETREIWSYGSGYGGNALLGKKCFALRIA
STMARDEGWLAEHMLILGVESPEGEKTYVAAAFPSACGKTNFAMLIPPQSFGGWKITTVGDDIAWIKPGKDGKFYAINPE
AGFFGVAPGTSEKTNFNAMATLKENVIFTNVALTDDGDVWWEGMSKEAPAHLIDWQGKDWTPEIARETGRKAAHPNARFT
APASQCPSIDAAWESPEGVPISAFIFGGRRATTVPLVYQAFNWNFGVYMAATLGSETTAAAFGAQGVVRRDPFAMLPFCG
YHMGDYFNHWLKMGHVVEQTPKIFCVNWFRMNEEGQFMWPGFGENMRVLKWIVDRVKGRVAAKETTLGWMPKFEDIDWSG
SDVTREEFDALTQVDADAWKSELALHKEWFDKLQDRLPKQLVLKRELFELALKV

Sequences:

>Translated_614_residues
MNQRFSDAAIAAAPAYVRHEGLKKWVAEIAALTEPDRVVWCDGSQEEYDRLCAEMVESGMLIKLNPEKRKNSYLAFSDPS
DVARVEDRTFICSKDKNDAGPTNNWEDPETMRGTLNGLFKGAMHGRTMYVIPFSMGPLGSPIAHIGVEISDSPYVVTNMR
TMTRMGKGAIDVLGTDGEFVPCVHTVGAPLAHGEKDSRWPCNPTTKYIVHFPETREIWSYGSGYGGNALLGKKCFALRIA
STMARDEGWLAEHMLILGVESPEGEKTYVAAAFPSACGKTNFAMLIPPQSFGGWKITTVGDDIAWIKPGKDGKFYAINPE
AGFFGVAPGTSEKTNFNAMATLKENVIFTNVALTDDGDVWWEGMSKEAPAHLIDWQGKDWTPEIARETGRKAAHPNARFT
APASQCPSIDAAWESPEGVPISAFIFGGRRATTVPLVYQAFNWNFGVYMAATLGSETTAAAFGAQGVVRRDPFAMLPFCG
YHMGDYFNHWLKMGHVVEQTPKIFCVNWFRMNEEGQFMWPGFGENMRVLKWIVDRVKGRVAAKETTLGWMPKFEDIDWSG
SDVTREEFDALTQVDADAWKSELALHKEWFDKLQDRLPKQLVLKRELFELALKV
>Mature_614_residues
MNQRFSDAAIAAAPAYVRHEGLKKWVAEIAALTEPDRVVWCDGSQEEYDRLCAEMVESGMLIKLNPEKRKNSYLAFSDPS
DVARVEDRTFICSKDKNDAGPTNNWEDPETMRGTLNGLFKGAMHGRTMYVIPFSMGPLGSPIAHIGVEISDSPYVVTNMR
TMTRMGKGAIDVLGTDGEFVPCVHTVGAPLAHGEKDSRWPCNPTTKYIVHFPETREIWSYGSGYGGNALLGKKCFALRIA
STMARDEGWLAEHMLILGVESPEGEKTYVAAAFPSACGKTNFAMLIPPQSFGGWKITTVGDDIAWIKPGKDGKFYAINPE
AGFFGVAPGTSEKTNFNAMATLKENVIFTNVALTDDGDVWWEGMSKEAPAHLIDWQGKDWTPEIARETGRKAAHPNARFT
APASQCPSIDAAWESPEGVPISAFIFGGRRATTVPLVYQAFNWNFGVYMAATLGSETTAAAFGAQGVVRRDPFAMLPFCG
YHMGDYFNHWLKMGHVVEQTPKIFCVNWFRMNEEGQFMWPGFGENMRVLKWIVDRVKGRVAAKETTLGWMPKFEDIDWSG
SDVTREEFDALTQVDADAWKSELALHKEWFDKLQDRLPKQLVLKRELFELALKV

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle [H]

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family [H]

Homologues:

Organism=Homo sapiens, GI187281517, Length=608, Percent_Identity=50.1644736842105, Blast_Score=604, Evalue=1e-173,
Organism=Homo sapiens, GI66346721, Length=603, Percent_Identity=50.5804311774461, Blast_Score=592, Evalue=1e-169,
Organism=Homo sapiens, GI66346723, Length=387, Percent_Identity=52.1963824289406, Blast_Score=392, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17555492, Length=618, Percent_Identity=49.0291262135922, Blast_Score=577, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI32564411, Length=557, Percent_Identity=51.5260323159785, Blast_Score=562, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI71989645, Length=604, Percent_Identity=48.1788079470199, Blast_Score=555, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI71989658, Length=604, Percent_Identity=48.1788079470199, Blast_Score=555, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI71989653, Length=580, Percent_Identity=49.4827586206897, Blast_Score=545, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI32564407, Length=527, Percent_Identity=50.853889943074, Blast_Score=518, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI71989649, Length=504, Percent_Identity=50.7936507936508, Blast_Score=493, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI32564409, Length=373, Percent_Identity=48.2573726541555, Blast_Score=337, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI17540956, Length=512, Percent_Identity=26.7578125, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24655085, Length=606, Percent_Identity=49.6699669966997, Blast_Score=576, Evalue=1e-164,
Organism=Drosophila melanogaster, GI24655082, Length=619, Percent_Identity=48.626817447496, Blast_Score=574, Evalue=1e-164,
Organism=Drosophila melanogaster, GI161077226, Length=573, Percent_Identity=50.261780104712, Blast_Score=551, Evalue=1e-157,
Organism=Drosophila melanogaster, GI24655088, Length=507, Percent_Identity=51.8737672583826, Blast_Score=508, Evalue=1e-144,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210 [H]

Pfam domain/function: PF00821 PEPCK [H]

EC number: =4.1.1.32 [H]

Molecular weight: Translated: 68344; Mature: 68344

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00505 PEPCK_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQRFSDAAIAAAPAYVRHEGLKKWVAEIAALTEPDRVVWCDGSQEEYDRLCAEMVESGM
CCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCC
LIKLNPEKRKNSYLAFSDPSDVARVEDRTFICSKDKNDAGPTNNWEDPETMRGTLNGLFK
EEEECCCCCCCCEEEECCCCHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
GAMHGRTMYVIPFSMGPLGSPIAHIGVEISDSPYVVTNMRTMTRMGKGAIDVLGTDGEFV
HHHCCCEEEEEEECCCCCCCCHHHEEEEECCCCEEEECHHHHHHCCCCCEEEEECCCCEE
PCVHTVGAPLAHGEKDSRWPCNPTTKYIVHFPETREIWSYGSGYGGNALLGKKCFALRIA
CHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCCCCCCCCCCCHHHHHHHH
STMARDEGWLAEHMLILGVESPEGEKTYVAAAFPSACGKTNFAMLIPPQSFGGWKITTVG
HHHHCCCCHHEEEEEEEEECCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCEEEEEEC
DDIAWIKPGKDGKFYAINPEAGFFGVAPGTSEKTNFNAMATLKENVIFTNVALTDDGDVW
CCEEEEECCCCCCEEEECCCCCEEECCCCCCCCCCCCEEEEEECCEEEEEEEEECCCCCE
WEGMSKEAPAHLIDWQGKDWTPEIARETGRKAAHPNARFTAPASQCPSIDAAWESPEGVP
ECCCCCCCCCEEEECCCCCCCHHHHHHCCCCCCCCCCEEECCHHHCCCCCCCCCCCCCCE
ISAFIFGGRRATTVPLVYQAFNWNFGVYMAATLGSETTAAAFGAQGVVRRDPFAMLPFCG
EEEEEECCCCCEEEEEEEEEECCCCCEEEEEECCCCCHHHHHCCCCCCCCCCCEECCHHH
YHMGDYFNHWLKMGHVVEQTPKIFCVNWFRMNEEGQFMWPGFGENMRVLKWIVDRVKGRV
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHCCE
AAKETTLGWMPKFEDIDWSGSDVTREEFDALTQVDADAWKSELALHKEWFDKLQDRLPKQ
EECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHH
LVLKRELFELALKV
HHHHHHHHHHHHCC
>Mature Secondary Structure
MNQRFSDAAIAAAPAYVRHEGLKKWVAEIAALTEPDRVVWCDGSQEEYDRLCAEMVESGM
CCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCC
LIKLNPEKRKNSYLAFSDPSDVARVEDRTFICSKDKNDAGPTNNWEDPETMRGTLNGLFK
EEEECCCCCCCCEEEECCCCHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
GAMHGRTMYVIPFSMGPLGSPIAHIGVEISDSPYVVTNMRTMTRMGKGAIDVLGTDGEFV
HHHCCCEEEEEEECCCCCCCCHHHEEEEECCCCEEEECHHHHHHCCCCCEEEEECCCCEE
PCVHTVGAPLAHGEKDSRWPCNPTTKYIVHFPETREIWSYGSGYGGNALLGKKCFALRIA
CHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCCCCCCCCCCCHHHHHHHH
STMARDEGWLAEHMLILGVESPEGEKTYVAAAFPSACGKTNFAMLIPPQSFGGWKITTVG
HHHHCCCCHHEEEEEEEEECCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCEEEEEEC
DDIAWIKPGKDGKFYAINPEAGFFGVAPGTSEKTNFNAMATLKENVIFTNVALTDDGDVW
CCEEEEECCCCCCEEEECCCCCEEECCCCCCCCCCCCEEEEEECCEEEEEEEEECCCCCE
WEGMSKEAPAHLIDWQGKDWTPEIARETGRKAAHPNARFTAPASQCPSIDAAWESPEGVP
ECCCCCCCCCEEEECCCCCCCHHHHHHCCCCCCCCCCEEECCHHHCCCCCCCCCCCCCCE
ISAFIFGGRRATTVPLVYQAFNWNFGVYMAATLGSETTAAAFGAQGVVRRDPFAMLPFCG
EEEEEECCCCCEEEEEEEEEECCCCCEEEEEECCCCCHHHHHCCCCCCCCCCCEECCHHH
YHMGDYFNHWLKMGHVVEQTPKIFCVNWFRMNEEGQFMWPGFGENMRVLKWIVDRVKGRV
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHCCE
AAKETTLGWMPKFEDIDWSGSDVTREEFDALTQVDADAWKSELALHKEWFDKLQDRLPKQ
EECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHH
LVLKRELFELALKV
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA