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The map label for this gene is glyS

Identifier: 119897077

GI number: 119897077

Start: 844007

End: 846094

Strand: Direct

Name: glyS

Synonym: azo0786

Alternate gene names: 119897077

Gene position: 844007-846094 (Clockwise)

Preceding gene: 119897076

Following gene: 119897078

Centisome position: 19.29

GC content: 69.83

Gene sequence:

>2088_bases
ATGACGGCCAACACCCTGCTCGTCGAACTGCTCACCGAGGAACTGCCGCCCAAGGCGCTGCCGCGGCTGGGCGAAACCTT
TGCCACCAAGATCGCCGAGGGCCTCAAGGCGCGCGGCCTCGCCGCCGCCGACGGCGCCTTCCGCTGGTTCGCCAGCCCGC
GCCGCATCGCCATCACCATGGCGGGCGTCGCCGCCGAAGCGGCCGCGAAGGAAGTCACCGAAAAGCTGATGCCGGTCGCG
GTCGCCCTCGACGCTGACGGCAAGCCGACGCCGGCGCTGCTGAAGAAGATGGAAGCCAAGGGCATCCCCGCGAGCGCGGT
CCCTGCCTTCGAGCGCCGCATGGACGGCAAGGCCGAGGCGCTGTTCCACACTGCCGTGGTGCCGGGAGCCAGGCTCGCCG
AGGTGCTTGCCGCCATCGTGCATGATGCGGTCAAGGCACTGCCCATTCCCAAGGTGATGCGCTGGGGCGATGGCGATGCC
ACCTTCGTGCGTCCGGTGCACAAGCTGGTGATGCTGCACGGCGCTGAGGTGGTGCCGGGCCGCGTGCTCGACCTCGATGC
CGGCCGCAGCACCCGCGGCCACCGCTTCATGAGCCGCGGCGAGATCGACATCGCCACCGCCGACGCCTACGAGCCCACGC
TGCTTGCCGAAGGCAAGGTGGTGCCGGACTTCGCCGAGCGCCGCGCCGACATCGAGCGCCAGCTGCTCGCGGAAGCTGCC
CGCCTAGGCGCCCAGCTCGACGACTACACCGACCTGCTCGACGAAGTCACCGCGCTGGTCGAGCACCCGACGGTGTATGT
CGGCGAGTTCGAGGCCGAATTCCTCGCCGTCCCGCAGGAATGCCTGATCCTGACGATGCGCGCCAACCAGAAGTACTTCC
CGCTGTTCGACGCCGCCGGGAAGCTGCAGAACCGCTTCCTGATCGTCTCGAACATGCGCATGGAAGACCCGGTGAACATC
GTCACCGGCAACGCCCGCGTGGTGCGCCCGCGCCTGTCCGACGCGCGCTTCTTCTTCGAGACCGACAAGAAGCACAAGCT
CGATTCGCGCCTGCCGCGGCTGGCCAACGTGGTCTATCACAACAAGCTCGGCAGCGTGCTCGAACGCGTCGAACGGCTCG
AAGCGCTTGCCGGCAAGATCGCCGCGCGCCTGGGTTCGGACGAAGCGGCGGCCAGGCGCGCCGCGCGCTTGGCCAAGGCC
GACCTCGTCTCCGAGATGGTGGGTGAGTTCCCCGAACTGCAGGGCATCATGGGCCGCTACTACGCGTTGCACGATGGCGA
AGGCGCGGTGGTTGCCGACGCGGTGCAGGCCCACTACCAGCCACGCTTCGCCGGCGATGCGCTGCCGGCGGGCAATGTCG
CCGCCGCGGTGGCGCTGGCCGACAAGCTGGACGCCCTGGTCGGCTTCTTCGGCATCGGCATGGTGCCGACCGGCGACAAG
GACCCGTTCGCGCTGCGCCGGGCCGCGCTCGGCGTGCTGCGCATCCTGATGGAAGCGCCCTTGCCGCTCGATCTGGCCGC
GCTGGTGGCCGACGCCGCCGCCGGCTTCAAGCCGGGCCTGCTCACCGCCGACGGCTTCCAGGCCCAGCTGCTCGACTTCA
TGCGCGAACGCCTGAAGAACCTGCTCCGCGAGAGCGGCGGCGAAGTCGCCGCGATCGACGCGGTGCTCGCGCTGGCGCCC
ACCCGCATCGACCTGGTGCCGGCCAAGCTCGCCGCGGTCGAGGCCTTCCGCGCACTGCCCGAAGCCGAGGCGCTGGCCGC
GGCCAACAAGCGCATCGTGAACATCCTGAAGAAGGCCGAAGGCGCGCCGGGTGAGCCCGATGTCGCGCTGCTGCAGGAGG
ACGCCGAGAAGGCGCTGTTCCACCGCGTGATCGAGATCGCGCCGCTGGTGAAGTCCCACGTCGCCAACGAGGACTACACC
GACGCGCTGTGCGTGCTCGCCGGCCTGCGGGCCGAGGTCGACGCCTTCTTCGACGGTGTCATGGTGATGGCCGAGGAGCC
GCTGACGCGGCAGAACCGCCTCGCGCTGCTGGCGCAACTGGCCGGCCTGATGAACCAGGTCGCCGACATTTCCCGCCTGT
CGGCCTGA

Upstream 100 bases:

>100_bases
GCATCCGCAACCTGTCGCGCGCGGTCGCGCAGGCGTATTTCGAATCCCGCGAGGCGCTCGGCTTCCCGATGCTGCCGCAA
CAGAACAAGGAGGCCGCGCA

Downstream 100 bases:

>100_bases
CCGCCACGGCCCGGCGACCGCCGGGCCGGCCTTCCCGTCCGGAGCCCCTGTGAAGCTCATCATTCTCGACCGCGACGGCG
TCATCAACTACGACTCCGAC

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 695; Mature: 694

Protein sequence:

>695_residues
MTANTLLVELLTEELPPKALPRLGETFATKIAEGLKARGLAAADGAFRWFASPRRIAITMAGVAAEAAAKEVTEKLMPVA
VALDADGKPTPALLKKMEAKGIPASAVPAFERRMDGKAEALFHTAVVPGARLAEVLAAIVHDAVKALPIPKVMRWGDGDA
TFVRPVHKLVMLHGAEVVPGRVLDLDAGRSTRGHRFMSRGEIDIATADAYEPTLLAEGKVVPDFAERRADIERQLLAEAA
RLGAQLDDYTDLLDEVTALVEHPTVYVGEFEAEFLAVPQECLILTMRANQKYFPLFDAAGKLQNRFLIVSNMRMEDPVNI
VTGNARVVRPRLSDARFFFETDKKHKLDSRLPRLANVVYHNKLGSVLERVERLEALAGKIAARLGSDEAAARRAARLAKA
DLVSEMVGEFPELQGIMGRYYALHDGEGAVVADAVQAHYQPRFAGDALPAGNVAAAVALADKLDALVGFFGIGMVPTGDK
DPFALRRAALGVLRILMEAPLPLDLAALVADAAAGFKPGLLTADGFQAQLLDFMRERLKNLLRESGGEVAAIDAVLALAP
TRIDLVPAKLAAVEAFRALPEAEALAAANKRIVNILKKAEGAPGEPDVALLQEDAEKALFHRVIEIAPLVKSHVANEDYT
DALCVLAGLRAEVDAFFDGVMVMAEEPLTRQNRLALLAQLAGLMNQVADISRLSA

Sequences:

>Translated_695_residues
MTANTLLVELLTEELPPKALPRLGETFATKIAEGLKARGLAAADGAFRWFASPRRIAITMAGVAAEAAAKEVTEKLMPVA
VALDADGKPTPALLKKMEAKGIPASAVPAFERRMDGKAEALFHTAVVPGARLAEVLAAIVHDAVKALPIPKVMRWGDGDA
TFVRPVHKLVMLHGAEVVPGRVLDLDAGRSTRGHRFMSRGEIDIATADAYEPTLLAEGKVVPDFAERRADIERQLLAEAA
RLGAQLDDYTDLLDEVTALVEHPTVYVGEFEAEFLAVPQECLILTMRANQKYFPLFDAAGKLQNRFLIVSNMRMEDPVNI
VTGNARVVRPRLSDARFFFETDKKHKLDSRLPRLANVVYHNKLGSVLERVERLEALAGKIAARLGSDEAAARRAARLAKA
DLVSEMVGEFPELQGIMGRYYALHDGEGAVVADAVQAHYQPRFAGDALPAGNVAAAVALADKLDALVGFFGIGMVPTGDK
DPFALRRAALGVLRILMEAPLPLDLAALVADAAAGFKPGLLTADGFQAQLLDFMRERLKNLLRESGGEVAAIDAVLALAP
TRIDLVPAKLAAVEAFRALPEAEALAAANKRIVNILKKAEGAPGEPDVALLQEDAEKALFHRVIEIAPLVKSHVANEDYT
DALCVLAGLRAEVDAFFDGVMVMAEEPLTRQNRLALLAQLAGLMNQVADISRLSA
>Mature_694_residues
TANTLLVELLTEELPPKALPRLGETFATKIAEGLKARGLAAADGAFRWFASPRRIAITMAGVAAEAAAKEVTEKLMPVAV
ALDADGKPTPALLKKMEAKGIPASAVPAFERRMDGKAEALFHTAVVPGARLAEVLAAIVHDAVKALPIPKVMRWGDGDAT
FVRPVHKLVMLHGAEVVPGRVLDLDAGRSTRGHRFMSRGEIDIATADAYEPTLLAEGKVVPDFAERRADIERQLLAEAAR
LGAQLDDYTDLLDEVTALVEHPTVYVGEFEAEFLAVPQECLILTMRANQKYFPLFDAAGKLQNRFLIVSNMRMEDPVNIV
TGNARVVRPRLSDARFFFETDKKHKLDSRLPRLANVVYHNKLGSVLERVERLEALAGKIAARLGSDEAAARRAARLAKAD
LVSEMVGEFPELQGIMGRYYALHDGEGAVVADAVQAHYQPRFAGDALPAGNVAAAVALADKLDALVGFFGIGMVPTGDKD
PFALRRAALGVLRILMEAPLPLDLAALVADAAAGFKPGLLTADGFQAQLLDFMRERLKNLLRESGGEVAAIDAVLALAPT
RIDLVPAKLAAVEAFRALPEAEALAAANKRIVNILKKAEGAPGEPDVALLQEDAEKALFHRVIEIAPLVKSHVANEDYTD
ALCVLAGLRAEVDAFFDGVMVMAEEPLTRQNRLALLAQLAGLMNQVADISRLSA

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=695, Percent_Identity=44.8920863309352, Blast_Score=538, Evalue=1e-154,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_AZOSB (A1K3J8)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932290.1
- ProteinModelPortal:   A1K3J8
- STRING:   A1K3J8
- GeneID:   4606566
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo0786
- eggNOG:   COG0751
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- PhylomeDB:   A1K3J8
- ProtClustDB:   PRK01233
- BioCyc:   ASP62928:AZO0786-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- SMART:   SM00836
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 74661; Mature: 74530

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTANTLLVELLTEELPPKALPRLGETFATKIAEGLKARGLAAADGAFRWFASPRRIAITM
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCEEEEEE
AGVAAEAAAKEVTEKLMPVAVALDADGKPTPALLKKMEAKGIPASAVPAFERRMDGKAEA
HHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCHHHH
LFHTAVVPGARLAEVLAAIVHDAVKALPIPKVMRWGDGDATFVRPVHKLVMLHGAEVVPG
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCC
RVLDLDAGRSTRGHRFMSRGEIDIATADAYEPTLLAEGKVVPDFAERRADIERQLLAEAA
EEEEECCCCCCCCCHHHCCCCEEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHH
RLGAQLDDYTDLLDEVTALVEHPTVYVGEFEAEFLAVPQECLILTMRANQKYFPLFDAAG
HHCCCCHHHHHHHHHHHHHHHCCEEEEECCCHHHHHCCHHHEEEEEECCCCCCCHHHHHH
KLQNRFLIVSNMRMEDPVNIVTGNARVVRPRLSDARFFFETDKKHKLDSRLPRLANVVYH
HHCCCEEEEECCCCCCCCEEEECCCEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHH
NKLGSVLERVERLEALAGKIAARLGSDEAAARRAARLAKADLVSEMVGEFPELQGIMGRY
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
YALHDGEGAVVADAVQAHYQPRFAGDALPAGNVAAAVALADKLDALVGFFGIGMVPTGDK
EEEECCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DPFALRRAALGVLRILMEAPLPLDLAALVADAAAGFKPGLLTADGFQAQLLDFMRERLKN
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
LLRESGGEVAAIDAVLALAPTRIDLVPAKLAAVEAFRALPEAEALAAANKRIVNILKKAE
HHHHCCCCEEHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHC
GAPGEPDVALLQEDAEKALFHRVIEIAPLVKSHVANEDYTDALCVLAGLRAEVDAFFDGV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCH
MVMAEEPLTRQNRLALLAQLAGLMNQVADISRLSA
HEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TANTLLVELLTEELPPKALPRLGETFATKIAEGLKARGLAAADGAFRWFASPRRIAITM
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCEEEEEE
AGVAAEAAAKEVTEKLMPVAVALDADGKPTPALLKKMEAKGIPASAVPAFERRMDGKAEA
HHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCHHHH
LFHTAVVPGARLAEVLAAIVHDAVKALPIPKVMRWGDGDATFVRPVHKLVMLHGAEVVPG
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCC
RVLDLDAGRSTRGHRFMSRGEIDIATADAYEPTLLAEGKVVPDFAERRADIERQLLAEAA
EEEEECCCCCCCCCHHHCCCCEEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHH
RLGAQLDDYTDLLDEVTALVEHPTVYVGEFEAEFLAVPQECLILTMRANQKYFPLFDAAG
HHCCCCHHHHHHHHHHHHHHHCCEEEEECCCHHHHHCCHHHEEEEEECCCCCCCHHHHHH
KLQNRFLIVSNMRMEDPVNIVTGNARVVRPRLSDARFFFETDKKHKLDSRLPRLANVVYH
HHCCCEEEEECCCCCCCCEEEECCCEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHH
NKLGSVLERVERLEALAGKIAARLGSDEAAARRAARLAKADLVSEMVGEFPELQGIMGRY
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
YALHDGEGAVVADAVQAHYQPRFAGDALPAGNVAAAVALADKLDALVGFFGIGMVPTGDK
EEEECCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DPFALRRAALGVLRILMEAPLPLDLAALVADAAAGFKPGLLTADGFQAQLLDFMRERLKN
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
LLRESGGEVAAIDAVLALAPTRIDLVPAKLAAVEAFRALPEAEALAAANKRIVNILKKAE
HHHHCCCCEEHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHC
GAPGEPDVALLQEDAEKALFHRVIEIAPLVKSHVANEDYTDALCVLAGLRAEVDAFFDGV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCH
MVMAEEPLTRQNRLALLAQLAGLMNQVADISRLSA
HEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA