| Definition | Azoarcus sp. BH72 chromosome, complete genome. |
|---|---|
| Accession | NC_008702 |
| Length | 4,376,040 |
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The map label for this gene is sinIM [H]
Identifier: 119897062
GI number: 119897062
Start: 824754
End: 826058
Strand: Reverse
Name: sinIM [H]
Synonym: azo0771
Alternate gene names: 119897062
Gene position: 826058-824754 (Counterclockwise)
Preceding gene: 119897063
Following gene: 119897056
Centisome position: 18.88
GC content: 52.57
Gene sequence:
>1305_bases ATGGGCTTAGATCTAGGACTCGACCGCACAGGCCGCTATGAGATAGTTGCCTGTGTCGAGAAAGAGAAAGTCTTCTGCAA TACAATCCGACAAAACTCCGCAGCCGGACGCATTAATCCTAACCTTAAGGTCTTTGAGGGCGACATTAACGACCTCGATC CCGCACAAGTACTAGACTCCGTCAACCTGAAACCCGGAGAAGTTGATCTACTTGTCGGGGGGCCACCGTGCCAATCTTTC AGTACAGCGGGAAAGCGAGGAACGGTTCAAGACCCAAGAGGCACCTTACTTTGGCAATACTTGAGGTTCGTTGAATATAT TCAACCGAAATTTTTCTTAATGGAGAACGTTCGAGGGCTCGTATCAGCTGCGCTTAGGCATCGCCCCATAGCAGAGAGAC CCGAACACGGAGGCGCCCCCCTCACCGACAACGAGAAACCAGGGTCGGTCCTTCGCCGCTTCTCTGAAGACCTGCAAGCC ATTCCAAATGCTGCATACCACTTCGACGTTTTTGAAGTTAACTCGGTTAACTACGGAGCACCGCAGATACGAGAGCGAGT GATATTCATCGGCAATCGGTTCAACATGGAAGTCGATTTCCCCAATCCGACCCACGGCCCTATCGCAGCAGCAAAAGCTC AGGACGACCTCTTCAGCGCTCCCGCAGCGCAGCCTTGGGCAACCCTACGTGATGCAATCGGTGACCTGAACGAGACTAAC CCTGTCGTTCTCGATTTCAGCCCTCGCAAGAAAAGCTTTTTGTCACTCATACCGCCAGGATCAAACTGGAGGAGCTTGCC CGAGAACCTTCAACAGGAATCAATGGGGAAAGCTTGGTTTGCCAAAGGCGGGCGCTCGGGTTGGTGGCGACGCCTCACAT TTGATTTGCCATGTCCAACGCTGGTGACGATGCCTAACCATGCCAGCACGGCCCTATGCCATCCAACAGAAACACGGGCG CTTAGTCTTCGTGAATACGCTCGCATTCAAGAATTCCCCGATGACTGGCAGTTTTCAGGAACAGTATCAGAACAGTATCG CCAGGTTGGCAATGCAGTACCAACAAGATTAGGAAAAATCGCGGGAGAAGTGATCGCTTCATGCCTCGACACCATGAAGG GACGCAACTGGCAGCCTTTCCCGACCCCCCCTGATGCATTCCGTATAATATACATCCAGTCCCACGTCCGCACACGACAG TGGTTCAAAGGCGGAAAGACTGTGATCTGGGAGGATGGCGAACAGAATGAAACCGCTGCGTACGACCGCCCACAGACACT GAGAAAAACGAGCGTAATAGGATAA
Upstream 100 bases:
>100_bases GATAAGCCCTTCGGCCCATTAAGCGCGAAAAATCCATCACCACCTAATGAGCAACAATATCAAACAGAAATTCAAAGTCA TCTCCCTTTTTTCAGGGGGA
Downstream 100 bases:
>100_bases ACGATGGCCAGACGACAAACTGCTTCTGGAAACGGCACCACACTCTCTAACGTTCTCAAAAGCAGAACCCACCATCTAGA TCCAAATGAGGTTATTCGAC
Product: C-5 cytosine-specific DNA methylase
Products: NA
Alternate protein names: M.SinI; Cytosine-specific methyltransferase SinI [H]
Number of amino acids: Translated: 434; Mature: 433
Protein sequence:
>434_residues MGLDLGLDRTGRYEIVACVEKEKVFCNTIRQNSAAGRINPNLKVFEGDINDLDPAQVLDSVNLKPGEVDLLVGGPPCQSF STAGKRGTVQDPRGTLLWQYLRFVEYIQPKFFLMENVRGLVSAALRHRPIAERPEHGGAPLTDNEKPGSVLRRFSEDLQA IPNAAYHFDVFEVNSVNYGAPQIRERVIFIGNRFNMEVDFPNPTHGPIAAAKAQDDLFSAPAAQPWATLRDAIGDLNETN PVVLDFSPRKKSFLSLIPPGSNWRSLPENLQQESMGKAWFAKGGRSGWWRRLTFDLPCPTLVTMPNHASTALCHPTETRA LSLREYARIQEFPDDWQFSGTVSEQYRQVGNAVPTRLGKIAGEVIASCLDTMKGRNWQPFPTPPDAFRIIYIQSHVRTRQ WFKGGKTVIWEDGEQNETAAYDRPQTLRKTSVIG
Sequences:
>Translated_434_residues MGLDLGLDRTGRYEIVACVEKEKVFCNTIRQNSAAGRINPNLKVFEGDINDLDPAQVLDSVNLKPGEVDLLVGGPPCQSF STAGKRGTVQDPRGTLLWQYLRFVEYIQPKFFLMENVRGLVSAALRHRPIAERPEHGGAPLTDNEKPGSVLRRFSEDLQA IPNAAYHFDVFEVNSVNYGAPQIRERVIFIGNRFNMEVDFPNPTHGPIAAAKAQDDLFSAPAAQPWATLRDAIGDLNETN PVVLDFSPRKKSFLSLIPPGSNWRSLPENLQQESMGKAWFAKGGRSGWWRRLTFDLPCPTLVTMPNHASTALCHPTETRA LSLREYARIQEFPDDWQFSGTVSEQYRQVGNAVPTRLGKIAGEVIASCLDTMKGRNWQPFPTPPDAFRIIYIQSHVRTRQ WFKGGKTVIWEDGEQNETAAYDRPQTLRKTSVIG >Mature_433_residues GLDLGLDRTGRYEIVACVEKEKVFCNTIRQNSAAGRINPNLKVFEGDINDLDPAQVLDSVNLKPGEVDLLVGGPPCQSFS TAGKRGTVQDPRGTLLWQYLRFVEYIQPKFFLMENVRGLVSAALRHRPIAERPEHGGAPLTDNEKPGSVLRRFSEDLQAI PNAAYHFDVFEVNSVNYGAPQIRERVIFIGNRFNMEVDFPNPTHGPIAAAKAQDDLFSAPAAQPWATLRDAIGDLNETNP VVLDFSPRKKSFLSLIPPGSNWRSLPENLQQESMGKAWFAKGGRSGWWRRLTFDLPCPTLVTMPNHASTALCHPTETRAL SLREYARIQEFPDDWQFSGTVSEQYRQVGNAVPTRLGKIAGEVIASCLDTMKGRNWQPFPTPPDAFRIIYIQSHVRTRQW FKGGKTVIWEDGEQNETAAYDRPQTLRKTSVIG
Specific function: This methylase recognizes the double-stranded sequence GGWCC, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the SinI endonuclease [H]
COG id: COG0270
COG function: function code L; Site-specific DNA methylase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the C5-methyltransferase family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR018117 - InterPro: IPR001525 - InterPro: IPR010093 [H]
Pfam domain/function: PF00145 DNA_methylase [H]
EC number: =2.1.1.37 [H]
Molecular weight: Translated: 48621; Mature: 48489
Theoretical pI: Translated: 7.85; Mature: 7.85
Prosite motif: PS00094 C5_MTASE_1
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.4 %Cys (Translated Protein) 1.4 %Met (Translated Protein) 2.8 %Cys+Met (Translated Protein) 1.4 %Cys (Mature Protein) 1.2 %Met (Mature Protein) 2.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MGLDLGLDRTGRYEIVACVEKEKVFCNTIRQNSAAGRINPNLKVFEGDINDLDPAQVLDS CCCCCCCCCCCCEEEEEEECHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCHHHHHHC VNLKPGEVDLLVGGPPCQSFSTAGKRGTVQDPRGTLLWQYLRFVEYIQPKFFLMENVRGL CCCCCCCEEEEECCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHH VSAALRHRPIAERPEHGGAPLTDNEKPGSVLRRFSEDLQAIPNAAYHFDVFEVNSVNYGA HHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCC PQIRERVIFIGNRFNMEVDFPNPTHGPIAAAKAQDDLFSAPAAQPWATLRDAIGDLNETN HHHCCEEEEEECEEEEEECCCCCCCCCEEHHHCCHHHHCCCCCCHHHHHHHHHCCCCCCC PVVLDFSPRKKSFLSLIPPGSNWRSLPENLQQESMGKAWFAKGGRSGWWRRLTFDLPCPT CEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEEECCCCCE LVTMPNHASTALCHPTETRALSLREYARIQEFPDDWQFSGTVSEQYRQVGNAVPTRLGKI EEECCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH AGEVIASCLDTMKGRNWQPFPTPPDAFRIIYIQSHVRTRQWFKGGKTVIWEDGEQNETAA HHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECHHHHHHHHCCCCEEEECCCCCCCCCC YDRPQTLRKTSVIG CCCCHHHHHHCCCC >Mature Secondary Structure GLDLGLDRTGRYEIVACVEKEKVFCNTIRQNSAAGRINPNLKVFEGDINDLDPAQVLDS CCCCCCCCCCCEEEEEEECHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCHHHHHHC VNLKPGEVDLLVGGPPCQSFSTAGKRGTVQDPRGTLLWQYLRFVEYIQPKFFLMENVRGL CCCCCCCEEEEECCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHH VSAALRHRPIAERPEHGGAPLTDNEKPGSVLRRFSEDLQAIPNAAYHFDVFEVNSVNYGA HHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCC PQIRERVIFIGNRFNMEVDFPNPTHGPIAAAKAQDDLFSAPAAQPWATLRDAIGDLNETN HHHCCEEEEEECEEEEEECCCCCCCCCEEHHHCCHHHHCCCCCCHHHHHHHHHCCCCCCC PVVLDFSPRKKSFLSLIPPGSNWRSLPENLQQESMGKAWFAKGGRSGWWRRLTFDLPCPT CEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEEECCCCCE LVTMPNHASTALCHPTETRALSLREYARIQEFPDDWQFSGTVSEQYRQVGNAVPTRLGKI EEECCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH AGEVIASCLDTMKGRNWQPFPTPPDAFRIIYIQSHVRTRQWFKGGKTVIWEDGEQNETAA HHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECHHHHHHHHCCCCEEEECCCCCCCCCC YDRPQTLRKTSVIG CCCCHHHHHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 2836359 [H]