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The map label for this gene is ipk

Identifier: 119897047

GI number: 119897047

Start: 807698

End: 808546

Strand: Reverse

Name: ipk

Synonym: azo0756

Alternate gene names: 119897047

Gene position: 808546-807698 (Counterclockwise)

Preceding gene: 119897048

Following gene: 119897046

Centisome position: 18.48

GC content: 65.37

Gene sequence:

>849_bases
ATGACCATCAGCGGTGTCCTCGCCGGCTGCCCCGCGCCGGCCAAGCTGAACCTGTTCCTGCATGTCGTCGGACGCCGCGA
CGACGGCTACCACCTGCTGCAGACCGCGTTCCGGCTGCTCGACTGGGGCGACCGCCTGGACTTCCGCGTGCGCGACGACG
GTCTCATCCGCCGCACCAACCAGGTGGCCGGCGTCGCCGAGGACGACGATCTGGTCGTGCGCGCCGCGCGCCGCCTGCAG
CAGGCCACAGGCACGCCGCTCGGTGCGGACATCACGCTGCACAAGGTATTACCGATGGGAGGGGGCGTCGGCGGTGGCAG
TTCGGACGCCGCGACCACACTGATTGCGCTCAACCATCTGTGGCAGACCGGCCTCACCCGCGCCGACCTGCAACAGCTCG
GGCTCGCGCTCGGCGCCGACGTTCCGTTCTTCATCTATGGCCGCGACGCCTTTGCCGAGGGCGTGGGCGAAGCCTTCCAG
CCGCTGGCGCTGCCTGCAGCCGTGTATGTGGTGCTGTCACCGGAGGTCAGCGTGCCCACCGCCGAGATTTTTTCAGCAAA
GGGCTTGACTCGGGATACACCCCCAATCAGAATAGCGGACTTCGCAGCGAGCCCTACCCGAAACGACTTACAAGCAACGG
CCTGTAGTCGATATCCGGAAGTAGCACGGGCAATAAACTGGCTGGAGCACTACGCGCCAGCGCGGATGACCGGCTCAGGC
GCATGCGTGTTTGCCGAGGTGGCTTCGGAAATCGAAGCAGACGAAATCGTCAGCCTCTGTCCTGCACGCTGGAAAGCCTG
GAAGGCAAAAAGCCTGGCGCGGCATCCGTTGTACGGTTTGCTCGACTAG

Upstream 100 bases:

>100_bases
AGTATCCGGATACGCGCGCCCGCACACTCCCCACCCGGGTAGAAGTCTCCCGCGGCGACGCACGCATCCGGCTGATCATC
GACCAGTGGGAGCTGCTGCA

Downstream 100 bases:

>100_bases
CTGACGAAAAAGGTTTCTTGGGGAGTCGCCAAGTCGGTTAAGGCACCGGATTTTGATTCCGGCATTCGAGGGTTCGAATC
CTTCCTCCCCAGCCAAACGA

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MTISGVLAGCPAPAKLNLFLHVVGRRDDGYHLLQTAFRLLDWGDRLDFRVRDDGLIRRTNQVAGVAEDDDLVVRAARRLQ
QATGTPLGADITLHKVLPMGGGVGGGSSDAATTLIALNHLWQTGLTRADLQQLGLALGADVPFFIYGRDAFAEGVGEAFQ
PLALPAAVYVVLSPEVSVPTAEIFSAKGLTRDTPPIRIADFAASPTRNDLQATACSRYPEVARAINWLEHYAPARMTGSG
ACVFAEVASEIEADEIVSLCPARWKAWKAKSLARHPLYGLLD

Sequences:

>Translated_282_residues
MTISGVLAGCPAPAKLNLFLHVVGRRDDGYHLLQTAFRLLDWGDRLDFRVRDDGLIRRTNQVAGVAEDDDLVVRAARRLQ
QATGTPLGADITLHKVLPMGGGVGGGSSDAATTLIALNHLWQTGLTRADLQQLGLALGADVPFFIYGRDAFAEGVGEAFQ
PLALPAAVYVVLSPEVSVPTAEIFSAKGLTRDTPPIRIADFAASPTRNDLQATACSRYPEVARAINWLEHYAPARMTGSG
ACVFAEVASEIEADEIVSLCPARWKAWKAKSLARHPLYGLLD
>Mature_281_residues
TISGVLAGCPAPAKLNLFLHVVGRRDDGYHLLQTAFRLLDWGDRLDFRVRDDGLIRRTNQVAGVAEDDDLVVRAARRLQQ
ATGTPLGADITLHKVLPMGGGVGGGSSDAATTLIALNHLWQTGLTRADLQQLGLALGADVPFFIYGRDAFAEGVGEAFQP
LALPAAVYVVLSPEVSVPTAEIFSAKGLTRDTPPIRIADFAASPTRNDLQATACSRYPEVARAINWLEHYAPARMTGSGA
CVFAEVASEIEADEIVSLCPARWKAWKAKSLARHPLYGLLD

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=274, Percent_Identity=48.5401459854015, Blast_Score=238, Evalue=2e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_AZOSB (A1K3G8)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932260.1
- ProteinModelPortal:   A1K3G8
- SMR:   A1K3G8
- STRING:   A1K3G8
- GeneID:   4609990
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo0756
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   LNLFLHV
- PhylomeDB:   A1K3G8
- ProtClustDB:   PRK00343
- BioCyc:   ASP62928:AZO0756-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 30255; Mature: 30124

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: ACT_SITE 15-15 ACT_SITE 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISGVLAGCPAPAKLNLFLHVVGRRDDGYHLLQTAFRLLDWGDRLDFRVRDDGLIRRTN
CCCCCEECCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEECCCCHHEEHH
QVAGVAEDDDLVVRAARRLQQATGTPLGADITLHKVLPMGGGVGGGSSDAATTLIALNHL
HHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHH
WQTGLTRADLQQLGLALGADVPFFIYGRDAFAEGVGEAFQPLALPAAVYVVLSPEVSVPT
HHHCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCH
AEIFSAKGLTRDTPPIRIADFAASPTRNDLQATACSRYPEVARAINWLEHYAPARMTGSG
HHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCC
ACVFAEVASEIEADEIVSLCPARWKAWKAKSLARHPLYGLLD
CEEHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TISGVLAGCPAPAKLNLFLHVVGRRDDGYHLLQTAFRLLDWGDRLDFRVRDDGLIRRTN
CCCCEECCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEECCCCHHEEHH
QVAGVAEDDDLVVRAARRLQQATGTPLGADITLHKVLPMGGGVGGGSSDAATTLIALNHL
HHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHH
WQTGLTRADLQQLGLALGADVPFFIYGRDAFAEGVGEAFQPLALPAAVYVVLSPEVSVPT
HHHCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCH
AEIFSAKGLTRDTPPIRIADFAASPTRNDLQATACSRYPEVARAINWLEHYAPARMTGSG
HHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCC
ACVFAEVASEIEADEIVSLCPARWKAWKAKSLARHPLYGLLD
CEEHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA