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The map label for this gene is prsA [H]

Identifier: 119897046

GI number: 119897046

Start: 806573

End: 807523

Strand: Reverse

Name: prsA [H]

Synonym: azo0755

Alternate gene names: 119897046

Gene position: 807523-806573 (Counterclockwise)

Preceding gene: 119897047

Following gene: 119897045

Centisome position: 18.45

GC content: 66.56

Gene sequence:

>951_bases
ATGCCCTACGGCAGCCTGATGGTCTTCACCGGCAACGCCAATCCCAAGCTCGCTGCCGACGTGGCGCGTCGCCTGGGCAT
CTCCCTCGGTTCGGTCACGGTGGGCCGCTTCTCCGACGGCGAGGTCAACGTCGAACTGCTCGAAAACGTCCGCGGCAAGG
ACGTCTTCATCCTGCAGCCGACCTGTGCGCCCACCAACGAAAACCTGATGGAACTGCTGGTGCTGGTCGACGCGCTGAAG
CGCGCCTCCGCCGGCCGCATCACCGCCGCCATCCCCTACTTCGGCTACGCCCGCCAGGACCGCCGCCCGCGCTCGGCGCG
GGTGCCGATCACGGCCAAGGTCGTCGCCAACATGCTGCAGGCCGTGGGCGTGCAGCGCGTGCTGACGATGGACCTGCACG
CCGACCAGATCCAGGGCTTCTTCGACATCCCGGTCGATAACGTCTATGCCGCACCGGTGCTGCTGGCCGACCTCGACAAG
CAGAAGTACGACGACCTGATGGTGGTGTCGCCGGACGTCGGCGGCGTGGTGCGTGCGCGCGCCTTCGCCAAGCGCATGGA
ATGCGACCTGGCGATCATCGACAAGCGCCGCCCGAAGGCCAACGTGTCCGAAGTCATGAACATCATCGGTGAGGTGGACG
GCCGCACCTGCGTGATCATGGACGACATCGTCGATACCGCCGGCACGCTGTGCAAGGCCGCCAGCGCGCTCAAGGAACAC
GGCGCCAAGCGCGTGCTCGCCTACTGTACCCACGCGGTGCTGTCCGGCGCCGCGGTGTCGCGCATCAACGACTCCGAACT
CGACGAACTCGTCGTCACCGACACCATCCCGCTGCGCGACGACGCCAAGGCCAGCTCGCGCATCCGCCAGGTCTCGGTCG
CCTCGCTGCTGGCCGACACGGTGCTGCGGATCAGCAACGAGGAATCGGTTTCCTCGCTGTTCATGGAATAA

Upstream 100 bases:

>100_bases
CGAATCCTTCCTCCCCAGCCAAACGACGACGGGCAGGCCACGGGCCTGCCCGTGTTGTTTTTGACTACGGTTTCATTCTT
TCGGCATCGGAGATTCGGTC

Downstream 100 bases:

>100_bases
GTTTCGAACCGCGGCCCAGCCGCGGTTTTTCCATCTTCGCCTGGTCGCGGGCGAAGGTTCTTCAACCAGGAGTTTTTCAA
CATGACCATCGAATTCAACG

Product: ribose-phosphate diphosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MPYGSLMVFTGNANPKLAADVARRLGISLGSVTVGRFSDGEVNVELLENVRGKDVFILQPTCAPTNENLMELLVLVDALK
RASAGRITAAIPYFGYARQDRRPRSARVPITAKVVANMLQAVGVQRVLTMDLHADQIQGFFDIPVDNVYAAPVLLADLDK
QKYDDLMVVSPDVGGVVRARAFAKRMECDLAIIDKRRPKANVSEVMNIIGEVDGRTCVIMDDIVDTAGTLCKAASALKEH
GAKRVLAYCTHAVLSGAAVSRINDSELDELVVTDTIPLRDDAKASSRIRQVSVASLLADTVLRISNEESVSSLFME

Sequences:

>Translated_316_residues
MPYGSLMVFTGNANPKLAADVARRLGISLGSVTVGRFSDGEVNVELLENVRGKDVFILQPTCAPTNENLMELLVLVDALK
RASAGRITAAIPYFGYARQDRRPRSARVPITAKVVANMLQAVGVQRVLTMDLHADQIQGFFDIPVDNVYAAPVLLADLDK
QKYDDLMVVSPDVGGVVRARAFAKRMECDLAIIDKRRPKANVSEVMNIIGEVDGRTCVIMDDIVDTAGTLCKAASALKEH
GAKRVLAYCTHAVLSGAAVSRINDSELDELVVTDTIPLRDDAKASSRIRQVSVASLLADTVLRISNEESVSSLFME
>Mature_315_residues
PYGSLMVFTGNANPKLAADVARRLGISLGSVTVGRFSDGEVNVELLENVRGKDVFILQPTCAPTNENLMELLVLVDALKR
ASAGRITAAIPYFGYARQDRRPRSARVPITAKVVANMLQAVGVQRVLTMDLHADQIQGFFDIPVDNVYAAPVLLADLDKQ
KYDDLMVVSPDVGGVVRARAFAKRMECDLAIIDKRRPKANVSEVMNIIGEVDGRTCVIMDDIVDTAGTLCKAASALKEHG
AKRVLAYCTHAVLSGAAVSRINDSELDELVVTDTIPLRDDAKASSRIRQVSVASLLADTVLRISNEESVSSLFME

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506127, Length=316, Percent_Identity=46.8354430379747, Blast_Score=298, Evalue=6e-81,
Organism=Homo sapiens, GI4506129, Length=316, Percent_Identity=45.8860759493671, Blast_Score=296, Evalue=2e-80,
Organism=Homo sapiens, GI84875539, Length=318, Percent_Identity=45.2830188679245, Blast_Score=294, Evalue=6e-80,
Organism=Homo sapiens, GI28557709, Length=316, Percent_Identity=45.5696202531646, Blast_Score=287, Evalue=8e-78,
Organism=Homo sapiens, GI4506133, Length=347, Percent_Identity=35.1585014409222, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI194018537, Length=342, Percent_Identity=34.5029239766082, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI310128524, Length=149, Percent_Identity=32.2147651006711, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI310115209, Length=149, Percent_Identity=32.2147651006711, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI310118259, Length=149, Percent_Identity=32.2147651006711, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI310119946, Length=149, Percent_Identity=32.2147651006711, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI1787458, Length=315, Percent_Identity=64.1269841269841, Blast_Score=417, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI25149168, Length=316, Percent_Identity=44.3037974683544, Blast_Score=293, Evalue=7e-80,
Organism=Caenorhabditis elegans, GI17554702, Length=316, Percent_Identity=44.3037974683544, Blast_Score=293, Evalue=8e-80,
Organism=Caenorhabditis elegans, GI17554704, Length=312, Percent_Identity=44.5512820512821, Blast_Score=293, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI71989924, Length=316, Percent_Identity=44.3037974683544, Blast_Score=292, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17570245, Length=338, Percent_Identity=32.8402366863905, Blast_Score=188, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6321776, Length=316, Percent_Identity=44.9367088607595, Blast_Score=270, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6319403, Length=318, Percent_Identity=43.7106918238994, Blast_Score=268, Evalue=6e-73,
Organism=Saccharomyces cerevisiae, GI6320946, Length=319, Percent_Identity=42.9467084639498, Blast_Score=264, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6322667, Length=196, Percent_Identity=41.3265306122449, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6324511, Length=118, Percent_Identity=41.5254237288136, Blast_Score=102, Evalue=8e-23,
Organism=Drosophila melanogaster, GI21355239, Length=316, Percent_Identity=45.8860759493671, Blast_Score=281, Evalue=4e-76,
Organism=Drosophila melanogaster, GI45551540, Length=338, Percent_Identity=42.8994082840237, Blast_Score=271, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24651458, Length=354, Percent_Identity=32.7683615819209, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24651456, Length=354, Percent_Identity=32.7683615819209, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI281362873, Length=355, Percent_Identity=32.9577464788732, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24651454, Length=355, Percent_Identity=32.9577464788732, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24651462, Length=212, Percent_Identity=37.2641509433962, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24651464, Length=212, Percent_Identity=37.2641509433962, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI45552010, Length=213, Percent_Identity=37.5586854460094, Blast_Score=143, Evalue=1e-34,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 34201; Mature: 34070

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYGSLMVFTGNANPKLAADVARRLGISLGSVTVGRFSDGEVNVELLENVRGKDVFILQP
CCCCCEEEEECCCCCHHHHHHHHHHCCCCCCEEEECCCCCCEEHHHHHCCCCCEEEEECC
TCAPTNENLMELLVLVDALKRASAGRITAAIPYFGYARQDRRPRSARVPITAKVVANMLQ
CCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHCCCCCCCCCCCCHHHHHHHHHH
AVGVQRVLTMDLHADQIQGFFDIPVDNVYAAPVLLADLDKQKYDDLMVVSPDVGGVVRAR
HHHHHHHHEECCCHHHHCCEEECCCCCHHHHHHHHHHCCHHHCCCEEEECCCCCHHHHHH
AFAKRMECDLAIIDKRRPKANVSEVMNIIGEVDGRTCVIMDDIVDTAGTLCKAASALKEH
HHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHH
GAKRVLAYCTHAVLSGAAVSRINDSELDELVVTDTIPLRDDAKASSRIRQVSVASLLADT
CHHHHHHHHHHHHHCCHHHHCCCHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
VLRISNEESVSSLFME
HHHCCCHHHHHHHHCC
>Mature Secondary Structure 
PYGSLMVFTGNANPKLAADVARRLGISLGSVTVGRFSDGEVNVELLENVRGKDVFILQP
CCCCEEEEECCCCCHHHHHHHHHHCCCCCCEEEECCCCCCEEHHHHHCCCCCEEEEECC
TCAPTNENLMELLVLVDALKRASAGRITAAIPYFGYARQDRRPRSARVPITAKVVANMLQ
CCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHCCCCCCCCCCCCHHHHHHHHHH
AVGVQRVLTMDLHADQIQGFFDIPVDNVYAAPVLLADLDKQKYDDLMVVSPDVGGVVRAR
HHHHHHHHEECCCHHHHCCEEECCCCCHHHHHHHHHHCCHHHCCCEEEECCCCCHHHHHH
AFAKRMECDLAIIDKRRPKANVSEVMNIIGEVDGRTCVIMDDIVDTAGTLCKAASALKEH
HHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHH
GAKRVLAYCTHAVLSGAAVSRINDSELDELVVTDTIPLRDDAKASSRIRQVSVASLLADT
CHHHHHHHHHHHHHCCHHHHCCCHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
VLRISNEESVSSLFME
HHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]