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The map label for this gene is cls1 [H]

Identifier: 119897034

GI number: 119897034

Start: 793682

End: 794839

Strand: Direct

Name: cls1 [H]

Synonym: azo0743

Alternate gene names: 119897034

Gene position: 793682-794839 (Clockwise)

Preceding gene: 119897033

Following gene: 119897037

Centisome position: 18.14

GC content: 71.07

Gene sequence:

>1158_bases
TTGACGGTGCTGGTCGAAGGCAACGCGATCGCGCTGCTGGAGAACGGTCTGCAGTACTTCCCGGCGCTGGAAGCGGAGAT
CGACAGCGCCCGGCACGAGATCTTCCTCGAAACCTACATCTTCAGCCGTGACGTGGTCGGGCGCCGCATTGCCGCGGCGT
TGGTGCGTGCAGCCGCGCGCGGCGTGCGCGTGCGGGTGCTGGTCGATGGCTTCGGCGGGCGCGAGTTCGTTCGCGACCTG
ATGCCGGGGCTGCTCGCCGACGGCGTCGAGGTGCTGATCTTCCGCCGCGAACTGCGGCTCACCTCGCTGCGGCGCCATCG
GCTGCGGCGCATGCACCGCAAGATCGCGTTGATCGACGCGCGCGTTGCCTTCGTCGGCGGCATCAACATCACCGACGACT
TCGAACTATCCGGCCCACCGCATCCCCGCTACGACTACGCCGTACGGGTGGAGGGACCGCTGCTCGCGGAGATCGCCGAG
GCGGTGCACCGCCTGTGGCGGCTGGTGTCGTGGGCCAGCCTGCAGCGCCGGCTGCAGGAGGCGATGCGCCTGCCGCGGCG
CGGTCCGCCCGCGGGCGGGCTGCGCGCGGCGTTTCTGGTGCGCGACAACCTGCGTCACCGGCGCGACATCGAGGACGCCT
ACCTTGCCGCGATCGGGCGCGCGCGCGAGGAGATCGTGATCGCCAACGCGTACTTCTTCCCCGGGCGGCAGTTCCGCCAG
GCCCTGCTGGAGGCCGCCGCGCGCGGGGTGCGGGTCACGCTGGTGCTGCAGGGGCTGGCCGACCACCCGGTGCTCGCTCA
CGCCACGCGTGCGCTCTACCCGCACTTCCTGCAGCGCGGCATCCGCCTTTTCGAGTACCACCGCAGCTATCTGCACGCCA
AGGTGGCCGTGGTGGACCGGCGCTGGGCCACGGTGGGGTCGAGCAACATCGACCCGTTCAGCCTGTGGCTGGCGCGCGAG
GCCAACGTGGTGGTGGAAGATGCCGGCTTCGCCGGATCGCTGCACCGCAGTCTGGAGTCCGCAGTGCAGGAGGGCGCGCG
CGAACTGCATCGCGAAGACTGGCGGCGCCAGCGGACCTTGCGGCGGGTTGCGAGCTGGCTGGCCTACCAGGCCGTACGCC
TCGCGATCGGCATCGCGGGCTACGGCGGGCGGCACTGA

Upstream 100 bases:

>100_bases
TGCGCGGTTTCCAGGTCCGCCGCGCCGAAGTCCATTACGGCGAGCCCTGGTCGCGCATCTCGGATCACGCCGCGCTCACC
GCCGACCTGGAGCTTGTGCA

Downstream 100 bases:

>100_bases
GCGGGATCAGGCGCCCGGTCGCGGCAGGCTGCGTCCGGCGCGCTTGGCCGAAATTACGCCGGTATTGACGCCCACCCAGT
TGAGCCCGCCAAAGAGGCGG

Product: putative cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MTVLVEGNAIALLENGLQYFPALEAEIDSARHEIFLETYIFSRDVVGRRIAAALVRAAARGVRVRVLVDGFGGREFVRDL
MPGLLADGVEVLIFRRELRLTSLRRHRLRRMHRKIALIDARVAFVGGINITDDFELSGPPHPRYDYAVRVEGPLLAEIAE
AVHRLWRLVSWASLQRRLQEAMRLPRRGPPAGGLRAAFLVRDNLRHRRDIEDAYLAAIGRAREEIVIANAYFFPGRQFRQ
ALLEAAARGVRVTLVLQGLADHPVLAHATRALYPHFLQRGIRLFEYHRSYLHAKVAVVDRRWATVGSSNIDPFSLWLARE
ANVVVEDAGFAGSLHRSLESAVQEGARELHREDWRRQRTLRRVASWLAYQAVRLAIGIAGYGGRH

Sequences:

>Translated_385_residues
MTVLVEGNAIALLENGLQYFPALEAEIDSARHEIFLETYIFSRDVVGRRIAAALVRAAARGVRVRVLVDGFGGREFVRDL
MPGLLADGVEVLIFRRELRLTSLRRHRLRRMHRKIALIDARVAFVGGINITDDFELSGPPHPRYDYAVRVEGPLLAEIAE
AVHRLWRLVSWASLQRRLQEAMRLPRRGPPAGGLRAAFLVRDNLRHRRDIEDAYLAAIGRAREEIVIANAYFFPGRQFRQ
ALLEAAARGVRVTLVLQGLADHPVLAHATRALYPHFLQRGIRLFEYHRSYLHAKVAVVDRRWATVGSSNIDPFSLWLARE
ANVVVEDAGFAGSLHRSLESAVQEGARELHREDWRRQRTLRRVASWLAYQAVRLAIGIAGYGGRH
>Mature_384_residues
TVLVEGNAIALLENGLQYFPALEAEIDSARHEIFLETYIFSRDVVGRRIAAALVRAAARGVRVRVLVDGFGGREFVRDLM
PGLLADGVEVLIFRRELRLTSLRRHRLRRMHRKIALIDARVAFVGGINITDDFELSGPPHPRYDYAVRVEGPLLAEIAEA
VHRLWRLVSWASLQRRLQEAMRLPRRGPPAGGLRAAFLVRDNLRHRRDIEDAYLAAIGRAREEIVIANAYFFPGRQFRQA
LLEAAARGVRVTLVLQGLADHPVLAHATRALYPHFLQRGIRLFEYHRSYLHAKVAVVDRRWATVGSSNIDPFSLWLAREA
NVVVEDAGFAGSLHRSLESAVQEGARELHREDWRRQRTLRRVASWLAYQAVRLAIGIAGYGGRH

Specific function: Catalyzes, in vitro, the phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787007, Length=390, Percent_Identity=40, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1787502, Length=373, Percent_Identity=28.686327077748, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI87081826, Length=423, Percent_Identity=27.1867612293144, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 43634; Mature: 43503

Theoretical pI: Translated: 11.38; Mature: 11.38

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLVEGNAIALLENGLQYFPALEAEIDSARHEIFLETYIFSRDVVGRRIAAALVRAAAR
CEEEEECCEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVRVRVLVDGFGGREFVRDLMPGLLADGVEVLIFRRELRLTSLRRHRLRRMHRKIALIDA
CCEEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVAFVGGINITDDFELSGPPHPRYDYAVRVEGPLLAEIAEAVHRLWRLVSWASLQRRLQE
HHHEEECCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMRLPRRGPPAGGLRAAFLVRDNLRHRRDIEDAYLAAIGRAREEIVIANAYFFPGRQFRQ
HHCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCEEEEEEEEECCCHHHHH
ALLEAAARGVRVTLVLQGLADHPVLAHATRALYPHFLQRGIRLFEYHRSYLHAKVAVVDR
HHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RWATVGSSNIDPFSLWLAREANVVVEDAGFAGSLHRSLESAVQEGARELHREDWRRQRTL
HHHCCCCCCCCHHHHEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRVASWLAYQAVRLAIGIAGYGGRH
HHHHHHHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure 
TVLVEGNAIALLENGLQYFPALEAEIDSARHEIFLETYIFSRDVVGRRIAAALVRAAAR
EEEEECCEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVRVRVLVDGFGGREFVRDLMPGLLADGVEVLIFRRELRLTSLRRHRLRRMHRKIALIDA
CCEEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVAFVGGINITDDFELSGPPHPRYDYAVRVEGPLLAEIAEAVHRLWRLVSWASLQRRLQE
HHHEEECCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMRLPRRGPPAGGLRAAFLVRDNLRHRRDIEDAYLAAIGRAREEIVIANAYFFPGRQFRQ
HHCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCEEEEEEEEECCCHHHHH
ALLEAAARGVRVTLVLQGLADHPVLAHATRALYPHFLQRGIRLFEYHRSYLHAKVAVVDR
HHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RWATVGSSNIDPFSLWLAREANVVVEDAGFAGSLHRSLESAVQEGARELHREDWRRQRTL
HHHCCCCCCCCHHHHEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRVASWLAYQAVRLAIGIAGYGGRH
HHHHHHHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]