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The map label for this gene is ntrB [H]

Identifier: 119897027

GI number: 119897027

Start: 786724

End: 787797

Strand: Reverse

Name: ntrB [H]

Synonym: azo0736

Alternate gene names: 119897027

Gene position: 787797-786724 (Counterclockwise)

Preceding gene: 119897028

Following gene: 119897026

Centisome position: 18.0

GC content: 65.36

Gene sequence:

>1074_bases
ATGGCTCGATCCTCCTCCTCCCAGCCGATCGCGGCAGAGAGTTTCAATGGCCTCGACCTGCTGTCTTCCGCGGTCGTGAT
GATCGATGAAAGACGCGTCATCCGTTACATCAATCCGGGCGCAGAAAACCTGTTCGCGATCAGCCAGCGCAAGCTGCTCG
GCGCACCGCTCGCGCGCCTGCTCGGCGAACCCGCCGGGCTCGGCACCGCGCTCGACAACGTGCTCCGCACCAACTGGAGC
TACACCGGGCAGGACCTGACCATCGCGCGCCCCGCGTCGGAAGCCATCCATGTCGATTGCACGGTCAGCCCGGTCGACAT
CGCGGGCGTCAAGCTGCTGCTCGAGTTCCGCCCGATCGACGCCCAGTTGCGCGTCGCCCGCGAGGAGCAACTGCTGCAGC
AGCAGCAGGCCAACCGCGAGCTGATCCGCAACCTCGCGCACGAGATCAAGAATCCGCTCGGCGGCATCCGCGGCTCGGCA
CAGCTGCTGGAGCGCGAACTCGCCGATCCGCAACTGCGCGAATACACCGAGGTCATCATCGCTGAAGCCGACCGGCTGCA
GGACCTGATGAACCGGCTGCTGACCTCGCACTCGATGATGCGGCCCGCGCAGCTCAACATCCATGATGTCCTGGAGCGCG
TGCGCCGCCTGATCCTCGCCGAGTTCCCGGACGTGGTGATCCGGCGCGACTACGACACCAGCCTGCCCGAACTCACCGCC
GACCGCGAACAACTGATCCAGGCCATCCTCAACATCGCGCGCAACGCGGCGCAGGCCCTGGGCGGAAAGGGCGAGATCCG
GCTGCGCACCCGGGTCGCACGCCAGGTCACGCTCGCCAAGCGCCGCTACAAGCTGGCACTGGAATTGCAAGTGATCGACA
ACGGCCCCGGTATTCCGGATGAGATTCGCGACAAGATCTTCTACCCGCTTGTTTCGGGCCGCGACGGTGGTAGCGGACTG
GGCTTGTCACTGGCCCAGAGCTTCGTGGAGCAGCACCAGGGCATGATCGACGTCGATAGCCGCCCGGGACGAACCTGCTT
TTGCATCCTGCTGCCGATTACCGATCGGACCTGA

Upstream 100 bases:

>100_bases
ATCGAAGCACTGAAGCGCGAGATCGCCAACCTGCGCTGACGCGAAGCCGGGGAGCACGCGCCCCGGTCCGACTCACCTCC
GTTCCGGTCGCCCGTTCCCC

Downstream 100 bases:

>100_bases
CGCATCCATCGCGTCGTCCGCACCAACTTAGTGCTCTATTACGATGAATACCGTCTGGATCGTGGATGACGACCGCTCCA
TCCGCTGGGTGCTGGAAAAG

Product: nitrogen regulation protein NR(II)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MARSSSSQPIAAESFNGLDLLSSAVVMIDERRVIRYINPGAENLFAISQRKLLGAPLARLLGEPAGLGTALDNVLRTNWS
YTGQDLTIARPASEAIHVDCTVSPVDIAGVKLLLEFRPIDAQLRVAREEQLLQQQQANRELIRNLAHEIKNPLGGIRGSA
QLLERELADPQLREYTEVIIAEADRLQDLMNRLLTSHSMMRPAQLNIHDVLERVRRLILAEFPDVVIRRDYDTSLPELTA
DREQLIQAILNIARNAAQALGGKGEIRLRTRVARQVTLAKRRYKLALELQVIDNGPGIPDEIRDKIFYPLVSGRDGGSGL
GLSLAQSFVEQHQGMIDVDSRPGRTCFCILLPITDRT

Sequences:

>Translated_357_residues
MARSSSSQPIAAESFNGLDLLSSAVVMIDERRVIRYINPGAENLFAISQRKLLGAPLARLLGEPAGLGTALDNVLRTNWS
YTGQDLTIARPASEAIHVDCTVSPVDIAGVKLLLEFRPIDAQLRVAREEQLLQQQQANRELIRNLAHEIKNPLGGIRGSA
QLLERELADPQLREYTEVIIAEADRLQDLMNRLLTSHSMMRPAQLNIHDVLERVRRLILAEFPDVVIRRDYDTSLPELTA
DREQLIQAILNIARNAAQALGGKGEIRLRTRVARQVTLAKRRYKLALELQVIDNGPGIPDEIRDKIFYPLVSGRDGGSGL
GLSLAQSFVEQHQGMIDVDSRPGRTCFCILLPITDRT
>Mature_356_residues
ARSSSSQPIAAESFNGLDLLSSAVVMIDERRVIRYINPGAENLFAISQRKLLGAPLARLLGEPAGLGTALDNVLRTNWSY
TGQDLTIARPASEAIHVDCTVSPVDIAGVKLLLEFRPIDAQLRVAREEQLLQQQQANRELIRNLAHEIKNPLGGIRGSAQ
LLERELADPQLREYTEVIIAEADRLQDLMNRLLTSHSMMRPAQLNIHDVLERVRRLILAEFPDVVIRRDYDTSLPELTAD
REQLIQAILNIARNAAQALGGKGEIRLRTRVARQVTLAKRRYKLALELQVIDNGPGIPDEIRDKIFYPLVSGRDGGSGLG
LSLAQSFVEQHQGMIDVDSRPGRTCFCILLPITDRT

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=352, Percent_Identity=45.7386363636364, Blast_Score=281, Evalue=6e-77,
Organism=Escherichia coli, GI1788549, Length=354, Percent_Identity=30.225988700565, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1790436, Length=245, Percent_Identity=32.2448979591837, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1786912, Length=250, Percent_Identity=26.4, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082128, Length=244, Percent_Identity=27.0491803278689, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1786600, Length=246, Percent_Identity=26.4227642276423, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789149, Length=235, Percent_Identity=28.0851063829787, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790346, Length=254, Percent_Identity=27.5590551181102, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87081816, Length=233, Percent_Identity=28.3261802575107, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 39694; Mature: 39563

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSSSSQPIAAESFNGLDLLSSAVVMIDERRVIRYINPGAENLFAISQRKLLGAPLARL
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
LGEPAGLGTALDNVLRTNWSYTGQDLTIARPASEAIHVDCTVSPVDIAGVKLLLEFRPID
HCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEEEECCCCHHHCEEEEEEECCCC
AQLRVAREEQLLQQQQANRELIRNLAHEIKNPLGGIRGSAQLLERELADPQLREYTEVII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHH
AEADRLQDLMNRLLTSHSMMRPAQLNIHDVLERVRRLILAEFPDVVIRRDYDTSLPELTA
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHEEECCCCCCCCCCCC
DREQLIQAILNIARNAAQALGGKGEIRLRTRVARQVTLAKRRYKLALELQVIDNGPGIPD
CHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCH
EIRDKIFYPLVSGRDGGSGLGLSLAQSFVEQHQGMIDVDSRPGRTCFCILLPITDRT
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCEEEEEEEECCCCC
>Mature Secondary Structure 
ARSSSSQPIAAESFNGLDLLSSAVVMIDERRVIRYINPGAENLFAISQRKLLGAPLARL
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
LGEPAGLGTALDNVLRTNWSYTGQDLTIARPASEAIHVDCTVSPVDIAGVKLLLEFRPID
HCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEEEECCCCHHHCEEEEEEECCCC
AQLRVAREEQLLQQQQANRELIRNLAHEIKNPLGGIRGSAQLLERELADPQLREYTEVII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHH
AEADRLQDLMNRLLTSHSMMRPAQLNIHDVLERVRRLILAEFPDVVIRRDYDTSLPELTA
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHEEECCCCCCCCCCCC
DREQLIQAILNIARNAAQALGGKGEIRLRTRVARQVTLAKRRYKLALELQVIDNGPGIPD
CHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCH
EIRDKIFYPLVSGRDGGSGLGLSLAQSFVEQHQGMIDVDSRPGRTCFCILLPITDRT
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]