Click here to switch to the map view.

The map label for this gene is ntrC [H]

Identifier: 119897026

GI number: 119897026

Start: 785250

End: 786680

Strand: Reverse

Name: ntrC [H]

Synonym: azo0735

Alternate gene names: 119897026

Gene position: 786680-785250 (Counterclockwise)

Preceding gene: 119897027

Following gene: 119897025

Centisome position: 17.98

GC content: 67.65

Gene sequence:

>1431_bases
ATGAATACCGTCTGGATCGTGGATGACGACCGCTCCATCCGCTGGGTGCTGGAAAAGGCCCTCAGCCGAGAGGAGATTCC
CCACCGCAGCTTCACCTCGGCCAACGAGGCGCTGGCCGAACTCGAATCGACCGCCCATCCGCCCAAGGTGCTGGTTTCCG
ACATCCGGATGCCCGGCGAAAGCGGGCTGACCCTGCTGCAGCGGGTCAAGAATCTGCACCCCCACCTTCCGGTCATCATC
ATGACCGCGTACTCCGACCTCGAGAGCGCGGTCGCCGCCTTCCAGGGCGGCGCGTTTGAATACCTGCCGAAGCCCTTCGA
TGTGGACCAGGCAGTGGCCCTGGTGCGGCGTGCAATCGACCAGAGCGCGCACCAGGAAGGTGCGCAAGAGGAAACCGCGC
TCGCGCCCGAGATTCTCGGCCAGGCGCCGTCGATGCAGGAGGTGTTCCGCGCCATTGGCCGCCTGTCGCAGTCGCACGCG
ACCGTGCTGATCAATGGTGAATCCGGCACCGGCAAGGAACTGGTGGCACGCGCCCTGCACCGGCATAGCCCGCGACGCGA
CGCGCCCTTCATCGCGATCAACACCGCCGCGATCCCGCGCGACCTGCTCGAATCCGAGTTGTTCGGCCATGAGCGCGGCG
CCTTCACCGGCGCTGCCGCACAGCGCCGCGGCCGCTTCGAACAGGCCGAGAACGGCACGCTGTTCCTCGACGAAATCGGC
GACATGCCGTCCGAACTGCAGACCCGGCTGCTGCGCGTGCTGTCCGATGGCAACTTCTACCGGGTGGGCGGCCACCAGCC
GATCAAGGCCAATGTGCGGGTGATCGCCGCCACCCACCAGGATCTCGAAGAGCGCGTCCGCCTCGGGCTGTTCCGCGAGG
ACCTCTTCCACCGCCTCAACGTGATCCGGCTGCGCCTGCCGCCGCTGCGCGAACGCCGCGAGGACGTGCCCATCCTGGTC
CGCCACTTCCTGCAACGCAGCGCGCAGGAACTCGGTGTGGAGAGCAAGCGCATCTCGGAAGGCGCCATCAAGTACCTGCA
GGCGCTGCCCTTCCCCGGCAACGTGCGTCAGCTCGAGAACCTGTGCCACTGGCTCACGGTGATGGCGCCGGGGCAGGTGG
TCGAAGTGGCCGACCTGCCGCCCGAAATGCGCGACCAGCCGACCCGCGAGGCCCCGGTGAACTGGGTGGACGGTCTCGGT
CTCGAAGCAGATCGCATGATCGCCGCCAACCCCGGCGAGGTCTTCGACAAGCTCACGCGCGAATTCGAGCGCACGCTGAT
CCGCCGCGCGCTGACGGCCACGGGCGGACGGCGCATCGAGGCCGCCCAGTTGCTCGGGATCGGGCGCAACACCATCACGC
GCAAGATCCAGGAACTGGGCATGGACGACGATCACCGGCCAGCGGCCGGCGAAGGCGAGCACGAGGACTGA

Upstream 100 bases:

>100_bases
AGCCGCCCGGGACGAACCTGCTTTTGCATCCTGCTGCCGATTACCGATCGGACCTGACGCATCCATCGCGTCGTCCGCAC
CAACTTAGTGCTCTATTACG

Downstream 100 bases:

>100_bases
CGCAGCGGCGTCGGCGGCGATGCACCACGCTCGTGCATCGCCCCTCAATGACGGATAATGCGCAGCCTTTACGGCCATTG
TCCTGACTGTCGATGAGCGC

Product: nitrogen regulation protein NR(I)

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MNTVWIVDDDRSIRWVLEKALSREEIPHRSFTSANEALAELESTAHPPKVLVSDIRMPGESGLTLLQRVKNLHPHLPVII
MTAYSDLESAVAAFQGGAFEYLPKPFDVDQAVALVRRAIDQSAHQEGAQEETALAPEILGQAPSMQEVFRAIGRLSQSHA
TVLINGESGTGKELVARALHRHSPRRDAPFIAINTAAIPRDLLESELFGHERGAFTGAAAQRRGRFEQAENGTLFLDEIG
DMPSELQTRLLRVLSDGNFYRVGGHQPIKANVRVIAATHQDLEERVRLGLFREDLFHRLNVIRLRLPPLRERREDVPILV
RHFLQRSAQELGVESKRISEGAIKYLQALPFPGNVRQLENLCHWLTVMAPGQVVEVADLPPEMRDQPTREAPVNWVDGLG
LEADRMIAANPGEVFDKLTREFERTLIRRALTATGGRRIEAAQLLGIGRNTITRKIQELGMDDDHRPAAGEGEHED

Sequences:

>Translated_476_residues
MNTVWIVDDDRSIRWVLEKALSREEIPHRSFTSANEALAELESTAHPPKVLVSDIRMPGESGLTLLQRVKNLHPHLPVII
MTAYSDLESAVAAFQGGAFEYLPKPFDVDQAVALVRRAIDQSAHQEGAQEETALAPEILGQAPSMQEVFRAIGRLSQSHA
TVLINGESGTGKELVARALHRHSPRRDAPFIAINTAAIPRDLLESELFGHERGAFTGAAAQRRGRFEQAENGTLFLDEIG
DMPSELQTRLLRVLSDGNFYRVGGHQPIKANVRVIAATHQDLEERVRLGLFREDLFHRLNVIRLRLPPLRERREDVPILV
RHFLQRSAQELGVESKRISEGAIKYLQALPFPGNVRQLENLCHWLTVMAPGQVVEVADLPPEMRDQPTREAPVNWVDGLG
LEADRMIAANPGEVFDKLTREFERTLIRRALTATGGRRIEAAQLLGIGRNTITRKIQELGMDDDHRPAAGEGEHED
>Mature_476_residues
MNTVWIVDDDRSIRWVLEKALSREEIPHRSFTSANEALAELESTAHPPKVLVSDIRMPGESGLTLLQRVKNLHPHLPVII
MTAYSDLESAVAAFQGGAFEYLPKPFDVDQAVALVRRAIDQSAHQEGAQEETALAPEILGQAPSMQEVFRAIGRLSQSHA
TVLINGESGTGKELVARALHRHSPRRDAPFIAINTAAIPRDLLESELFGHERGAFTGAAAQRRGRFEQAENGTLFLDEIG
DMPSELQTRLLRVLSDGNFYRVGGHQPIKANVRVIAATHQDLEERVRLGLFREDLFHRLNVIRLRLPPLRERREDVPILV
RHFLQRSAQELGVESKRISEGAIKYLQALPFPGNVRQLENLCHWLTVMAPGQVVEVADLPPEMRDQPTREAPVNWVDGLG
LEADRMIAANPGEVFDKLTREFERTLIRRALTATGGRRIEAAQLLGIGRNTITRKIQELGMDDDHRPAAGEGEHED

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=466, Percent_Identity=60.5150214592275, Blast_Score=547, Evalue=1e-157,
Organism=Escherichia coli, GI1788550, Length=469, Percent_Identity=42.8571428571429, Blast_Score=354, Evalue=7e-99,
Organism=Escherichia coli, GI1790437, Length=455, Percent_Identity=40.4395604395604, Blast_Score=312, Evalue=2e-86,
Organism=Escherichia coli, GI1788905, Length=413, Percent_Identity=38.9830508474576, Blast_Score=281, Evalue=4e-77,
Organism=Escherichia coli, GI87082117, Length=362, Percent_Identity=41.4364640883978, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1789233, Length=344, Percent_Identity=42.1511627906977, Blast_Score=249, Evalue=4e-67,
Organism=Escherichia coli, GI1789087, Length=329, Percent_Identity=43.4650455927052, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI87082152, Length=233, Percent_Identity=49.3562231759657, Blast_Score=225, Evalue=5e-60,
Organism=Escherichia coli, GI1786524, Length=338, Percent_Identity=42.3076923076923, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1787583, Length=352, Percent_Identity=35.7954545454545, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI87081872, Length=229, Percent_Identity=44.9781659388646, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI87081858, Length=463, Percent_Identity=28.2937365010799, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789828, Length=344, Percent_Identity=29.3604651162791, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789809, Length=176, Percent_Identity=27.2727272727273, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 53159; Mature: 53159

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTVWIVDDDRSIRWVLEKALSREEIPHRSFTSANEALAELESTAHPPKVLVSDIRMPGE
CCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCC
SGLTLLQRVKNLHPHLPVIIMTAYSDLESAVAAFQGGAFEYLPKPFDVDQAVALVRRAID
CHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHH
QSAHQEGAQEETALAPEILGQAPSMQEVFRAIGRLSQSHATVLINGESGTGKELVARALH
HHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHH
RHSPRRDAPFIAINTAAIPRDLLESELFGHERGAFTGAAAQRRGRFEQAENGTLFLDEIG
HCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHCCCCHHCCCCCEEHHHHC
DMPSELQTRLLRVLSDGNFYRVGGHQPIKANVRVIAATHQDLEERVRLGLFREDLFHRLN
CCHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
VIRLRLPPLRERREDVPILVRHFLQRSAQELGVESKRISEGAIKYLQALPFPGNVRQLEN
EEEECCCCHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
LCHWLTVMAPGQVVEVADLPPEMRDQPTREAPVNWVDGLGLEADRMIAANPGEVFDKLTR
HHHHHHHCCCCCEEEECCCCHHHCCCCCCCCCCCHHHCCCCCCCCEEECCCHHHHHHHHH
EFERTLIRRALTATGGRRIEAAQLLGIGRNTITRKIQELGMDDDHRPAAGEGEHED
HHHHHHHHHHHHHCCCCEEHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNTVWIVDDDRSIRWVLEKALSREEIPHRSFTSANEALAELESTAHPPKVLVSDIRMPGE
CCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCC
SGLTLLQRVKNLHPHLPVIIMTAYSDLESAVAAFQGGAFEYLPKPFDVDQAVALVRRAID
CHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHH
QSAHQEGAQEETALAPEILGQAPSMQEVFRAIGRLSQSHATVLINGESGTGKELVARALH
HHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHH
RHSPRRDAPFIAINTAAIPRDLLESELFGHERGAFTGAAAQRRGRFEQAENGTLFLDEIG
HCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHCCCCHHCCCCCEEHHHHC
DMPSELQTRLLRVLSDGNFYRVGGHQPIKANVRVIAATHQDLEERVRLGLFREDLFHRLN
CCHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
VIRLRLPPLRERREDVPILVRHFLQRSAQELGVESKRISEGAIKYLQALPFPGNVRQLEN
EEEECCCCHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
LCHWLTVMAPGQVVEVADLPPEMRDQPTREAPVNWVDGLGLEADRMIAANPGEVFDKLTR
HHHHHHHCCCCCEEEECCCCHHHCCCCCCCCCCCHHHCCCCCCCCEEECCCHHHHHHHHH
EFERTLIRRALTATGGRRIEAAQLLGIGRNTITRKIQELGMDDDHRPAAGEGEHED
HHHHHHHHHHHHHCCCCEEHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]