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The map label for this gene is metK

Identifier: 119896880

GI number: 119896880

Start: 620087

End: 621253

Strand: Reverse

Name: metK

Synonym: azo0589

Alternate gene names: 119896880

Gene position: 621253-620087 (Counterclockwise)

Preceding gene: 119896884

Following gene: 119896878

Centisome position: 14.2

GC content: 66.75

Gene sequence:

>1167_bases
ATGGCCAACGAGTTTCTCTTCACCTCCGAATCGGTCTCCGAAGGCCACCCGGACAAGGTTGCCGACCAGATCTCCGATGG
CGTGCTCGACGCCATCCTGGCCGAAGACCCCAAGGCGCGCGTCGCCTGCGAAACCCTGGTGTCCACCGGCCTGGTGGTGA
TCTCCGGCGAAATCACCACCTCCGCCCACCCCAACTACCGCGAGATCGCGCAGGATGTGGTGCGCCGCATCGGCTACGAC
AACTCCGACATCGGCTTCGACTACAAGTCGTGCGCCGTGCTGGCGGCGATCAATCGCCAGTCGTCCGACATCGCCCAGGG
CGTGAATGAAGGCGAAGGGCTCGATCTCGACCAGGGTGCCGGCGACCAGGGCCTGATGTTCGGCTACGCCACCAACGAGA
CCCCCAGCCTGATGCCGCTGCCGATCTACTACGCGCACCGCATCATGCAGCGCCAGGCCGAAGTGCGTAAGGACGGCCGC
CTGCCCTGGCTGCGCCCGGACGCCAAGAGCCAGCTCACGGTGAAGTATGTCGACGGCAAGCCGGTGGCGATCGACACCGT
GGTCGTGTCGACGCAGCACAACCCGGAGGTGTCGCACGCGCAGATTTCCGAGGCGGTGATCGAGGAGATCATCAAGCCGG
TGCTGCCCAAGGAGCTGATGCAGGGCGAGGTGCGCTACCTGATCAACCCCACCGGCCGCTTCGTCATCGGCGGCCCGCAC
GGCGACTGCGGCCTCACCGGGCGCAAGATCATCGTCGACACCTACGGCGGCGCAGCCCACCACGGCGGCGGTGCCTTCTC
CGGCAAGGATCCGTCCAAGGTCGACCGTTCCGCCGCCTACGCCGGCCGCTACGTCGCCAAGAACGTGGTCGCCGCCGGTC
TGGCCGACAAGTGCGAAGTGCAGGTCGCCTACGCGATCGGCGTCGCCAAGCCGGTGTCGCTGATGGTGAATACCTTCGGC
ACCGGCAAGATCGCCGACGACAAGATCGTCGACCTGATCCGTGCCCACTTCGACCTCCGCCCCAAGGCCATCATCCAGAC
CCTGGACCTGCTGCGCCCGATCTATTCCCGCACCGCCGCCTACGGCCACTTCGGCCGTGACGAGCCGGAGTTCACGTGGG
AGCAGACCGACAAGGCCTCCGCGCTGCGCGCCGCCGCCGGTCTCTAA

Upstream 100 bases:

>100_bases
CACCAGAAATACTGCTAAAGCGTCGGCTGCTCGCCGAAATCCCCGGAGCTGGCCACGACGCCACAGAACCGGGGATTTTT
GTTTTTCAGGAGCAGAACGC

Downstream 100 bases:

>100_bases
GCACGGTACTGCCGCGGGCGTGCGCGCCCGCGGCACGGCTTACTGGAATGTGGACAGACGTTCGCCCGCGCCCTGCAGCG
GGCCCAGCGGGTTGCGGCTG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MANEFLFTSESVSEGHPDKVADQISDGVLDAILAEDPKARVACETLVSTGLVVISGEITTSAHPNYREIAQDVVRRIGYD
NSDIGFDYKSCAVLAAINRQSSDIAQGVNEGEGLDLDQGAGDQGLMFGYATNETPSLMPLPIYYAHRIMQRQAEVRKDGR
LPWLRPDAKSQLTVKYVDGKPVAIDTVVVSTQHNPEVSHAQISEAVIEEIIKPVLPKELMQGEVRYLINPTGRFVIGGPH
GDCGLTGRKIIVDTYGGAAHHGGGAFSGKDPSKVDRSAAYAGRYVAKNVVAAGLADKCEVQVAYAIGVAKPVSLMVNTFG
TGKIADDKIVDLIRAHFDLRPKAIIQTLDLLRPIYSRTAAYGHFGRDEPEFTWEQTDKASALRAAAGL

Sequences:

>Translated_388_residues
MANEFLFTSESVSEGHPDKVADQISDGVLDAILAEDPKARVACETLVSTGLVVISGEITTSAHPNYREIAQDVVRRIGYD
NSDIGFDYKSCAVLAAINRQSSDIAQGVNEGEGLDLDQGAGDQGLMFGYATNETPSLMPLPIYYAHRIMQRQAEVRKDGR
LPWLRPDAKSQLTVKYVDGKPVAIDTVVVSTQHNPEVSHAQISEAVIEEIIKPVLPKELMQGEVRYLINPTGRFVIGGPH
GDCGLTGRKIIVDTYGGAAHHGGGAFSGKDPSKVDRSAAYAGRYVAKNVVAAGLADKCEVQVAYAIGVAKPVSLMVNTFG
TGKIADDKIVDLIRAHFDLRPKAIIQTLDLLRPIYSRTAAYGHFGRDEPEFTWEQTDKASALRAAAGL
>Mature_387_residues
ANEFLFTSESVSEGHPDKVADQISDGVLDAILAEDPKARVACETLVSTGLVVISGEITTSAHPNYREIAQDVVRRIGYDN
SDIGFDYKSCAVLAAINRQSSDIAQGVNEGEGLDLDQGAGDQGLMFGYATNETPSLMPLPIYYAHRIMQRQAEVRKDGRL
PWLRPDAKSQLTVKYVDGKPVAIDTVVVSTQHNPEVSHAQISEAVIEEIIKPVLPKELMQGEVRYLINPTGRFVIGGPHG
DCGLTGRKIIVDTYGGAAHHGGGAFSGKDPSKVDRSAAYAGRYVAKNVVAAGLADKCEVQVAYAIGVAKPVSLMVNTFGT
GKIADDKIVDLIRAHFDLRPKAIIQTLDLLRPIYSRTAAYGHFGRDEPEFTWEQTDKASALRAAAGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=374, Percent_Identity=54.8128342245989, Blast_Score=413, Evalue=1e-115,
Organism=Homo sapiens, GI4557737, Length=374, Percent_Identity=54.5454545454545, Blast_Score=411, Evalue=1e-115,
Organism=Escherichia coli, GI1789311, Length=383, Percent_Identity=69.9738903394256, Blast_Score=553, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI17538498, Length=377, Percent_Identity=54.9071618037135, Blast_Score=417, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17538494, Length=377, Percent_Identity=54.6419098143236, Blast_Score=415, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI25145633, Length=380, Percent_Identity=54.4736842105263, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17551082, Length=382, Percent_Identity=53.4031413612565, Blast_Score=406, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI32565905, Length=377, Percent_Identity=50.132625994695, Blast_Score=354, Evalue=6e-98,
Organism=Caenorhabditis elegans, GI32565911, Length=329, Percent_Identity=51.9756838905775, Blast_Score=342, Evalue=2e-94,
Organism=Caenorhabditis elegans, GI25145635, Length=332, Percent_Identity=51.2048192771084, Blast_Score=337, Evalue=8e-93,
Organism=Caenorhabditis elegans, GI32565903, Length=302, Percent_Identity=52.6490066225166, Blast_Score=308, Evalue=3e-84,
Organism=Caenorhabditis elegans, GI32565909, Length=84, Percent_Identity=58.3333333333333, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI32565907, Length=41, Percent_Identity=78.0487804878049, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6323209, Length=379, Percent_Identity=56.9920844327177, Blast_Score=432, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6320710, Length=381, Percent_Identity=55.6430446194226, Blast_Score=431, Evalue=1e-122,
Organism=Drosophila melanogaster, GI45552159, Length=375, Percent_Identity=51.7333333333333, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580529, Length=375, Percent_Identity=51.7333333333333, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580537, Length=375, Percent_Identity=51.7333333333333, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580533, Length=375, Percent_Identity=51.7333333333333, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580535, Length=375, Percent_Identity=51.7333333333333, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580531, Length=375, Percent_Identity=51.7333333333333, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580527, Length=375, Percent_Identity=51.7333333333333, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580525, Length=375, Percent_Identity=51.7333333333333, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580541, Length=376, Percent_Identity=49.2021276595745, Blast_Score=347, Evalue=7e-96,
Organism=Drosophila melanogaster, GI24580539, Length=119, Percent_Identity=53.781512605042, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_AZOSB (A1K301)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932093.1
- ProteinModelPortal:   A1K301
- SMR:   A1K301
- STRING:   A1K301
- GeneID:   4606463
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo0589
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- PhylomeDB:   A1K301
- ProtClustDB:   PRK05250
- BioCyc:   ASP62928:AZO0589-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 41819; Mature: 41688

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANEFLFTSESVSEGHPDKVADQISDGVLDAILAEDPKARVACETLVSTGLVVISGEITT
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEEECCEEC
SAHPNYREIAQDVVRRIGYDNSDIGFDYKSCAVLAAINRQSSDIAQGVNEGEGLDLDQGA
CCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCC
GDQGLMFGYATNETPSLMPLPIYYAHRIMQRQAEVRKDGRLPWLRPDAKSQLTVKYVDGK
CCCCEEEEEECCCCCCCEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCC
PVAIDTVVVSTQHNPEVSHAQISEAVIEEIIKPVLPKELMQGEVRYLINPTGRFVIGGPH
CEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEECCCCCEEEECCC
GDCGLTGRKIIVDTYGGAAHHGGGAFSGKDPSKVDRSAAYAGRYVAKNVVAAGLADKCEV
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
QVAYAIGVAKPVSLMVNTFGTGKIADDKIVDLIRAHFDLRPKAIIQTLDLLRPIYSRTAA
EEEEECCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
YGHFGRDEPEFTWEQTDKASALRAAAGL
HCCCCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
ANEFLFTSESVSEGHPDKVADQISDGVLDAILAEDPKARVACETLVSTGLVVISGEITT
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEEECCEEC
SAHPNYREIAQDVVRRIGYDNSDIGFDYKSCAVLAAINRQSSDIAQGVNEGEGLDLDQGA
CCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCC
GDQGLMFGYATNETPSLMPLPIYYAHRIMQRQAEVRKDGRLPWLRPDAKSQLTVKYVDGK
CCCCEEEEEECCCCCCCEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCC
PVAIDTVVVSTQHNPEVSHAQISEAVIEEIIKPVLPKELMQGEVRYLINPTGRFVIGGPH
CEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEECCCCCEEEECCC
GDCGLTGRKIIVDTYGGAAHHGGGAFSGKDPSKVDRSAAYAGRYVAKNVVAAGLADKCEV
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
QVAYAIGVAKPVSLMVNTFGTGKIADDKIVDLIRAHFDLRPKAIIQTLDLLRPIYSRTAA
EEEEECCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
YGHFGRDEPEFTWEQTDKASALRAAAGL
HCCCCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA