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The map label for this gene is phaZ1 [H]

Identifier: 119896861

GI number: 119896861

Start: 600348

End: 601409

Strand: Reverse

Name: phaZ1 [H]

Synonym: azo0570

Alternate gene names: 119896861

Gene position: 601409-600348 (Counterclockwise)

Preceding gene: 119896862

Following gene: 119896860

Centisome position: 13.74

GC content: 74.39

Gene sequence:

>1062_bases
ATGAGCCGACGCACGCGCAAGTCTCCCCTCGGCACTGCCTTCCAGCGCACGCTGCGCTCGCTCACGCGCGCAAGCGTACG
CGCAGGCACCAAGGCGCTCGATCAGGCCGCGCGTATCGCGGCGGAGCACGCCCGGCCGCCGCCTGGCCAGGGCGACTGGA
TCGCCGGCCAGGCACTCGGGCCCGCGGGCGTGCGCCGCTACTACCTGTTCCGTCCCGACGGCATCACGCGCACTCAGCGC
GTGCCGCTGATGGTGATGCTGCACGGCTGCGGTCAGACGGCGCGCAGCTTTGCGCTGAGCACGCGCATGAACGCGCTCGC
CGCGCGCGAACGTTTCCTGGTGCTGTACCCGGAGCAGGACCGACGGGCGAATCCGCAGGGCTGCTGGGAATGGTTCGGTA
CCGCCACCCGCACCGGCCACGCCGAGGCCGCCACGCTGCTGGCGGCGATCGAGCAGGTCTGCCTGCTCTACCCGGTCGAC
CGCGACGCGGTCGGCCTGACCGGCATTTCCGCCGGCGCCGGCATGGCGGCGCTGCTCGCCACCTTGTACCCGACGCGCTT
TCGCGCCCTCGTGATGCATTCCGGCGTGCCGCCGGGCGTGGCGCATTCGCCGGCCACCGCGATGGCCGCGATGCACGGCA
GGCGCACGCCCGACTTCGCCACCGTGGGCGCGCAGCAATGGCCGCCGCTGCTGGTGATCCACGGCGACGCCGACCGCATC
GTCGCCAACGGCAATGCGCGCGCCGCGGTGCGGCTGTGGGCGGCCGCGCATGGCGCGCGTGAAGGCGAAACCCGGCGCCT
GCAGCGCGGCCGGCGCTACCCCGCCGTCGTCACCGACTACCACGCCGGCCGCCAACTGGTGGCAAGCCTGTACGAGGTGC
AAGGCCTCGGCCATGCCTGGAGCGGCGGCGCCCAGCGCCAGCCCTTCAGCGACCCGGCCGGACCCGACGCCTCGCGGCTG
GCGTGGTCCTTTCTGCAACGCCAGCTGCGGACGGCGGCGCGCAGCACGCCCGCCCCACGGACACGGGCACGCCCCGCCGT
ACTGGCCCGCCCCCGGAGCTAG

Upstream 100 bases:

>100_bases
CACCGGGCGACACCCGCCCGTAACCAGTCCCAACATTTTCACCGCCGGTCCGTCGCGCGTTAGGCATTAAGCTGGGGGCT
TCCCTTCCCCTGCCAGACCG

Downstream 100 bases:

>100_bases
CCATGCTGCATCTCGCCATCGTCGATGACCACGCGATCGTGCGCGCAGGCTTTCGCGAAATGCTTGCGACCGAAACCGAC
CTCCACATCGTGTTCGAGGC

Product: hypothetical protein

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MSRRTRKSPLGTAFQRTLRSLTRASVRAGTKALDQAARIAAEHARPPPGQGDWIAGQALGPAGVRRYYLFRPDGITRTQR
VPLMVMLHGCGQTARSFALSTRMNALAARERFLVLYPEQDRRANPQGCWEWFGTATRTGHAEAATLLAAIEQVCLLYPVD
RDAVGLTGISAGAGMAALLATLYPTRFRALVMHSGVPPGVAHSPATAMAAMHGRRTPDFATVGAQQWPPLLVIHGDADRI
VANGNARAAVRLWAAAHGAREGETRRLQRGRRYPAVVTDYHAGRQLVASLYEVQGLGHAWSGGAQRQPFSDPAGPDASRL
AWSFLQRQLRTAARSTPAPRTRARPAVLARPRS

Sequences:

>Translated_353_residues
MSRRTRKSPLGTAFQRTLRSLTRASVRAGTKALDQAARIAAEHARPPPGQGDWIAGQALGPAGVRRYYLFRPDGITRTQR
VPLMVMLHGCGQTARSFALSTRMNALAARERFLVLYPEQDRRANPQGCWEWFGTATRTGHAEAATLLAAIEQVCLLYPVD
RDAVGLTGISAGAGMAALLATLYPTRFRALVMHSGVPPGVAHSPATAMAAMHGRRTPDFATVGAQQWPPLLVIHGDADRI
VANGNARAAVRLWAAAHGAREGETRRLQRGRRYPAVVTDYHAGRQLVASLYEVQGLGHAWSGGAQRQPFSDPAGPDASRL
AWSFLQRQLRTAARSTPAPRTRARPAVLARPRS
>Mature_352_residues
SRRTRKSPLGTAFQRTLRSLTRASVRAGTKALDQAARIAAEHARPPPGQGDWIAGQALGPAGVRRYYLFRPDGITRTQRV
PLMVMLHGCGQTARSFALSTRMNALAARERFLVLYPEQDRRANPQGCWEWFGTATRTGHAEAATLLAAIEQVCLLYPVDR
DAVGLTGISAGAGMAALLATLYPTRFRALVMHSGVPPGVAHSPATAMAAMHGRRTPDFATVGAQQWPPLLVIHGDADRIV
ANGNARAAVRLWAAAHGAREGETRRLQRGRRYPAVVTDYHAGRQLVASLYEVQGLGHAWSGGAQRQPFSDPAGPDASRLA
WSFLQRQLRTAARSTPAPRTRARPAVLARPRS

Specific function: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P- nitrophenylbutyrate as substrates [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 38234; Mature: 38103

Theoretical pI: Translated: 12.10; Mature: 12.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRTRKSPLGTAFQRTLRSLTRASVRAGTKALDQAARIAAEHARPPPGQGDWIAGQALG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCC
PAGVRRYYLFRPDGITRTQRVPLMVMLHGCGQTARSFALSTRMNALAARERFLVLYPEQD
CHHHCEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCEEEEECCCC
RRANPQGCWEWFGTATRTGHAEAATLLAAIEQVCLLYPVDRDAVGLTGISAGAGMAALLA
CCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHH
TLYPTRFRALVMHSGVPPGVAHSPATAMAAMHGRRTPDFATVGAQQWPPLLVIHGDADRI
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCHHCCCCCCCCCEEEEECCCCEE
VANGNARAAVRLWAAAHGAREGETRRLQRGRRYPAVVTDYHAGRQLVASLYEVQGLGHAW
EECCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCCEEECHHHHHHHHHHHHHHHCCCCCC
SGGAQRQPFSDPAGPDASRLAWSFLQRQLRTAARSTPAPRTRARPAVLARPRS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRRTRKSPLGTAFQRTLRSLTRASVRAGTKALDQAARIAAEHARPPPGQGDWIAGQALG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCC
PAGVRRYYLFRPDGITRTQRVPLMVMLHGCGQTARSFALSTRMNALAARERFLVLYPEQD
CHHHCEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCEEEEECCCC
RRANPQGCWEWFGTATRTGHAEAATLLAAIEQVCLLYPVDRDAVGLTGISAGAGMAALLA
CCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHH
TLYPTRFRALVMHSGVPPGVAHSPATAMAAMHGRRTPDFATVGAQQWPPLLVIHGDADRI
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCHHCCCCCCCCCEEEEECCCCEE
VANGNARAAVRLWAAAHGAREGETRRLQRGRRYPAVVTDYHAGRQLVASLYEVQGLGHAW
EECCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCCEEECHHHHHHHHHHHHHHHCCCCCC
SGGAQRQPFSDPAGPDASRLAWSFLQRQLRTAARSTPAPRTRARPAVLARPRS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8269961 [H]