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The map label for this gene is nifV [H]

Identifier: 119896842

GI number: 119896842

Start: 582028

End: 583218

Strand: Reverse

Name: nifV [H]

Synonym: azo0551

Alternate gene names: 119896842

Gene position: 583218-582028 (Counterclockwise)

Preceding gene: 119896843

Following gene: 119896841

Centisome position: 13.33

GC content: 71.28

Gene sequence:

>1191_bases
ATGTCTGCACCAGTCACCATCGACGACACCACCCTGCGCGACGGCGAGCAATCGGCCGGCGTCGCCTTCACCCGCGCGGA
GAAGTGCGGCATCGCCCGCATCCTCGCCGAGATCGGCGTGCCGGAGCTGGAGATCGGCATCCCGGCGATGGGCAGCGAAG
AGTGCGGCGACATCCGCGCGATCGCCGACACGCTCGCCGACGGCGGCCACGACACCCGCCTGATCGTCTGGGGCCGCCTC
ACCAGCGCCGACATCGACGCCTGTCGCAGCCTGCCGGTGCAGATGCTGGAACTGTCGGTGCCGGTCTCCGACCAGCAAAT
CCGCCACAAGCTCGGCGCCACCCGTGACGAGGTGCTGGCGCGGATCGCGCGTTGGGTGCCGGTGGCGCGTGAGGCGGGCT
TCGATGTCGGCGTCGGCGGCGAAGACGCCTCGCGCGCCGACCCGGCCTTCCTCGCGGAGGTCATCCAGGCCGCCGAAGCC
GCCGGCGCGCGCCGTTTCCGCTTCGCCGACACGCTGGGCATCCTCGACCCCTTCGCCACCTTCGAGGCGATCCGCGCGCT
GCGCGCCGCTTCAAAGCTGGAAATCGAAATGCACGCGCATGACGACCTCGGGCTGGCCACCGCCAACACCCTGGCCGCGG
TGCGCGCCGGCGCCAGCCATGTGAACACCACGGTGAACGGGCTCGGCGAACGTGCCGGCAACGCGGCGCTGGAAGAAGTC
GCGCTTGGCCTGCGCCAGTTCCATGGCATGGGCGAGATCATCGACTTCACCCGCCTGCTCGACACCTCCGAAGCAGTCGC
GCGCGCCTCCGGCCGCCCGGTGGGCTGGCACAAGAGCGTGGTCGGCGAAGGCGTATTCACCCACGAAGCCGGCATCCACG
TGGATGGCCTGCTGAAGGACCCCGCCAACTACCAGGGCATCGACCCCGCGCTGCTCGGCCGCAGCCACCGCATGCTGCTC
GGCAAACACTCGGGCGGCCGCGGCGTCGCCGCCGCCTACGCCGGGCTGGGCATCGAACTCGACGCCGCCCGCACCGCCTG
CCTGCTGGCCCGCATCCGCGAGTTCACCTCGCGCACCAAGCGCACCCCGCAGCGCGAAGACCTGCTCGCGTTCTGGGAAG
AGCTGGAAGCCGGCAGCGCGCAGTGCGACGACCTGCTGCACGACCTGCTCGACACCGCCGCGGGAGCCTGA

Upstream 100 bases:

>100_bases
ACCTACGCCTAAGCCGCGCGCGCCGTCGCCACCCGCGACGGCGCCGCACCGGACCCGCCGCCTCCACCCGACGCGAACGA
CACCAGCCCAGGCCACGAGC

Downstream 100 bases:

>100_bases
CCATGAAGACCGAATCGATGAGCATGACCAACCCCGTCCGCGGCCCCGGCCTGTTCGCGCTGCTGCGCGAAGACGTGCGT
TGCGTGTTCGGCCGCGACCC

Product: homocitrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSAPVTIDDTTLRDGEQSAGVAFTRAEKCGIARILAEIGVPELEIGIPAMGSEECGDIRAIADTLADGGHDTRLIVWGRL
TSADIDACRSLPVQMLELSVPVSDQQIRHKLGATRDEVLARIARWVPVAREAGFDVGVGGEDASRADPAFLAEVIQAAEA
AGARRFRFADTLGILDPFATFEAIRALRAASKLEIEMHAHDDLGLATANTLAAVRAGASHVNTTVNGLGERAGNAALEEV
ALGLRQFHGMGEIIDFTRLLDTSEAVARASGRPVGWHKSVVGEGVFTHEAGIHVDGLLKDPANYQGIDPALLGRSHRMLL
GKHSGGRGVAAAYAGLGIELDAARTACLLARIREFTSRTKRTPQREDLLAFWEELEAGSAQCDDLLHDLLDTAAGA

Sequences:

>Translated_396_residues
MSAPVTIDDTTLRDGEQSAGVAFTRAEKCGIARILAEIGVPELEIGIPAMGSEECGDIRAIADTLADGGHDTRLIVWGRL
TSADIDACRSLPVQMLELSVPVSDQQIRHKLGATRDEVLARIARWVPVAREAGFDVGVGGEDASRADPAFLAEVIQAAEA
AGARRFRFADTLGILDPFATFEAIRALRAASKLEIEMHAHDDLGLATANTLAAVRAGASHVNTTVNGLGERAGNAALEEV
ALGLRQFHGMGEIIDFTRLLDTSEAVARASGRPVGWHKSVVGEGVFTHEAGIHVDGLLKDPANYQGIDPALLGRSHRMLL
GKHSGGRGVAAAYAGLGIELDAARTACLLARIREFTSRTKRTPQREDLLAFWEELEAGSAQCDDLLHDLLDTAAGA
>Mature_395_residues
SAPVTIDDTTLRDGEQSAGVAFTRAEKCGIARILAEIGVPELEIGIPAMGSEECGDIRAIADTLADGGHDTRLIVWGRLT
SADIDACRSLPVQMLELSVPVSDQQIRHKLGATRDEVLARIARWVPVAREAGFDVGVGGEDASRADPAFLAEVIQAAEAA
GARRFRFADTLGILDPFATFEAIRALRAASKLEIEMHAHDDLGLATANTLAAVRAGASHVNTTVNGLGERAGNAALEEVA
LGLRQFHGMGEIIDFTRLLDTSEAVARASGRPVGWHKSVVGEGVFTHEAGIHVDGLLKDPANYQGIDPALLGRSHRMLLG
KHSGGRGVAAAYAGLGIELDAARTACLLARIREFTSRTKRTPQREDLLAFWEELEAGSAQCDDLLHDLLDTAAGA

Specific function: This protein is a Fe-Mo-cofactor biosynthetic component [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=338, Percent_Identity=37.2781065088757, Blast_Score=180, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6320019, Length=352, Percent_Identity=25.2840909090909, Blast_Score=121, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320071, Length=352, Percent_Identity=25, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6324682, Length=372, Percent_Identity=23.6559139784946, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.14 [H]

Molecular weight: Translated: 41970; Mature: 41839

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPVTIDDTTLRDGEQSAGVAFTRAEKCGIARILAEIGVPELEIGIPAMGSEECGDIRA
CCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHH
IADTLADGGHDTRLIVWGRLTSADIDACRSLPVQMLELSVPVSDQQIRHKLGATRDEVLA
HHHHHHCCCCCCEEEEEECCCCHHHHHHHCCCHHHHHCCCCCCHHHHHHHHCCCHHHHHH
RIARWVPVAREAGFDVGVGGEDASRADPAFLAEVIQAAEAAGARRFRFADTLGILDPFAT
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHCHHHH
FEAIRALRAASKLEIEMHAHDDLGLATANTLAAVRAGASHVNTTVNGLGERAGNAALEEV
HHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
ALGLRQFHGMGEIIDFTRLLDTSEAVARASGRPVGWHKSVVGEGVFTHEAGIHVDGLLKD
HHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCEEECCCCEECCEECC
PANYQGIDPALLGRSHRMLLGKHSGGRGVAAAYAGLGIELDAARTACLLARIREFTSRTK
CCCCCCCCHHHHCCCCCEEEECCCCCCCHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHH
RTPQREDLLAFWEELEAGSAQCDDLLHDLLDTAAGA
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAPVTIDDTTLRDGEQSAGVAFTRAEKCGIARILAEIGVPELEIGIPAMGSEECGDIRA
CCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHH
IADTLADGGHDTRLIVWGRLTSADIDACRSLPVQMLELSVPVSDQQIRHKLGATRDEVLA
HHHHHHCCCCCCEEEEEECCCCHHHHHHHCCCHHHHHCCCCCCHHHHHHHHCCCHHHHHH
RIARWVPVAREAGFDVGVGGEDASRADPAFLAEVIQAAEAAGARRFRFADTLGILDPFAT
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHCHHHH
FEAIRALRAASKLEIEMHAHDDLGLATANTLAAVRAGASHVNTTVNGLGERAGNAALEEV
HHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
ALGLRQFHGMGEIIDFTRLLDTSEAVARASGRPVGWHKSVVGEGVFTHEAGIHVDGLLKD
HHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCEEECCCCEECCEECC
PANYQGIDPALLGRSHRMLLGKHSGGRGVAAAYAGLGIELDAARTACLLARIREFTSRTK
CCCCCCCCHHHHCCCCCEEEECCCCCCCHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHH
RTPQREDLLAFWEELEAGSAQCDDLLHDLLDTAAGA
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644218; 3040672 [H]