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The map label for this gene is nifL [H]

Identifier: 119896809

GI number: 119896809

Start: 553036

End: 554607

Strand: Direct

Name: nifL [H]

Synonym: azo0518

Alternate gene names: 119896809

Gene position: 553036-554607 (Clockwise)

Preceding gene: 119896788

Following gene: 119896810

Centisome position: 12.64

GC content: 67.88

Gene sequence:

>1572_bases
ATGCACGAGATCATCATGCCCGCACCCGTACCAACGCCCACCATCTTCGCTGACGCGATCTTCCACTACGCCGTGGAGCA
GTCGGCGATCGCGATCTCGATCACCGACCAGCACGCCAACATCCTCTATGTGAACCCCGCGTTCTGTGCGATCACCGGCT
ACGCCGCGCACGAGGTGGTCGGCCACAAGCAATCGCTGCTGTCATACAAATCCACCCCCAAGGTGGTCTACCAGGAACTG
TGGGGCGCGCTGAAAAAAGGCCGTTCGTGGACCGGGCGCCTGCTCAACCGCCGCCGCGACGGCTCGCCCTATGTGGCCGA
ACTCACCGTGACGCCGCTGAAGACGCCGGAGCATGAGGACGAGGACATCAACTACCTCGGCATGCACTGGGATGCCACCG
AGGAACACCGGCTGTCGCGCCAGCTCGCCAACCACAAGCAGCTGATCGAATCGGTGATTTCGGTCGCGCCGGTGGCGGTG
GCGCTGCTCGACGTCGACGGCCGGGTGGTGCTCGACAATCCGGCCTACAAGCGCATCGTCGCCGAGATGAAGGTCGACGA
GCCGGCGCATGCGGTGATCGAGTCGCTGCGCCACAACCTCGGCGACAGCTTCGAAAAGGCCTTTGCCAGCCGCCGCGCGC
TGCTCAACCAGGAGGTGCGGTTCGACCGCGCCGGTGGCGAGCCGCGCTGGTATGCCTGTTCGCTGTCGTGGTTCGAGGAA
CATCACACCAGTCCGGACGCGTTTTATGGCGACGGCTGTTCCGACTACATGCTGCTGGTGATGCACGATATCAGTGCGTC
GAAGCGCCAACAGGAGGCGCTGCGCCTCGCCGCGATGCGCGCGATGCTGTCCGAGGGCGAACTCAACCAGAGCCTGCGCG
AGGCGCTCGCGGGCGCGGTGTTCCAGTTGCAGGGCCCGGTCAACCTGATCTCCGCCGCCGCCGGGCTGCAGCGCCGCCGT
GCCGGTCCCGGCGCCGGCAGCGACCCGCTGGCGCGTGCGCTGTCCGAGGCGCAGCGCGCCGGCGAGGAGGCGATCGCCAC
GCTGCAGGCGGCGATGCCGCCCGAGCCGGAGGAGCCCTCCGGCCCGGTCAATCTCAATGAAGTGCTGCGCGACGTGCTGA
TGCTCGAAACCGACGCGCTGCTGTCGGCGGGCATCACCGTCGATTGGCATCCGGCCCACGTGCTGCCCACCGTGCAGGGC
GACCCCACGGCGCTGCGCACGCTGTTCCGCCAGCTCGTCTCCAACGCGATCGAGGCGATGAACGTGCGCGGCTGGAAGGA
GCGCGCGCTGGTGCTGCGCACGTCCACCCAGAACGGCCAGGTCGACGTGGAGGTGACCGATACCGGCCCGGGCATACCGG
AAGCCTTGCGGCTGAAAGTGTTCGAGCCCTTCTTCTCGACCAAGAAGGCGCGCGCCGCCGGCCGCGGCGTCGGCCTGTCG
CTGGCGCAGGAAATCGTCACCCGCCATCGCGGCATGCTCGAGATCGACCCTGAGTACAGCGGCGGCTGCCGCCTGCGCAT
CAGCTTTCCGGCGCTGCGCTCGGCCCCGCTCGACCTGGAGGGCGGTCGATGA

Upstream 100 bases:

>100_bases
CAGGTCGGAGGCGAGGATGGGCGCTGTCGCCGACGCACCGTGGCGGTGCGAACAAATGTCGCATTTGGCACAACGCTTGC
TACAGGACGGGTGGCCCGCA

Downstream 100 bases:

>100_bases
GCGCGGCCGGTCCGATGCCCGCCGCCGAGGAGCGCGTCGCCTGGCTGGAGGCCACGCTGGAGGCGCTGTACCGCGTCAGC
CGCGTGCTGTCGCGCTCGCT

Product: nitrogen fixation regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MHEIIMPAPVPTPTIFADAIFHYAVEQSAIAISITDQHANILYVNPAFCAITGYAAHEVVGHKQSLLSYKSTPKVVYQEL
WGALKKGRSWTGRLLNRRRDGSPYVAELTVTPLKTPEHEDEDINYLGMHWDATEEHRLSRQLANHKQLIESVISVAPVAV
ALLDVDGRVVLDNPAYKRIVAEMKVDEPAHAVIESLRHNLGDSFEKAFASRRALLNQEVRFDRAGGEPRWYACSLSWFEE
HHTSPDAFYGDGCSDYMLLVMHDISASKRQQEALRLAAMRAMLSEGELNQSLREALAGAVFQLQGPVNLISAAAGLQRRR
AGPGAGSDPLARALSEAQRAGEEAIATLQAAMPPEPEEPSGPVNLNEVLRDVLMLETDALLSAGITVDWHPAHVLPTVQG
DPTALRTLFRQLVSNAIEAMNVRGWKERALVLRTSTQNGQVDVEVTDTGPGIPEALRLKVFEPFFSTKKARAAGRGVGLS
LAQEIVTRHRGMLEIDPEYSGGCRLRISFPALRSAPLDLEGGR

Sequences:

>Translated_523_residues
MHEIIMPAPVPTPTIFADAIFHYAVEQSAIAISITDQHANILYVNPAFCAITGYAAHEVVGHKQSLLSYKSTPKVVYQEL
WGALKKGRSWTGRLLNRRRDGSPYVAELTVTPLKTPEHEDEDINYLGMHWDATEEHRLSRQLANHKQLIESVISVAPVAV
ALLDVDGRVVLDNPAYKRIVAEMKVDEPAHAVIESLRHNLGDSFEKAFASRRALLNQEVRFDRAGGEPRWYACSLSWFEE
HHTSPDAFYGDGCSDYMLLVMHDISASKRQQEALRLAAMRAMLSEGELNQSLREALAGAVFQLQGPVNLISAAAGLQRRR
AGPGAGSDPLARALSEAQRAGEEAIATLQAAMPPEPEEPSGPVNLNEVLRDVLMLETDALLSAGITVDWHPAHVLPTVQG
DPTALRTLFRQLVSNAIEAMNVRGWKERALVLRTSTQNGQVDVEVTDTGPGIPEALRLKVFEPFFSTKKARAAGRGVGLS
LAQEIVTRHRGMLEIDPEYSGGCRLRISFPALRSAPLDLEGGR
>Mature_523_residues
MHEIIMPAPVPTPTIFADAIFHYAVEQSAIAISITDQHANILYVNPAFCAITGYAAHEVVGHKQSLLSYKSTPKVVYQEL
WGALKKGRSWTGRLLNRRRDGSPYVAELTVTPLKTPEHEDEDINYLGMHWDATEEHRLSRQLANHKQLIESVISVAPVAV
ALLDVDGRVVLDNPAYKRIVAEMKVDEPAHAVIESLRHNLGDSFEKAFASRRALLNQEVRFDRAGGEPRWYACSLSWFEE
HHTSPDAFYGDGCSDYMLLVMHDISASKRQQEALRLAAMRAMLSEGELNQSLREALAGAVFQLQGPVNLISAAAGLQRRR
AGPGAGSDPLARALSEAQRAGEEAIATLQAAMPPEPEEPSGPVNLNEVLRDVLMLETDALLSAGITVDWHPAHVLPTVQG
DPTALRTLFRQLVSNAIEAMNVRGWKERALVLRTSTQNGQVDVEVTDTGPGIPEALRLKVFEPFFSTKKARAAGRGVGLS
LAQEIVTRHRGMLEIDPEYSGGCRLRISFPALRSAPLDLEGGR

Specific function: Required for the inhibition of nifA activity in response to oxygen and low level of fixed nitrogen [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=365, Percent_Identity=23.8356164383562, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790436, Length=216, Percent_Identity=27.3148148148148, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789808, Length=216, Percent_Identity=27.7777777777778, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1789453, Length=104, Percent_Identity=28.8461538461538, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1790346, Length=228, Percent_Identity=25.4385964912281, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR014285
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR005467 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 57437; Mature: 57437

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHEIIMPAPVPTPTIFADAIFHYAVEQSAIAISITDQHANILYVNPAFCAITGYAAHEVV
CCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCEEEECCHHHEEECHHHHHHH
GHKQSLLSYKSTPKVVYQELWGALKKGRSWTGRLLNRRRDGSPYVAELTVTPLKTPEHED
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEEEEECCCCCCCCC
EDINYLGMHWDATEEHRLSRQLANHKQLIESVISVAPVAVALLDVDGRVVLDNPAYKRIV
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEECCHHHHHHH
AEMKVDEPAHAVIESLRHNLGDSFEKAFASRRALLNQEVRFDRAGGEPRWYACSLSWFEE
HHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHH
HHTSPDAFYGDGCSDYMLLVMHDISASKRQQEALRLAAMRAMLSEGELNQSLREALAGAV
HCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
FQLQGPVNLISAAAGLQRRRAGPGAGSDPLARALSEAQRAGEEAIATLQAAMPPEPEEPS
EEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCC
GPVNLNEVLRDVLMLETDALLSAGITVDWHPAHVLPTVQGDPTALRTLFRQLVSNAIEAM
CCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHH
NVRGWKERALVLRTSTQNGQVDVEVTDTGPGIPEALRLKVFEPFFSTKKARAAGRGVGLS
CCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
LAQEIVTRHRGMLEIDPEYSGGCRLRISFPALRSAPLDLEGGR
HHHHHHHHCCCCEEECCCCCCCEEEEEECCHHCCCCCCCCCCC
>Mature Secondary Structure
MHEIIMPAPVPTPTIFADAIFHYAVEQSAIAISITDQHANILYVNPAFCAITGYAAHEVV
CCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCEEEECCHHHEEECHHHHHHH
GHKQSLLSYKSTPKVVYQELWGALKKGRSWTGRLLNRRRDGSPYVAELTVTPLKTPEHED
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEEEEECCCCCCCCC
EDINYLGMHWDATEEHRLSRQLANHKQLIESVISVAPVAVALLDVDGRVVLDNPAYKRIV
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEECCHHHHHHH
AEMKVDEPAHAVIESLRHNLGDSFEKAFASRRALLNQEVRFDRAGGEPRWYACSLSWFEE
HHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHH
HHTSPDAFYGDGCSDYMLLVMHDISASKRQQEALRLAAMRAMLSEGELNQSLREALAGAV
HCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
FQLQGPVNLISAAAGLQRRRAGPGAGSDPLARALSEAQRAGEEAIATLQAAMPPEPEEPS
EEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCC
GPVNLNEVLRDVLMLETDALLSAGITVDWHPAHVLPTVQGDPTALRTLFRQLVSNAIEAM
CCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHH
NVRGWKERALVLRTSTQNGQVDVEVTDTGPGIPEALRLKVFEPFFSTKKARAAGRGVGLS
CCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
LAQEIVTRHRGMLEIDPEYSGGCRLRISFPALRSAPLDLEGGR
HHHHHHHHCCCCEEECCCCCCCEEEEEECCHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8231815; 2840552; 9601070; 9593306 [H]