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The map label for this gene is rnfA1

Identifier: 119896808

GI number: 119896808

Start: 552106

End: 552687

Strand: Reverse

Name: rnfA1

Synonym: azo0517

Alternate gene names: 119896808

Gene position: 552687-552106 (Counterclockwise)

Preceding gene: 119896811

Following gene: 119896807

Centisome position: 12.63

GC content: 64.26

Gene sequence:

>582_bases
ATGAGCGAATGGTTGATGCTGCTGCTGGGGACCGCCCTGGTAAATAACGTGGTGCTGGTCAAGTTCCTGGGCCTGTGCCC
CTTCATGGGGGTGTCCAAGAAGGTCGACAGCGCGATCGGCATGGGCATGGCCACCACTTTCGTGCTGACGCTCGCAAGCG
CGCTGACCTGGCTGATCGAACACTTCCTGCTGGTGCCGTTCGACTTCGGCTACCTGCGCATCCTGTCCTTCATCCTGGTG
ATCGCGGCGACGGTGCAGTTCGTCGAGATGGTCATCAAGAAGACCGCGCCCGACCTCTACAAGGTGCTGGGCATCTACCT
GCCGCTGATCACCACCAACTGCGCGGTGCTCGGCGTCGCGCTGCTCAATGCCGGCGAAGGCGCCGGCTTCGTCCGCAGCG
TGCTGTACGGCTTCGGCTCGGCGCTCGGCTTCACGATGGTGATGGTGCTGTTCGCCGGTCTGCGCGAACGCCTTGCCCTC
ACCTCGGTGCCGGCGGCCTTCTCCGGCGCGCCGATCAGCTTCATCACCGCCGGCCTGCTGTCGCTCGCCTTCATGGGCTT
TGCCGGCCTCACCAACCACTGA

Upstream 100 bases:

>100_bases
CACCATTTCTTTTTATTCAACAACTTGCGTCGAAAATCAGCGTGGCACGCGTTCTGCAAAGTGCTCCCCACACCCATGCA
TTGGGCGAGTGAGGAAAGCG

Downstream 100 bases:

>100_bases
ATCCCGGGAGCACGAAGATGTTGATCGCAGTTACCAGCGTCGCCCTGATCGGGGCCTTCTGTGGCGTGGCGCTCGGCGTC
GCGGCGCGCCGCTTCTACGT

Product: electron transport complex protein RnfA

Products: NA

Alternate protein names: Nitrogen fixation protein rnfA

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MSEWLMLLLGTALVNNVVLVKFLGLCPFMGVSKKVDSAIGMGMATTFVLTLASALTWLIEHFLLVPFDFGYLRILSFILV
IAATVQFVEMVIKKTAPDLYKVLGIYLPLITTNCAVLGVALLNAGEGAGFVRSVLYGFGSALGFTMVMVLFAGLRERLAL
TSVPAAFSGAPISFITAGLLSLAFMGFAGLTNH

Sequences:

>Translated_193_residues
MSEWLMLLLGTALVNNVVLVKFLGLCPFMGVSKKVDSAIGMGMATTFVLTLASALTWLIEHFLLVPFDFGYLRILSFILV
IAATVQFVEMVIKKTAPDLYKVLGIYLPLITTNCAVLGVALLNAGEGAGFVRSVLYGFGSALGFTMVMVLFAGLRERLAL
TSVPAAFSGAPISFITAGLLSLAFMGFAGLTNH
>Mature_192_residues
SEWLMLLLGTALVNNVVLVKFLGLCPFMGVSKKVDSAIGMGMATTFVLTLASALTWLIEHFLLVPFDFGYLRILSFILVI
AATVQFVEMVIKKTAPDLYKVLGIYLPLITTNCAVLGVALLNAGEGAGFVRSVLYGFGSALGFTMVMVLFAGLRERLALT
SVPAAFSGAPISFITAGLLSLAFMGFAGLTNH

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase

COG id: COG4657

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family

Homologues:

Organism=Escherichia coli, GI1787914, Length=192, Percent_Identity=65.625, Blast_Score=257, Evalue=3e-70,
Organism=Escherichia coli, GI1787919, Length=156, Percent_Identity=38.4615384615385, Blast_Score=74, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNFA_AZOSB (A1K2S9)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932021.1
- STRING:   A1K2S9
- GeneID:   4609742
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo0517
- eggNOG:   COG4657
- HOGENOM:   HBG624915
- OMA:   VTTRVDT
- PhylomeDB:   A1K2S9
- BioCyc:   ASP62928:AZO0517-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00459
- InterPro:   IPR003667
- InterPro:   IPR011293
- PIRSF:   PIRSF006102
- TIGRFAMs:   TIGR01943

Pfam domain/function: PF02508 Rnf-Nqr

EC number: NA

Molecular weight: Translated: 20536; Mature: 20405

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x19561d9c)-; HASH(0x197dcf74)-; HASH(0x19a7ff5c)-; HASH(0x19540248)-; HASH(0x19b9e10c)-; HASH(0xdf8d168)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEWLMLLLGTALVNNVVLVKFLGLCPFMGVSKKVDSAIGMGMATTFVLTLASALTWLIE
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HFLLVPFDFGYLRILSFILVIAATVQFVEMVIKKTAPDLYKVLGIYLPLITTNCAVLGVA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LLNAGEGAGFVRSVLYGFGSALGFTMVMVLFAGLRERLALTSVPAAFSGAPISFITAGLL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
SLAFMGFAGLTNH
HHHHHHHHCCCCH
>Mature Secondary Structure 
SEWLMLLLGTALVNNVVLVKFLGLCPFMGVSKKVDSAIGMGMATTFVLTLASALTWLIE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HFLLVPFDFGYLRILSFILVIAATVQFVEMVIKKTAPDLYKVLGIYLPLITTNCAVLGVA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LLNAGEGAGFVRSVLYGFGSALGFTMVMVLFAGLRERLALTSVPAAFSGAPISFITAGLL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
SLAFMGFAGLTNH
HHHHHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA