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The map label for this gene is rpoN1 [H]

Identifier: 119896795

GI number: 119896795

Start: 541320

End: 542780

Strand: Reverse

Name: rpoN1 [H]

Synonym: azo0504

Alternate gene names: 119896795

Gene position: 542780-541320 (Counterclockwise)

Preceding gene: 119896796

Following gene: 119896794

Centisome position: 12.4

GC content: 65.57

Gene sequence:

>1461_bases
ATGAAGCCCACGCTCCAACTCAAGCTCTCCCAGCACCTGACGCTGACGCCGCAGTTGCAGCAGTCGATCAAGCTGCTGCA
GCTCTCCACGCTCGAACTCAACACCGAGATCGAACGCTTCCTGCTCGAAAACCCGATGCTCGAGCGCGAGGAGATGGACG
GCGGCGGGCACGACTTCCAGGCGCAGCTCTCTTCCGGCACCACCACGGCCTCGACCACGACCGAAAGCAGTGGCGAAAGT
GGCGGCGAAGCGGAGTCGCGCGCCGACAGCGCGGAAAGCGGCAGCTTCGACGAGGGCATGGACTGGTCGTCCGGCTCGGG
CAGCGGCTCGTCGAGCAACCGCGACGACGATGATGACGTCGATTTTCAGGAGTTCCAGGCCGCGGGCATCTCGCTGCGCG
ACCATCTCGACCAGCAGGTCGCGCTGTCTCCGCTGTCCGACCGCGACCGCGCGCTGGTGCGCTTCCTGATCGAGGCGCTC
GACGACGACGGCTACCTGCACCAGGAGCTGGAAGAGTTGCTCGAGCTGCTGCCGCCCGAACTCGATGTCGAACTCGACGA
CCTCTGCATCGCGCTGAAGCATGTGCAAAGCCTCGAGCCGGCCGGCATCGGCGCCCGCACCCCGCAGGAATGCCTCGCGC
TGCAGCTGCGCGCGATGCCGGCCGAGCCGGTACGCGACCTCGCCCTGCAGATCGTCAGCCAGCACCTCGAACTGCTCGCC
GAGCGCAACTTCGCCAAACTCAAGAAACTCACCGGTTGCGACGACGACGCGCTGCGCGCCGCCCACGCGCTGATCTGCCG
GCTCGATCCGCATCCCGGCGCGCAGCATTCGGTCGAGGAAACCCGCTACGTCCTGCCCGACGTCGTGGTGCGCAAGCTGC
GCGGGCGCTGGACCGTGCAACTCAACCCGGAAGCGATGCCGCGCCTGCGCATCAACCAGCTCTACGCCAGCATCCTGCAG
CAGAACCGGGGCCAGGGCGGCGGCCTGACCAGCCAGCTGCAGGAGGCGCGCTGGCTGATCAAGAATGTGCAGCAGCGCTT
CGACACCATCCTGCGTGTCTCCCAGGCCATCGTTGACCAGCAACGGCAGTTCTTCGATCATGGGGAGGTGGCCATGCGGC
CGCTTACACTCCGGGAGATCGCAGACCAGCTCGAGCTGCACGAATCCACCGTATCGCGCGTCACCACCCAGAAGTTCATG
GCGACCCCGCGCGGCGTGTTCGAACTCAAGTATTTCTTCGGCAGCCATGTCGCCACCGATACCGGTGGCGCGGCCTCCTC
CACGGCGATTCGCGCGCTGATTCGCCAGTTGGTGGATGCCGAGGACAGGAAGAAACCCCTGTCCGACGCCCGGATTGCCG
AATTGTTGGGCCAGCAGGGTATCGTGGTCGCCAGGCGGACCATCGCCAAGTATCGCGAATCGCTCAATATCCCGCCGGTC
AGTCTGCGCAAAACGATCTAA

Upstream 100 bases:

>100_bases
AGGGCGCGTCCTGGCTAGCGGCCAGCCGCGGGAAATCATCGACAACGAGAAGGTCCGCCAGGTGTACCTGGGCGAACACT
TCCGCCTCTGACCGCCCATC

Downstream 100 bases:

>100_bases
AAAGAGGACTCATCATGAATCTCAACATCACCGGGCATCACGTCGAAGTCACGCCGGCGATCCGCGAATACGTCGTGACC
AAGCTCGATCGCGTGATCGC

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MKPTLQLKLSQHLTLTPQLQQSIKLLQLSTLELNTEIERFLLENPMLEREEMDGGGHDFQAQLSSGTTTASTTTESSGES
GGEAESRADSAESGSFDEGMDWSSGSGSGSSSNRDDDDDVDFQEFQAAGISLRDHLDQQVALSPLSDRDRALVRFLIEAL
DDDGYLHQELEELLELLPPELDVELDDLCIALKHVQSLEPAGIGARTPQECLALQLRAMPAEPVRDLALQIVSQHLELLA
ERNFAKLKKLTGCDDDALRAAHALICRLDPHPGAQHSVEETRYVLPDVVVRKLRGRWTVQLNPEAMPRLRINQLYASILQ
QNRGQGGGLTSQLQEARWLIKNVQQRFDTILRVSQAIVDQQRQFFDHGEVAMRPLTLREIADQLELHESTVSRVTTQKFM
ATPRGVFELKYFFGSHVATDTGGAASSTAIRALIRQLVDAEDRKKPLSDARIAELLGQQGIVVARRTIAKYRESLNIPPV
SLRKTI

Sequences:

>Translated_486_residues
MKPTLQLKLSQHLTLTPQLQQSIKLLQLSTLELNTEIERFLLENPMLEREEMDGGGHDFQAQLSSGTTTASTTTESSGES
GGEAESRADSAESGSFDEGMDWSSGSGSGSSSNRDDDDDVDFQEFQAAGISLRDHLDQQVALSPLSDRDRALVRFLIEAL
DDDGYLHQELEELLELLPPELDVELDDLCIALKHVQSLEPAGIGARTPQECLALQLRAMPAEPVRDLALQIVSQHLELLA
ERNFAKLKKLTGCDDDALRAAHALICRLDPHPGAQHSVEETRYVLPDVVVRKLRGRWTVQLNPEAMPRLRINQLYASILQ
QNRGQGGGLTSQLQEARWLIKNVQQRFDTILRVSQAIVDQQRQFFDHGEVAMRPLTLREIADQLELHESTVSRVTTQKFM
ATPRGVFELKYFFGSHVATDTGGAASSTAIRALIRQLVDAEDRKKPLSDARIAELLGQQGIVVARRTIAKYRESLNIPPV
SLRKTI
>Mature_486_residues
MKPTLQLKLSQHLTLTPQLQQSIKLLQLSTLELNTEIERFLLENPMLEREEMDGGGHDFQAQLSSGTTTASTTTESSGES
GGEAESRADSAESGSFDEGMDWSSGSGSGSSSNRDDDDDVDFQEFQAAGISLRDHLDQQVALSPLSDRDRALVRFLIEAL
DDDGYLHQELEELLELLPPELDVELDDLCIALKHVQSLEPAGIGARTPQECLALQLRAMPAEPVRDLALQIVSQHLELLA
ERNFAKLKKLTGCDDDALRAAHALICRLDPHPGAQHSVEETRYVLPDVVVRKLRGRWTVQLNPEAMPRLRINQLYASILQ
QNRGQGGGLTSQLQEARWLIKNVQQRFDTILRVSQAIVDQQRQFFDHGEVAMRPLTLREIADQLELHESTVSRVTTQKFM
ATPRGVFELKYFFGSHVATDTGGAASSTAIRALIRQLVDAEDRKKPLSDARIAELLGQQGIVVARRTIAKYRESLNIPPV
SLRKTI

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=491, Percent_Identity=45.213849287169, Blast_Score=404, Evalue=1e-114,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 54035; Mature: 54035

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPTLQLKLSQHLTLTPQLQQSIKLLQLSTLELNTEIERFLLENPMLEREEMDGGGHDFQ
CCCCEEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCHH
AQLSSGTTTASTTTESSGESGGEAESRADSAESGSFDEGMDWSSGSGSGSSSNRDDDDDV
HHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DFQEFQAAGISLRDHLDQQVALSPLSDRDRALVRFLIEALDDDGYLHQELEELLELLPPE
CHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
LDVELDDLCIALKHVQSLEPAGIGARTPQECLALQLRAMPAEPVRDLALQIVSQHLELLA
CCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
ERNFAKLKKLTGCDDDALRAAHALICRLDPHPGAQHSVEETRYVLPDVVVRKLRGRWTVQ
HCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEE
LNPEAMPRLRINQLYASILQQNRGQGGGLTSQLQEARWLIKNVQQRFDTILRVSQAIVDQ
ECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRQFFDHGEVAMRPLTLREIADQLELHESTVSRVTTQKFMATPRGVFELKYFFGSHVATD
HHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCC
TGGAASSTAIRALIRQLVDAEDRKKPLSDARIAELLGQQGIVVARRTIAKYRESLNIPPV
CCCCHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
SLRKTI
HHCCCC
>Mature Secondary Structure
MKPTLQLKLSQHLTLTPQLQQSIKLLQLSTLELNTEIERFLLENPMLEREEMDGGGHDFQ
CCCCEEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCHH
AQLSSGTTTASTTTESSGESGGEAESRADSAESGSFDEGMDWSSGSGSGSSSNRDDDDDV
HHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DFQEFQAAGISLRDHLDQQVALSPLSDRDRALVRFLIEALDDDGYLHQELEELLELLPPE
CHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
LDVELDDLCIALKHVQSLEPAGIGARTPQECLALQLRAMPAEPVRDLALQIVSQHLELLA
CCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
ERNFAKLKKLTGCDDDALRAAHALICRLDPHPGAQHSVEETRYVLPDVVVRKLRGRWTVQ
HCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEE
LNPEAMPRLRINQLYASILQQNRGQGGGLTSQLQEARWLIKNVQQRFDTILRVSQAIVDQ
ECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRQFFDHGEVAMRPLTLREIADQLELHESTVSRVTTQKFMATPRGVFELKYFFGSHVATD
HHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCC
TGGAASSTAIRALIRQLVDAEDRKKPLSDARIAELLGQQGIVVARRTIAKYRESLNIPPV
CCCCHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
SLRKTI
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1417413 [H]