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The map label for this gene is ptsN [H]

Identifier: 119896793

GI number: 119896793

Start: 540363

End: 540848

Strand: Reverse

Name: ptsN [H]

Synonym: azo0502

Alternate gene names: 119896793

Gene position: 540848-540363 (Counterclockwise)

Preceding gene: 119896794

Following gene: 119896792

Centisome position: 12.36

GC content: 66.67

Gene sequence:

>486_bases
ATGAGCCTGATCGCCCAACTCCTGCCCCTTTCCAATGTCGTCGTCGATCTCGACGCGAGCAGCAAGAAACGGGTTTTCGA
GCACGCCGGCCTGCTGTTCGAAAACAACCAGGGCATCGCCCGCAGCACCGTATTCGACAGCCTGTTCTCCCGGGAACGGC
TGGGTTCCACCGGACTGGGTCAGGGTATCGCCATCCCGCACGGCCGCATCAAGGGCCTGAAGGAAGCCGCCGGCGCGTTC
CTGCGCCTGAGCGCTCCGGTCCAGTTCGACGCGCCCGACGGCCGCCCGGTGAACCTGCTGTTCGTGCTGCTGGTGCCCGA
GCAGGCCAACGAGACCCACCTGCAGTTGCTGTCGGAACTCGCGCAGATGTTCAGCGAGCGCGCCTTCCGCGAACAGCTGC
TGGTCGCCCCGGACGCCGCCACCATCCACGCGCTGTTCGCCAACTGGGGGCCCGATGCGGCAGACCAGCGTCGCGCGGCT
GTATGA

Upstream 100 bases:

>100_bases
TATAATCCGCGCCGTTCCGACGACGCCCGCCCGCATCCGCACCGTCCTCCCGCTCCCCGCGCCGCCAGTCCTCCCCGGCC
GCGCAAGCCCTCTCCCGCGC

Downstream 100 bases:

>100_bases
GGACCAGGGTCCCCGGCTGCAACTGACCCACGTCAGCGGGCCGCTCGACGCTGTCCTGTCCGTCGCTGAAGAACGCATGT
GGCCGGCCGACCTCGTCGGC

Product: protein-Npi-phosphohistidine-sugar phosphotransferase

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 161; Mature: 160

Protein sequence:

>161_residues
MSLIAQLLPLSNVVVDLDASSKKRVFEHAGLLFENNQGIARSTVFDSLFSRERLGSTGLGQGIAIPHGRIKGLKEAAGAF
LRLSAPVQFDAPDGRPVNLLFVLLVPEQANETHLQLLSELAQMFSERAFREQLLVAPDAATIHALFANWGPDAADQRRAA
V

Sequences:

>Translated_161_residues
MSLIAQLLPLSNVVVDLDASSKKRVFEHAGLLFENNQGIARSTVFDSLFSRERLGSTGLGQGIAIPHGRIKGLKEAAGAF
LRLSAPVQFDAPDGRPVNLLFVLLVPEQANETHLQLLSELAQMFSERAFREQLLVAPDAATIHALFANWGPDAADQRRAA
V
>Mature_160_residues
SLIAQLLPLSNVVVDLDASSKKRVFEHAGLLFENNQGIARSTVFDSLFSRERLGSTGLGQGIAIPHGRIKGLKEAAGAFL
RLSAPVQFDAPDGRPVNLLFVLLVPEQANETHLQLLSELAQMFSERAFREQLLVAPDAATIHALFANWGPDAADQRRAAV

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=139, Percent_Identity=36.6906474820144, Blast_Score=97, Evalue=6e-22,
Organism=Escherichia coli, GI1786951, Length=91, Percent_Identity=39.5604395604396, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1788726, Length=141, Percent_Identity=29.0780141843972, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 17454; Mature: 17323

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLIAQLLPLSNVVVDLDASSKKRVFEHAGLLFENNQGIARSTVFDSLFSRERLGSTGLG
CCHHHHHCCCCCEEEECCCCCHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCC
QGIAIPHGRIKGLKEAAGAFLRLSAPVQFDAPDGRPVNLLFVLLVPEQANETHLQLLSEL
CCEECCCHHHHHHHHHCCCEEEECCCEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHHH
AQMFSERAFREQLLVAPDAATIHALFANWGPDAADQRRAAV
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHCCCC
>Mature Secondary Structure 
SLIAQLLPLSNVVVDLDASSKKRVFEHAGLLFENNQGIARSTVFDSLFSRERLGSTGLG
CHHHHHCCCCCEEEECCCCCHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCC
QGIAIPHGRIKGLKEAAGAFLRLSAPVQFDAPDGRPVNLLFVLLVPEQANETHLQLLSEL
CCEECCCHHHHHHHHHCCCEEEECCCEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHHH
AQMFSERAFREQLLVAPDAATIHALFANWGPDAADQRRAAV
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]