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The map label for this gene is kefB [H]

Identifier: 119896740

GI number: 119896740

Start: 478380

End: 480146

Strand: Reverse

Name: kefB [H]

Synonym: azo0449

Alternate gene names: 119896740

Gene position: 480146-478380 (Counterclockwise)

Preceding gene: 119896742

Following gene: 119896739

Centisome position: 10.97

GC content: 69.78

Gene sequence:

>1767_bases
ATGCACCACTTCAGCGCGTCGCGCTACCGCATGGACCACGAAGCGTTTCCCTTCCTGAAAGAGACGATCCTCTTTCTCGC
CCTCTCGGGGGTGCTGATTCCGCTGCTCGCCCGCCTGCGCATCAATCCGGTGCTCGGTTTTCTCGCCGTCGGCACCGCGC
TCGGGCCATACGGGCTAGCGAGTTTCGTGCCGGCGCACCCGTGGCTGGCCTACGTCACCTTTCCGCGCCAGGAAGAGGTG
GAAACGCTGGCCGAACTCGGCGTGATCTTCCTGATGTTCATGATCGGCCTCGAACTCTCGGTGGACCGGCTGTGGTCGCT
GCGGCGCCAGGTGTTCGGGCTCGGCACGCTGCAGGTGGCGCTGACCGCGGCGCTGATCGGCGGCATCGCCCATCTGTTCG
GCAACACCACCGAAGCCTCGGTGATCCTCGGCCTGGTGCTGTCCTTCTCGTCCACTGCCATCGTCATGCAGTTGCTGATC
CAGCGCGGCGAGCTGGGCGGCCCGCTCGGCCAGGCGGCGTTCGCGATCCTGCTGCTGCAGGATCTGGCCGTGGTGCCGCT
GCTGGTGCTGCTCAGCATCCTCGGCGCCGGCAACAGCGAGGGCAGCTTCGCGCTGCTGCTCGGCACCGCCGCGGCCAAGG
GGCTGGTAACGGTGGGGCTGATCTACCTGATCGGGCGCCGCCTGGTGCGGCCGGTGTTCCACCAGATCACCGTCAGCCGC
CAGCCCGACACCTTCATGGCGCTGACGCTGCTGACCACGCTTGGCGTCGCCGCGATGACCTGGGCAGCCGGCCTGTCGAT
GGCGCTCGGCGCGCTGCTGGCCGGCCTGATCATCGCCGAGACCGAGTTCCGCCACGAGGTCGAGGTCACGATCGAGCCCT
TCAAGGGCCTGCTGATGGGGCTGTTCTTCCTGTCGGTGGGCATGGGCATCGACCTGCGCGCGCTGCTTGCCGAACCGCTG
TGGATCCCGCTCGCGGTGGCCGGGCTGATGATCATCAAGGGCGCGGTCGTGTTCGCGCTGCTGCGCCTGTTCGGGCTGTC
ATGGGGGCGCGCGGTCGAAGGCGGCTTCCTGCTCGGCCAGGGGGGCGAGTTCGCCTTCATCGTGATCGGCCTCGCGCTGT
CCTTCAAGCTGCTGCCGCGCGACGTCGGCCAGTTCATGCTGCTGGCGGTGGGCCTGTCGATGCTCGCAACGCCGGTGGTG
GCGCGGCTCGGGCGCGAGCTGGGGGCCGCGATGGACCGCCGCCAGCCGCCGGCGGCCCAGGAGGACGCCGAGTTCGGCAC
CCTGCGCGGCCATGTGCTGATCGCCGGCTATGGCCGCGTCGGCCAGATGGTGGGGCAGATGCTGGCCGAACAGGGCGTGC
CGCACGTGGCCGTGGAAACCGACGCCAAGCTGGTGGCCGGCCAGCGCAAGACCGGCGCCGCGGTGATCTACGGCGACGCC
AGCCGCCCCGAGCTGCTGCGCAAGCTGCACCTCGACCGCGCGCTCGCGGTGGTGCTGACGATGGACCACACCGCCGCCGC
GGTGCACGCGGTGAAGGGCATCCGCCAGAGCAATCCGCACGTCCGCATCATCGCCCGCGCGCGCGACGAGAAACACGCGC
TGACCCTGCGCGAGGCCGGCGCATCGGTGGTGGTGCCGGAAACCCTGGAATCCGGGCTCAAGCTCACCGGCTCGGTACTC
GAAACCCTGGGCGTGCCCGCCGACGCCGCCAGCCGCCTGCTGGAGCAGGAACGTGACCGCCGCATCGTCGCCTTCCGCGA
AAGCTGA

Upstream 100 bases:

>100_bases
AGCTCCTGCAAGGGGGTTCATGACATAGCCGTCATTTTAGGCATCGCAAGCTCCGCTTGTCTGCGACAGGCGCGAGCGCG
GGTGCCGATCCATTACACTC

Downstream 100 bases:

>100_bases
CTCCGCGGCCGAGGCGGTTCCCCGCCCGGCGCCGGAGCGTCCGCGGCCGGGCCACGGGCCGCTGCTCGCCGCGCTGGCGG
TGCTGCTGGTCAGCCTGGCC

Product: putative glutathione-regulated potassium-efflux system protein

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 588; Mature: 588

Protein sequence:

>588_residues
MHHFSASRYRMDHEAFPFLKETILFLALSGVLIPLLARLRINPVLGFLAVGTALGPYGLASFVPAHPWLAYVTFPRQEEV
ETLAELGVIFLMFMIGLELSVDRLWSLRRQVFGLGTLQVALTAALIGGIAHLFGNTTEASVILGLVLSFSSTAIVMQLLI
QRGELGGPLGQAAFAILLLQDLAVVPLLVLLSILGAGNSEGSFALLLGTAAAKGLVTVGLIYLIGRRLVRPVFHQITVSR
QPDTFMALTLLTTLGVAAMTWAAGLSMALGALLAGLIIAETEFRHEVEVTIEPFKGLLMGLFFLSVGMGIDLRALLAEPL
WIPLAVAGLMIIKGAVVFALLRLFGLSWGRAVEGGFLLGQGGEFAFIVIGLALSFKLLPRDVGQFMLLAVGLSMLATPVV
ARLGRELGAAMDRRQPPAAQEDAEFGTLRGHVLIAGYGRVGQMVGQMLAEQGVPHVAVETDAKLVAGQRKTGAAVIYGDA
SRPELLRKLHLDRALAVVLTMDHTAAAVHAVKGIRQSNPHVRIIARARDEKHALTLREAGASVVVPETLESGLKLTGSVL
ETLGVPADAASRLLEQERDRRIVAFRES

Sequences:

>Translated_588_residues
MHHFSASRYRMDHEAFPFLKETILFLALSGVLIPLLARLRINPVLGFLAVGTALGPYGLASFVPAHPWLAYVTFPRQEEV
ETLAELGVIFLMFMIGLELSVDRLWSLRRQVFGLGTLQVALTAALIGGIAHLFGNTTEASVILGLVLSFSSTAIVMQLLI
QRGELGGPLGQAAFAILLLQDLAVVPLLVLLSILGAGNSEGSFALLLGTAAAKGLVTVGLIYLIGRRLVRPVFHQITVSR
QPDTFMALTLLTTLGVAAMTWAAGLSMALGALLAGLIIAETEFRHEVEVTIEPFKGLLMGLFFLSVGMGIDLRALLAEPL
WIPLAVAGLMIIKGAVVFALLRLFGLSWGRAVEGGFLLGQGGEFAFIVIGLALSFKLLPRDVGQFMLLAVGLSMLATPVV
ARLGRELGAAMDRRQPPAAQEDAEFGTLRGHVLIAGYGRVGQMVGQMLAEQGVPHVAVETDAKLVAGQRKTGAAVIYGDA
SRPELLRKLHLDRALAVVLTMDHTAAAVHAVKGIRQSNPHVRIIARARDEKHALTLREAGASVVVPETLESGLKLTGSVL
ETLGVPADAASRLLEQERDRRIVAFRES
>Mature_588_residues
MHHFSASRYRMDHEAFPFLKETILFLALSGVLIPLLARLRINPVLGFLAVGTALGPYGLASFVPAHPWLAYVTFPRQEEV
ETLAELGVIFLMFMIGLELSVDRLWSLRRQVFGLGTLQVALTAALIGGIAHLFGNTTEASVILGLVLSFSSTAIVMQLLI
QRGELGGPLGQAAFAILLLQDLAVVPLLVLLSILGAGNSEGSFALLLGTAAAKGLVTVGLIYLIGRRLVRPVFHQITVSR
QPDTFMALTLLTTLGVAAMTWAAGLSMALGALLAGLIIAETEFRHEVEVTIEPFKGLLMGLFFLSVGMGIDLRALLAEPL
WIPLAVAGLMIIKGAVVFALLRLFGLSWGRAVEGGFLLGQGGEFAFIVIGLALSFKLLPRDVGQFMLLAVGLSMLATPVV
ARLGRELGAAMDRRQPPAAQEDAEFGTLRGHVLIAGYGRVGQMVGQMLAEQGVPHVAVETDAKLVAGQRKTGAAVIYGDA
SRPELLRKLHLDRALAVVLTMDHTAAAVHAVKGIRQSNPHVRIIARARDEKHALTLREAGASVVVPETLESGLKLTGSVL
ETLGVPADAASRLLEQERDRRIVAFRES

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=331, Percent_Identity=26.2839879154079, Blast_Score=66, Evalue=1e-10,
Organism=Escherichia coli, GI1786232, Length=558, Percent_Identity=36.3799283154122, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1789749, Length=564, Percent_Identity=38.2978723404255, Blast_Score=304, Evalue=8e-84,
Organism=Escherichia coli, GI1786685, Length=546, Percent_Identity=30.4029304029304, Blast_Score=185, Evalue=7e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 62802; Mature: 62802

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHHFSASRYRMDHEAFPFLKETILFLALSGVLIPLLARLRINPVLGFLAVGTALGPYGLA
CCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
SFVPAHPWLAYVTFPRQEEVETLAELGVIFLMFMIGLELSVDRLWSLRRQVFGLGTLQVA
HHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHH
LTAALIGGIAHLFGNTTEASVILGLVLSFSSTAIVMQLLIQRGELGGPLGQAAFAILLLQ
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
DLAVVPLLVLLSILGAGNSEGSFALLLGTAAAKGLVTVGLIYLIGRRLVRPVFHQITVSR
HHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QPDTFMALTLLTTLGVAAMTWAAGLSMALGALLAGLIIAETEFRHEVEVTIEPFKGLLMG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHH
LFFLSVGMGIDLRALLAEPLWIPLAVAGLMIIKGAVVFALLRLFGLSWGRAVEGGFLLGQ
HHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEC
GGEFAFIVIGLALSFKLLPRDVGQFMLLAVGLSMLATPVVARLGRELGAAMDRRQPPAAQ
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
EDAEFGTLRGHVLIAGYGRVGQMVGQMLAEQGVPHVAVETDAKLVAGQRKTGAAVIYGDA
CCCCCCEEECEEEEEECCHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCCCCCEEEECCC
SRPELLRKLHLDRALAVVLTMDHTAAAVHAVKGIRQSNPHVRIIARARDEKHALTLREAG
CCHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCC
ASVVVPETLESGLKLTGSVLETLGVPADAASRLLEQERDRRIVAFRES
CCEEEHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEECC
>Mature Secondary Structure
MHHFSASRYRMDHEAFPFLKETILFLALSGVLIPLLARLRINPVLGFLAVGTALGPYGLA
CCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
SFVPAHPWLAYVTFPRQEEVETLAELGVIFLMFMIGLELSVDRLWSLRRQVFGLGTLQVA
HHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHH
LTAALIGGIAHLFGNTTEASVILGLVLSFSSTAIVMQLLIQRGELGGPLGQAAFAILLLQ
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
DLAVVPLLVLLSILGAGNSEGSFALLLGTAAAKGLVTVGLIYLIGRRLVRPVFHQITVSR
HHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QPDTFMALTLLTTLGVAAMTWAAGLSMALGALLAGLIIAETEFRHEVEVTIEPFKGLLMG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHH
LFFLSVGMGIDLRALLAEPLWIPLAVAGLMIIKGAVVFALLRLFGLSWGRAVEGGFLLGQ
HHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEC
GGEFAFIVIGLALSFKLLPRDVGQFMLLAVGLSMLATPVVARLGRELGAAMDRRQPPAAQ
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
EDAEFGTLRGHVLIAGYGRVGQMVGQMLAEQGVPHVAVETDAKLVAGQRKTGAAVIYGDA
CCCCCCEEECEEEEEECCHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCCCCCEEEECCC
SRPELLRKLHLDRALAVVLTMDHTAAAVHAVKGIRQSNPHVRIIARARDEKHALTLREAG
CCHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCC
ASVVVPETLESGLKLTGSVLETLGVPADAASRLLEQERDRRIVAFRES
CCEEEHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA