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The map label for this gene is cysB [H]

Identifier: 119896725

GI number: 119896725

Start: 459444

End: 460385

Strand: Direct

Name: cysB [H]

Synonym: azo0434

Alternate gene names: 119896725

Gene position: 459444-460385 (Clockwise)

Preceding gene: 119896716

Following gene: 119896726

Centisome position: 10.5

GC content: 66.99

Gene sequence:

>942_bases
ATGAACCTGCAGCAACTGAGATACATCCAGGAAGTGGCCCGCCGCGGACTCAATGTCTCTGACGCCGCCGATGCGCTGTA
TACGTCCCAGCCGGGGGTGTCCAAGCAGATCCGCCTGCTCGAGGCGGAACTTGGCATCGAGATCTTCGCCCGCCACGGCA
AGCGCCTGGTGGCGGTCACCGAGCCGGGCCGCGCGGTGCTCGCGATCGCCGAACGCATGCTGCGCGACATGGACAACCTG
CTGCAGGTGGGCGACGAGTTCTCCAACGAAGCGGCCGGCGGGCTCTCGATCGCCACCACCCACACCCAGGCGCGTTACGC
GCTGCCGCCGGTGGTGCGCGAATTCATGCGGCGTTACCCGCAGGTCAAGCTGTCGCTGCACCAAGGCAGCCCGCACCAGG
TGTGCGAGATGGTGCTGGACGGCTCGGCGGACATCGCGATCGCCACCGAGGCAATTGCCGAATACGACGAGCTGGTGATG
CTGCCCTGCCACCAGTGGAACCGCTGCGTGGTCGCGTCGCCGCGCCATCCCATCCTGCGCGAGCAACCGCTGACGCTGGA
GGCGATCGCGCGCTATCCGCTGATCACCTACGACGACGCCTTCACCGGCCGCAGCCAGATCAACAAGGCCTTTCTCGGCC
GCGGCCTGAAGCCCAACGTGGTGCTCACCGCGCTCGACTCCGACGTGATCAAGACCTATGTGGCGATGGACCTTGGTATC
GGCATCCTCGCCCGCATGGCCTACGACCCGGAGGAAGACCGCAAGCTCGGCATGATCGACGCCGCCCACCTGTTCGAATC
GAGCACCACCCGTATCGGCCTGCGCCGCCGGGCGTGGCTGCGCGGTTATGTGTACGCCTTCGTCGAACTGTTTGCGCCGC
ACCTGACCCGCCGCATGGTGGAGACCGCGCTGGCGGGCGGCGGCAGCGATCCGGGACTCTAG

Upstream 100 bases:

>100_bases
GGCTTCCTTGATGTTGCGGCGCATCGTAGACCGACCTGTTTAGTTCAAAAAGAGCCTATGCGTTAGACTTTAAGAAGAAT
TGGTTATTAAGGTTTGTGAG

Downstream 100 bases:

>100_bases
GTCTTCGGACAGATGTCGGAAAATTTCACAGTACTAGTCCGAAAAGGCTAAGCCATTTGACATAGGTGTAGATTTTTCAA
AGCATTACCGGAGCGGGCTT

Product: transcriptional regulator CysB-like protein

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNLQQLRYIQEVARRGLNVSDAADALYTSQPGVSKQIRLLEAELGIEIFARHGKRLVAVTEPGRAVLAIAERMLRDMDNL
LQVGDEFSNEAAGGLSIATTHTQARYALPPVVREFMRRYPQVKLSLHQGSPHQVCEMVLDGSADIAIATEAIAEYDELVM
LPCHQWNRCVVASPRHPILREQPLTLEAIARYPLITYDDAFTGRSQINKAFLGRGLKPNVVLTALDSDVIKTYVAMDLGI
GILARMAYDPEEDRKLGMIDAAHLFESSTTRIGLRRRAWLRGYVYAFVELFAPHLTRRMVETALAGGGSDPGL

Sequences:

>Translated_313_residues
MNLQQLRYIQEVARRGLNVSDAADALYTSQPGVSKQIRLLEAELGIEIFARHGKRLVAVTEPGRAVLAIAERMLRDMDNL
LQVGDEFSNEAAGGLSIATTHTQARYALPPVVREFMRRYPQVKLSLHQGSPHQVCEMVLDGSADIAIATEAIAEYDELVM
LPCHQWNRCVVASPRHPILREQPLTLEAIARYPLITYDDAFTGRSQINKAFLGRGLKPNVVLTALDSDVIKTYVAMDLGI
GILARMAYDPEEDRKLGMIDAAHLFESSTTRIGLRRRAWLRGYVYAFVELFAPHLTRRMVETALAGGGSDPGL
>Mature_313_residues
MNLQQLRYIQEVARRGLNVSDAADALYTSQPGVSKQIRLLEAELGIEIFARHGKRLVAVTEPGRAVLAIAERMLRDMDNL
LQVGDEFSNEAAGGLSIATTHTQARYALPPVVREFMRRYPQVKLSLHQGSPHQVCEMVLDGSADIAIATEAIAEYDELVM
LPCHQWNRCVVASPRHPILREQPLTLEAIARYPLITYDDAFTGRSQINKAFLGRGLKPNVVLTALDSDVIKTYVAMDLGI
GILARMAYDPEEDRKLGMIDAAHLFESSTTRIGLRRRAWLRGYVYAFVELFAPHLTRRMVETALAGGGSDPGL

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=305, Percent_Identity=53.4426229508197, Blast_Score=343, Evalue=7e-96,
Organism=Escherichia coli, GI1788296, Length=290, Percent_Identity=48.2758620689655, Blast_Score=287, Evalue=8e-79,
Organism=Escherichia coli, GI157672245, Length=221, Percent_Identity=29.4117647058824, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788481, Length=245, Percent_Identity=30.6122448979592, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790399, Length=254, Percent_Identity=24.8031496062992, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI2367136, Length=243, Percent_Identity=25.1028806584362, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788887, Length=249, Percent_Identity=28.9156626506024, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787806, Length=171, Percent_Identity=28.6549707602339, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787601, Length=180, Percent_Identity=26.6666666666667, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788748, Length=254, Percent_Identity=24.8031496062992, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34767; Mature: 34767

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLQQLRYIQEVARRGLNVSDAADALYTSQPGVSKQIRLLEAELGIEIFARHGKRLVAVT
CCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEE
EPGRAVLAIAERMLRDMDNLLQVGDEFSNEAAGGLSIATTHTQARYALPPVVREFMRRYP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHCCCHHHHHHHHHCC
QVKLSLHQGSPHQVCEMVLDGSADIAIATEAIAEYDELVMLPCHQWNRCVVASPRHPILR
CEEEEECCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHEEEECCCCCCEEEEECCCCCCCC
EQPLTLEAIARYPLITYDDAFTGRSQINKAFLGRGLKPNVVLTALDSDVIKTYVAMDLGI
CCCCCHHHHHCCCEEEECCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHH
GILARMAYDPEEDRKLGMIDAAHLFESSTTRIGLRRRAWLRGYVYAFVELFAPHLTRRMV
HHHHHHCCCCCCCCEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETALAGGGSDPGL
HHHHHCCCCCCCC
>Mature Secondary Structure
MNLQQLRYIQEVARRGLNVSDAADALYTSQPGVSKQIRLLEAELGIEIFARHGKRLVAVT
CCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEE
EPGRAVLAIAERMLRDMDNLLQVGDEFSNEAAGGLSIATTHTQARYALPPVVREFMRRYP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHCCCHHHHHHHHHCC
QVKLSLHQGSPHQVCEMVLDGSADIAIATEAIAEYDELVMLPCHQWNRCVVASPRHPILR
CEEEEECCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHEEEECCCCCCEEEEECCCCCCCC
EQPLTLEAIARYPLITYDDAFTGRSQINKAFLGRGLKPNVVLTALDSDVIKTYVAMDLGI
CCCCCHHHHHCCCEEEECCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHH
GILARMAYDPEEDRKLGMIDAAHLFESSTTRIGLRRRAWLRGYVYAFVELFAPHLTRRMV
HHHHHHCCCCCCCCEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETALAGGGSDPGL
HHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]