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The map label for this gene is hslU

Identifier: 119896715

GI number: 119896715

Start: 447833

End: 449164

Strand: Direct

Name: hslU

Synonym: azo0424

Alternate gene names: 119896715

Gene position: 447833-449164 (Clockwise)

Preceding gene: 119896714

Following gene: 119896716

Centisome position: 10.23

GC content: 66.14

Gene sequence:

>1332_bases
ATGACCCAGATGACCCCGCCGGAGATCGTCTCCGAACTCGACAAGCACATCGTCGGCCAGGCCCGCGCCAAGAAGGCCGT
GGCGATCGCGCTGCGCAACCGCTGGCGCCGTGCGCGCATCGACGAACCGCTGCGCAGCGAGATCACGCCCAAGAACATCC
TGATGATCGGCCCCACCGGGGTCGGCAAGACCGAGATTGCGCGCCGCCTGGCACGGCTCGCCAACGCGCCCTTCATCAAG
GTCGAGGCCACCAAGTTCACCGAAGTGGGTTACGTCGGGCGCGACGTGGACACCATCATCCGAGACCTCGTCGAAATCGC
GATCAAGGATGGCCGCGAGCGGGCGATGCGTGTGGTGCGCGACCGTGCCCTCGACGCTGCCGAGGACCGCGTGCTCGATG
TGCTGCTGCCGCCGGCCCGCCCGGTCGGGTTCTCCGAACCGGCCCAGCCGCAGGATTCCGCCACGCGGCAGAAATTCCGC
AAGAAGCTGCGCGAAGGCGAGCTGGACGACAAGGAAGTGGAGATCGAGGTCGCAAGTGCCCCGATGCAGGCCGAAATCTT
CGCCCCGCCCGGCATGGAAGAGCTGACGCAGCAAATCCAGGGCATGTTCCAGAACCTGGGCAACAGCCGCAAGAAACTGC
GCAAGCTGCCGATCCGCGAGGCGCTCAAGCTGCTGGCCGATGAGGAAGCGGCGCGCCTGATCAACGACGAGGAAGTGAAG
ACCGAAGCGTTGCGGGCGGTGGAGCAGAACGGCATCGTCTTTCTCGACGAGGTCGACAAGATCGCCGCCCGGGCCGACGC
CCACGGCGCCGACGTGTCGCGCCAGGGCGTGCAGCGCGACCTGCTGCCGCTGGTGGAAGGGACCACGATCTCGACCAAGT
ACGGCATGATCAAGACCGATCACATCCTGTTCATCGCCAGTGGCGCCTTCCATCTCTCCAAGCCGAGCGACCTGATCCCG
GAGCTGCAGGGGCGCTTCCCGATCCGCGTGGAGCTGGAGTCGCTGTCGGTGGAAGACTTCGAGCGCATCCTGACCAGCAC
CGATGCCTGCCTGACGCGCCAGTACGAGGCCCTGCTGGCCACCGACGGCGTGACCCTGAGCTTCACGCCCGAGGGCATCC
GGCGGCTGGCCGAGATTGCTTACCAGGTCAACGAGAAGACCGAGAACATCGGCGCGCGCCGGCTCTACACGGTGATGGAG
AAACTGCTCGAAGAGGTCTCTTTCGAGGCCGGCAAGGTCGGCGTCGACAAGCTCACGGTCGATGCGGCCTACGTGGACGC
CCGTCTCGAAGTCCTCGCGCAGCGCGAGGATCTCGCGCGCTACGTGCTCTGA

Upstream 100 bases:

>100_bases
CTCGCCATCGCCGGCGACCTCTGCATCTACACCAACCAGTGCCACACGATCGAAGTGCTGGACTGAGCGCGCCGTCGTAA
CCCGTTTCGAGCGAACCGCC

Downstream 100 bases:

>100_bases
GTTTCGCCCGCCTTTCCGGCCCTTCTTCCCGTGCCCGCCCCGGCCCGTAGCGGCGTGATCCGTTGCGGGGCCGGGGCGCG
GCCGCCATTCATGATGTCTT

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MTQMTPPEIVSELDKHIVGQARAKKAVAIALRNRWRRARIDEPLRSEITPKNILMIGPTGVGKTEIARRLARLANAPFIK
VEATKFTEVGYVGRDVDTIIRDLVEIAIKDGRERAMRVVRDRALDAAEDRVLDVLLPPARPVGFSEPAQPQDSATRQKFR
KKLREGELDDKEVEIEVASAPMQAEIFAPPGMEELTQQIQGMFQNLGNSRKKLRKLPIREALKLLADEEAARLINDEEVK
TEALRAVEQNGIVFLDEVDKIAARADAHGADVSRQGVQRDLLPLVEGTTISTKYGMIKTDHILFIASGAFHLSKPSDLIP
ELQGRFPIRVELESLSVEDFERILTSTDACLTRQYEALLATDGVTLSFTPEGIRRLAEIAYQVNEKTENIGARRLYTVME
KLLEEVSFEAGKVGVDKLTVDAAYVDARLEVLAQREDLARYVL

Sequences:

>Translated_443_residues
MTQMTPPEIVSELDKHIVGQARAKKAVAIALRNRWRRARIDEPLRSEITPKNILMIGPTGVGKTEIARRLARLANAPFIK
VEATKFTEVGYVGRDVDTIIRDLVEIAIKDGRERAMRVVRDRALDAAEDRVLDVLLPPARPVGFSEPAQPQDSATRQKFR
KKLREGELDDKEVEIEVASAPMQAEIFAPPGMEELTQQIQGMFQNLGNSRKKLRKLPIREALKLLADEEAARLINDEEVK
TEALRAVEQNGIVFLDEVDKIAARADAHGADVSRQGVQRDLLPLVEGTTISTKYGMIKTDHILFIASGAFHLSKPSDLIP
ELQGRFPIRVELESLSVEDFERILTSTDACLTRQYEALLATDGVTLSFTPEGIRRLAEIAYQVNEKTENIGARRLYTVME
KLLEEVSFEAGKVGVDKLTVDAAYVDARLEVLAQREDLARYVL
>Mature_442_residues
TQMTPPEIVSELDKHIVGQARAKKAVAIALRNRWRRARIDEPLRSEITPKNILMIGPTGVGKTEIARRLARLANAPFIKV
EATKFTEVGYVGRDVDTIIRDLVEIAIKDGRERAMRVVRDRALDAAEDRVLDVLLPPARPVGFSEPAQPQDSATRQKFRK
KLREGELDDKEVEIEVASAPMQAEIFAPPGMEELTQQIQGMFQNLGNSRKKLRKLPIREALKLLADEEAARLINDEEVKT
EALRAVEQNGIVFLDEVDKIAARADAHGADVSRQGVQRDLLPLVEGTTISTKYGMIKTDHILFIASGAFHLSKPSDLIPE
LQGRFPIRVELESLSVEDFERILTSTDACLTRQYEALLATDGVTLSFTPEGIRRLAEIAYQVNEKTENIGARRLYTVMEK
LLEEVSFEAGKVGVDKLTVDAAYVDARLEVLAQREDLARYVL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily

Homologues:

Organism=Escherichia coli, GI1790366, Length=443, Percent_Identity=65.2370203160271, Blast_Score=604, Evalue=1e-174,
Organism=Escherichia coli, GI1786642, Length=102, Percent_Identity=48.0392156862745, Blast_Score=98, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI71982908, Length=223, Percent_Identity=35.4260089686099, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71982905, Length=223, Percent_Identity=34.0807174887892, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319704, Length=141, Percent_Identity=33.3333333333333, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648291, Length=108, Percent_Identity=37.037037037037, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648289, Length=108, Percent_Identity=37.037037037037, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSLU_AZOSB (A1K2I6)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_931928.1
- ProteinModelPortal:   A1K2I6
- SMR:   A1K2I6
- STRING:   A1K2I6
- GeneID:   4609037
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo0424
- eggNOG:   COG1220
- HOGENOM:   HBG745965
- OMA:   EASKFTE
- PhylomeDB:   A1K2I6
- ProtClustDB:   PRK05201
- BioCyc:   ASP62928:AZO0424-MONOMER
- HAMAP:   MF_00249
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491
- PANTHER:   PTHR11262:SF3
- SMART:   SM00382
- TIGRFAMs:   TIGR00390

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small

EC number: NA

Molecular weight: Translated: 49538; Mature: 49406

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: BINDING 18-18 BINDING 256-256 BINDING 321-321 BINDING 393-393

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQMTPPEIVSELDKHIVGQARAKKAVAIALRNRWRRARIDEPLRSEITPKNILMIGPTG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCEEEECCCC
VGKTEIARRLARLANAPFIKVEATKFTEVGYVGRDVDTIIRDLVEIAIKDGRERAMRVVR
CCHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
DRALDAAEDRVLDVLLPPARPVGFSEPAQPQDSATRQKFRKKLREGELDDKEVEIEVASA
HHHCCHHHHHEEHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECC
PMQAEIFAPPGMEELTQQIQGMFQNLGNSRKKLRKLPIREALKLLADEEAARLINDEEVK
CCEEEEECCCCHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHCHHHHHHCCHHHHH
TEALRAVEQNGIVFLDEVDKIAARADAHGADVSRQGVQRDLLPLVEGTTISTKYGMIKTD
HHHHHHHHHCCEEEEECHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCEEEC
HILFIASGAFHLSKPSDLIPELQGRFPIRVELESLSVEDFERILTSTDACLTRQYEALLA
CEEEEEECCCCCCCCHHHHHHHCCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHE
TDGVTLSFTPEGIRRLAEIAYQVNEKTENIGARRLYTVMEKLLEEVSFEAGKVGVDKLTV
ECCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEE
DAAYVDARLEVLAQREDLARYVL
HHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQMTPPEIVSELDKHIVGQARAKKAVAIALRNRWRRARIDEPLRSEITPKNILMIGPTG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCEEEECCCC
VGKTEIARRLARLANAPFIKVEATKFTEVGYVGRDVDTIIRDLVEIAIKDGRERAMRVVR
CCHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
DRALDAAEDRVLDVLLPPARPVGFSEPAQPQDSATRQKFRKKLREGELDDKEVEIEVASA
HHHCCHHHHHEEHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECC
PMQAEIFAPPGMEELTQQIQGMFQNLGNSRKKLRKLPIREALKLLADEEAARLINDEEVK
CCEEEEECCCCHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHCHHHHHHCCHHHHH
TEALRAVEQNGIVFLDEVDKIAARADAHGADVSRQGVQRDLLPLVEGTTISTKYGMIKTD
HHHHHHHHHCCEEEEECHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCEEEC
HILFIASGAFHLSKPSDLIPELQGRFPIRVELESLSVEDFERILTSTDACLTRQYEALLA
CEEEEEECCCCCCCCHHHHHHHCCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHE
TDGVTLSFTPEGIRRLAEIAYQVNEKTENIGARRLYTVMEKLLEEVSFEAGKVGVDKLTV
ECCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEE
DAAYVDARLEVLAQREDLARYVL
HHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA