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The map label for this gene is pcrA [H]

Identifier: 119896636

GI number: 119896636

Start: 367763

End: 369865

Strand: Reverse

Name: pcrA [H]

Synonym: azo0345

Alternate gene names: 119896636

Gene position: 369865-367763 (Counterclockwise)

Preceding gene: 119896641

Following gene: 119896633

Centisome position: 8.45

GC content: 69.61

Gene sequence:

>2103_bases
ATGAACAGCCCCGCTCCTGCCGCCTGCGCGCCGGCCCCCGCCTATCTCGACCGTCTCAATGCCGCGCAGCGCGAGGCGGT
GGAATATGGTGTGCGCGGCAACGCCGGGCGCGACATCCCCGGCCCGCTGCTGGTCATCGCCGGCGCCGGCTCGGGCAAGA
CCAACACGCTCGCGCACCGTGTCGCCCACCTGATCGCCAACGGCGCCGATCCCGGCCGCATCCTGCTGCTCACCTTCTCG
CGCCGCGCGGCGGACGAAATGGGCCGGCGGGTGCAGCGCATCCTCGCCCAGGTGGCGGTCGATACCCCCTGGCTGGCGCA
GGCCACGCTGCAGTGGTCGGGCACCTTCCACGGCATCGGCGCGCGGCTGCTGCGCGAATACGCCGGCCGCATCGGCCTGG
ACCCGGCCTTCACCATCCACGACCGCGAGGATTCCGCCGACCTGATGAACCTCGCGCGCCACGAGGCCGGGCTGTCCACC
CGCAACAAGCGCTTTCCGCTCAAAGGCACCTGCCTGGCGATCTACTCGGCGGCGGTGAACACGCGCGCGCCGCTGGCCGA
GGTGCTGCAGACCACCTTCCCCTGGTGCGCCGAATGGGAAGCCGAGCTGAAGACGCTGTTCCGCGCCTATGTGCAGGCCA
AGCAGGCGCAGCAGGTGCTGGATTACGACGACCTGCTGCTGTACTGGGCGCACATGGTGGCGGAGCCGGCGCTGGGGGCG
GAGATCGGCGGCCTCTTCCAGCATGTGCTGGTCGACGAATACCAGGACACCAACCACCTGCAGGCCGAGATCCTGCTGGC
GATGAAGCCGGACGGCCGCGGCCTCACCGTGGTGGGCGACGACGCCCAGTCGATCTACGCCTTCCGCGGCGCCACCATCC
GCAACATCCTGGATTTCCCCGCCCGCTTCGAACCACCCGCGCACCGGGTGACGCTGGAGCAGAACTACCGCTCGACGCGG
CCCATCCTCGACGCCGCCAACGCGGTGATCGCGCAGGCCGCCGAGCGCTACAGCAAGGACCTGTGGTCGCTGCGCGAATC
CACCGCGCGCCCTCAATTGGTGTCGGTGCGCGACGACGCCGACCAGTCCGCCTTCGTGGTGGAACGCGTGCTGGCCCGCC
GCGAGCAGGGCATCAAGCTCAAGCAGCAGGCGGTGCTGTTCCGCGCCGCCAGCCACAGCGCGCGGCTGGAGATGGAGCTG
ACCCGGCGCAACATCCCGTTCGTGAAGTTCGGCGGGCTCAAGTTCCTCGAGGCCGCGCATGTGAAGGACATGCTGGCGGT
ACTGCGCTGGGTGGAAAACCTGCGCGACCGGGTGTCCGGCTTCCGCGTGGTGCAACTGCTCGTCGGCATTGGCCCCAAGA
CCGCCGGCAAGGTGCTGGACCACGTGGTCGCGGCGCCCGAAGGCTGCGAACTGCTCGCCGAGCAGCCGGTGCCGGAAGCC
GCGCGCGCCGACTGGCAGGCATTTTCCACGCTGATCGAACACCTGCGCCGGCCCGACGCGCCGTGGCCGGCGAGCTTCGA
ACTCGCCTGCCGCTGGTACGAGGGCCAGATGCCGCGGCTATACGACGACGCGGTGGTGCGCCAGGGCGACATCCAGCAGC
TCGGCCGCATCGCCGCCACCTACCCGAGCCGCCAGCGCTTCCTCACCGAACTCACGCTCGATCCACCCAGCGCCACCAGC
GACGAGGCCGGCGTGCCGCTGCTCGACGAGGACTATCTGATCCTGTCGACCATGCATTCGGCCAAGGGCCAGGAATGGAA
TTCGGTGAGCGTGCTCAACGTGGTGGACGGCTGCGTGCCCTCCGACATCGCCACCCGCAGCAGCGCGGAAATCGAGGAAG
AACGCCGCCTGCTCTACGTGGCGATGACGCGCGCCAAGGACCATCTGGAGCTGCTGGTGCCGCAGCGCTTCTACGTGTCG
CAGCAGCAGGGCATGGGCGACCGCCATGTGTACGCCGGCCGCACCCGCTTCATCCCCAACCGGCTGCTGCCGCAGTTCGA
ACAGGTGTCGTGGCCGGAGCCGCCGCCGGAATTCCGCGGCACGCCACAGTCGCGTCAGGCCGCGCTGGATCTGGCGGCGA
AGATGCGCGGCATGTGGGATTAA

Upstream 100 bases:

>100_bases
TAGACCCCGCTCTCCCCGGACGGGCGCCAGTTTAGCGCCGCCGCCCGCCGCCTGCCGCGCGGCTACCGTAGGCGGGGGTG
GACTGGATAGAATTACAGGC

Downstream 100 bases:

>100_bases
GGGCGAAGCGACGGCCGGGCTGAGCCGCGCCCCACCCCGTTCGGGCCCGGACACCCCGACCACCGTCGGGGCTGAGCGTG
TCGAAGCCCGCTTGCCGCGC

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 700; Mature: 700

Protein sequence:

>700_residues
MNSPAPAACAPAPAYLDRLNAAQREAVEYGVRGNAGRDIPGPLLVIAGAGSGKTNTLAHRVAHLIANGADPGRILLLTFS
RRAADEMGRRVQRILAQVAVDTPWLAQATLQWSGTFHGIGARLLREYAGRIGLDPAFTIHDREDSADLMNLARHEAGLST
RNKRFPLKGTCLAIYSAAVNTRAPLAEVLQTTFPWCAEWEAELKTLFRAYVQAKQAQQVLDYDDLLLYWAHMVAEPALGA
EIGGLFQHVLVDEYQDTNHLQAEILLAMKPDGRGLTVVGDDAQSIYAFRGATIRNILDFPARFEPPAHRVTLEQNYRSTR
PILDAANAVIAQAAERYSKDLWSLRESTARPQLVSVRDDADQSAFVVERVLARREQGIKLKQQAVLFRAASHSARLEMEL
TRRNIPFVKFGGLKFLEAAHVKDMLAVLRWVENLRDRVSGFRVVQLLVGIGPKTAGKVLDHVVAAPEGCELLAEQPVPEA
ARADWQAFSTLIEHLRRPDAPWPASFELACRWYEGQMPRLYDDAVVRQGDIQQLGRIAATYPSRQRFLTELTLDPPSATS
DEAGVPLLDEDYLILSTMHSAKGQEWNSVSVLNVVDGCVPSDIATRSSAEIEEERRLLYVAMTRAKDHLELLVPQRFYVS
QQQGMGDRHVYAGRTRFIPNRLLPQFEQVSWPEPPPEFRGTPQSRQAALDLAAKMRGMWD

Sequences:

>Translated_700_residues
MNSPAPAACAPAPAYLDRLNAAQREAVEYGVRGNAGRDIPGPLLVIAGAGSGKTNTLAHRVAHLIANGADPGRILLLTFS
RRAADEMGRRVQRILAQVAVDTPWLAQATLQWSGTFHGIGARLLREYAGRIGLDPAFTIHDREDSADLMNLARHEAGLST
RNKRFPLKGTCLAIYSAAVNTRAPLAEVLQTTFPWCAEWEAELKTLFRAYVQAKQAQQVLDYDDLLLYWAHMVAEPALGA
EIGGLFQHVLVDEYQDTNHLQAEILLAMKPDGRGLTVVGDDAQSIYAFRGATIRNILDFPARFEPPAHRVTLEQNYRSTR
PILDAANAVIAQAAERYSKDLWSLRESTARPQLVSVRDDADQSAFVVERVLARREQGIKLKQQAVLFRAASHSARLEMEL
TRRNIPFVKFGGLKFLEAAHVKDMLAVLRWVENLRDRVSGFRVVQLLVGIGPKTAGKVLDHVVAAPEGCELLAEQPVPEA
ARADWQAFSTLIEHLRRPDAPWPASFELACRWYEGQMPRLYDDAVVRQGDIQQLGRIAATYPSRQRFLTELTLDPPSATS
DEAGVPLLDEDYLILSTMHSAKGQEWNSVSVLNVVDGCVPSDIATRSSAEIEEERRLLYVAMTRAKDHLELLVPQRFYVS
QQQGMGDRHVYAGRTRFIPNRLLPQFEQVSWPEPPPEFRGTPQSRQAALDLAAKMRGMWD
>Mature_700_residues
MNSPAPAACAPAPAYLDRLNAAQREAVEYGVRGNAGRDIPGPLLVIAGAGSGKTNTLAHRVAHLIANGADPGRILLLTFS
RRAADEMGRRVQRILAQVAVDTPWLAQATLQWSGTFHGIGARLLREYAGRIGLDPAFTIHDREDSADLMNLARHEAGLST
RNKRFPLKGTCLAIYSAAVNTRAPLAEVLQTTFPWCAEWEAELKTLFRAYVQAKQAQQVLDYDDLLLYWAHMVAEPALGA
EIGGLFQHVLVDEYQDTNHLQAEILLAMKPDGRGLTVVGDDAQSIYAFRGATIRNILDFPARFEPPAHRVTLEQNYRSTR
PILDAANAVIAQAAERYSKDLWSLRESTARPQLVSVRDDADQSAFVVERVLARREQGIKLKQQAVLFRAASHSARLEMEL
TRRNIPFVKFGGLKFLEAAHVKDMLAVLRWVENLRDRVSGFRVVQLLVGIGPKTAGKVLDHVVAAPEGCELLAEQPVPEA
ARADWQAFSTLIEHLRRPDAPWPASFELACRWYEGQMPRLYDDAVVRQGDIQQLGRIAATYPSRQRFLTELTLDPPSATS
DEAGVPLLDEDYLILSTMHSAKGQEWNSVSVLNVVDGCVPSDIATRSSAEIEEERRLLYVAMTRAKDHLELLVPQRFYVS
QQQGMGDRHVYAGRTRFIPNRLLPQFEQVSWPEPPPEFRGTPQSRQAALDLAAKMRGMWD

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=671, Percent_Identity=32.0417287630402, Blast_Score=248, Evalue=8e-67,
Organism=Escherichia coli, GI48994965, Length=449, Percent_Identity=31.6258351893096, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6322369, Length=529, Percent_Identity=26.0869565217391, Blast_Score=130, Evalue=8e-31,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77900; Mature: 77900

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSPAPAACAPAPAYLDRLNAAQREAVEYGVRGNAGRDIPGPLLVIAGAGSGKTNTLAHR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHH
VAHLIANGADPGRILLLTFSRRAADEMGRRVQRILAQVAVDTPWLAQATLQWSGTFHGIG
HHHHHHCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEECCCHHHHH
ARLLREYAGRIGLDPAFTIHDREDSADLMNLARHEAGLSTRNKRFPLKGTCLAIYSAAVN
HHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHC
TRAPLAEVLQTTFPWCAEWEAELKTLFRAYVQAKQAQQVLDYDDLLLYWAHMVAEPALGA
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCH
EIGGLFQHVLVDEYQDTNHLQAEILLAMKPDGRGLTVVGDDAQSIYAFRGATIRNILDFP
HHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEECCCHHHHHHHCCCHHHHHHHCC
ARFEPPAHRVTLEQNYRSTRPILDAANAVIAQAAERYSKDLWSLRESTARPQLVSVRDDA
CCCCCCCCEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
DQSAFVVERVLARREQGIKLKQQAVLFRAASHSARLEMELTRRNIPFVKFGGLKFLEAAH
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEEHHCCCCEEEECCHHHHHHHH
VKDMLAVLRWVENLRDRVSGFRVVQLLVGIGPKTAGKVLDHVVAAPEGCELLAEQPVPEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHCCCCCHH
ARADWQAFSTLIEHLRRPDAPWPASFELACRWYEGQMPRLYDDAVVRQGDIQQLGRIAAT
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHC
YPSRQRFLTELTLDPPSATSDEAGVPLLDEDYLILSTMHSAKGQEWNSVSVLNVVDGCVP
CCHHHHHHHHEECCCCCCCCCCCCCCCCCCCEEEEEEHHHHCCCCCCCEEHHHHHCCCCC
SDIATRSSAEIEEERRLLYVAMTRAKDHLELLVPQRFYVSQQQGMGDRHVYAGRTRFIPN
CHHHCCCCHHHHHHHHEEEEEEHHCHHHHHHHCCHHHHHHHHCCCCCCEEECCCCCCCCH
RLLPQFEQVSWPEPPPEFRGTPQSRQAALDLAAKMRGMWD
HHCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNSPAPAACAPAPAYLDRLNAAQREAVEYGVRGNAGRDIPGPLLVIAGAGSGKTNTLAHR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHH
VAHLIANGADPGRILLLTFSRRAADEMGRRVQRILAQVAVDTPWLAQATLQWSGTFHGIG
HHHHHHCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEECCCHHHHH
ARLLREYAGRIGLDPAFTIHDREDSADLMNLARHEAGLSTRNKRFPLKGTCLAIYSAAVN
HHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHC
TRAPLAEVLQTTFPWCAEWEAELKTLFRAYVQAKQAQQVLDYDDLLLYWAHMVAEPALGA
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCH
EIGGLFQHVLVDEYQDTNHLQAEILLAMKPDGRGLTVVGDDAQSIYAFRGATIRNILDFP
HHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEECCCHHHHHHHCCCHHHHHHHCC
ARFEPPAHRVTLEQNYRSTRPILDAANAVIAQAAERYSKDLWSLRESTARPQLVSVRDDA
CCCCCCCCEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
DQSAFVVERVLARREQGIKLKQQAVLFRAASHSARLEMELTRRNIPFVKFGGLKFLEAAH
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEEHHCCCCEEEECCHHHHHHHH
VKDMLAVLRWVENLRDRVSGFRVVQLLVGIGPKTAGKVLDHVVAAPEGCELLAEQPVPEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHCCCCCHH
ARADWQAFSTLIEHLRRPDAPWPASFELACRWYEGQMPRLYDDAVVRQGDIQQLGRIAAT
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHC
YPSRQRFLTELTLDPPSATSDEAGVPLLDEDYLILSTMHSAKGQEWNSVSVLNVVDGCVP
CCHHHHHHHHEECCCCCCCCCCCCCCCCCCCEEEEEEHHHHCCCCCCCEEHHHHHCCCCC
SDIATRSSAEIEEERRLLYVAMTRAKDHLELLVPQRFYVSQQQGMGDRHVYAGRTRFIPN
CHHHCCCCHHHHHHHHEEEEEEHHCHHHHHHHCCHHHHHHHHCCCCCCEEECCCCCCCCH
RLLPQFEQVSWPEPPPEFRGTPQSRQAALDLAAKMRGMWD
HHCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]