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The map label for this gene is ycgF [H]

Identifier: 119896626

GI number: 119896626

Start: 359461

End: 360240

Strand: Direct

Name: ycgF [H]

Synonym: azo0335

Alternate gene names: 119896626

Gene position: 359461-360240 (Clockwise)

Preceding gene: 119896624

Following gene: 119896627

Centisome position: 8.21

GC content: 70.13

Gene sequence:

>780_bases
ATGAGCGACCACCCGCCATGTGCCGGCTGCAGCGGCCAGCCGCTGGCGTTCGGATTCAGCTACGCCTACCAGCCGATCGT
CGATCTTGCGGCGCGCCAGATCTTTGCCCACGAGGCGCTGGTGCGCGGCCCGGCCGGCGAGGGCGCGGGCACCGTGCTGG
CGCAGGTCAATGCCGACAACCGCTACCGCTTCGACCAGGCCTGCCGGGTGCGGGCGATCGAAACCGCGGCGCGGCTGGGC
ATGCAGTCGCGGCTGTCGATCAACTTCCTGCCCAACGCCATCCAGCGTCCCGACCTGTGCATCCGTTCGACGCTGGAGGC
GGCGCGCCTTCACGGCTTTCCGCTGGAGCGCATCATCTTCGAGAGCGTGGAGGGCGAGCGCATCGCCGACGCGCGCTGGT
TGGCCGAGGTGTTCCGCGAATGGCAGCACCACGGCTTTCTGACCGCGATCGACGATTTCGGCGCCGGCTACGCCGGCCTC
AACCTGCTGGCCGATTTCCAGCCCGACCTGGTCAAGCTCGACATGGGGCTGGTGCGCGCGGTGCACCGTCATCCGGCGCG
CCAGGCCATCGTGCGCGGCGTCGCGCGGATCTGCGAGGAACTCGACATCCCGGTGGTGGCCGAGGGCGTGGAGGAACTCG
ATGAGGCGCGCTGCCTCAGCGACGCCGGCATCCGGTTGATGCAGGGCTTCCTGTTCTGCCGTCCCTTGTTCGAAGGCGTG
GGCGAGGCCGCCACGCTCGCGTGGCCCGCGCTTGCGCCGCAGGCCGGAGGACGGCAGTGA

Upstream 100 bases:

>100_bases
ATCCCTGCCCGCGCGGGTAGACTGGCGTGTCTTGCGCAAGCGCCCGTCGGCGGCCAGCCGACCCCGGCATCCTCAACCCT
GCGATCAGGAGCGCGCCACG

Downstream 100 bases:

>100_bases
GCAGCCAGCGCGAGAAACTGCTCGAAGCCGCGGTTGCACAGTCGTTCAACGCGGTCGTGATCACCGACGCCCGCCTGGAT
GGCGGCGGCCCCCACATCGT

Product: EAL-domain-containing protein

Products: NA

Alternate protein names: Blrp [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MSDHPPCAGCSGQPLAFGFSYAYQPIVDLAARQIFAHEALVRGPAGEGAGTVLAQVNADNRYRFDQACRVRAIETAARLG
MQSRLSINFLPNAIQRPDLCIRSTLEAARLHGFPLERIIFESVEGERIADARWLAEVFREWQHHGFLTAIDDFGAGYAGL
NLLADFQPDLVKLDMGLVRAVHRHPARQAIVRGVARICEELDIPVVAEGVEELDEARCLSDAGIRLMQGFLFCRPLFEGV
GEAATLAWPALAPQAGGRQ

Sequences:

>Translated_259_residues
MSDHPPCAGCSGQPLAFGFSYAYQPIVDLAARQIFAHEALVRGPAGEGAGTVLAQVNADNRYRFDQACRVRAIETAARLG
MQSRLSINFLPNAIQRPDLCIRSTLEAARLHGFPLERIIFESVEGERIADARWLAEVFREWQHHGFLTAIDDFGAGYAGL
NLLADFQPDLVKLDMGLVRAVHRHPARQAIVRGVARICEELDIPVVAEGVEELDEARCLSDAGIRLMQGFLFCRPLFEGV
GEAATLAWPALAPQAGGRQ
>Mature_258_residues
SDHPPCAGCSGQPLAFGFSYAYQPIVDLAARQIFAHEALVRGPAGEGAGTVLAQVNADNRYRFDQACRVRAIETAARLGM
QSRLSINFLPNAIQRPDLCIRSTLEAARLHGFPLERIIFESVEGERIADARWLAEVFREWQHHGFLTAIDDFGAGYAGLN
LLADFQPDLVKLDMGLVRAVHRHPARQAIVRGVARICEELDIPVVAEGVEELDEARCLSDAGIRLMQGFLFCRPLFEGVG
EAATLAWPALAPQAGGRQ

Specific function: Binds to and releases the YcgE repressor from its bound DNA target in a blue-light-dependent (470 nm) fashion. A shift to low temperature also triggers a YcgF-medisted relief of repression by YcgE, suggesting YcgF may serve as a thermometer. Blue light ma

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1787410, Length=231, Percent_Identity=34.1991341991342, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1787541, Length=173, Percent_Identity=31.7919075144509, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788502, Length=231, Percent_Identity=29.004329004329, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI226510982, Length=231, Percent_Identity=25.5411255411255, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87081980, Length=177, Percent_Identity=25.9887005649718, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1790496, Length=224, Percent_Identity=25, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI87082096, Length=215, Percent_Identity=25.5813953488372, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI87081845, Length=120, Percent_Identity=32.5, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787055, Length=227, Percent_Identity=29.5154185022026, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1786507, Length=120, Percent_Identity=27.5, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788849, Length=152, Percent_Identity=26.9736842105263, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR007024
- InterPro:   IPR001633 [H]

Pfam domain/function: PF04940 BLUF; PF00563 EAL [H]

EC number: NA

Molecular weight: Translated: 28346; Mature: 28215

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDHPPCAGCSGQPLAFGFSYAYQPIVDLAARQIFAHEALVRGPAGEGAGTVLAQVNADN
CCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCC
RYRFDQACRVRAIETAARLGMQSRLSINFLPNAIQRPDLCIRSTLEAARLHGFPLERIIF
CCCHHHHHHHHHHHHHHHHCHHHCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHH
ESVEGERIADARWLAEVFREWQHHGFLTAIDDFGAGYAGLNLLADFQPDLVKLDMGLVRA
HHCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHH
VHRHPARQAIVRGVARICEELDIPVVAEGVEELDEARCLSDAGIRLMQGFLFCRPLFEGV
HHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GEAATLAWPALAPQAGGRQ
CCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SDHPPCAGCSGQPLAFGFSYAYQPIVDLAARQIFAHEALVRGPAGEGAGTVLAQVNADN
CCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCC
RYRFDQACRVRAIETAARLGMQSRLSINFLPNAIQRPDLCIRSTLEAARLHGFPLERIIF
CCCHHHHHHHHHHHHHHHHCHHHCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHH
ESVEGERIADARWLAEVFREWQHHGFLTAIDDFGAGYAGLNLLADFQPDLVKLDMGLVRA
HHCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHH
VHRHPARQAIVRGVARICEELDIPVVAEGVEELDEARCLSDAGIRLMQGFLFCRPLFEGV
HHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GEAATLAWPALAPQAGGRQ
CCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]