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The map label for this gene is paaK [H]

Identifier: 119896594

GI number: 119896594

Start: 327612

End: 328934

Strand: Direct

Name: paaK [H]

Synonym: azo0303

Alternate gene names: 119896594

Gene position: 327612-328934 (Clockwise)

Preceding gene: 119896593

Following gene: 119896595

Centisome position: 7.49

GC content: 65.91

Gene sequence:

>1323_bases
ATGCCCATCAACAGCTATGCACCCCCGCAGCTCGAACCGATCGAGACCGCGAGCATCGACGAGCTGCGCGCGCTGCAGCT
CGAGCGCCTGAAGTGGAGCGTGCGCCACGCCTACGAGAACGTGCCGCACTACCGCAAGAAGTTCGACGAGAAGGGCGTCC
ATCCGGACGACCTCAAGTCCCTCGCCGACCTCGCCAAGTTCCCCTTCACCAGCAAGTATGACCTGCGCGACAACTACCCC
TTCGGCATGTTCGCGGTGCCGATGGACAGGATCGCCCGCGTCCACGCCTCGTCCGGCACCACCGGCAAGCCGACCGTGGT
GGGCTACACGCTGAAGGACATCGACACCTGGGCCACCGTGGTGGCGCGCTCGATCCGCGCCGCGGGCGGACGCCCCGGTG
ACATGGTGCACGTGTCCTACGGCTACGGCCTCTTCACCGGCGGCCTGGGGGCGCACTACGGCGCCGAGAAGCTGGGCTGC
ACCGTGGTGCCGATGTCCGGCGGCCAGACCGAGAAGCAGATCCAGATCATCCAGGACTTCAAGCCCAAGATCATCATGGT
GACGCCCTCCTACATGCTGACCATCCTCGACGAGATGGAACGCATGGGCATCGACCCGAAATCCACCTCGCTGCGCATCG
GCATCTTCGGCGCCGAGCCGTGGACGCCGGCGATGCGCGTCGCGATGGAGCAGCGCGCCGGCATCGACGCGGTGGACATC
TACGGCCTGTCCGAAGTGATGGGGCCGGGCGTGGCCAATGAATGCGTCGAGACAAAGGACGGCCCGACCATCTGGGAAGA
CCACTTCTACCCCGAGATCATCGACCCCAATACCGGCGAGGTGCTGCCGGACGGTTCCGAAGGCGAGCTGGTGTTCACCT
CGCTCTCCAAGGAAGCGATGCCGGTGATCCGCTACCGCACCCGCGACCTCACCCGGCTGCTGCCGCCCAGCGCGCGCAGC
ATGCGGCGCATGGCCAAGATCACCGGCCGTTCGGACGACATGCTGATCATCCGCGGTGTGAACGTGTTCCCGACCCAGAT
CGAGGAACTGATCTGCAAGATCCCCAAGCTCGCGCCGCACTACCTGCTGGAGGTCGACAAGCAGGGCCACATGGACACGC
TGACGGTGAAGGTGGAGATCAATGCCGAGGCCGAGGTGGGCCGTCACCCCGAGCACAAGGAAGCGCTGGCGCGCGAGCTG
ACGCACTCGATCAAGAGCCTGATCGGGGTGTCCGCAAAGGTGCTGGTCGGCGAGCCGTTCTCGATCGAGCGGGTCACGGT
GGGCAAGGCCAAGCGCGTCATCGACCGCCGGCCCAAGGACTGA

Upstream 100 bases:

>100_bases
GCCGGTCGTAGAAATACAGGCCTGAACGGCCGCCGCCCGGACAACAAAAAACAGCCTGCCGGGCGGCTCCAGAACCAGCA
GAGGGACAGAGGAGACAACC

Downstream 100 bases:

>100_bases
ATTTCCGACGGCGGCCCGTGTAGCCGCCGCGTTTGACACCACAAGGAGGAGCGCAGCATGTACACCCAAGCGCTGGACAT
ACCCCAGGCCGACGCCGCGC

Product: phenylacetate--CoA ligase

Products: NA

Alternate protein names: Phenylacetyl-CoA ligase; PA-CoA ligase [H]

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MPINSYAPPQLEPIETASIDELRALQLERLKWSVRHAYENVPHYRKKFDEKGVHPDDLKSLADLAKFPFTSKYDLRDNYP
FGMFAVPMDRIARVHASSGTTGKPTVVGYTLKDIDTWATVVARSIRAAGGRPGDMVHVSYGYGLFTGGLGAHYGAEKLGC
TVVPMSGGQTEKQIQIIQDFKPKIIMVTPSYMLTILDEMERMGIDPKSTSLRIGIFGAEPWTPAMRVAMEQRAGIDAVDI
YGLSEVMGPGVANECVETKDGPTIWEDHFYPEIIDPNTGEVLPDGSEGELVFTSLSKEAMPVIRYRTRDLTRLLPPSARS
MRRMAKITGRSDDMLIIRGVNVFPTQIEELICKIPKLAPHYLLEVDKQGHMDTLTVKVEINAEAEVGRHPEHKEALAREL
THSIKSLIGVSAKVLVGEPFSIERVTVGKAKRVIDRRPKD

Sequences:

>Translated_440_residues
MPINSYAPPQLEPIETASIDELRALQLERLKWSVRHAYENVPHYRKKFDEKGVHPDDLKSLADLAKFPFTSKYDLRDNYP
FGMFAVPMDRIARVHASSGTTGKPTVVGYTLKDIDTWATVVARSIRAAGGRPGDMVHVSYGYGLFTGGLGAHYGAEKLGC
TVVPMSGGQTEKQIQIIQDFKPKIIMVTPSYMLTILDEMERMGIDPKSTSLRIGIFGAEPWTPAMRVAMEQRAGIDAVDI
YGLSEVMGPGVANECVETKDGPTIWEDHFYPEIIDPNTGEVLPDGSEGELVFTSLSKEAMPVIRYRTRDLTRLLPPSARS
MRRMAKITGRSDDMLIIRGVNVFPTQIEELICKIPKLAPHYLLEVDKQGHMDTLTVKVEINAEAEVGRHPEHKEALAREL
THSIKSLIGVSAKVLVGEPFSIERVTVGKAKRVIDRRPKD
>Mature_439_residues
PINSYAPPQLEPIETASIDELRALQLERLKWSVRHAYENVPHYRKKFDEKGVHPDDLKSLADLAKFPFTSKYDLRDNYPF
GMFAVPMDRIARVHASSGTTGKPTVVGYTLKDIDTWATVVARSIRAAGGRPGDMVHVSYGYGLFTGGLGAHYGAEKLGCT
VVPMSGGQTEKQIQIIQDFKPKIIMVTPSYMLTILDEMERMGIDPKSTSLRIGIFGAEPWTPAMRVAMEQRAGIDAVDIY
GLSEVMGPGVANECVETKDGPTIWEDHFYPEIIDPNTGEVLPDGSEGELVFTSLSKEAMPVIRYRTRDLTRLLPPSARSM
RRMAKITGRSDDMLIIRGVNVFPTQIEELICKIPKLAPHYLLEVDKQGHMDTLTVKVEINAEAEVGRHPEHKEALARELT
HSIKSLIGVSAKVLVGEPFSIERVTVGKAKRVIDRRPKD

Specific function: Catalyzes the activation of phenylacetic acid to phenylacetyl-CoA [H]

COG id: COG1541

COG function: function code H; Coenzyme F390 synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787664, Length=429, Percent_Identity=65.967365967366, Blast_Score=602, Evalue=1e-173,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011880 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.30 [H]

Molecular weight: Translated: 48937; Mature: 48806

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPINSYAPPQLEPIETASIDELRALQLERLKWSVRHAYENVPHYRKKFDEKGVHPDDLKS
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHH
LADLAKFPFTSKYDLRDNYPFGMFAVPMDRIARVHASSGTTGKPTVVGYTLKDIDTWATV
HHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHH
VARSIRAAGGRPGDMVHVSYGYGLFTGGLGAHYGAEKLGCTVVPMSGGQTEKQIQIIQDF
HHHHHHHCCCCCCCEEEEEECCEEEECCCCCCCCHHHHCEEEEECCCCCCHHHHHHHHCC
KPKIIMVTPSYMLTILDEMERMGIDPKSTSLRIGIFGAEPWTPAMRVAMEQRAGIDAVDI
CCCEEEECHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEEC
YGLSEVMGPGVANECVETKDGPTIWEDHFYPEIIDPNTGEVLPDGSEGELVFTSLSKEAM
CCHHHHHCCCHHHHHHCCCCCCCEECCCCCCCEECCCCCCCCCCCCCCCEEEEECCCHHH
PVIRYRTRDLTRLLPPSARSMRRMAKITGRSDDMLIIRGVNVFPTQIEELICKIPKLAPH
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCH
YLLEVDKQGHMDTLTVKVEINAEAEVGRHPEHKEALARELTHSIKSLIGVSAKVLVGEPF
HHEEECCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
SIERVTVGKAKRVIDRRPKD
CEEEEEHHHHHHHHHCCCCC
>Mature Secondary Structure 
PINSYAPPQLEPIETASIDELRALQLERLKWSVRHAYENVPHYRKKFDEKGVHPDDLKS
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHH
LADLAKFPFTSKYDLRDNYPFGMFAVPMDRIARVHASSGTTGKPTVVGYTLKDIDTWATV
HHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHH
VARSIRAAGGRPGDMVHVSYGYGLFTGGLGAHYGAEKLGCTVVPMSGGQTEKQIQIIQDF
HHHHHHHCCCCCCCEEEEEECCEEEECCCCCCCCHHHHCEEEEECCCCCCHHHHHHHHCC
KPKIIMVTPSYMLTILDEMERMGIDPKSTSLRIGIFGAEPWTPAMRVAMEQRAGIDAVDI
CCCEEEECHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEEC
YGLSEVMGPGVANECVETKDGPTIWEDHFYPEIIDPNTGEVLPDGSEGELVFTSLSKEAM
CCHHHHHCCCHHHHHHCCCCCCCEECCCCCCCEECCCCCCCCCCCCCCCEEEEECCCHHH
PVIRYRTRDLTRLLPPSARSMRRMAKITGRSDDMLIIRGVNVFPTQIEELICKIPKLAPH
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCH
YLLEVDKQGHMDTLTVKVEINAEAEVGRHPEHKEALARELTHSIKSLIGVSAKVLVGEPF
HHEEECCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
SIERVTVGKAKRVIDRRPKD
CEEEEEHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]